Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,011,253 C→T 5.6% R371C (CGC→TGC)  ydcJ → metalloprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,011,2530CT 5.6% 1208.8 / 33.4 376R371C (CGC→TGC) ydcJmetalloprotein
Reads supporting (aligned to +/- strand):  ref base C (188/167);  new base T (16/5);  total (204/172)
Fisher's exact test for biased strand distribution p-value = 4.32e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 394 total aligned reads displayed.

TGGGTGGCGCTCCGGCCGAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGCGACGATGCGCAGGCCGAATAC  >  NC_002947/6011104‑6011402
                                                                                                                                                     |                                                                                                                                                      
tGGGTGGCGCTCCGGCCGAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCAc                                                                                                                                                        <  2:890629/150‑1 (MQ=255)
cgggTGGCGCTCCGGCCGAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCAc                                                                                                                                                        <  2:5587529/149‑1 (MQ=255)
  ggTGGCGCTCCGGCCGAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCAcgc                                                                                                                                                      <  1:6262238/150‑1 (MQ=255)
  ggTGGCGCTCCGGCCGAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCAcgc                                                                                                                                                      <  2:4069703/150‑1 (MQ=255)
  ggTGGCGCTCCGGCCGAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACAGTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCAcgc                                                                                                                                                      <  2:5804292/150‑1 (MQ=255)
    tGGCGCTCCGGCCGACGCCACGGCCGAGCGTTACCGGGCGCTGCTACAGGCAAACTGTGCCGAGTGCCCGGCGGACCGGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTCCGGCGCCACCGAGAAAGGCCTGGCCGCACGCGa                                                                                                                                                    <  2:3860375/150‑1 (MQ=255)
    tGGCGCTCCGGCCGAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGa                                                                                                                                                    >  2:4143923/1‑150 (MQ=255)
     ggCGCTCCGGCCGAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGAc                                                                                                                                                   >  1:3359928/1‑150 (MQ=255)
     ggCGCTCCGGCCGAAGCCAATGCCGAGCGGTACATGGCGCTGCTACAGGCAAACTTTGCCGAGGTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGAc                                                                                                                                                   <  2:1736435/150‑1 (MQ=255)
          tCCGGCCGAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGa                                                                                                                                              >  2:629003/1‑150 (MQ=255)
           ccGGCCGCAGCCAACGCCGAGCGCTACCTGGCGCTGCCACAGGCACACCTGGCCGAGTTCCCGGAGGACCTGGCGCAACGGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGaa                                                                                                                                             <  2:4747277/150‑1 (MQ=255)
           ccGGCCGAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGTCGCGTGCGGCCAggcc                                                                                                                                             <  1:4623708/150‑3 (MQ=255)
           ccGGCCGAAGCCAATCCCGAGCGTTACACGGCGCTGCCACAGGCAAACATTGCCGAGTTCCCGGATGACCTGGCGCAAACGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGaa                                                                                                                                             <  2:2482417/150‑1 (MQ=255)
              gCCGAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTACCCGGATGACCTGGTGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGgc                                                                                                                                          >  1:1039654/1‑150 (MQ=255)
                 gAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGgccgc                                                                                                                                       >  2:2394424/1‑150 (MQ=255)
                 gAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGgccgc                                                                                                                                       <  1:831183/150‑1 (MQ=255)
                  caGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCACACTTCGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGgccgcc                                                                                                                                      <  2:6418646/149‑1 (MQ=255)
                  aaGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGgccgcc                                                                                                                                      <  2:2220130/150‑1 (MQ=255)
                   aGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGccc                                                                                                                                     >  2:3395561/1‑150 (MQ=255)
                      cAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGac                                                                                                                                  >  2:6687522/1‑150 (MQ=255)
                       aaTGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGacc                                                                                                                                 >  1:2129951/1‑150 (MQ=255)
                       aaTGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGacc                                                                                                                                 >  1:6303139/1‑150 (MQ=255)
                       aaTGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGacc                                                                                                                                 >  1:5050770/1‑150 (MQ=255)
                            cGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCt                                                                                                                            >  2:2613331/1‑150 (MQ=255)
                             gAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTg                                                                                                                           >  1:3151798/1‑150 (MQ=255)
                             gAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTg                                                                                                                           >  1:6614720/1‑150 (MQ=255)
                             gAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTg                                                                                                                           >  2:1373577/1‑150 (MQ=255)
                              aGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGc                                                                                                                          >  2:1885313/1‑150 (MQ=255)
                              aGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGc                                                                                                                          >  1:4671321/1‑150 (MQ=255)
                                cGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCaa                                                                                                                        >  1:4114648/1‑150 (MQ=255)
                                cGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCaa                                                                                                                        >  2:6194293/1‑150 (MQ=255)
                                   tACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGc                                                                                                                     <  1:1904696/150‑1 (MQ=255)
                                   tACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGc                                                                                                                     <  1:5126988/150‑1 (MQ=255)
                                   tACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGc                                                                                                                     <  1:5129056/150‑1 (MQ=255)
                                      ctGGCGCGGCTACAGGCACACTGTGCCGAGGTCCCGGATGACCTGGCGCAAAGGCGCGAACAGGGCCTCGCATATTTCCGCTACGTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTg                                                                                                                  <  2:4888293/149‑1 (MQ=255)
                                       tGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGa                                                                                                                 <  1:3941330/150‑1 (MQ=255)
                                       tGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGa                                                                                                                 <  1:520729/150‑1 (MQ=255)
                                       tGGCGCTGCTACAGGCAAACTGTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGa                                                                                                                 <  2:1990903/150‑1 (MQ=255)
                                        ggCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGAt                                                                                                                >  1:1134609/1‑150 (MQ=255)
                                           gctgctACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGa                                                                                                                 <  1:1260468/146‑1 (MQ=255)
                                                tACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCgg                                                                                                        <  1:5878623/150‑1 (MQ=255)
                                                tACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCgg                                                                                                        >  1:1998187/1‑150 (MQ=255)
                                                tACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCgg                                                                                                        >  1:2899225/1‑150 (MQ=255)
                                                tACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCgg                                                                                                        >  2:1616348/1‑150 (MQ=255)
                                                    ggCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCa                                                                                                    >  1:2253259/1‑150 (MQ=255)
                                                      cAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACg                                                                                                  >  1:6155115/1‑150 (MQ=255)
                                                         aCTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGc                                                                                               >  2:774603/1‑150 (MQ=255)
                                                           tttGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCAc                                                                                             >  2:4586298/1‑150 (MQ=255)
                                                                cGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGa                                                                                        <  1:6031187/150‑1 (MQ=255)
                                                                cGAGTTCCCGGATGACCTGGCGCAAAAGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGa                                                                                        <  2:5775852/150‑1 (MQ=255)
                                                                cGAGGTCCCGGATGACCTGGCGCAAAGGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGa                                                                                        <  2:2709811/150‑1 (MQ=255)
                                                                 gAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAg                                                                                       <  2:3977932/150‑1 (MQ=255)
                                                                 gAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAg                                                                                       <  2:1266859/150‑1 (MQ=255)
                                                                   gTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGgc                                                                                     >  1:6120524/1‑150 (MQ=255)
                                                                     tCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGgcgc                                                                                   <  1:3770692/150‑1 (MQ=255)
                                                                         ggATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGgt                                                                               >  1:3236862/1‑150 (MQ=255)
                                                                          gATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGACAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCGAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGgtg                                                                              >  2:1907768/1‑150 (MQ=255)
                                                                             gACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTAc                                                                           <  1:4252961/150‑1 (MQ=255)
                                                                               ccgggCGCACCTGCGCGAACAGGGCCTCGCATATTTCCGCGACGGCGCCACCGAGACAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACGTCGAGGCGCTGGTGTACGa                                                                         <  2:5196530/147‑1 (MQ=255)
                                                                               ccTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGa                                                                         >  2:4426556/1‑150 (MQ=255)
                                                                               ccTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGa                                                                         >  2:328107/1‑150 (MQ=255)
                                                                               ccTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGa                                                                         >  1:1553829/1‑150 (MQ=255)
                                                                                cTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAg                                                                        >  1:1301399/1‑150 (MQ=255)
                                                                                 tGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAgg                                                                       <  1:2398424/150‑1 (MQ=255)
                                                                                 tGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAgg                                                                       >  2:701737/1‑150 (MQ=255)
                                                                                      cAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTc                                                                  <  2:3568041/150‑1 (MQ=255)
                                                                                      cAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTc                                                                  <  1:774603/150‑1 (MQ=255)
                                                                                          tGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGc                                                              <  1:6447090/150‑1 (MQ=255)
                                                                                             gcgAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCgg                                                           <  1:2816047/150‑1 (MQ=255)
                                                                                             gcgAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCgg                                                           >  2:2322688/1‑150 (MQ=255)
                                                                                                aaCAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGTCCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGa                                                        <  1:3575961/150‑1 (MQ=255)
                                                                                                aaCAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGa                                                        >  1:3686129/1‑150 (MQ=255)
                                                                                                aaCAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGa                                                        <  2:530404/150‑1 (MQ=255)
                                                                                                     ggCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTgcg                                                  >  2:5396233/1‑150 (MQ=255)
                                                                                                         tCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGATGTgcgggt                                               <  1:706624/150‑3 (MQ=255)
                                                                                                         tCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGcggcgg                                              <  2:6126707/150‑1 (MQ=255)
                                                                                                         tCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGcggcgg                                              <  1:4350889/150‑1 (MQ=255)
                                                                                                          cGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCggg                                             <  1:4187918/150‑1 (MQ=255)
                                                                                                              tatTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATc                                         <  2:4208762/150‑1 (MQ=255)
                                                                                                                    cGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTtccaa                                   <  1:6194293/150‑1 (MQ=255)
                                                                                                                        aCTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAg                                                                        <  1:2678367/110‑1 (MQ=255)
                                                                                                                        aCTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTtccaatcca                               >  1:5497501/1‑150 (MQ=255)
                                                                                                                         cTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTtccaatccaa                              >  1:450970/1‑150 (MQ=255)
                                                                                                                          ttCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAAc                             >  1:1801247/1‑150 (MQ=255)
                                                                                                                             gCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTg                          <  1:6799191/150‑1 (MQ=255)
                                                                                                                                aCCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGc                       >  2:3806148/1‑150 (MQ=255)
                                                                                                                                  cGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGcga                     <  2:1039654/150‑1 (MQ=255)
                                                                                                                                   gagaAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGcgac                    <  1:4718029/150‑1 (MQ=255)
                                                                                                                                       aaGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGCGACGATg                >  1:4982844/1‑150 (MQ=255)
                                                                                                                                         ttccTGGTCGCGTGCGGCCAGGAAGGCCGCCTGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGCGACGATgcg              >  1:2587738/3‑150 (MQ=255)
                                                                                                                                         ttccTGGTCGCGTGCGGCCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGCGACGATgcg              >  2:5055155/3‑150 (MQ=255)
                                                                                                                                         ttccTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGCGACGATgcg              >  1:5652705/3‑150 (MQ=255)
                                                                                                                                          tccTGGTCGCGTGCGGCCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGCGACGATgcgc             >  2:4177339/2‑150 (MQ=255)
                                                                                                                                          gCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGCGACGATgcgc             >  2:2968460/1‑150 (MQ=255)
                                                                                                                                            cTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGTTCTTCCAATCCAACCTGGGCGACGATGCGCAg           <  2:1486100/150‑1 (MQ=255)
                                                                                                                                            cTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGCGACGATGCGCAg           >  1:138761/1‑150 (MQ=255)
                                                                                                                                              ggCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGCGACGATGCGCAGGc         <  2:1558218/150‑1 (MQ=255)
                                                                                                                                                    aCGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGCGACGATGCGCAGGCCGAATa   <  2:2292657/150‑1 (MQ=255)
                                                                                                                                                     cgcgACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGCGACGATGCGCAGGCCGAATAc  <  1:4801258/150‑1 (MQ=255)
                                                                                                                                                     cgcgACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGCGACGATGCGCAGGCCGAATAc  <  1:5096615/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TGGGTGGCGCTCCGGCCGAAGCCAATGCCGAGCGTTACATGGCGCTGCTACAGGCAAACTTTGCCGAGTTCCCGGATGACCTGGCGCAAATGCGCGAACAGGGCCTCGCATATTTCCGCTACTTCGCCACCGAGAAAGGCCTGGCCGCACGCGACCAGGAAGGCCGCCCGACCACCCTGCAAGGCTTGATCGACGCGGGCCACGTGCACTTCGAGGCGCTGGTGTACGAGGACTTCCTGCCGGTGA‑GTGCGGCGGGGATCTTCCAATCCAACCTGGGCGACGATGCGCAGGCCGAATAC  >  NC_002947/6011104‑6011402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑