Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,776,155 T→G 12.3% M605L (ATG→CTG)  betT‑I ← choline transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,776,1550TG12.3% 638.4 / 6.6 250M605L (ATG→CTG) betT‑Icholine transporter
Reads supporting (aligned to +/- strand):  ref base T (51/143);  new base G (38/2);  total (105/145)
Fisher's exact test for biased strand distribution p-value = 1.03e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

ATCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGCTTCTTTCAACGCCGGG  >  NC_002947/5776009‑5776303
                                                                                                                                                  |                                                                                                                                                    
 tCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCAGATAg                                                                                                                                                  >  2:5296280/1‑150 (MQ=255)
   gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGcc                                                                                                                                                <  2:6584569/150‑1 (MQ=255)
   gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGcc                                                                                                                                                <  1:2137615/150‑1 (MQ=255)
   gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGcc                                                                                                                                                >  2:4075527/1‑150 (MQ=255)
   gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCAGATAgac                                                                                                                                                >  1:6198796/1‑148 (MQ=255)
     gCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGt                                                                                                                                              <  2:3776116/150‑1 (MQ=255)
     gCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCAGATAGCCgg                                                                                                                                              >  2:3294564/1‑149 (MQ=255)
        caTCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCaa                                                                                                                                           <  1:4375254/150‑1 (MQ=255)
             cgcacgATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTc                                                                                                                                      <  2:2698956/150‑1 (MQ=255)
              gcacgATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCAAATATCCGGCAAGGCTCa                                                                                                                                     >  2:1654975/1‑150 (MQ=255)
              gcacgATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCCGAAAGCCGTCAAGGCGCa                                                                                                                                     >  2:6308813/1‑150 (MQ=255)
               cacgATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCAc                                                                                                                                    <  1:4861011/150‑1 (MQ=255)
                  gATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCTTGACGGCtatatgg                                                                                                                                 <  2:77965/150‑7 (MQ=255)
                   aTCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCg                                                                                                                                <  1:1729629/150‑1 (MQ=255)
                    tctggtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGGGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCCAATACCCCTCAAGGCTCAcgtagt                                                                                                                               >  2:2281146/1‑145 (MQ=255)
                     ctggtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGACGCGCGGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGtc                                                                                                                              <  2:1485031/150‑2 (MQ=255)
                     ctggtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTa                                                                                                                              <  2:4210831/150‑1 (MQ=255)
                     ctggtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTa                                                                                                                              <  1:3614487/150‑1 (MQ=255)
                     ctggtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTa                                                                                                                              <  1:4673417/150‑1 (MQ=255)
                     ctggtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTa                                                                                                                              <  2:1826125/150‑1 (MQ=255)
                      tggtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAg                                                                                                                             <  2:114813/150‑1 (MQ=255)
                        gtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGAc                                                                                                                           <  1:6795469/150‑1 (MQ=255)
                             gggCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCCTAAAGCCGTCAAGGCTCACTTCGTAGACGAAcc                                                                                                                      >  2:1676436/1‑149 (MQ=255)
                              ggCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCTCGGCGCGGCCAGAAAGCCGTCAAGGCTCACAACGTAGACGAACgg                                                                                                                     >  1:1676857/1‑150 (MQ=255)
                               gCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGGCTCGCGCGGCGCGGCCCGATAGCCCGCAACGCGCACATCGTAGAAGAACcgg                                                                                                                    >  1:6614619/1‑150 (MQ=255)
                                 aaaGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGtt                                                                                                                  <  2:5556728/150‑1 (MQ=255)
                                 aaaGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGtt                                                                                                                  <  1:5479555/150‑1 (MQ=255)
                                 aaaGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGtt                                                                                                                  <  1:2959643/150‑1 (MQ=255)
                                  aaGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCCGATAGCCCGCAACGCGCACAGAGAAGACGAACAGGGTg                                                                                                                 >  1:5162021/1‑150 (MQ=255)
                                    gCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGcc                                                                                                               <  2:4745058/150‑1 (MQ=255)
                                    gCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGcc                                                                                                               <  2:3822170/150‑1 (MQ=255)
                                         tCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCCGATAGCCCGCAAGGCGCACTTCGTAGACGAACGGGTTGcccgcgt                                                                                                          >  2:3252207/1‑146 (MQ=255)
                                         tCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCTCGGCGCGGCCCGATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGGTGCCctcat                                                                                                          >  2:6855421/1‑148 (MQ=255)
                                          cAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCCGCAACGCTCACGTCGTAGACGAAAGGGTTGcccgcatc                                                                                                         >  2:928465/1‑145 (MQ=255)
                                              tCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCcatc                                                                                                     <  1:3807353/150‑1 (MQ=255)
                                                 cctgcatGACGGCAGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCCCTCGGCGCGGCCAAATAGCCGTCAAGGCGCAAATCGTAGAAGAACGGGTTGCCCTCATACatcatc                                                                                                  >  2:618939/7‑150 (MQ=255)
                                                  aCTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCAtcatca                                                                                                 <  2:5961310/150‑1 (MQ=255)
                                                   cTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGGGCCGCGCGCGGCGCGGCCGGCTAGCCGTCAAGGCTCCCGTCGTAGACGCACGGGGTGCCCGCGTCCATCAGCAc                                                                                                >  1:344070/1‑150 (MQ=255)
                                                    tCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGGGGCGCGCGCCGCGCGGCCCGAAAGCCGGCAAGGCGCACTTCGTAGACGAACAGGATGCCCACGTACAacatcaac                                                                                               >  1:864193/1‑148 (MQ=255)
                                                    tCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCGCGGCGCGGCCCAATAGCCGTCAAGGCTCACATCGTAGACGAACGGGTTGCCCTCATCcatcaaaaac                                                                                               >  2:5909690/1‑145 (MQ=255)
                                                     ccTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCAccc                                                                                              <  2:895809/150‑1 (MQ=255)
                                                      cTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCCTATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCg                                                                                             >  2:4567483/1‑150 (MQ=255)
                                                       tGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGc                                                                                            >  2:5097722/1‑150 (MQ=255)
                                                       tGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCTCGGCGCGGCCAAAAATCCGTCAAGGCTCACATAGTAGACGAACCGGTTGCCCACTTCCATCAAAAccccc                                                                                            >  2:3754670/1‑148 (MQ=255)
                                                             ccGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATATACGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTACATCAACACCCGCCAGccc                                                                                      >  2:2958391/1‑150 (MQ=255)
                                                             ccGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGccc                                                                                      <  1:15376/150‑1 (MQ=255)
                                                             ccGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGccc                                                                                      <  1:4717884/150‑1 (MQ=255)
                                                               gTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCAt                                                                                    <  1:2220693/150‑1 (MQ=255)
                                                                 cATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCa                                                                                  <  2:1960358/150‑1 (MQ=255)
                                                                   tGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAgg                                                                                <  2:450871/150‑1 (MQ=255)
                                                                       ggTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTgc                                                                            <  1:4076548/150‑1 (MQ=255)
                                                                       ggTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCGCTTCCCTCATCACCCCCCAGCCCAACAGGGTgc                                                                            >  2:3915808/1‑150 (MQ=255)
                                                                          aGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCTCGGCGCGGCCATATAGCCGTCAAGGCACACTTCGTAGAAGAACGGGTTGCCCTCGTCCATCAACACCCGCAAGCCCAGCAGGTTGcgcg                                                                         >  2:1852965/1‑150 (MQ=255)
                                                                              cTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTg                                                                     <  2:6447703/150‑1 (MQ=255)
                                                                              cTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCCGCCAGATAGCCGTCAACGCTCACATCGTAGACGAACGGGTTGCCCGCGTACATCATCACCCGCAAGCCCACCAGGGTGCGCGAcgg                                                                     >  2:5212653/1‑148 (MQ=255)
                                                                                cGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGc                                                                   <  1:2720187/150‑1 (MQ=255)
                                                                                cGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGc                                                                   <  2:4098892/150‑1 (MQ=255)
                                                                                cGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGc                                                                   <  1:2542907/150‑1 (MQ=255)
                                                                                 ggCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCCGCGCGGCCAAATATCCGTCAAGGCTCACTTCGTAGACGAACGGGTTTCCCACATACAACAACACCCCCAAGCCCATCAGGTTGCGCGACAGGcc                                                                  >  2:671865/1‑150 (MQ=255)
                                                                                       gTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCCAATAGCCGTCAAGGCTCACATCGTAGACGAACGGGTTGCCCACTTACATCATCACCCCCAAGCCCAACAGGTTGCGCGACTGGCCAAcgcg                                                            >  2:5339430/1‑150 (MQ=255)
                                                                                       gTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTAGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAAcgcg                                                            >  2:5069550/1‑150 (MQ=255)
                                                                                        tAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCgg                                                           <  2:5405186/150‑1 (MQ=255)
                                                                                        tAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCgg                                                           <  1:6409088/150‑1 (MQ=255)
                                                                                          gAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCCTATAGCCGTCAAGGCTCACTTCGTAGAAGAACCGGTTGCCCGCTTCCAACATCACCCGCCAGCCCCACAGGGTGCGCGACTGGCCAACGCGGGt                                                         >  2:4869760/1‑150 (MQ=255)
                                                                                                cTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCAc                                                   <  2:427321/150‑1 (MQ=255)
                                                                                                 tGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATATCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTTCGCGACAGGCCAACGCGGGTTTCCAcc                                                  >  2:4431902/1‑150 (MQ=255)
                                                                                                   gcgcAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTc                                                <  2:5714005/150‑1 (MQ=255)
                                                                                                     gcAGTTCAGGCTCACCCTCGACCGGCGCCTCGCGCGGCGCGGCCAGATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCGTCAATCATCACCCGCAACCCCAGCAGGTTGCGCGACAGGCCAACGCGGGTTTCCACCTCGa                                              >  2:2612567/1‑150 (MQ=255)
                                                                                                           cAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCAAATAGCCGTCAAGGCTCACATCGTAGACGAACGGGTTGCCCTCTTCCAACATCACCCCCAAGCCCAGAAGGGTGCGCGACTGGCCCACGCGGGTGTCCACCGCGAAGCCCt                                        >  2:4749314/1‑150 (MQ=255)
                                                                                                            aGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATATCCCTCAAGGCTCACTTCGTAGACGAACGGGTTGGCCGCTTCCCTCATCACCCGCAAGCCCAGCAGGTTGCGCGACGGGGCAACCCGGGTTTCCACCGCGAAGcccag                                       >  2:5935860/1‑148 (MQ=255)
                                                                                                             ggCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGc                                      <  2:4608957/150‑1 (MQ=255)
                                                                                                              gCTCACCCTCGACCGGCGCCACCCTCGGCGCCGCCATATAGCCGTCAAGGCTCACATCGTAGACGAAAGGGTTGCCCTCTACCAACATAAACCGCAATCCCATCAGCTTGCGCGACTGGCCAACGCGGGTTTCCACCACGAAGCCCAGCg                                     >  2:3210443/1‑150 (MQ=255)
                                                                                                                 cACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCt                                  <  1:1965493/150‑1 (MQ=255)
                                                                                                                  aCCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTg                                 <  2:2462663/150‑1 (MQ=255)
                                                                                                                   cccTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGc                                <  1:2862827/150‑1 (MQ=255)
                                                                                                                     cTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAg                              <  2:5462340/150‑1 (MQ=255)
                                                                                                                         aCCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCtgt                          <  1:2494419/150‑1 (MQ=255)
                                                                                                                           cGGCGCCTCGCTCGGCGCGGCCATATATCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGc                        >  2:5204598/1‑150 (MQ=255)
                                                                                                                            ggCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCa                       <  2:1278381/150‑1 (MQ=255)
                                                                                                                               gcCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCg                    <  2:4771865/150‑1 (MQ=255)
                                                                                                                               gcCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCg                    >  1:1595656/1‑150 (MQ=255)
                                                                                                                               gcCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTGTCCACCTCGAAGCCCTGCGGCTGCAGCTGTGCAGCg                    >  2:5238553/1‑150 (MQ=255)
                                                                                                                                ccTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCgg                   <  1:3248034/150‑1 (MQ=255)
                                                                                                                                  tcgctcgGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGct                 >  2:6752164/1‑150 (MQ=255)
                                                                                                                                   cgctcgGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGctt                <  1:5367604/150‑1 (MQ=255)
                                                                                                                                    gctcgGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCACCGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGcttc               <  2:6494350/150‑1 (MQ=255)
                                                                                                                                     ctcgGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGcttct              >  2:4802086/1‑150 (MQ=255)
                                                                                                                                     ctcgGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGcttct              >  2:1573105/1‑150 (MQ=255)
                                                                                                                                     ctcgGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCA‑CACGAAGCCCTGCGTCTGCAGCTGTGCAGCGGcttctt             <  2:1080177/150‑1 (MQ=255)
                                                                                                                                      tcgGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGcttctt             >  2:3344703/1‑150 (MQ=255)
                                                                                                                                      tcgGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGcttctt             <  1:4188717/150‑1 (MQ=255)
                                                                                                                                       cgGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGCTTCttt            <  1:6332788/150‑1 (MQ=255)
                                                                                                                                       cgGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGCTTCttt            <  1:1377655/150‑1 (MQ=255)
                                                                                                                                       cgGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGCTTCttt            <  2:3378788/150‑1 (MQ=255)
                                                                                                                                        ggCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGCTTCTTTc           <  1:2672979/150‑1 (MQ=255)
                                                                                                                                        ggCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGCTTCTTTc           <  2:2111190/150‑1 (MQ=255)
                                                                                                                                        ggCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGCTTCTTTc           <  2:2752741/150‑1 (MQ=255)
                                                                                                                                          cgcgGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGCTTCTTTCaa         >  1:6320703/1‑150 (MQ=255)
                                                                                                                                           gcgGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGCTTCTTTCAAc        <  1:5615144/150‑1 (MQ=255)
                                                                                                                                              gCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGCTTCTTTCAACGcc     <  1:4308791/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ATCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTGCAGCTGTGCAGCGGCTTCTTTCAACGCCGGG  >  NC_002947/5776009‑5776303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑