Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,745,455 C→G 6.3% intergenic (+15/+56) hutF → / ← PP_5037 formiminoglutamate deiminase/lipocalin family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,745,4550CG 6.3% 368.8 / 2.3 122intergenic (+15/+56)hutF/PP_5037formiminoglutamate deiminase/lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (10/103);  new base G (3/6);  total (13/109)
Fisher's exact test for biased strand distribution p-value = 5.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

CTGTTTGCCGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCA  >  NC_002947/5745306‑5745604
                                                                                                                                                      |                                                                                                                                                        
cTGTTTTCCGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGgtt‑gc                                                                                                                                                          <  1:3139814/150‑1 (MQ=255)
 tGTTTGCCGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGgtt‑gcg                                                                                                                                                         <  1:4386847/150‑1 (MQ=255)
  gTTTGCCGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGgtt‑gcg‑t                                                                                                                                                       <  1:6151208/150‑1 (MQ=255)
    ttGCCGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGgtt‑gcg‑ttg                                                                                                                                                     <  1:5418070/150‑1 (MQ=255)
    ttGCCGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGgtt‑gcg‑ttg                                                                                                                                                     <  2:3117770/150‑1 (MQ=255)
    ttGCCGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGgtt‑gcg‑ttg                                                                                                                                                     <  1:4382889/150‑1 (MQ=255)
     tGCCGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGgtt‑gcg‑ttgc                                                                                                                                                    <  1:1381694/150‑1 (MQ=255)
      gCCGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCa                                                                                                                                                   <  2:1181207/150‑1 (MQ=255)
      gCCGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCa                                                                                                                                                   <  1:5502361/150‑1 (MQ=255)
      gCCGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCa                                                                                                                                                   <  1:1359127/150‑1 (MQ=255)
      gCCGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCa                                                                                                                                                   <  2:6657108/150‑1 (MQ=255)
         ggTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGgtt‑gcg‑t                                                                                                                                                       <  2:6336417/143‑1 (MQ=255)
           tGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCt                                                                                                                                              <  2:2721050/150‑1 (MQ=255)
             gCGATCGCCAGGTGCTCGATGTGATGGTGTATGGGCAATGGGTGGTGCGCCAGGGGCGGCATTCCCAGGAAGATGACAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTc                                                                                                                                            <  2:4811460/150‑1 (MQ=255)
               gATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑TCG‑TTGGAGCCCATcgc                                                                                                                                          >  2:5362461/1‑150 (MQ=255)
               gATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GGG‑GTGGAGcccaaacc                                                                                                                                          >  2:5736687/1‑145 (MQ=255)
               gATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTcgc                                                                                                                                          <  2:1375651/150‑1 (MQ=255)
               gATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTATTGGGCTGAGCGTACCGGGGTTGGCG‑TT‑CAGcccaaagc                                                                                                                                          >  2:2781524/1‑145 (MQ=255)
                 tCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGt                                                                                                                                        <  1:1201320/150‑1 (MQ=255)
                 tCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGt                                                                                                                                        <  2:4488618/150‑1 (MQ=255)
                  cGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTg                                                                                                                                       <  2:6086494/150‑1 (MQ=255)
                    ccAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCa                                                                                                                                     >  1:2536277/1‑150 (MQ=255)
                     cAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCaa                                                                                                                                    <  1:2923544/150‑1 (MQ=255)
                     cAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGGGctt                                                                                                                                    <  2:1726416/150‑3 (MQ=255)
                     cAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCGCGGGctt                                                                                                                                    <  1:640935/150‑3 (MQ=255)
                         tGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTTCGGCCCATCGCGTGCAA‑Gccc                                                                                                                               >  2:2249613/1‑150 (MQ=255)
                         tGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑Gccc                                                                                                                               <  1:876956/150‑1 (MQ=255)
                          gcgcGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCcg                                                                                                                              <  1:4224897/150‑1 (MQ=255)
                            gcgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGGGCTT‑GCAcgcg                                                                                                                            <  2:6559000/150‑1 (MQ=255)
                            gcgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGGGCTT‑GCAcgcg                                                                                                                            <  2:5118731/150‑1 (MQ=255)
                            gcgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGGGCTT‑GCAcgcg                                                                                                                            <  2:6559010/150‑1 (MQ=255)
                              gATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGaa                                                                                                                          <  2:3353620/150‑1 (MQ=255)
                              gATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGaa                                                                                                                          <  1:3463395/150‑1 (MQ=255)
                              gATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGGGCTT‑GCACGCGaa                                                                                                                          <  2:1897404/150‑1 (MQ=255)
                              gATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGaa                                                                                                                          <  1:3169616/150‑1 (MQ=39)
                              gATGTGATGGTGAAGGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCA‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGaa                                                                                                                          <  2:2642100/150‑1 (MQ=255)
                               ctgtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGAAg                                                                                                                         <  2:1186967/149‑1 (MQ=39)
                               aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGTAA‑GCCCGCGAAg                                                                                                                         <  1:6308254/150‑1 (MQ=255)
                               aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAg                                                                                                                         <  1:2713158/150‑1 (MQ=255)
                               aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAg                                                                                                                         <  2:1614170/150‑1 (MQ=255)
                               aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAg                                                                                                                         <  1:6056729/150‑1 (MQ=255)
                               aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAg                                                                                                                         <  2:5886638/150‑1 (MQ=255)
                               aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGGGCTT‑GCACGCGAAg                                                                                                                         <  2:2181526/150‑1 (MQ=255)
                               aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGAAg                                                                                                                         <  2:6259724/150‑1 (MQ=39)
                               aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGAAg                                                                                                                         <  2:3896181/150‑1 (MQ=39)
                               aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGAAg                                                                                                                         <  1:5356669/150‑1 (MQ=39)
                               aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGAAg                                                                                                                         <  1:918300/150‑1 (MQ=39)
                                tgtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGGGCTT‑GCACGCGAAgg                                                                                                                        <  1:3254758/150‑1 (MQ=255)
                                  tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGt                                                                                                                      <  1:829443/150‑1 (MQ=255)
                                  tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGt                                                                                                                      <  1:1929516/150‑1 (MQ=255)
                                  tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGAAgggc                                                                                                                      <  2:2387041/150‑2 (MQ=39)
                                  tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGAAgggc                                                                                                                      <  2:3467845/150‑2 (MQ=39)
                                   gctGGTGAATGGGCACTGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGAAgggct                                                                                                                     <  1:2461652/148‑3 (MQ=38)
                                   gATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGtt                                                                                                                     <  1:4076772/150‑1 (MQ=255)
                                   gATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGtt                                                                                                                     <  2:4149516/150‑1 (MQ=255)
                                   gATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGtt                                                                                                                     <  1:3234471/150‑1 (MQ=255)
                                    aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTg                                                                                                                    <  2:4674788/150‑1 (MQ=255)
                                    aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTg                                                                                                                    <  1:2016480/150‑1 (MQ=255)
                                      ggTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGGCCGTCGCGGGCCA‑GCCCGCGAAGGGGTgca                                                                                                                  >  2:6184048/1‑150 (MQ=255)
                                      ggTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGAAg                                                                                                                         <  1:3694673/143‑1 (MQ=39)
                                      ggTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGAAg                                                                                                                         >  2:3694673/1‑143 (MQ=39)
                                        tGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTgcaa‑c                                                                                                               <  1:4610857/150‑1 (MQ=255)
                                          aaTGGGCAATGGGGGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTGTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTgcaa‑cgc                                                                                                             <  2:6223204/150‑1 (MQ=255)
                                                     ggtggtGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACTGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGAAg                                                                                                                         >  2:535391/1‑128 (MQ=38)
                                                     ggtggtGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACTGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGAAg                                                                                                                         <  1:535391/128‑1 (MQ=38)
                                                         gtgGGCCTGGGGCGGCATGCCCTGGAAGATGAATGGGGGGGGGGGTGTGCCTTGGTGTTGCGCCAGCTTTTGGGCTGTGGGTACGGGGGTT‑GCG‑TTGCAGCCCTTCGGGTGCAA‑GCCCGGGATGGGTTGCAA‑CGCAACCCCAAAAAACTc                                                                                              <  2:6811989/150‑1 (MQ=255)
                                                          tgCGCCAGGGGCGGCTTGCCCAGGAAGTTGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTGGGGCTGAGCGTACGGGGGTT‑GCG‑TTCCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCa                                                                                             <  2:6647616/150‑1 (MQ=255)
                                                            cgcCTGGGGCGGATTGCCCTGGAAGATGAACGCGGGCGGGGGTTGGCCACGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACGGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGGGAAGGTTTGAAA‑CGCAACCCCAAAAAACTCAGt                                                                                           <  2:456389/150‑1 (MQ=255)
                                                            cgcCAGGGGCGGCATGCCCAGGAAGATGAACGCGGGCGGGCGTTTGCCATGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGt                                                                                           <  2:5600452/150‑1 (MQ=255)
                                                                aGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGcc                                                                                                                                                >  2:6830825/1‑95 (MQ=255)
                                                                     gggctggCCCTGGACGTTGAACGGGGGGGGGCGTTTGCCATGGTGTGGCGCCTGTTTTTGGGCGGCGCGTCCGGGGGTT‑GGG‑TGGCCGCCCTTCGCGTGCAC‑GCCCGCGATGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTa                                                                                  <  1:3919051/144‑1 (MQ=255)
                                                                                    gATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCGCGGGCTT‑GCACGCGAAGGGCTgcaa‑c                                                                                                               <  2:4905750/106‑1 (MQ=25)
                                                                                           gcgggcggGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGtt                                                                                                                                       >  1:662607/1‑76 (MQ=255)
                                                                                              ggggggcgTTTGCCACGGTGTTGCGCCAGCTTTGGGGCTGAGCGTACCGGGGTG‑GCG‑TTGCAGCCCTCCGCGTGCAC‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCa                                                         <  2:4875822/147‑1 (MQ=255)
                                                                                                 gggcgCTCGCCACGGTGGTCCGCCAGCTTTTGGGCGGTGCGTCCCGGGGTG‑GCG‑TGCCAGCCCTCCGCGTGCAC‑GCCCGCGAAGGTTCCCAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGAt                                                      <  1:6705665/150‑1 (MQ=255)
                                                                                                      tttGCCACGGTGTTGCGCCCGCTTTTGGGCTGTGCGTCCCGGGGTG‑GCG‑TTGCAGCCCTCCGCGTGCAC‑GCCCGCGATGGGTGGCCA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                 <  2:3192446/150‑1 (MQ=255)
                                                                                                           catggTGTGGCGCCAGCTTTGGGGCTGAGCGTACGGGGGTT‑GCG‑TGGCAGCCCTTCGGTTGCAA‑GCCCGCGACGGGTGGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTg                                            <  2:5813369/147‑1 (MQ=255)
                                                                                                             tggTGTGGCGCCAGCTTTTGGGCTGCGCGTACGGGGGTT‑GCT‑TGGCAGCCCTTCGCTTGCAC‑GCCCGCTAGGGTTCGCAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGgt                                          <  1:518598/149‑1 (MQ=255)
                                                                                                             aGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGgt                                          <  2:6644608/150‑1 (MQ=255)
                                                                                                             aGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGgt                                          <  2:5062013/150‑1 (MQ=255)
                                                                                                             aGGTGTTGCGCCAGCTTTTGGGCTGAGCGGACCGGGGTT‑GCG‑TTGCAGCCGTTCGCGTGCAA‑GCCCGCGAAGGTTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGgt                                          <  2:3879032/150‑1 (MQ=255)
                                                                                                                    gcgcacgCTTTTGGGCTGAGCGTCCGGGGGTT‑GCG‑TGGCCGCCCTGCGCGTGCAA‑GCCCGCTACGGGTTGCAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAc                                   <  1:6733353/144‑1 (MQ=255)
                                                                                                                     cgcCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAcc                                  <  2:2187860/150‑1 (MQ=255)
                                                                                                                       cctgCTTTTGGGGTGAGCGTACGGGGGTT‑GCG‑TTGCAGCCCTGCGCGTGCAA‑GCCCGCTATGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAcccc                                <  2:3055532/147‑1 (MQ=255)
                                                                                                                        cAGCTTTTGGGCTGAGCGTCCCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAC‑CCCCGCGACGGGTTGAAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCct                               <  2:3142643/150‑1 (MQ=255)
                                                                                                                                gggCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAC‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                       <  2:4312844/150‑1 (MQ=255)
                                                                                                                                gggCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAC‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                       <  2:1313744/150‑1 (MQ=255)
                                                                                                                                     gAGCGTACGGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGATGGGTTGAAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt                  <  1:512179/150‑1 (MQ=255)
                                                                                                                                      aGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTGGCGTGCAA‑GCCCGCGAAGGGTTGAAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg                 <  2:3088112/150‑1 (MQ=255)
                                                                                                                                       tggttccGGGGTT‑GGGTTTGCAGCCTTTCG‑GTTCAACGCCCG‑GTTTGGTTTCAACCGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg                 <  1:1877688/145‑1 (MQ=255)
                                                                                                                                        cGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTGCAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGCTCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGa               <  2:3304167/150‑1 (MQ=255)
                                                                                                                                          gacgggggTT‑GGG‑TTGCAGCCCTTCGGGTGCAA‑GCCCGCGATGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAca             <  1:5663726/146‑1 (MQ=255)
                                                                                                                                            ccGGGGTT‑GCG‑TTGCAGCCCTTCGCTTGCAA‑GCCCGCTACGGGTTCAAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagc           <  2:1941188/150‑1 (MQ=255)
                                                                                                                                            ccGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagc           <  2:5338887/150‑1 (MQ=255)
                                                                                                                                             gggggTT‑GGG‑TTGCCCCCGTTGGGGTCCAC‑CCCCGCGATGGTTTCCAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca          <  1:6514349/149‑1 (MQ=255)
                                                                                                                                              ggggTT‑GCG‑TTGCAGCCCTTCGCGTGCAC‑GCCCGCGATGGGTTGAAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagcag         <  1:3241440/150‑1 (MQ=255)
                                                                                                                                                  tt‑gcg‑ttgcAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc     >  2:560303/1‑150 (MQ=255)
                                                                                                                                                  tt‑gcg‑ttgcAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc     >  1:3568982/1‑150 (MQ=255)
                                                                                                                                                      cg‑ttgcCGCCCTTCGCGCGCAC‑CCCCGCGACGGGTGGAAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa  <  2:295670/150‑1 (MQ=255)
                                                                                                                                                      cg‑ttgcAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa  <  2:3402470/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                        
CTGTTTGCCGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCGCGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCA  >  NC_002947/5745306‑5745604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑