BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	524,178	A→C	7.5%	intergenic (+42/‑774)	tyrS → / → PP_16SC	tyrosine‑‑tRNA ligase/16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	524,178	0	A	C	7.5%	351.9 / 10.0	157	intergenic (+42/‑774)	tyrS/PP_16SC	tyrosine‑‑tRNA ligase/16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base A (31/94); new base C (5/9); total (46/111)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

GTTGATGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTC  >  NC_002947/524029‑524327

gTTGATGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTa                                                                                                                                                         <  1:5424879/150‑1 (MQ=255)

 ttGATGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAg                                                                                                                                                        <  1:3292163/150‑1 (MQ=255)

 ttGATGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAg                                                                                                                                                        <  2:5380292/150‑1 (MQ=255)

   gATGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGc                                                                                                                                                      <  2:118455/150‑1 (MQ=255)

   gATGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGc                                                                                                                                                      <  1:5078546/150‑1 (MQ=255)

   gATGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGc                                                                                                                                                      <  1:5382048/150‑1 (MQ=255)

     tGGTGCAGTGGTCGACAAGGACTTCTTGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGT‑CCGGTAGGCC‑t                                                                                                                                                   <  1:4182516/150‑1 (MQ=255)

        tGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCTTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑Tccc                                                                                                                                                <  2:342823/150‑1 (MQ=255)

        tGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑Tccc                                                                                                                                                <  2:1295927/150‑1 (MQ=255)

        tGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGGAGGCC‑Accc                                                                                                                                                >  2:2767218/1‑150 (MQ=255)

         gCAGTGGTCGACAAGGACTTCATGTCTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCTACCGGCC‑Tcccc                                                                                                                                               <  1:2057887/150‑1 (MQ=255)

            gTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCTACCGGCC‑TCCCCGC‑t                                                                                                                                           <  2:2902288/150‑1 (MQ=255)

             tGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑tt                                                                                                                                          <  2:3230772/150‑1 (MQ=255)

                  gACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGCTGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCCGGAGGCC‑GCCCCGcgtatttt                                                                                                                                      >  2:6073569/1‑143 (MQ=255)

                     aaGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTg                                                                                                                                  <  2:3197124/150‑1 (MQ=255)

                      aGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTgg                                                                                                                                 <  2:3236843/150‑1 (MQ=255)

                      aGGACTTATTGTTTGTCCTTGGTGCTACCCATGTGTGCAATGCGGGAAATACGTCTTTTGGCCGGGTTACCCTCAAGGCTGTGTGATGGCCTGGTTGGGGTCGCTATATAGACGC‑GGGGCGG‑CCGGTTGGCC‑TCCCCGC‑TTTGTTTTTgg                                                                                                                                 <  1:1174515/150‑1 (MQ=255)

                          cTTCATGTTTGTCCTTGGTGCTACCCATGTGTGTCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCCGGCGGCC‑CCCCCGC‑CGTGTTTTTggtggt                                                                                                                             >  2:2376531/1‑146 (MQ=255)

                           ttCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGGGGG‑CGGGGGGGCC‑CCCCCCC‑AGGGTTTTTGGGGtt                                                                                                                             >  1:3583496/1‑150 (MQ=255)

                           ttCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑CCGGGCGGCC‑ACACCCC‑GGTGTTTTTgggggtg                                                                                                                            >  2:244813/1‑147 (MQ=255)

                           ttCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑CCGGGAGGCCGCCCCCCC‑ATGGTTTTTGGGGtt                                                                                                                             >  1:2704399/1‑150 (MQ=255)

                             cATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTtgg                                                                                                                          <  2:4038455/150‑1 (MQ=255)

                              aTGTTTGTCCTTGGTGCTACCCTTGTGTGCCTAGCGGGCAATATGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTtggt                                                                                                                         <  2:642102/150‑1 (MQ=255)

                               tttttGTCCTTGGTGCTACCATTGTGTGCCATGCGGGAATTAAGTCGTTGGGCCGGGTTACCCTCAAGGCTGCGTGTTTGCGTGGTTGGTGTAGCTATATAGACGG‑GGGGAGG‑CCGGTTGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTtggtg                                                                                                                        <  1:5875065/148‑1 (MQ=255)

                               tGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCACGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGACGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTtggtg                                                                                                                        <  1:6703746/150‑1 (MQ=255)

                               tGTTTGTCCTGGGTGCTACCCTTGTGTGCAACGCGGGCAAGACGTCGTTTGGCCGGGTTACCCTCAAGGCTTAGTGATTGCCTGTTTGGTGTAGATATATAGAAGG‑GGGGAGG‑CCGGTTGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTtggtg                                                                                                                        <  1:5892991/150‑1 (MQ=255)

                                 tttGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGTGG‑CCGGGAGGCC‑ACCCCCC‑GGGGGTTTTGGGGTTttgtggg                                                                                                                      >  1:5666905/1‑149 (MQ=255)

                                 tttGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑GCGGGGGGGC‑ACCCCCC‑CGGTTTTTTGGGGggtggtggt                                                                                                                      >  1:3721456/1‑150 (MQ=255)

                                 tttGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑GCGGGAGGCC‑ACCCCCC‑GGTGTTTTTGGGGTTTtgttgt                                                                                                                      >  2:3459258/1‑150 (MQ=255)

                                   tGTCCTTGTTGCTACCCTTGTGTGCCAAGCGGGCAAGACGTGGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTTGCTATATAGACGG‑GGGGCGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTg                                                                                                                    <  1:4239191/150‑1 (MQ=255)

                                    gTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGc                                                                                                                   <  2:482316/150‑1 (MQ=255)

                                    gTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGc                                                                                                                   <  2:3901959/150‑1 (MQ=255)

                                     tCCTTGGTGCTACCCATGTGTGCCAAGCGGGAAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCt                                                                                                                  <  2:1739971/150‑1 (MQ=255)

                                       cTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑CCCGGAGGCCACCCCCCC‑TTTGTGTTTGGGGTTGGTGGGTTgggt                                                                                                                 >  1:1546418/1‑147 (MQ=255)

                                        ttGGTGATACCCTTGTGGGCAATGGGGTAACTAAGTTGTTGGGCGGGGTGACCCTCATGGCTGTGTTTTTGCGTGTTTGGTGTAGCTATATAGAGGG‑GGGGTGG‑CCGTTCGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCtgtt                                                                                                               <  1:2273297/150‑1 (MQ=12)

                                         tGGTGCTCCCCATGTGTGCCACGGGGGCACGAAGTGGTTTGGCCGGTTTACCCTCAAGGCTTTGTGATTGCCTTGATGGTGTAGCTATATCGACGG‑GGGGAGG‑CGGGTTGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCtgttg                                                                                                              <  1:2043576/150‑1 (MQ=37)

                                         tGGTGCTACCCTTGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCATGGCTGAGTGATTGCCTGGATGGTGTAGATATATAGACGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCtgttg                                                                                                              <  1:2043673/150‑1 (MQ=255)

                                         tGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGTTTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCtgttg                                                                                                              <  2:2457934/150‑1 (MQ=255)

                                         tGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGTTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCtgttg                                                                                                              <  1:3255118/150‑1 (MQ=255)

                                              cTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTaggg                                                                                                         <  2:2015882/150‑1 (MQ=255)

                                                 cccATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTCATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑GCCGGAGGCC‑ACCCCCCGATGGTTTTTGGGGTTT‑GTGCTTTGGGTTGGaggggga                                                                                                      >  2:212990/1‑150 (MQ=255)

                                                        gtgCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑GCGGGGGGGC‑GCCCCCC‑GGGGTGTTTTGGGGGTGGTGGTTGGTGGTGTttgggggggggggg                                                                                               >  1:4284286/1‑148 (MQ=255)

                                                         tgCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGTGTTTTTGCCTGTATGGTGTAGATATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTg                                                                                              <  1:6044035/150‑1 (MQ=38)

                                                               gggggCAAGTAGTCGTTTGGCCGGGTTACCCTCAAGGCTGTGTGATTGCCTGGTTGGTGTTGCTATATTGAAGG‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTga                                                                                        <  1:2252162/148‑1 (MQ=38)

                                                               gCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTga                                                                                        <  2:5025976/150‑1 (MQ=255)

                                                                 gggCATGAAGTGTTTTGGCGGGGTTACCCTCTTGGCTGTTTGTTTGCCTGGTTGTTGTAGCTTTATCTACGG‑GGGGAGG‑CGGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTgaga                                                                                      <  1:6564597/150‑1 (MQ=25)

                                                                 gggCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTgaga                                                                                      <  2:1507760/150‑1 (MQ=255)

                                                                    cAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTTGGGC‑GCACCCC‑CTTGTTTTTTGGGTTTGGGGGGTGGGGTTGTTGGGGGTGGGGGGGGttttttgtggg                                                                                   >  2:3703171/1‑143 (MQ=255)

                                                                    cAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAggg                                                                                   <  2:6652858/150‑1 (MQ=255)

                                                                    cAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGGGTAGCTATAGAGAAGC‑GGGGCGG‑CCCGGAGGCC‑ACCCCGC‑GGGGGGTGTGGGGGGTGGTGGTTGCGGGGGGAGGGGGGGGGGGCGGTGTTGAGAggg                                                                                   >  2:5654321/1‑150 (MQ=255)

                                                                         agTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTg                                                                              <  2:2007104/150‑1 (MQ=255)

                                                                         agTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTg                                                                              <  2:4697043/150‑1 (MQ=255)

                                                                              tttGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGTTGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGt                                                                         <  1:1676558/150‑1 (MQ=255)

                                                                              tttGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGt                                                                         <  2:740645/150‑1 (MQ=255)

                                                                              ttgggCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTTTAGCTATATAGACGG‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGt                                                                         <  1:6094161/147‑1 (MQ=255)

                                                                               ttGGCGGGGTTACCCTCATGGCTGAGTTATTGCCTGGATGGTGTAGCTATATAGACGC‑GGGGAGG‑CCGTTCGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTg                                                                        <  1:183715/150‑1 (MQ=38)

                                                                                gggCGGGTTTACCCTCATGGCTGTGTGTTTGCCTGTTTGTTTTAGCTTTATTGATGG‑GGGGAGG‑CCGTTTGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTga                                                                       <  1:1243334/149‑1 (MQ=21)

                                                                                gggCCGGGTTACCCTCATGGCTGAGTTATTGCGTGGATGGTTTTGTTATATAGAAGG‑GGGGTGG‑CCGGTTGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTga                                                                       <  1:5880289/149‑1 (MQ=37)

                                                                                ggccggggTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTga                                                                       <  2:6019080/145‑1 (MQ=255)

                                                                                 ggCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑GCGGGTGGCC‑TCCCCCC‑GGTGTTTTTTGGGTGTTGGGGGTGGTGTTGTTGGGGGGGGGGGGGGGTTTGTGGTGGGGTTGGGGGTgtg                                                                      >  1:5549764/1‑148 (MQ=255)

                                                                                   ccGGGTTACCCTCCCGGCTGAGTGATTGCCTGGAGGGTGTAGCTATATAGCAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGAt                                                                    <  2:5662407/150‑1 (MQ=255)

                                                                                     gggTTACCCTCATGGCTGAGTTATTGCCTGGATGGTGTAGATATATAGAAGG‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGAtgt                                                                  <  1:5593248/150‑1 (MQ=255)

                                                                                       gTTACCCTCATGGCTGAGTGATTGCCTGGTTGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATgtgt                                                                <  1:5502113/150‑1 (MQ=255)

                                                                                              tCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑CCGGGAGGCC‑GCCCCCC‑GGTGGTGTTGGGGGTGGGGGGGTGGGGTGGGTGGGGGGGGGGGCGGGGTTGGGGGGGTGTTTGGGGGGAGCTGTTgccgcccc                                                         >  2:5466605/1‑148 (MQ=255)

                                                                                                   gCTGAGTGATTGCCTGGATGGTGTAGATATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAAccc                                                    <  1:3086922/150‑1 (MQ=255)

                                                                                                   gCTGAGTGATGGCCTGGATGGTGTAGCTATATAGACGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAAccc                                                    <  2:3576404/150‑1 (MQ=255)

                                                                                                    cTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGCGGCCCCGTAGCCA‑ACCCCCG‑GTGGTTTTTTGGGTTT‑GTTGTTGCGGTTTTTTGGGGAGGGGGCGGTTTTTAGAAGGTGTTTTGGGGGCGATTTTTGCCCCACAAccca                                                   >  2:361769/1‑149 (MQ=255)

                                                                                                       tgttaTTGCCTGGATGGTTTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGc                                                <  1:5149237/146‑1 (MQ=255)

                                                                                                        gTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑GCCGGAGGCC‑TCCCCCC‑CTTGTTTTTTGGGGTTTGTTGTTGGGGTTGTGTGGGGGGGGGGGGGGTTTTTGTGGGGGTTGTCGGTGAGATGTTTACGCCCCAAcccaagca                                               >  2:416188/1‑145 (MQ=255)

                                                                                                        gTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑CCCGGGGGCC‑ACCCCGC‑ATTTTGTTTTGGGTTTGGGGGGTGGGGTTTGAGGGGGAGGGGGCGGTTTTTATAGGGTTTTTGCGGTGGGATGTGTACACCACAACCCATGCa                                               >  2:3851229/1‑150 (MQ=255)

                                                                                                        gTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGCGG‑CCCGTAGGCC‑AACCCCC‑CTTGTTTTTTGGGTTTTGTGGTTGCGGTTGTAGGGGGAGGGGGCGGTTTTTAGAGGGGGTTTGCGGTGAGATTTGTACCCCACAAcccaagca                                               >  2:3851039/1‑145 (MQ=255)

                                                                                                        gTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCa                                               <  2:6200964/150‑1 (MQ=255)

                                                                                                        gTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCa                                               <  2:6531588/150‑1 (MQ=255)

                                                                                                              gCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCCGTAAGCC‑ACCCCCC‑GTTGTTTTTTGGGGTTGGTGGTTGGTGGTGTAGGGGGCGGGGGCGGTTTTGAGAGGGGGTTTGCGGTGCGATGTTTCCCCCACAACCCATACAGccccg                                         >  2:6707552/1‑149 (MQ=255)

                                                                                                                cTGGTTGGTGTAGCTATATAGACGG‑GGGGTGG‑CCGGTCGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCtgt                                       <  1:1741359/150‑1 (MQ=25)

                                                                                                                cTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGTTTGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCtgt                                       <  1:760187/150‑1 (MQ=255)

                                                                                                                  ggATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTga                                     <  2:3613449/150‑1 (MQ=255)

                                                                                                                  ggATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTga                                     <  2:3613798/150‑1 (MQ=255)

                                                                                                                    aTGGTGTAGCTATATAGAAGC‑GGGGGGG‑GCGGTTGGGC‑GACCCCC‑CGTGTTTTTGTGGGTTGGTGGGTTGTGGTGTTGGGGGTGGGGGGGGTTTTGTGAGGGGGGTGGCGGTGGGATGTGTTCCCCCCAACCCAGGCACCGCCGGTggga                                   >  2:2534646/1‑150 (MQ=255)

                                                                                                                    aTGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTgaga                                   <  2:930271/150‑1 (MQ=255)

                                                                                                                          tAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAgc                             <  2:2132889/150‑1 (MQ=255)

                                                                                                                             cTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGcgcc                          <  2:783199/150‑1 (MQ=255)

                                                                                                                                 ataGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGccgccc                      <  2:1658557/150‑1 (MQ=255)

                                                                                                                                 aacaaaGC‑GGGGAGG‑CCTACCGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGccgccc                      >  2:111654/5‑150 (MQ=18)

                                                                                                                                     aaGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGt                  <  2:4277406/150‑1 (MQ=255)

                                                                                                                                       gC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTcc                <  2:3521499/150‑1 (MQ=255)

                                                                                                                                        c‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCg               >  1:5682543/1‑150 (MQ=255)

                                                                                                                                         gggggAGG‑CGGGTCGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCg               <  1:4092217/149‑1 (MQ=255)

                                                                                                                                          ggggAGG‑CCTATCGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCgg              >  2:6460202/1‑150 (MQ=255)

                                                                                                                                           gggCGG‑CCGGTAGGCC‑CCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGc             <  1:150646/150‑1 (MQ=255)

                                                                                                                                           gggAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGc             >  1:4866790/1‑150 (MQ=255)

                                                                                                                                           gggAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGCCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGc             >  1:4866778/1‑150 (MQ=255)

                                                                                                                                             gcgg‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCtt           <  1:5069160/148‑1 (MQ=255)

                                                                                                                                             gAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCtt           <  2:4100124/150‑1 (MQ=255)

                                                                                                                                             gAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCtt           <  2:4297171/150‑1 (MQ=255)

                                                                                                                                              aGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGAGCCGCCCGCGTCCGGCTTg          >  1:6192004/1‑150 (MQ=255)

                                                                                                                                                g‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTgag        >  2:355939/1‑150 (MQ=255)

                                                                                                                                                g‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTgag        >  1:2708205/1‑150 (MQ=255)

                                                                                                                                                    ggTAGGCC‑TCCCCTC‑ATTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACa     <  1:676805/150‑1 (MQ=255)

                                                                                                                                                      tAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACAtt   <  2:5360843/150‑1 (MQ=255)

GTTGATGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGGTAGGCC‑TCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTC  >  NC_002947/524029‑524327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`