Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,875,298 G→C 7.1% intergenic (+54/+80) PP_5144 → / ← ptsP hypothetical protein/phosphoenolpyruvate‑dependent regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,875,2980GC 7.1% 597.1 / 4.9 194intergenic (+54/+80)PP_5144/ptsPhypothetical protein/phosphoenolpyruvate‑dependent regulator
Reads supporting (aligned to +/- strand):  ref base G (94/82);  new base C (16/2);  total (110/84)
Fisher's exact test for biased strand distribution p-value = 4.74e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

CTGATTGTGGATGATCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGCA  >  NC_002947/5875152‑5875445
                                                                                                                                                  |                                                                                                                                                   
  gATTGTGGATGATCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTg                                                                                                                                                <  2:4116668/150‑1 (MQ=255)
  gATTGTGGATGATCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTg                                                                                                                                                >  2:3950631/1‑150 (MQ=255)
  gATTGTGGATGATCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTg                                                                                                                                                <  2:3445050/150‑1 (MQ=255)
   aTTGTGGATGATCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTgg                                                                                                                                               <  2:1299209/150‑1 (MQ=255)
   aTTGTGGATGATCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTgg                                                                                                                                               <  2:6701556/150‑1 (MQ=255)
   aTTGTGGATGATCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTgg                                                                                                                                               <  1:3840996/150‑1 (MQ=255)
     tgtgGATGATCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGt                                                                                                                                             <  2:5147734/150‑1 (MQ=255)
     tgtgGATGATCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGt                                                                                                                                             >  2:6603320/1‑150 (MQ=255)
         gatgatCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCaa                                                                                                                                         <  1:1246305/150‑1 (MQ=255)
          atgatCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCCTTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAAACGCCTGGGTCCAAc                                                                                                                                        >  2:3697829/1‑150 (MQ=255)
            gatCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCGCCACCAACCGCCAGGGTCCCAccc                                                                                                                                      >  1:6261269/1‑150 (MQ=255)
              tCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCa                                                                                                                                    <  1:5872267/150‑1 (MQ=255)
               cAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCGGGGTCCAACCCCAg                                                                                                                                   >  2:2991343/1‑150 (MQ=255)
                aGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGa                                                                                                                                  <  2:4796816/150‑1 (MQ=255)
                aGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGa                                                                                                                                  <  1:5533583/150‑1 (MQ=255)
                aGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGa                                                                                                                                  <  1:1502718/150‑1 (MQ=255)
                aGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGa                                                                                                                                  <  2:1880328/150‑1 (MQ=255)
                aGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCGCCTACAACGGCCTGGGTCCAACCCCAGa                                                                                                                                  >  2:497331/1‑150 (MQ=255)
                   ggCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGAtgt                                                                                                                               <  2:2851818/150‑1 (MQ=255)
                            ggcTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGTAACCCCGCCTACAAAGGCCTGGGTCCAACCCCAGATGTGTGTaaggg                                                                                                                      >  1:1092184/1‑146 (MQ=255)
                            ggcTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGAtgt                                                                                                                               >  1:6568443/1‑141 (MQ=255)
                            ggcTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTg                                                                                                                      <  2:5545019/150‑1 (MQ=255)
                             gCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGg                                                                                                                     >  1:404900‑M2/1‑149 (MQ=255)
                              cTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgt                                                                                                                    >  1:1463598‑M2/1‑148 (MQ=255)
                               tGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgta                                                                                                                   >  1:682027‑M2/1‑147 (MQ=255)
                               tGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgta                                                                                                                   >  2:2019046‑M2/1‑147 (MQ=255)
                               tGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgta                                                                                                                   >  2:6486480‑M2/1‑147 (MQ=255)
                               tGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGGGAAACCCCCCCAACAACCGCCCGGGGCCAACCCCCCAAGTGTGTACggggga                                                                                                                   >  1:1961427/1‑148 (MQ=255)
                                ggTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtag                                                                                                                  <  2:6814893‑M2/150‑5 (MQ=255)
                                 gTTGAGCACAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGGGGAACCGCTCCTAAAACGGCCTGGGTCCAAACCCAGATGTGTGTAAGTGgtagg                                                                                                                 <  2:2920012‑M2/150‑6 (MQ=255)
                                    gAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagc                                                                                                              >  2:6137831‑M2/1‑142 (MQ=255)
                                     aGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcg                                                                                                             >  1:6008811‑M2/1‑141 (MQ=255)
                                     aGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcg                                                                                                             >  1:449312‑M2/1‑141 (MQ=255)
                                      gcgcAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgg                                                                                                            >  1:4834069‑M2/1‑140 (MQ=255)
                                      gcgcAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGGGGACCCGCGCCCACACCCGCCGGGGGGCCCCCCCCCCGGTGTGTAGGGGGTGGGAGCgg                                                                                                            >  1:845733/1‑150 (MQ=255)
                                       cgcAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcggg                                                                                                           >  1:5388928‑M2/1‑139 (MQ=255)
                                       cgcAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcggg                                                                                                           >  2:3075247‑M2/1‑139 (MQ=255)
                                       cgcAGTTTCGGGCCTGTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCGTCGCGGGTGAACCCGCTCCTACCACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcggg                                                                                                           >  2:5726023‑M2/1‑139 (MQ=255)
                                        gcAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggt                                                                                                          >  1:5614159‑M2/1‑138 (MQ=255)
                                        gcAGGTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggt                                                                                                          >  2:6199769‑M2/1‑138 (MQ=255)
                                          aGTTTCGGGCCTGTGGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttc                                                                                                        >  2:3926305‑M2/1‑136 (MQ=255)
                                           gTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttca                                                                                                       >  1:3423319‑M2/1‑135 (MQ=255)
                                              tCGGGCCTTTTGGCGGGCATTTGGGTGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcaccc                                                                                                    >  2:5701763‑M2/1‑132 (MQ=255)
                                              tCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcaccc                                                                                                    >  1:1268258‑M2/1‑132 (MQ=255)
                                              tCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcaccc                                                                                                    >  1:1904958‑M2/1‑132 (MQ=255)
                                                gggCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCAACGACAAAGGGCGGGGTACAACCCCCGATGTGTTTACTTGgtaggagcggggtaaccccc                                                                                                  <  1:2799024‑M2/150‑21 (MQ=255)
                                                gggCCTTTTGGAGGGCAGTTGGTGTAAATTGATTTGTTGGTATGATTATTTGATGTTGTCTGTTCGGGCATCTTCGCGGGTGTAACCCCTCCAAAAAACGCATTGGTCCAACCCCAGATGTGGTTTAGTGGTAGGAGCGGGttaaacccc                                                                                                  >  2:769965/1‑143 (MQ=255)
                                                  gCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTGGGGCCTCTTCGCGGGTGAACCCGCTCCTACCCCGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggtgaacccgctc                                                                                                >  2:3493702‑M2/1‑128 (MQ=255)
                                                   ccTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACACCGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaa                                                                                               >  2:5086063‑M2/1‑127 (MQ=255)
                                                     ttttGGCGGGCATTTGGGTTAAATTGAGTTGTTGGTCTTATTATTTTATGTTTTCTGTTCGGGCCTCTTCGCGGTTGAACCCGATCCTACAACGGCCTGGGTCCAACCCCATATGTGTGTACGTGgtaggagcgggttcacccgcgaaga                                                                                             >  2:4515915‑M2/1‑125 (MQ=255)
                                                     ttttGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCCCTCCAACAACCGCCGGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcaaccccgaaga                                                                                             <  2:3142582‑M2/150‑26 (MQ=255)
                                                     ttttGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCCCACCAACAACCGCCGGGGTCCAAACCCAGATGTTTGTACGGGgtgggagcggggtaacccccgaaga                                                                                             <  2:266447‑M2/150‑26 (MQ=255)
                                                           cGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCAGGGTCCAAACCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCgg                                                                                       >  2:1616006/1‑150 (MQ=255)
                                                            gggCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGGGGTAGGAGCGGGTTCACCCCCGAAGAGGCCGGc                                                                                      >  2:3143895/1‑150 (MQ=255)
                                                            gggCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAAACCGCTCCTACAACCGCCAGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCAACCCCGAAGAGGCCGGc                                                                                      >  2:1160969/1‑150 (MQ=255)
                                                              gCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGGGTACCCCCACCAACAAACGCCAGGGTACAAACCCCCAAGATTGTACGGTGTTGGAGCGGGGTGACCCCCCAACAGGCCCGccc                                                                                    >  1:2934164/1‑148 (MQ=255)
                                                                 tttGGGGTAATTTGCGTTGTTGGTCTTTGTTTTTGTTGTTTTCTTTTGGGGCCTCTTGGGGGGTGAACCCGCTCAAACACCGGCCGGGGTCCAACCCCTTTTGTGTGTAGGTGTTCGTCGGGGTTTCACCCGCGAAGAGGCCGGCACaaa                                                                                 <  2:3312236/150‑1 (MQ=255)
                                                                 tttGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCGCCTACAAAGGCCAGGGTCCAACCCCAGATGTTTGTAAGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACaaa                                                                                 >  1:6189590/1‑150 (MQ=39)
                                                                     ggggAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcaccc                                                                                                    <  2:3552044‑M2/127‑19 (MQ=255)
                                                                     ggggAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCAGGGTCCAAACCCACAAGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGc                                                                             >  2:5250306/1‑150 (MQ=255)
                                                                      gggAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCa                                                                            >  2:6284158/1‑150 (MQ=255)
                                                                                 ttgttgGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTGCAACGGCCTGGGTCCAACCCCAGATGTGTGGAGGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTcaca                                                                 >  2:2778652/1‑150 (MQ=255)
                                                                                       gTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAAAGGCCTGGGTCCAACCCCCGATGTGGGTACGGGGTTGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCAACACCCTCACAgggggt                                                           >  2:5242407/1‑147 (MQ=38)
                                                                                         cTGATTTTTTGATGTTGGCTGTTCGGGCCTCTCCGGGGTTGAACCCGCCCCTACACCGGCCTGGGCCCACCCCCAGATGTGTGTACGTGGTCGGCGGGGGTACCCCCGCGAAGAGCCCGGCACAAACAGCATCACCCTCACAGGGTTtta                                                         <  1:1622959/150‑1 (MQ=255)
                                                                                          tGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTGttat                                                        >  2:4456895/1‑150 (MQ=255)
                                                                                                tttGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCGCCAACAACGGCCGGGGTCCAACCCCAGATGTGTGTACGTGGTAGGGGCCGGTTCAACCGCGAAGAGGCCGGCACAAACAGCAACACCCACACAGGGGTTTTTTAGccc                                                  >  2:2409373/1‑150 (MQ=37)
                                                                                                tttGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAAACCGCGCCAACAACCGCCTGGGTCCAACCCCACATGTGTGTACGTGGTGGGAGCGGGTTCACCCCCCAAGAGGCCGGCACAAACAGCATCACCCACACAGGGTTTTATTAGccc                                                  >  2:1946154/1‑150 (MQ=255)
                                                                                                 tttaTGTTGTCTGTTCGGGCCCATTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCATATGTGTGTACGTGGTAGGCGCGGTTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGcccc                                                 <  2:1077896/147‑1 (MQ=38)
                                                                                                       ttgtCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCAGGGTCCCACCCCAGATGTGTGTACGTGGTAGGGGCGGGGTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGccc                                           >  2:2145008/1‑150 (MQ=255)
                                                                                                            tGTTCGGGCCTCTTCGCGGGTGTAACCCCGACTACAAACGCCGGGGTCCAAACCCACATTTGTGTACGTGGTAGGTGCGGGTTCACCCCCGAAGAGGCCCGCAAAAAAAGCAACACCCAAACAGGGTTTTATTATCACCAGGCCCaaaaa                                      >  2:228619/1‑147 (MQ=255)
                                                                                                                ggggCCTCTGGGGGGGTTACCCGGCTCATACCAGGGCGTGGGTCCACCCCCTTTGGTGTGTCGGGGTTTGTTGCGGGTACCCCCGCTAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCcg                                  <  2:833030/149‑1 (MQ=255)
                                                                                                                     ctctTCGCGGGTGAACCCGCTCCGACAACGGCCGGGGTCCCACCCCACATGTGGGTACCGGGTAGGAGCGGGGTCACCCGCGAAGCGGCCGGCACAAACAGCATCACCCACACAGGGTTTTATTAGCCCCCGGCCCCAACAGCCGCGCCa                             >  2:5888724/1‑150 (MQ=255)
                                                                                                                      tctcCGGGGGTCAACCCGCTCCTACAAGGGCCTGGGTCAAACCCCTGTTGTTTTTAGGGGGTCGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCaa                            <  2:3779474/150‑1 (MQ=255)
                                                                                                                            gggttgcaCCCGACCCAACACCGGCGGGGGTCCAACCCATTTTGTGTGTAGGGGGTCGGTGGGGGTACACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAg                      <  1:6205684/143‑1 (MQ=255)
                                                                                                                             gggTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaagaggcccgaacagacaacatcaaatactcagaccaacaactcaatttcccccaaatgcccgccaaaaggcccga                     <  1:5190281‑M2/150‑98 (MQ=255)
                                                                                                                                   aCCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaagaggcccgaacagacaacatcaaatactcagaccaacaactcaatttcccccaaatgcccgccaaaaggcccgaaactgc               <  2:4394893‑M2/150‑104 (MQ=255)
                                                                                                                                   aCCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGttcttc               >  2:6228371/1‑150 (MQ=255)
                                                                                                                                    cccGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaagaggcccgaacagacaacatcaaatactcagaccaacaactcaatttcccccaaatgcccgccaaaaggcccgaaact                 >  2:886474‑M2/1‑46 (MQ=255)
                                                                                                                                    cccGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCa              >  2:6760298/1‑150 (MQ=255)
                                                                                                                                     ccGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaagaggccgaacagacaacatcaaatactcagaccaacaactcaatttcccccaaatgcccgccaaaaggcccgaaactgcgct             <  2:6109200‑M2/150‑106 (MQ=255)
                                                                                                                                     ccGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAgg                     <  2:5601607/142‑1 (MQ=255)
                                                                                                                                      cGCTCCTACACCGGCCTGGGTCAAACCCATTTTGTGTTTAGGTTTTAGTAGGGGTTACACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAgg            <  2:696472/150‑1 (MQ=255)
                                                                                                                                       gCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaagaggccggcacaaacagcatcaaatactcagaccaacaactcaatttcccccaaatgcccgccaaaaggcccgaaactgcgctc           <  2:2496926‑M2/150‑108 (MQ=255)
                                                                                                                                         tCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaagaggcccgaacagacaacatcaaatactcagaccaacaactcaatttcccccaaatgcccgccaaaaggcccgaaactgcgctcaa         <  1:4419347‑M2/150‑110 (MQ=255)
                                                                                                                                         tCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGcc         >  1:4546572/1‑150 (MQ=255)
                                                                                                                                         tCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGcc         >  2:2721942/1‑150 (MQ=255)
                                                                                                                                          ccTACACCGGCCGGGGCCCAACCCCAGTTGTGGGTACGTGGTCGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCa        <  2:5423155/150‑1 (MQ=255)
                                                                                                                                           cTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAg       >  2:1105964/1‑150 (MQ=255)
                                                                                                                                           cTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAg       >  1:5417366/1‑150 (MQ=255)
                                                                                                                                            tACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGccc                                           <  1:6608790/113‑1 (MQ=255)
                                                                                                                                             ccaccGGCCTGGGTCAAACCCCTGATGTGTGTACGTGGTCGGAGCGGGTTCCCCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGtt     <  2:3263602/146‑1 (MQ=255)
                                                                                                                                               aaCGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaagaggcccgaacagacaacatcaaatactcagaccaacaactcaatttcccccaaatgcccgccaaaaggcccgaaactgcgctcaaccagcc   <  1:747783‑M2/150‑116 (MQ=255)
                                                                                                                                               aaCGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCg                                                                                        >  1:3032101/1‑65 (MQ=255)
                                                                                                                                               aaCGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCg                                                                                        <  2:3032101/65‑1 (MQ=255)
                                                                                                                                               aaCGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGc   >  1:4552658/1‑150 (MQ=255)
                                                                                                                                               aaCGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGc   >  1:4730952/1‑150 (MQ=255)
                                                                                                                                               aaCGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGc   >  1:4552533/1‑150 (MQ=255)
                                                                                                                                               aaCGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGc   >  2:2640653/1‑150 (MQ=255)
                                                                                                                                               aaCGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGc   >  2:5373655/1‑150 (MQ=255)
                                                                                                                                                ccGGCCTGGGCCCAACCCAATATGTGTGTACGTGGTAGGAGGGGGTCCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGCa  <  2:4220461/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTGATTGTGGATGATCAGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGCA  >  NC_002947/5875152‑5875445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑