BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,973,424	C→G	6.5%	T51T (ACG→ACC)	PP_5723 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,973,424	0	C	G	6.5%	480.6 / 4.5	150	T51T (ACG→ACC)	PP_5723	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (18/121); new base G (8/3); total (26/124)
Fisher's exact test for biased strand distribution p-value = 3.42e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

AAAAACAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGG  >  NC_002947/5973275‑5973572

aaaaaCAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGc                                                                                                                                                        <  1:1821336/150‑1 (MQ=255)

  aaaCAGCGCGTTATGTTATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGt                                                                                                                                                      <  1:2742340/150‑1 (MQ=255)

  aaaCAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGt                                                                                                                                                      <  1:3617851/150‑1 (MQ=255)

        cgcgTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGTGGCCTTGCGTCG‑CGAt                                                                                                                                               <  2:5900190/150‑1 (MQ=255)

         gcgTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGAtt                                                                                                                                              <  2:3790608/150‑1 (MQ=255)

         gcgTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTGGCGTCG‑CGAtg                                                                                                                                              >  2:3878893/1‑149 (MQ=255)

          cgTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTg                                                                                                                                             <  2:3368033/150‑1 (MQ=255)

          cgTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTg                                                                                                                                             <  1:5276986/150‑1 (MQ=255)

          cgTGATGTGATGTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTg                                                                                                                                             <  2:2402420/150‑1 (MQ=255)

            ttATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTggg                                                                                                                                           <  2:240955/150‑1 (MQ=255)

            ttATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTggg                                                                                                                                           <  2:5148554/150‑1 (MQ=255)

            ttATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTggg                                                                                                                                           <  1:3361671/150‑1 (MQ=255)

            ttATGTGATGTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCTCAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTggg                                                                                                                                           <  1:3828785/150‑1 (MQ=255)

            gtATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTggg                                                                                                                                           <  1:3146278/149‑1 (MQ=255)

              aTGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGt                                                                                                                                         <  1:5640200/150‑1 (MQ=255)

              aTGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGCG‑CGAGTggggg                                                                                                                                         >  1:6270969/1‑149 (MQ=255)

              aTGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCGTGGGGCG‑CGGGTggggg                                                                                                                                         >  1:6270912/1‑149 (MQ=255)

                gtgATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGc                                                                                                                                       <  1:4894357/150‑1 (MQ=255)

                gtgATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGc                                                                                                                                       <  1:974355/150‑1 (MQ=255)

                   aTTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGaa                                                                                                                                    <  1:2208637/150‑1 (MQ=255)

                    tttGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCATGCGGCG‑CGCGTGGGGGGCGCAg                                                                                                                                   >  2:2220137/1‑150 (MQ=255)

                      tGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAgcg                                                                                                                                 <  2:5251915/150‑1 (MQ=255)

                         tCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑ccc                                                                                                                             <  1:4801482/150‑1 (MQ=255)

                              aTTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCaa                                                                                                                        <  1:4451552/150‑1 (MQ=255)

                               ttGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAg                                                                                                                       <  2:74736/150‑1 (MQ=255)

                                  gCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCGTGGGGCG‑CGAATGGGGTGGGCAGCG‑gcccccccgagg                                                                                                                    >  2:3510317/1‑144 (MQ=255)

                                   ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGAttt                                                                                                                   <  2:5499276/150‑1 (MQ=255)

                                   ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGAttt                                                                                                                   <  2:1603549/150‑1 (MQ=255)

                                   ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGAttt                                                                                                                   <  2:1294040/150‑1 (MQ=255)

                                   ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGCG‑GGATTGGGGTGGGGAGCG‑cccccccacagtt                                                                                                                   >  2:6407625/1‑143 (MQ=255)

                                    cAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTtg                                                                                                                  <  1:3190784/150‑1 (MQ=255)

                                     aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTtgt                                                                                                                 <  2:3457782/150‑1 (MQ=255)

                                     aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTtgt                                                                                                                 <  2:558487/150‑1 (MQ=255)

                                     aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTtgt                                                                                                                 <  1:6638368/150‑1 (MQ=255)

                                      gTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACATCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTtgtg                                                                                                                <  1:2181138/150‑1 (MQ=255)

                                       tCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGc                                                                                                               <  1:196108/150‑1 (MQ=255)

                                       tCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGc                                                                                                               <  1:237224/150‑1 (MQ=255)

                                       tCGCCAGCACGGGAAACATGCAAAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCATTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGc                                                                                                               <  1:237237/150‑1 (MQ=255)

                                        cGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGcc                                                                                                              <  1:5579033/150‑1 (MQ=255)

                                            aGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGa                                                                                                          <  2:4142369/150‑1 (MQ=255)

                                               aCGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGc                                                                                                       <  1:5191591/150‑1 (MQ=255)

                                               aCGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGc                                                                                                       <  2:3093791/150‑1 (MQ=255)

                                                cGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCt                                                                                                      <  2:2734632/150‑1 (MQ=255)

                                                 gtgAAACATTCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTg                                                                                                     <  1:1880911/148‑1 (MQ=255)

                                                 gggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTg                                                                                                     >  2:3380716/1‑150 (MQ=255)

                                                 gggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTg                                                                                                     <  1:1880976/150‑1 (MQ=255)

                                                  ggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGGTTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGa                                                                                                    <  2:1942810/150‑1 (MQ=255)

                                                  ggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGGTTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGa                                                                                                    <  2:3714826/150‑1 (MQ=255)

                                                  ggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGa                                                                                                    <  2:4609587/150‑1 (MQ=255)

                                                  ggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGa                                                                                                    <  1:2459237/150‑1 (MQ=255)

                                                  ggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGa                                                                                                    <  1:2459236/150‑1 (MQ=255)

                                                   gAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGGGGCG‑CGATGGGGGTGCGAAGCG‑CCCCCCCAGGTTTGTGCGGTGATGCTGat                                                                                                   >  2:6822654/1‑150 (MQ=255)

                                                   gAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGat                                                                                                   <  1:5825863/150‑1 (MQ=255)

                                                      aCATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATAtt                                                                                                <  2:5790515/150‑1 (MQ=255)

                                                        aTGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCTAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGc                                                                                              <  1:6502832/150‑1 (MQ=255)

                                                         tGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGcc                                                                                             <  2:2828728/150‑1 (MQ=255)

                                                           cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCgg                                                                                           <  1:2899495/150‑1 (MQ=255)

                                                           cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGCG‑CGAATGGGGTGCGAAGCG‑CCCCCCCAGAATTTTTCCGTTATGAAGATAtttacgg                                                                                           >  2:3308799/1‑145 (MQ=37)

                                                             caATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCgggg                                                                                         <  1:5249161/150‑1 (MQ=255)

                                                             caATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCgggg                                                                                         <  1:5915026/150‑1 (MQ=255)

                                                              aaTTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGc                                                                                        <  1:1762052/150‑1 (MQ=255)

                                                                  tCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCt                                                                                    <  2:941999/150‑1 (MQ=255)

                                                                   cGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCtg                                                                                   <  1:1058996/150‑1 (MQ=255)

                                                                    ggTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCtgt                                                                                  <  2:2906252/150‑1 (MQ=255)

                                                                    ggTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCtgt                                                                                  <  2:4630659/150‑1 (MQ=255)

                                                                    ggTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCtgt                                                                                  <  1:5890683/150‑1 (MQ=255)

                                                                     gTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCtgtg                                                                                 <  2:5881008/150‑1 (MQ=255)

                                                                     gTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCtgtg                                                                                 <  1:5553942/150‑1 (MQ=255)

                                                                      tGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGc                                                                                <  1:299662/150‑1 (MQ=255)

                                                                       gcgcAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCa                                                                               <  1:1439642/150‑1 (MQ=255)

                                                                       gcgcAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCa                                                                               <  2:1064182/150‑1 (MQ=255)

                                                                        cgcAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAg                                                                              <  1:1356352/150‑1 (MQ=255)

                                                                        cgcAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAg                                                                              <  1:1356389/150‑1 (MQ=255)

                                                                        cgcAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAg                                                                              <  2:5823341/150‑1 (MQ=255)

                                                                         gcagcaGCGGGGCATGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTGGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATGTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGc                                                                             <  1:7404/150‑1 (MQ=255)

                                                                         gcagcaGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGc                                                                             <  1:5361401/150‑1 (MQ=255)

                                                                         gcagcaGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGc                                                                             <  1:1569928/150‑1 (MQ=255)

                                                                         gcagcaGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGc                                                                             <  1:4005607/150‑1 (MQ=255)

                                                                         gcagcaGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGc                                                                             <  1:3317605/150‑1 (MQ=255)

                                                                           agcagcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGccc                                                                           <  1:6524066/150‑1 (MQ=255)

                                                                              agcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAAt                                                                        <  1:4506750/150‑1 (MQ=255)

                                                                               gcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAATc                                                                       <  1:3380716/150‑1 (MQ=255)

                                                                               gcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAATc                                                                       <  2:6286381/150‑1 (MQ=255)

                                                                                   ggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGCTTGGGGGGCGAAGCG‑CCCCCCAAGATTTGTGCCGTGGTGCGGTTATTGGCGGGGCCGCTGTGCAGCCCAATCGCGa                                                                   >  2:5645251/1‑150 (MQ=255)

                                                                                   ggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAATCGCGa                                                                   <  2:54155/150‑1 (MQ=255)

                                                                                   ggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAATCGCGa                                                                   <  2:5520929/150‑1 (MQ=255)

                                                                                   ggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAATCGCGa                                                                   <  2:5520515/150‑1 (MQ=255)

                                                                                    gCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAATCGCGAc                                                                  <  1:332939/150‑1 (MQ=255)

                                                                                    gCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAATCGCGAc                                                                  <  2:6240753/150‑1 (MQ=255)

                                                                                    gCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAATCGCGAc                                                                  <  2:3626562/150‑1 (MQ=255)

                                                                                    gCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAATCGCGAc                                                                  <  2:3626561/150‑1 (MQ=255)

                                                                                         cTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAg                                                             <  1:399548/150‑1 (MQ=255)

                                                                                                     tgtgCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGata                                                                                                  >  2:199604/1‑101 (MQ=255)

                                                                                                          aaCAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCGTGGGGCG‑CGGGTGGGGGGCGGAGCGCCCCCCCAAAAATTTTG‑CGTGGGGCTGATATTTACCGGGCCCCGGGGCAGCCCCATACCCACGCCACGCCCCCCCCCAAGGccc                                            >  2:3310633/1‑149 (MQ=255)

                                                                                                           aCAGCTTTAAAAGGGCCCGCGTTCTTTGGGGGCGCGGCCTTGCGTCG‑CGATTGGGGGGCGACGCG‑CCCCCCAATTTTTGTGCTTTGAGGCTTTTTTTGCGGGGGCGGGTGTGCAGCCCAACCGCGACGCAAGGCCGCACACAAAGGCCaa                                           <  2:6783171/150‑1 (MQ=255)

                                                                                                            cAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑ccccc                                                                                                                           >  1:1983190/1‑69 (MQ=255)

                                                                                                                                      gtgGGAGCGGCCTTGGGTCG‑CGTTGGGGGTGCTAGGCG‑CCCCCCATGTTTTGTGCGTTGTTGCTTATTGGGCGGGGGCCGCTGGGCAGCCCACGCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTgtgcg                <  2:6011226/150‑1 (MQ=255)

                                                                                                                                           aGCGGCCTTGCGTGGCCCATTGGGG‑GCGAAGAC‑CCCCCCCAGATTTTTGCCGTGGTTCTGATATATCCCGGGCCGCAGTGCAGCCCAAAAGCCAACCAAGGCCGCACACAAAGGCCAAAGTGCCCGCAGGCGCCAGGGTTGTGCGGGCTa           >  2:659223/1‑150 (MQ=255)

                                                                                                                                                ccTTGCGTCG‑CGATTGGGGGGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATAGTGCCGGGGCAGCTGTGCAGCCCAAACGCCACGCAAGCACGCACACAAACGCCAATGTTCACACAGGCGCCAGGGTTGTTCGTGCTACACTg      >  1:3054187/1‑150 (MQ=255)

                                                                                                                                                    gCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggcgg  >  1:1040343/1‑150 (MQ=255)

AAAAACAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCG‑CGATTGGGGTGCGAAGCG‑CCCCCCAAGATTTGTGCCTTGATGCTGATATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGG  >  NC_002947/5973275‑5973572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`