BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,049,245	A→G	6.1%	S350S (AGT→AGC)	PP_5297 ←	amino acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,049,245	0	A	G	6.1%	958.1 / 31.4	308	S350S (AGT→AGC)	PP_5297	amino acid transporter
Reads supporting (aligned to +/- strand): ref base A (127/161); new base G (15/4); total (143/165)
Fisher's exact test for biased strand distribution p-value = 3.77e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01

Only 100 of 312 total aligned reads displayed.

CGGCCAGGCCGGCGCAGATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGT  >  NC_002947/6049097‑6049394

cGGCCAGGCCGGCGCAGATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTAc                                                                                                                                                      >  2:6030523/1‑150 (MQ=255)

 ggCCAGGCCGGCGCAGATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACt                                                                                                                                                     <  1:4295258/150‑1 (MQ=255)

   ccAGGCCGGCGCAGATGGTGGCATAGCGCTGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCCCGAAGGTGGACTGCAGCGACGCCGCCGTCCTgg                                                                                                                                                   >  2:2515528/1‑150 (MQ=255)

   ccAGGCCGGCGCAGATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTCCCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTgg                                                                                                                                                   >  2:5926178/1‑150 (MQ=255)

      ggCCGGCGCAGATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCTTGGCCAACAGGGTGCGCGCCGGCGCCACGTAGGTTGACTGCAGCGACGCCGCCGTACTGGCCa                                                                                                                                                <  1:5909428/150‑1 (MQ=255)

            cgcAGATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGTGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACgg                                                                                                                                          <  1:1502936/150‑1 (MQ=255)

            cgcAGATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACgg                                                                                                                                          <  1:2024623/150‑1 (MQ=255)

              cAGATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCCCGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCg                                                                                                                                        >  2:5134986/1‑150 (MQ=255)

              cAGATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCg                                                                                                                                        >  2:1669976/1‑150 (MQ=255)

                gATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGAt                                                                                                                                      <  2:5264741/150‑1 (MQ=255)

                 aTGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATg                                                                                                                                     <  1:3989993/150‑1 (MQ=255)

                       gCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTGCTGGCCAGCACGGCGATGGACCTc                                                                                                                               >  1:1839865/1‑150 (MQ=255)

                        cATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCa                                                                                                                              <  1:5618229/150‑1 (MQ=255)

                        cATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGGACCGCACCGTAATACCCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCa                                                                                                                              <  2:713371/150‑1 (MQ=255)

                         aTAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAg                                                                                                                             >  2:6166797/1‑150 (MQ=255)

                             cgcgGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATc                                                                                                                         >  2:3347496/1‑150 (MQ=255)

                              gcggcgTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCg                                                                                                                        >  2:790102/1‑150 (MQ=255)

                                ggcgTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGcc                                                                                                                      <  2:6338612/150‑1 (MQ=255)

                                 gcgTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCa                                                                                                                     <  2:5368385/150‑1 (MQ=255)

                                    tttGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCg                                                                                                                  <  2:324446/150‑1 (MQ=255)

                                    tttGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGGACAGGCCACCACCGCGATGGACCACAGGATCGCCcgcg                                                                                                                  >  1:5349800/1‑150 (MQ=255)

                                         gAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGCTGGACATCAGGATCGCCAGCGGcccc                                                                                                             >  1:6035592/1‑150 (MQ=255)

                                         gAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACAACAGGATCGCCAGCGGcccc                                                                                                             >  1:2579775/1‑150 (MQ=255)

                                             gcgGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGa                                                                                                         <  2:1290860/150‑1 (MQ=255)

                                               gggCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGAcc                                                                                                       >  2:2152337/1‑150 (MQ=255)

                                                  cAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCCTGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCggg                                                                                                    <  2:4378749/150‑1 (MQ=255)

                                                   aGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGggc                                                                                                   >  2:3546539/1‑150 (MQ=255)

                                                       acTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGggccggc                                                                                               >  1:1667245/1‑150 (MQ=255)

                                                       acTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTAATGGCCACCAAGGCGATGGAAATCAGGATACCCAGCCGCCCCAACACCCggccgcc                                                                                               >  1:5500547/1‑148 (MQ=255)

                                                        cTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGggccggcc                                                                                              <  2:3603973/150‑1 (MQ=255)

                                                         tGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCCGCCCGGCGATGGACATCAGGATCGCCAGCGGCCCCCAGACCGggccggccc                                                                                             >  1:6483541/1‑149 (MQ=255)

                                                          ggCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAg                                                                                            <  1:5659516/150‑1 (MQ=255)

                                                          ggCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCAAGACCGGGCCGGCCAg                                                                                            >  1:3217289/1‑150 (MQ=255)

                                                            cGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGt                                                                                          <  1:1938015/150‑1 (MQ=255)

                                                              aaCTTCTGCGGTACCGCACCGTAATAACCCCTGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTgg                                                                                        <  2:6523479/150‑1 (MQ=255)

                                                              aaCTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTgg                                                                                        <  1:6590591/150‑1 (MQ=255)

                                                              aaCTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTgg                                                                                        <  1:4406522/150‑1 (MQ=255)

                                                                 ttCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCCGGTGGGCa                                                                                     >  2:3851810/1‑150 (MQ=255)

                                                                    tGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAg                                                                                  <  2:463594/150‑1 (MQ=255)

                                                                    tGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAg                                                                                  >  1:1394309/1‑150 (MQ=255)

                                                                    tGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACAACAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCACGTGGGCAAAg                                                                                  >  1:2673526/1‑150 (MQ=255)

                                                                     gCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGa                                                                                 >  2:5654341/1‑150 (MQ=255)

                                                                      cGGTACCGCACCGTAATAACCCTTGGCCATCTGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCTGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGAc                                                                                <  1:985235/150‑1 (MQ=255)

                                                                      cGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGAc                                                                                <  1:347313/150‑1 (MQ=255)

                                                                        gTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGt                                                                              <  1:4085662/150‑1 (MQ=255)

                                                                         tACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGtt                                                                             >  2:5900869/1‑150 (MQ=255)

                                                                            cGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTtctc                                                                          <  1:6307611/150‑1 (MQ=255)

                                                                             gCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCAAGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTcc                                                                         >  1:1201654/1‑150 (MQ=255)

                                                                              cACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCt                                                                        <  1:1135169/150‑1 (MQ=255)

                                                                              cACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCt                                                                        <  2:419008/150‑1 (MQ=255)

                                                                               aCCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTg                                                                       <  2:2830730/150‑1 (MQ=255)

                                                                                 cGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGa                                                                     <  1:1437685/150‑1 (MQ=255)

                                                                                   taataaCCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATg                                                                   >  2:4922010/1‑150 (MQ=255)

                                                                                   taataaCCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATg                                                                   >  2:3379929/1‑150 (MQ=255)

                                                                                      taaCCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATgcgc                                                                >  2:2747211/1‑150 (MQ=255)

                                                                                         cccATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCggg                                                             <  1:3925517/150‑1 (MQ=255)

                                                                                          ccATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGgt                                                            >  1:1531558/1‑150 (MQ=255)

                                                                                          ccATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGgt                                                            >  1:421334/1‑150 (MQ=255)

                                                                                          ccATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACAGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGgt                                                            >  1:6114087/1‑150 (MQ=255)

                                                                                            aTGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGgttg                                                          >  1:707403/1‑150 (MQ=255)

                                                                                              ggCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGTCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGgttgtt                                                        >  2:5733796/1‑150 (MQ=255)

                                                                                              ggCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGgttgtt                                                        <  1:2203735/150‑1 (MQ=255)

                                                                                               gCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTc                                                       <  1:126782/150‑1 (MQ=255)

                                                                                               gCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTc                                                       >  2:1745989/1‑150 (MQ=255)

                                                                                                ccAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCa                                                      >  2:5553123/1‑150 (MQ=255)

                                                                                                  aaCAGGGTGCGCGCCGGCGCCACGACGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAgg                                                    <  1:88821/150‑1 (MQ=255)

                                                                                                  aaCAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCTGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAgg                                                    >  2:753276/1‑150 (MQ=255)

                                                                                                  aaCAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAgg                                                    >  2:4728715/1‑150 (MQ=255)

                                                                                                      gggTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCa                                                <  2:1133148/150‑1 (MQ=255)

                                                                                                       ggTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAg                                               >  1:3898704/1‑150 (MQ=255)

                                                                                                            gcgcCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCg                                          >  1:512024/1‑150 (MQ=255)

                                                                                                              gcCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGtt                                        <  2:144006/150‑1 (MQ=255)

                                                                                                                 ggCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCa                                     <  1:3133082/150‑1 (MQ=255)

                                                                                                                 ggCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCa                                     <  2:3798922/150‑1 (MQ=255)

                                                                                                                  gcgcCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCAc                                    <  1:1851566/150‑1 (MQ=255)

                                                                                                                  gcgcCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCAc                                    >  2:2342907/1‑150 (MQ=255)

                                                                                                                  gcgcCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCAc                                    <  2:1259540/150‑1 (MQ=255)

                                                                                                                       aCGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACCTCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACCTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATa                               >  1:5422160/1‑150 (MQ=255)

                                                                                                                       aCGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCCAAGACGTTCTCCTGGCTGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACAGATa                               >  1:3740985/1‑150 (MQ=255)

                                                                                                                       aCGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATa                               >  2:2421180/1‑150 (MQ=255)

                                                                                                                       aCGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATa                               >  2:3474014/1‑150 (MQ=255)

                                                                                                                           aGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACGGATACCgg                           >  1:4131323/1‑150 (MQ=255)

                                                                                                                                   tGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGc                   <  1:2816986/150‑1 (MQ=255)

                                                                                                                                    gCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCa                  <  1:3561187/150‑1 (MQ=255)

                                                                                                                                    gCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCa                  <  1:1605624/150‑1 (MQ=255)

                                                                                                                                    gCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCa                  <  2:6833844/150‑1 (MQ=255)

                                                                                                                                       gCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAggg               >  2:3934757/1‑150 (MQ=255)

                                                                                                                                          aCGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTgg            <  2:2366850/150‑1 (MQ=255)

                                                                                                                                           cgccgcCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGc           <  2:1910729/150‑1 (MQ=255)

                                                                                                                                           catcgccgTGCTGGCCAGTACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGc           >  2:101028/4‑150 (MQ=255)

                                                                                                                                            gccgccGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCa                                                >  1:5698549/1‑112 (MQ=255)

                                                                                                                                            atcgccgTGCTGGCCAGTACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCg          >  2:5107479/3‑150 (MQ=255)

                                                                                                                                             tcgccgTGCTGGCCAGTACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGa         >  1:2412785/2‑150 (MQ=255)

                                                                                                                                              cgccgTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGAt        <  1:305782/150‑1 (MQ=255)

                                                                                                                                               gccgTGCTGGCCAGTACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATg       >  1:4540822/1‑150 (MQ=255)

                                                                                                                                               gccgTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATg       <  2:4965923/150‑1 (MQ=255)

                                                                                                                                                 cgTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGc     <  2:4160166/150‑1 (MQ=255)

                                                                                                                                                   tACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCgg   <  1:6182778/150‑1 (MQ=255)

                                                                                                                                                   tACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGAGGGCgg   >  1:4970628/1‑150 (MQ=255)

                                                                                                                                                    aCTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGt  <  1:2874652/150‑1 (MQ=255)

CGGCCAGGCCGGCGCAGATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGT  >  NC_002947/6049097‑6049394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`