BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	464,325	G→C	6.8%	H259D (CAC→GAC) ‡	davB ←	lysine 2‑monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	464,325	0	G	C	6.8%	1136.6 / 53.2	339	H259D (CAC→GAC) ‡	davB	lysine 2‑monooxygenase
Reads supporting (aligned to +/- strand): ref base G (144/172); new base C (10/13); total (154/185)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 346 total aligned reads displayed.

GGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAGGTGG  >  NC_002947/464176‑464474

ggcggcGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTg                                                                                                                                                       <  1:2723295/150‑1 (MQ=255)

 gcggcgCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTg                                                                                                                                                       <  1:3250668/149‑1 (MQ=255)

    gcgcGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtgg                                                                                                                                                   <  1:4681301/150‑1 (MQ=255)

    gcgcGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtgg                                                                                                                                                   <  2:5357103/150‑1 (MQ=255)

     cgcgGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtggt                                                                                                                                                  <  2:2622948/150‑1 (MQ=255)

      gcgGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtggtg                                                                                                                                                 <  2:1206007/150‑1 (MQ=255)

      gcgGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtggtg                                                                                                                                                 <  2:486305/150‑1 (MQ=255)

            aaTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGgtcgtc                                                                                                                                           <  2:6672174/150‑1 (MQ=255)

            aaTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTGGTGGCAGTGGTGCCCCGCCCCCGACCACCAGGTGCTGGTGgtcgtc                                                                                                                                           <  2:5236912/150‑1 (MQ=255)

             aTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGtcgtcg                                                                                                                                          <  2:2500390/150‑1 (MQ=255)

             aTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcgggg                                                                                                                                          <  2:6295177/150‑4 (MQ=255)

             aTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTCGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGtcgtcg                                                                                                                                          <  2:4810784/150‑1 (MQ=255)

                 gcTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGt                                                                                                                                      >  1:839940/1‑150 (MQ=255)

                 gcTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGt                                                                                                                                      >  1:6724397/1‑150 (MQ=255)

                  cTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGtt                                                                                                                                     <  2:2264620/150‑1 (MQ=255)

                   ttGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTg                                                                                                                                    <  2:4694735/150‑1 (MQ=255)

                    tGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTgg                                                                                                                                   <  1:2426183/150‑1 (MQ=255)

                        cccGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGtcat                                                                                                                               >  2:3489503/1‑150 (MQ=255)

                              tcgcgCTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATcaccac                                                                                                                         >  2:1410113/2‑150 (MQ=255)

                                    tGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGaa                                                                                                                   <  1:3884463/150‑1 (MQ=255)

                                    tGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGaa                                                                                                                   <  2:3310374/150‑1 (MQ=255)

                                            cATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCa                                                                                                           <  2:6157257/150‑1 (MQ=255)

                                            cATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCa                                                                                                           <  2:3076755/150‑1 (MQ=255)

                                            cATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCa                                                                                                           >  1:481268/1‑150 (MQ=255)

                                             aTGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAg                                                                                                          >  1:3416300/1‑150 (MQ=255)

                                             aTGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAg                                                                                                          >  1:609203/1‑150 (MQ=255)

                                              tGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGc                                                                                                         >  2:238920/1‑150 (MQ=255)

                                                 aGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATc                                                                                                      <  2:4076175/150‑1 (MQ=255)

                                                  gCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCg                                                                                                     >  1:2960621/1‑150 (MQ=255)

                                                   cGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGa                                                                                                    <  1:4457370/150‑1 (MQ=255)

                                                    gTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAg                                                                                                   >  1:3559430/1‑150 (MQ=255)

                                                     tGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGt                                                                                                  <  2:5861843/150‑1 (MQ=255)

                                                     tGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGt                                                                                                  >  2:1736972/1‑150 (MQ=255)

                                                      gCTCGGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGtt                                                                                                 <  1:6630081/150‑1 (MQ=255)

                                                      gCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGtt                                                                                                 >  1:2189459/1‑150 (MQ=255)

                                                        tCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTgg                                                                                               <  1:1961938/150‑1 (MQ=255)

                                                        tCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTgg                                                                                               <  2:3857567/150‑1 (MQ=255)

                                                        tCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTgg                                                                                               >  2:4640994/1‑150 (MQ=255)

                                                         cAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTggg                                                                                              <  1:5226874/150‑1 (MQ=255)

                                                               ggTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTc                                                                                        <  1:5548791/150‑1 (MQ=255)

                                                                gTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTcc                                                                                       <  2:3609920/150‑1 (MQ=255)

                                                                  gCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCCCCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGa                                                                                     <  1:54091/150‑1 (MQ=255)

                                                                   cccTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAg                                                                                    >  1:1894373/1‑150 (MQ=255)

                                                                     ctctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTc                                                                                  >  1:6073961/1‑150 (MQ=255)

                                                                     ctctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGCGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTc                                                                                  >  2:749002/1‑150 (MQ=255)

                                                                      tctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGtcgtcg                                                                                                                                          >  1:2326281/1‑93 (MQ=255)

                                                                      tctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTcc                                                                                 >  1:3108978/1‑150 (MQ=255)

                                                                      tctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTcc                                                                                 >  1:6326372/1‑150 (MQ=255)

                                                                      tctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTcc                                                                                 <  2:3950608/150‑1 (MQ=255)

                                                                        tGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCa                                                                               >  1:5693988/1‑150 (MQ=255)

                                                                          gCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAc                                                                             >  1:936015/1‑150 (MQ=255)

                                                                          gCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAc                                                                             >  1:4042336/1‑150 (MQ=255)

                                                                          gCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAc                                                                             >  2:6006598/1‑150 (MQ=255)

                                                                            cAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAccg                                                                           >  1:1395664/1‑150 (MQ=255)

                                                                             aaTGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAccgc                                                                          >  2:4799189/1‑150 (MQ=255)

                                                                             aaTGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAccgc                                                                          >  1:422323/1‑150 (MQ=255)

                                                                              atccACGCAGCGTACCGGTACGTCGCGCCAGAGGCCTTGTGGCACTAGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGTGGAAGTCCGAGTCCCAAccgcc                                                                         <  2:5049909/147‑1 (MQ=255)

                                                                                  aCGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTg                                                                     >  1:4079419/1‑150 (MQ=255)

                                                                                   cGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGc                                                                    <  1:5009256/150‑1 (MQ=255)

                                                                                   cGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGc                                                                    <  1:2576147/150‑1 (MQ=255)

                                                                                         gTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAAc                                                              <  2:3238117/150‑1 (MQ=255)

                                                                                             cGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGAc                                                          <  2:1311953/150‑1 (MQ=255)

                                                                                                    tCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCg                                                   >  1:256424/1‑150 (MQ=255)

                                                                                                     cgcgCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCGGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGa                                                  <  2:28280/150‑1 (MQ=255)

                                                                                                           aGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACtt                                            >  2:1853410/1‑150 (MQ=255)

                                                                                                           aGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACtt                                            <  2:609203/150‑1 (MQ=255)

                                                                                                             aTGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTcg                                          >  2:5171973/1‑150 (MQ=255)

                                                                                                             aTGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTcg                                          >  1:587718/1‑150 (MQ=255)

                                                                                                              tGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCACAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTcgc                                         <  2:6421969/150‑1 (MQ=255)

                                                                                                               gCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTcgcg                                        >  1:4020736/1‑150 (MQ=255)

                                                                                                                gggtgtgGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCgg                                       >  1:695598/4‑150 (MQ=255)

                                                                                                                ccTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCgg                                       <  2:6845549/150‑1 (MQ=255)

                                                                                                                  ttGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTg                                     >  2:1348472/1‑150 (MQ=255)

                                                                                                                      ggCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTgg                                 <  1:3594117/150‑1 (MQ=255)

                                                                                                                        cACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGaa                               <  1:1119559/150‑1 (MQ=255)

                                                                                                                        cACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGaa                               <  1:6490205/150‑1 (MQ=255)

                                                                                                                         aCTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGGCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCGGGAAg                              >  1:4742374/1‑150 (MQ=255)

                                                                                                                            tGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTc                           <  2:4486747/150‑1 (MQ=255)

                                                                                                                            tGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTc                           <  1:4642702/150‑1 (MQ=255)

                                                                                                                                ccACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCt                       >  2:6546993/1‑150 (MQ=255)

                                                                                                                                ccACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCt                       >  2:5638894/1‑150 (MQ=255)

                                                                                                                                ccACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCt                       <  2:2157841/150‑1 (MQ=255)

                                                                                                                                 cACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCtt                      >  2:2920055/1‑150 (MQ=255)

                                                                                                                                 cACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCtt                      <  2:6612343/150‑1 (MQ=255)

                                                                                                                                  aCGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTg                     >  2:4703525/1‑150 (MQ=255)

                                                                                                                                  aCGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTg                     >  2:5697079/1‑150 (MQ=255)

                                                                                                                                      ccccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaa                 >  2:4986095/1‑150 (MQ=255)

                                                                                                                                      ccccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaa                 <  2:6499047/150‑1 (MQ=255)

                                                                                                                                       gacGACCACCAGCACCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaaa                >  1:3164654/3‑150 (MQ=255)

                                                                                                                                       cccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaaa                >  1:2683529/1‑150 (MQ=255)

                                                                                                                                       cccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaaa                >  1:1637059/1‑150 (MQ=255)

                                                                                                                                          gACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaaaata             <  1:5764352/150‑3 (MQ=255)

                                                                                                                                          gACCACCAGCACCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGc                                    <  1:1437756/127‑1 (MQ=255)

                                                                                                                                          gACCACCAGCACCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGc                                    >  2:1437756/1‑127 (MQ=255)

                                                                                                                                              accaGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAgcgc         >  1:1494661/1‑150 (MQ=255)

                                                                                                                                                caGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGa       <  1:6530747/150‑1 (MQ=255)

                                                                                                                                                 aGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAg      <  1:4570797/150‑1 (MQ=255)

                                                                                                                                                  ggTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAgg     >  1:3022713/1‑150 (MQ=255)

                                                                                                                                                    tGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAGGTg   >  2:201630/1‑150 (MQ=255)

                                                                                                                                                     gCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAGGTgg  <  2:1655379/150‑1 (MQ=255)

GGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAGGTGG  >  NC_002947/464176‑464474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`