Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,055,771 T→A 16.6% L543Q (CTG→CAG)  spoT → bifunctional (p)ppGpp synthase/hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,055,7710TA16.6% 494.0 / 17.7 209L543Q (CTG→CAG) spoTbifunctional (p)ppGpp synthase/hydrolase
Reads supporting (aligned to +/- strand):  ref base T (12/133);  new base A (24/2);  total (74/135)
Fisher's exact test for biased strand distribution p-value = 2.98e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

GGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCTGTCGGCCGGCAAGGGCATGG  >  NC_002947/6055622‑6055920
                                                                                                                                                     |                                                                                                                                                     
   aCTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCctgct                                                                                                                                                    <  2:6871106/150‑1 (MQ=255)
   aCTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCctgct                                                                                                                                                    <  2:1299349/150‑1 (MQ=255)
       aCTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCg                                                                                                                                                >  2:1496689/1‑150 (MQ=255)
       aCTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCCCcagcaggag                                                                                                                                                >  1:6141115/1‑145 (MQ=255)
         tGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGc                                                                                                                                              <  1:1317247/150‑1 (MQ=255)
         tGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCCCcagcggggggc                                                                                                                                              >  2:1234456/1‑143 (MQ=255)
           gCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCCCCCCCGGCGGTCGGccc                                                                                                                                            >  1:5046990/1‑149 (MQ=255)
            cTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCGGGGGTGGGCCGa                                                                                                                                           >  1:1518818/1‑150 (MQ=255)
            cTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCCGCGGTCGGCCgc                                                                                                                                           >  1:501572/1‑149 (MQ=255)
            cTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGACTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCGGCGGTCGGCCGa                                                                                                                                           >  2:3355345/1‑150 (MQ=255)
             ttCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCGTAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCAGCAGGGGGCCgca                                                                                                                                          >  1:3988308/1‑148 (MQ=255)
             ttCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGGCGCCCGCGGGGGGCCgca                                                                                                                                          >  2:4929721/1‑148 (MQ=255)
             ttCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGaa                                                                                                                                          <  1:1981715/150‑1 (MQ=255)
             ttCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGaa                                                                                                                                          <  1:4760479/150‑1 (MQ=255)
             ttCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGaa                                                                                                                                          <  1:3752291/150‑1 (MQ=255)
             ttCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCCCCGGCAGGCGGcccca                                                                                                                                          >  2:4368044/1‑147 (MQ=255)
             ttCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCCCCAGCGGGGGGCCgcc                                                                                                                                          >  2:1730959/1‑148 (MQ=255)
             ttCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGAGGTCGCGCGCCGCCAGCTGTCGGCCgca                                                                                                                                          >  2:1028915/1‑148 (MQ=255)
              tCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAg                                                                                                                                         <  2:141190/150‑1 (MQ=255)
                gACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCAGCTGGCGGCCGCAGGc                                                                                                                                       >  2:4551803/1‑150 (MQ=255)
                gACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCCCCGGCGGTCGGCCGCAGGc                                                                                                                                       >  2:4670104/1‑150 (MQ=255)
                gACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCCCCCGCGGGCGGCCgccggc                                                                                                                                       >  2:5762340/1‑145 (MQ=255)
                   agcagcCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGaa                                                                                                                                    <  2:2876675/150‑1 (MQ=255)
                    gcagcCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAAc                                                                                                                                   <  1:3124604/150‑1 (MQ=255)
                     cagcCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCCCCAGCGGGGGGCCGAAGGCGAACa                                                                                                                                  >  1:4566647/1‑150 (MQ=255)
                      agcCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAg                                                                                                                                 <  1:5387720/150‑1 (MQ=255)
                       gcCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCCCCCGCGGTCGGCCGGCGGCGGACacc                                                                                                                                >  1:1072103/1‑148 (MQ=255)
                        ccTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCt                                                                                                                               <  1:4228359/150‑1 (MQ=255)
                          tGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGGGCCGCCAGCAGTCGGGCCGAGGCGcacaccagc                                                                                                                             >  1:5986584/1‑145 (MQ=255)
                          tGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCCCCCGCAGTCGGCCGAAGGCGGAAagcagc                                                                                                                             >  1:2127357/1‑150 (MQ=255)
                              gaAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGgc                                                                                                                         <  2:3218818/150‑1 (MQ=255)
                                aaaTCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGgcgc                                                                                                                       <  2:3826451/150‑1 (MQ=255)
                                aaaTCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGgcgc                                                                                                                       <  1:1714838/150‑1 (MQ=255)
                                     ccccAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCGGCTGTAGGCCGAAGGCGAACAGCAGCCGGCGCCCGaa                                                                                                                  >  2:6059869/1‑150 (MQ=255)
                                     ccccAGGAACGCATCCAGTCTATTCTCGCCGAGTACAGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGaa                                                                                                                  >  1:1105503/1‑150 (MQ=255)
                                         aGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGGGCCCCCAGCGGGGGGGCGAAGGCCAACAACAGCCGGCCCCCGAAGGcc                                                                                                              >  2:2873026/1‑150 (MQ=255)
                                         aGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGcc                                                                                                              <  1:3617256/150‑1 (MQ=255)
                                            aaCGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGTGGGccttc                                                                                                           <  1:2224005/150‑4 (MQ=255)
                                            aaCGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCAc                                                                                                           <  1:6470259/150‑1 (MQ=255)
                                             aCGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACt                                                                                                          <  1:3691284/150‑1 (MQ=255)
                                             aCGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCGGCGGTCGGCCGGAGGCGCACAGCGGGCCGCGCCCGAAGGccccca                                                                                                          >  2:2984723/1‑147 (MQ=255)
                                               gCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTgg                                                                                                        <  2:5821227/150‑1 (MQ=255)
                                               gCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCGGCGGTGGGCCGCACGCCCACAGCAGCCGGCGCCACAAGccccccacg                                                                                                        >  1:6703521/1‑145 (MQ=255)
                                                 aTCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCCCCCGCGGGCGGCCGCAGGCGGCCACCGGCCCGCGCCCGCAGGCCCCCAGGCg                                                                                                      >  2:2952215/1‑150 (MQ=255)
                                                   ccAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCgcc                                                                                                    <  2:1105503/150‑3 (MQ=255)
                                                   ccAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGAt                                                                                                    <  1:5786621/150‑1 (MQ=255)
                                                    cAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCAGCTGGCGGCCGCAGGCGGACAGCGGCCGGCGCCCCAAGGCCCCCAGGCGCTc                                                                                                   >  2:5697877/1‑150 (MQ=255)
                                                      gTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGGGCCCCCGGCGGGGGGGCGCAGGCGACCCCCGGCCCGGGCCCGCAGGCCCCCCGGCgcggcg                                                                                                 >  1:6451619/1‑145 (MQ=255)
                                                         tATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCgg                                                                                              <  2:4421214/150‑1 (MQ=255)
                                                          aTTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCCCCCGCCGTCGGCCGAAGGCGGACAGCAGGCCGCCCCCGAAGGCCCACCCGCGGTCCCCGGc                                                                                             >  2:2649282/1‑150 (MQ=255)
                                                          aTTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCCCCAGCTGTGGGCCGAAGGCGCAAACCATCCGGCGCCCCGAGGCCCACAGGCGATCCGCGGc                                                                                             >  2:2648223/1‑150 (MQ=255)
                                                            tctcGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCAc                                                                                           <  1:1901751/150‑1 (MQ=255)
                                                                     gTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCt                                                                                  <  1:340860/150‑1 (MQ=255)
                                                                      tACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTg                                                                                 <  1:1760332/150‑1 (MQ=255)
                                                                      tACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTg                                                                                 <  1:1191414/150‑1 (MQ=255)
                                                                         cGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTg                                                                              <  1:2108313/150‑1 (MQ=255)
                                                                            cTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGGGCCCCCAGCCGTCGGCCCGAGGCGGACACCGGGCCGCGCCCCAAGGCCCCCAGGCGCTACGCGGCCCCCACGGCCGGGGGcgc                                                                           >  2:2497328/1‑148 (MQ=255)
                                                                             tGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCa                                                                          <  1:2535454/150‑1 (MQ=255)
                                                                             tGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCa                                                                          <  2:240591/150‑1 (MQ=255)
                                                                              ggAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAg                                                                         <  2:6524467/150‑1 (MQ=255)
                                                                              ggAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAg                                                                         <  1:4289444/150‑1 (MQ=255)
                                                                                  cTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTAc                                                                     <  1:1482998/150‑1 (MQ=255)
                                                                                    cATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTAcac                                                                   <  1:5650552/150‑3 (MQ=255)
                                                                                       agaagaCCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCaa                                                                <  2:2179133/150‑1 (MQ=255)
                                                                                       agaagaCCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCaa                                                                <  2:3652236/150‑1 (MQ=255)
                                                                                       agaagaCCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCaa                                                                <  1:2892074/150‑1 (MQ=255)
                                                                                       agaagaCCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCaa                                                                <  1:3307413/150‑1 (MQ=255)
                                                                                       agaagaCCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCaa                                                                <  2:2166779/150‑1 (MQ=255)
                                                                                                gCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCAc                                                       <  1:4483305/150‑1 (MQ=255)
                                                                                                  tCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGc                                                     <  1:532012/150‑1 (MQ=255)
                                                                                                  tCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGc                                                     <  2:5407382/150‑1 (MQ=255)
                                                                                                         cATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATccc                                              <  2:3112453/150‑1 (MQ=255)
                                                                                                          aTCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCCAATGCGAAAAGCTGCCGGCGCCACAAGGCCCACTGGCGCTACGCGGCACCGAAGGCCAGGTGCTCAGCAAAGCCAAGTGCTGGACGCCCATcccc                                             >  2:6762344/1‑149 (MQ=255)
                                                                                                          aTCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCGGCGGTCGGCCGCAGGCGCACAGCTGCCGGCGCCCGAACGCCCCCAGGCGTTCCGCGGCACCCAAGGCCGGGTGGTCACCTAAGCCCACGGCTGCAccccccgcccc                                             >  2:1979960/1‑143 (MQ=255)
                                                                                                              gCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTg                                         <  1:2147606/150‑1 (MQ=255)
                                                                                                               ccTGGGCAACCGCATGGCCTGCGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGa                                        <  1:2798590/150‑1 (MQ=255)
                                                                                                                    gCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGa                                   <  1:4113599/150‑1 (MQ=255)
                                                                                                                      aaCCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGAtt                                 <  2:933292/150‑1 (MQ=255)
                                                                                                                      aaCCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGAtt                                 <  1:4444501/150‑1 (MQ=255)
                                                                                                                        ccGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGt                               <  1:4434221/150‑1 (MQ=255)
                                                                                                                         cGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTc                              <  1:6049503/150‑1 (MQ=255)
                                                                                                                          gCATGGCCTACGTGGTCGCGCGCCGCCGGCGGTGGGCCGAAGGCGCACAGCGGCCGGCGCCACAAGGCCCCCTGGCGATCCGCGGCCCCCAAGGCCTGGTGCTCAGCTAAGCCCCGTGCGGCACCCCGCTACCCGGTGACCCGAGTgagt                             >  2:3941329/1‑147 (MQ=255)
                                                                                                                            cgggCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGc                           <  2:5227178/148‑1 (MQ=255)
                                                                                                                            aTGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGc                           <  1:5978933/150‑1 (MQ=255)
                                                                                                                            aTGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGc                           <  2:6111675/150‑1 (MQ=255)
                                                                                                                               gCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCAc                        <  2:762214/150‑1 (MQ=255)
                                                                                                                                 cTACGTGGTCGCGGGCCCCCAGCTGTCGGCCGAACGCGAACAGCTTCCGGCGCCCGAAGGCCCACTGGCGGTCCGCGGCACCCAAGGCCTGGTGCTCAGCTACGCCAAATGGTGCACGCCCATAACGGGGGACACGATTGTAGGCCAccg                      >  2:516888/1‑149 (MQ=255)
                                                                                                                                 cTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCt                      <  2:2322505/150‑1 (MQ=255)
                                                                                                                                      tGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCTGTcgg                 <  1:1601046/150‑1 (MQ=255)
                                                                                                                                       ggTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCTGTcggc                <  2:4682080/150‑1 (MQ=255)
                                                                                                                                       ggTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCTGTcggc                <  2:413540/150‑1 (MQ=255)
                                                                                                                                        gTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCTGTcggcc               <  2:5027696/150‑1 (MQ=255)
                                                                                                                                        gTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCTGTcggcc               <  2:229032/150‑1 (MQ=255)
                                                                                                                                         tCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCCCCGAACGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCCCCTGTcggccg              >  2:2566531/1‑150 (MQ=255)
                                                                                                                                         tCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCTGTcggccg              <  1:2383558/150‑1 (MQ=255)
                                                                                                                                             gcgcCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCTGTCGGCCGGCaa          <  1:6512491/150‑1 (MQ=255)
                                                                                                                                               gccgccTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCTGTCGGCCGGCAAgg        <  2:793057/150‑1 (MQ=255)
                                                                                                                                               gccgccTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCTGTCGGCCGGCAAgg        <  2:3988308/150‑1 (MQ=255)
                                                                                                                                                  gccTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCTGTCGGCCGGCAAGGGCa     >  2:1143291/1‑150 (MQ=255)
                                                                                                                                                     tgctgTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCTGTCGGCCGGCAAGGGCATgg  >  1:1058616/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAGACATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCGCGCCGCCTGCTGTCGGCCGAAGGCGAACAGCTGCCGGCGCCAGAAGGCCCACTGGCGATCCGCGGCACCGAAGGCCTGGTGCTCAGCTACGCCAAGTGCTGCACGCCGATCCCGGGTGACCCGATTGTCGGCCACCTGTCGGCCGGCAAGGGCATGG  >  NC_002947/6055622‑6055920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑