Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,745,462 G→A 17.2% intergenic (+22/+49) hutF → / ← PP_5037 formiminoglutamate deiminase/lipocalin family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,745,4620GA17.2% 263.8 / 44.4 123intergenic (+22/+49)hutF/PP_5037formiminoglutamate deiminase/lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (13/88);  new base A (3/17);  total (16/107)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-02

Only 100 of 127 total aligned reads displayed.

CGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCC  >  NC_002947/5745314‑5745611
                                                                                                                                                      |                                                                                                                                                        
cGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GGG‑GTGGAGc                                                                                                                                                         >  1:5719493/1‑150 (MQ=255)
  gTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGccc                                                                                                                                                       <  1:3155466/150‑1 (MQ=255)
   tGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCt                                                                                                                                                      <  2:2721050/150‑1 (MQ=255)
     gCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTc                                                                                                                                                    <  2:1029275/150‑1 (MQ=255)
       gATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTcg‑c                                                                                                                                                 <  1:6554868/150‑1 (MQ=255)
       gATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTcg‑c                                                                                                                                                 <  2:1375651/150‑1 (MQ=255)
       gATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTATTGGGCTGAGCGTACCGGGGTTGGCG‑TT‑CAGcccaaagc                                                                                                                                                  >  2:2781524/1‑145 (MQ=255)
         tCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGt                                                                                                                                               <  2:6448062/150‑1 (MQ=255)
         tCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGt                                                                                                                                               <  2:4488618/150‑1 (MQ=255)
         tCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGt                                                                                                                                               <  1:1201320/150‑1 (MQ=255)
           gCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGc                                                                                                                                             <  2:6203800/150‑1 (MQ=255)
            ccAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCa                                                                                                                                            >  1:2536277/1‑150 (MQ=255)
             cAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGGGctt                                                                                                                                           <  2:1726416/150‑3 (MQ=255)
             cAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGctt                                                                                                                                           <  1:640935/150‑3 (MQ=255)
               ggTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑Gc                                                                                                                                        <  1:2561540/150‑1 (MQ=255)
                 tGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTTCGGCCCATCG‑CGTGCAA‑Gccc                                                                                                                                      >  2:2249613/1‑150 (MQ=255)
                 tGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑Gccc                                                                                                                                      <  1:4309074/150‑1 (MQ=255)
                  gcgcGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCcg                                                                                                                                     <  1:4224897/150‑1 (MQ=255)
                    gcgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGGGCTT‑GCAcgcg                                                                                                                                   <  2:5118731/150‑1 (MQ=255)
                    gcgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGGGCTT‑GCAcgcg                                                                                                                                   <  2:6559010/150‑1 (MQ=255)
                    gcgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGGGCTT‑GCAcgcg                                                                                                                                   <  2:6559000/150‑1 (MQ=255)
                      gATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGaa                                                                                                                                 <  2:3353620/150‑1 (MQ=255)
                      gATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGaa                                                                                                                                 <  1:3463395/150‑1 (MQ=255)
                      gATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGGGCTT‑GCACGCGaa                                                                                                                                 <  2:1897404/150‑1 (MQ=255)
                      gATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGaa                                                                                                                                 <  1:3169616/150‑1 (MQ=39)
                      gATGTGATGGTGAAGGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCA‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGaa                                                                                                                                 <  2:2642100/150‑1 (MQ=255)
                       ctgtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAg                                                                                                                                <  2:1186967/149‑1 (MQ=39)
                       ctgtgAGGGTGAAGGGGCACTGGGTGGTGCGCCAGGGGCGGCAGGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGGT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAg                                                                                                                                <  2:6759854/149‑1 (MQ=38)
                       aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGTAA‑GCCCGCGAAg                                                                                                                                <  1:6308254/150‑1 (MQ=255)
                       aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAg                                                                                                                                <  2:1614170/150‑1 (MQ=255)
                       aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAg                                                                                                                                <  1:2713158/150‑1 (MQ=255)
                       aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAg                                                                                                                                <  2:5886638/150‑1 (MQ=255)
                       aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGGGCTT‑GCACGCGAAg                                                                                                                                <  2:2181526/150‑1 (MQ=255)
                       aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAg                                                                                                                                <  2:3896181/150‑1 (MQ=39)
                       aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAg                                                                                                                                <  1:918300/150‑1 (MQ=39)
                       aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAg                                                                                                                                <  1:3492453/150‑1 (MQ=39)
                       aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAg                                                                                                                                <  2:6259724/150‑1 (MQ=39)
                        tgtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGGGCTT‑GCACGCGAAgg                                                                                                                               <  1:3254758/150‑1 (MQ=255)
                        tgtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGTGCAA‑GCCCGCGAAgg                                                                                                                               <  2:2582709/150‑1 (MQ=255)
                          tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGt                                                                                                                             <  1:829443/150‑1 (MQ=255)
                          tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGt                                                                                                                             <  1:1929516/150‑1 (MQ=255)
                          tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAgggc                                                                                                                             <  2:3467845/150‑2 (MQ=39)
                           gctGGTGAATGGGCACTGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAgggct                                                                                                                            <  1:2461652/148‑3 (MQ=38)
                           gATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGtt                                                                                                                            <  1:3234471/150‑1 (MQ=255)
                           gATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGtt                                                                                                                            <  2:4149516/150‑1 (MQ=255)
                           gATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGtt                                                                                                                            <  1:4076772/150‑1 (MQ=255)
                            aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTg                                                                                                                           <  2:4674788/150‑1 (MQ=255)
                              ggTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGGCCGTCG‑CGGGCCA‑GCCCGCGAAGGGGTgca                                                                                                                         >  2:6184048/1‑150 (MQ=255)
                              ggTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAg                                                                                                                                >  2:3694673/1‑143 (MQ=39)
                              ggTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAg                                                                                                                                <  1:3694673/143‑1 (MQ=39)
                                tGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTgcaa‑c                                                                                                                      <  1:4610857/150‑1 (MQ=255)
                                tGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTgcaa‑c                                                                                                                      <  2:3522027/150‑1 (MQ=255)
                                tGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAGGGCTgcaa‑c                                                                                                                      <  1:164880/150‑1 (MQ=38)
                                  aaTGGGCAATGGGGGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTGTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTgcaa‑cgc                                                                                                                    <  2:6223204/150‑1 (MQ=255)
                                             ggtggtGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACTGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAg                                                                                                                                >  2:535391/1‑128 (MQ=38)
                                             ggtggtGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACTGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAg                                                                                                                                <  1:535391/128‑1 (MQ=38)
                                                 gtgGGCCTGGGGCGGCATGCCCTGGAAGATGAATGGGGGGGGGGGTGTGCCTTGGTGTTGCGCCAGCTTTTGGGCTGTGGGTACGGGGGTT‑GCG‑TTGCAGCCCTTCG‑GGTGCAA‑GCCCGGGATGGGTTGCAA‑CGCAACCCCAAAAAACTc                                                                                                     <  2:6811989/150‑1 (MQ=255)
                                                 gggcgcCTGGGGGGTCAGGCCCAGTACTATGAACGCGGGCGGGCTTTTGCCACGGTGTTGCGCATGCTTTGGGGCTTCGCGTACGGGGGTT‑GCG‑TCGACGCCGTTCG‑CGTGCAC‑GCCCGCGTCGGGTTGAAA‑CGCAACCCCAAAAAACTc                                                                                                     <  2:1921778/148‑1 (MQ=255)
                                                  tgCGCCAGGGGCGGCTTGCCCAGGAAGTTGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTGGGGCTGAGCGTACGGGGGTT‑GCG‑TTCCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCa                                                                                                    <  2:6647616/150‑1 (MQ=255)
                                                    cgcCTGGGGCGGATTGCCCTGGAAGATGAACGCGGGCGGGGGTTGGCCACGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACGGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGGGAAGGTTTGAAA‑CGCAACCCCAAAAAACTCAGt                                                                                                  <  2:456389/150‑1 (MQ=255)
                                                    cgcCAGGGGCGGCATGCCCAGGAAGATGAACGCGGGCGGGCGTTTGCCATGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGt                                                                                                  <  2:5600452/150‑1 (MQ=255)
                                                     gcCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GGG‑GTTGAGCCCCAAGCCGGGCAA‑CCCCCCCAAGGGTGGCAA‑CCAAACCCCaaaaaaaaaagg                                                                                                  >  2:6031636/1‑144 (MQ=255)
                                                        aGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGcc                                                                                                                                                        <  1:6830825/95‑1 (MQ=255)
                                                             gggctggCCCTGGACGTTGAACGGGGGGGGGCGTTTGCCATGGTGTGGCGCCTGTTTTTGGGCGGCGCGTCCGGGGGTT‑GGG‑TGGCCGCCCTTCG‑CGTGCAC‑GCCCGCGATGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTa                                                                                         <  1:3919051/144‑1 (MQ=255)
                                                                     ccAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTGGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGc                                                                                 <  2:5119977/150‑1 (MQ=255)
                                                                          aaGATGAACGCGGGGGGGCGTTTGCCACGGTGTTGCGCCTGCTTTTGGGCTGAGCGTACCGGGGTG‑GCG‑TGGCCGCCCTTCG‑CGTGCAC‑GCCCGCGACGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATc                                                                            <  2:4737678/150‑1 (MQ=255)
                                                                            gATGAACGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCgg                                                                          <  2:5494885/150‑1 (MQ=255)
                                                                            gATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAGGGCTgcaa‑c                                                                                                                      >  1:4905750/1‑106 (MQ=25)
                                                                            gATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAACCCTTCG‑CGGGCTT‑GCACGCGAAGGGCTgcaa‑c                                                                                                                      <  2:4905750/106‑1 (MQ=25)
                                                                                   gcgggcggGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGtt                                                                                                                                              <  2:662607/77‑2 (MQ=255)
                                                                                      ggggggcgTTTGCCACGGTGTTGCGCCAGCTTTGGGGCTGAGCGTACCGGGGTG‑GCG‑TTGCAGCCCTCCG‑CGTGCAC‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCa                                                                <  2:4875822/147‑1 (MQ=255)
                                                                                      ggcgggcgTTTGCAACGGTGTTGCGCCAGCTTTGGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCa                                                                <  2:4875882/150‑1 (MQ=255)
                                                                                         gggcgCTCGCCACGGTGGTCCGCCAGCTTTTGGGCGGTGCGTCCCGGGGTG‑GCG‑TGCCAGCCCTCCG‑CGTGCAC‑GCCCGCGAAGGTTCCCAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGAt                                                             <  1:6705665/150‑1 (MQ=255)
                                                                                              tttGCCACGGTGTTGCGCCCGCTTTTGGGCTGTGCGTCCCGGGGTG‑GCG‑TTGCAGCCCTCCG‑CGTGCAC‑GCCCGCGATGGGTGGCCA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                        <  2:3192446/150‑1 (MQ=255)
                                                                                              ttggCCAAGTTGTTTCGCCCGTTTTTGGGCTTAGCGTACCGGTGTT‑GCT‑TTGCAGCCATGCG‑CGTGAAA‑CCCCGCGATGTTTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                        <  2:4041669/147‑1 (MQ=255)
                                                                                                  ccACGGTGTTGCGCCCGCTTTGGGGCTGCGCGCCCGGGGGTT‑GCG‑TTGCAGCCCTGCG‑CGGGCAC‑GCCCGCGACGGGTGGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCt                                                    <  2:3067956/150‑1 (MQ=255)
                                                                                                   catggTGTGGCGCCAGCTTTGGGGCTGAGCGTACGGGGGTT‑GCG‑TGGCAGCCCTTCG‑GTTGCAA‑GCCCGCGACGGGTGGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTg                                                   <  2:5813369/147‑1 (MQ=255)
                                                                                                     tggTGTGGCGCCAGCTTTTGGGCTGCGCGTACGGGGGTT‑GCT‑TGGCAGCCCTTCG‑CTTGCAC‑GCCCGCTAGGGTTCGCAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGgt                                                 <  1:518598/149‑1 (MQ=255)
                                                                                                            gcgcacgCTTTTGGGCTGAGCGTCCGGGGGTT‑GCG‑TGGCCGCCCTGCG‑CGTGCAA‑GCCCGCTACGGGTTGCAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAc                                          <  1:6733353/144‑1 (MQ=255)
                                                                                                             cgcCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAcc                                         <  2:2187860/150‑1 (MQ=255)
                                                                                                               cctgCTTTTGGGGTGAGCGTACGGGGGTT‑GCG‑TTGCAGCCCTGCG‑CGTGCAA‑GCCCGCTATGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAcccc                                       <  2:3055532/147‑1 (MQ=255)
                                                                                                                cAGCTTTTGGGCTGAGCGTCCCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAC‑CCCCGCGACGGGTTGAAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCct                                      <  2:3142643/150‑1 (MQ=255)
                                                                                                                        ggggTGCGCGTCCGGGGTTT‑GGG‑TTGCAGCCGTTCG‑GGTCCAA‑GCCCGCGAGGGTTCCCAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                              <  1:5342635/150‑1 (MQ=255)
                                                                                                                        gggCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAC‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                              <  2:4312844/150‑1 (MQ=255)
                                                                                                                        gggCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAC‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                              <  2:1313744/150‑1 (MQ=255)
                                                                                                                           cTGAGCTTACGGGGTTT‑GCG‑TGCCCCCCCTCCG‑CGTCCAC‑CCCCGCAACGGGTACAAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGcc                           <  1:3702124/150‑1 (MQ=255)
                                                                                                                              aGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTGG‑CGTGCAA‑GCCCGCGAAGGGTTGAAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg                        <  2:3088112/150‑1 (MQ=255)
                                                                                                                               tggttccGGGGTT‑GGGTTTGCAGCCTTTCG‑‑GTTCAACGCCCG‑GTTTGGTTTCAACCGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg                        <  1:1877688/145‑1 (MQ=255)
                                                                                                                                ggTACGGGGTTG‑GGG‑TTGCAGCCCTTGG‑GTTGAAA‑GCCCGGTATGGGTTCAAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGa                      <  1:5462751/149‑1 (MQ=255)
                                                                                                                                cGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGCTCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGa                      <  2:3304167/150‑1 (MQ=255)
                                                                                                                                  gacgggggTT‑GGG‑TTGCAGCCCTTCG‑GGTGCAA‑GCCCGCGATGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAca                    <  1:5663726/146‑1 (MQ=255)
                                                                                                                                    ccGGGGTT‑GCG‑TTGCAGCCCTTCG‑CTTGCAA‑GCCCGCTACGGGTTCAAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagc                  <  2:1941188/150‑1 (MQ=255)
                                                                                                                                    ccGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagc                  <  2:5338887/150‑1 (MQ=255)
                                                                                                                                     gggggTT‑GGG‑TTGCCCCCGTTGG‑GGTCCAC‑CCCCGCGATGGTTTCCAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca                 <  1:6514349/149‑1 (MQ=255)
                                                                                                                                      ggggtg‑ggg‑tTGCCGCCCTTCG‑CGTGCAC‑GCCCGCGACGGGTTGCAC‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagcag                <  1:3241433/150‑1 (MQ=255)
                                                                                                                                       gggTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAgg               <  2:5697718/150‑1 (MQ=255)
                                                                                                                                          tt‑gcg‑ttgcAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc            >  2:560303/1‑150 (MQ=255)
                                                                                                                                              cg‑ttgcAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa         <  2:3402470/150‑1 (MQ=255)
                                                                                                                                               g‑ttgcAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCGGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc        >  2:1422568/1‑150 (MQ=255)
                                                                                                                                                gttcccgCCCTCGG‑CGTGCAC‑GCCCGCTTTGGGTCCCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc        <  1:6016330/144‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                        
CGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCG‑TTGCAGCCCTTCG‑CGTGCAA‑GCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCC  >  NC_002947/5745314‑5745611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑