Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 867,950 A→C 11.5% intergenic (+45/+132) PP_0750 → / ← mqo‑I hypothetical protein/malate:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947867,9500AC11.5% 613.6 / 11.4 267intergenic (+45/+132)PP_0750/mqo‑Ihypothetical protein/malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (95/130);  new base C (19/21);  total (115/152)
Fisher's exact test for biased strand distribution p-value = 6.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

GACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTG  >  NC_002947/867801‑868097
                                                                                                                                                     |                                                                                                                                                         
gacctggacGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCcaca                                                                                                                                                           <  2:2727484/150‑1 (MQ=255)
gacctggacGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCcaca                                                                                                                                                           <  2:4025998/150‑1 (MQ=255)
gacctggacGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCcaca                                                                                                                                                           <  2:6119346/150‑1 (MQ=255)
acgcggccctggacctggcagagagcagcccggcggccaagcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCGCCcaca                                                                                                                                                           <  1:800847‑M1/13‑1 (MQ=255)
 cgcggccctggacctggcagagagcagcccggcggccaagcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAg                                                                                                                                                          >  2:4284209‑M1/137‑150 (MQ=255)
 cgcggccctggacctggcagagagcagcccggcggccaagcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAg                                                                                                                                                          >  2:5589003‑M1/137‑150 (MQ=255)
 acctggacGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAg                                                                                                                                                          <  1:2928141/150‑1 (MQ=255)
 acctggacGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAg                                                                                                                                                          <  2:254322/150‑1 (MQ=255)
 acctggacGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGACCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCGGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAg                                                                                                                                                          <  2:1717917/150‑1 (MQ=255)
   ctggacGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑a                                                                                                                                                   <  2:3285426/150‑1 (MQ=255)
   ctggacGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑a                                                                                                                                                   <  2:3621637/150‑1 (MQ=255)
   cggccctggacctggcagagagcagcccggcggccaagcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttgtttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCCCaggg                                                                                                                                                        <  1:6277496‑M1/16‑2 (MQ=255)
        cGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTcgc                                                                                                                                              <  1:5067060/150‑1 (MQ=255)
          gCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCgcgc                                                                                                                                            >  2:376277/1‑150 (MQ=255)
           cAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTGGCGGGCGCGCCCCCACCCCCCGG‑‑‑‑‑Aggagcccc                                                                                                                                           >  1:6374256/1‑143 (MQ=255)
           cAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTAAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGcgcc                                                                                                                                           <  2:3069233/150‑1 (MQ=255)
                  gAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTg                                                                                                                                    >  2:2189887/1‑150 (MQ=255)
                  gAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTg                                                                                                                                    <  1:1415870/150‑1 (MQ=255)
                  gAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGGGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTg                                                                                                                                    <  1:5278378/150‑1 (MQ=255)
                    gCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCCCTCCCACCGG‑‑‑‑‑AGTCGCGCCGCACCAGC‑g                                                                                                                                 >  2:6490798/1‑150 (MQ=255)
                     cACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAAGGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑gg                                                                                                                                <  1:1136949/150‑1 (MQ=255)
                       cGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGc                                                                                                                              <  1:1226552/150‑1 (MQ=255)
                        ggCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCCCGCCCCCAGG‑‑‑‑‑AGTCGCGCCGCACCGGC‑GGGCa                                                                                                                             >  1:3347807/1‑150 (MQ=255)
                          cAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCAGC‑GGGCagt                                                                                                                           >  2:4353187/1‑148 (MQ=255)
                            cccggcggccaagcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTAc                                                                                                                         >  2:868722‑M1/110‑150 (MQ=255)
                            aGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTAc                                                                                                                         <  2:330834/150‑1 (MQ=255)
                             gACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACCGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTAcc                                                                                                                        <  1:2358927/150‑1 (MQ=255)
                                cTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCGGC‑GGGCATTACCGaa                                                                                                                     >  2:1643930/1‑150 (MQ=255)
                                 ttCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCCCAGG‑‑‑‑‑AGTCGCGCCGCACCAGC‑GGGCCATACCGCAc                                                                                                                    >  2:4674651/1‑150 (MQ=255)
                                   gccaagcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCt                                                                                                                  >  1:4484455‑M1/103‑150 (MQ=255)
                                     tcgAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCtgt                                                                                                                <  2:1882473/150‑1 (MQ=255)
                                     caagcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcgcGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCtgt                                                                                                                <  1:5485937‑M1/50‑1 (MQ=255)
                                       gAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTgg                                                                                                              <  1:6484025/150‑1 (MQ=255)
                                       gAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTAGCGGGCGCGCCCGCTCCCACCGG‑‑‑‑‑CGTCGCGCCGCACCTGC‑GGGCCATAACGAACCTgggg                                                                                                              >  1:4970937/1‑150 (MQ=255)
                                       agcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTgg                                                                                                              >  2:1660887‑M1/99‑150 (MQ=255)
                                       agcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTgg                                                                                                              >  2:1245678‑M1/99‑150 (MQ=255)
                                        aGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGGCCGCTCCCACCGG‑‑‑‑‑AGTGGCGGCGCACCAGC‑GGGGCTTTACGAAACAGTggg                                                                                                             >  2:1133843/1‑150 (MQ=255)
                                        aGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCCCTCCCCCCCG‑‑‑‑‑AGGCCCGCCGCACCAGC‑GGGCCTTAACGAACCgggggg                                                                                                             >  2:1774525/1‑150 (MQ=255)
                                         cactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGa                                                                                                            >  1:1813482‑M1/97‑150 (MQ=255)
                                          cAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAg                                                                                                           <  1:1505578/150‑1 (MQ=255)
                                           ctacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGGTTCGGAGTCGCGCCGCACCTGC‑GGGCATTACCGAACCtgtg                                                                                                               >  2:973334‑M1/95‑150 (MQ=255)
                                           aGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCCCGCCCCCCGG‑‑‑‑‑AGGCGCGCCGCCCCGGC‑GGGGCCTACCGCACCGGTGGGGGc                                                                                                          >  2:800847/1‑150 (MQ=255)
                                            gATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCCCACCGGC‑GGGCATTACCCAACCGGTGGGAGCg                                                                                                         >  2:2093895/1‑150 (MQ=255)
                                             aTTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCgg                                                                                                        <  2:4914375/150‑1 (MQ=255)
                                             aTTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCCCACCAGC‑GGGCAGTACCGAACCCGGGGGGGCgg                                                                                                        >  2:191832/1‑150 (MQ=255)
                                               gcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcgtgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGcgggt                                                                                                      <  1:554574‑M1/60‑2 (MQ=255)
                                                          gcAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGaa                                                                                           <  2:2674757/150‑1 (MQ=255)
                                                           ctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcgcGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAag                                                                                          >  2:5516948‑M1/79‑150 (MQ=255)
                                                            aaTGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAaga                                                                                         <  2:1443460/150‑1 (MQ=255)
                                                            aaTGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCCCTACCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCCATACCGCACCAGTGGGAGCGGGCCTGGCcgccgaca                                                                                         >  1:4554976/1‑145 (MQ=255)
                                                               gCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGCTCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAggg                                                                                      <  2:4245730/150‑1 (MQ=255)
                                                               gCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAggg                                                                                      <  2:5835082/150‑1 (MQ=255)
                                                                ccccTGAGTACATCGAGATCATCGATACAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGc                                                                                     <  2:2793654/150‑1 (MQ=255)
                                                                ccccTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGc                                                                                     <  1:1005942/150‑1 (MQ=255)
                                                                ccccTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCGCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCGGTGGGAGCGGGCATGCCCGCGAAGAGGGc                                                                                     >  2:2760572/1‑150 (MQ=255)
                                                                 cccTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCg                                                                                    <  1:3906374/150‑1 (MQ=255)
                                                                    aaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcgcGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGa                                                                                                            >  2:5489527‑M1/70‑123 (MQ=255)
                                                                       gTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTATTCGCGGGCGCGCCCCCCCCCCCACG‑‑‑‑‑GGTCGCGCCCCCCCCGCGGGGCATTACCCAA‑CGGTGGGGGCGGGCCAGACCCCCGAGGGGGCGGTGCAg                                                                              >  2:2280112/1‑150 (MQ=255)
                                                                           aTCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCACCCACACG‑‑‑‑‑AGTCGCGCCGCACCAGC‑CGGCCATAACGAAACGGTGGGGGCGGGGCAGCCCCCGGAGAGGGCGGGAGAGGCTg                                                                          >  1:2693954/1‑150 (MQ=255)
                                                                            tCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCCCTGCCCCAGG‑‑‑‑‑AGTCGCGCCCCACCGGC‑GGGCATTACCGAAACGGTGGGAGCGGGCCAGCCCGCGAAGAGGGCGGTAAAGGCTgt                                                                         >  1:2769810/1‑149 (MQ=255)
                                                                            tCGAGATCATCGATAAAGCCCGGGGCTGCTCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGa                                                                         <  2:4537864/150‑1 (MQ=255)
                                                                             cGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑CGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGAt                                                                        <  1:1028868/150‑1 (MQ=255)
                                                                             cGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCCCGCCCCCAGG‑‑‑‑‑AGTCGCACCCCACCAGC‑GGCCCTTACCCAACCGGGGGGAGCGGGCAAGCCCGCGAAGAGGGCGGTACAGGCTgtt                                                                        >  2:2956631/1‑148 (MQ=255)
                                                                                  tcatcGATAACGCCCGGGGCTGATCTGTAGCCAGTGCCGGCCTCGACGCGGGCGCGCCCGCTCCCACCGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAgc                                                                   <  1:1477346/150‑1 (MQ=255)
                                                                                  tcatcGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAgc                                                                   <  2:3895941/150‑1 (MQ=255)
                                                                                      cGATAAAGCCGGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCa                                                               <  2:2348366/150‑1 (MQ=255)
                                                                                       gATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCCCTCCCCCACG‑‑‑‑‑AGTCGCGCCGCAACAGC‑GGGCATTACCCAACCGGTGGGAGCCGGCCATCCCCCGCAGAGGGCGGTACAAGCGGTTCAAACGCCAg                                                              >  1:5684032/1‑150 (MQ=255)
                                                                                        aTAAAGCCCGTGGCTTATCTGTAGCCTGGGCGGGCCTCTTCGCGGGCGCCCCCGCGCCCACCGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGAATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAgg                                                             <  1:2515991/150‑1 (MQ=255)
                                                                                         tAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGc                                                            <  2:6759807/150‑1 (MQ=255)
                                                                                              ccggTGGCTGATCTGGAGCCCGGGCCGGCCTCTCCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACaaa                                                       <  1:3087299/147‑1 (MQ=39)
                                                                                               ccGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACaaaa                                                      <  2:3035270/150‑1 (MQ=255)
                                                                                                    gCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGc                                                 <  2:6296276/150‑1 (MQ=255)
                                                                                                    gCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGc                                                 <  1:4333284/150‑1 (MQ=255)
                                                                                                      tGATCTGTAGCCAGTGCCGGCCTCTTGGCGGGCGCGCCCCCCCCCACCCG‑‑‑‑‑GGTCGCGCCCCCCCAGC‑GGGGCGTACCGCACCGGTGGGGGCGGGGCTGCCCCCCCACAGGGGGGGGAAGGCGGGTCAAGCCCCCGGCCCaaaaaaaaccc                                               >  2:1693765/1‑145 (MQ=255)
                                                                                                      tGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCCATAACGAACCGGGGGGGGCGGGCCTGCCCGCGGAGAGGGCGGTGCAGGCTGATCAAGCGCCCGGCACAAAAAAAGccc                                               >  2:4741457/1‑150 (MQ=38)
                                                                                                              aGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACCGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGc                                       <  2:1565039/150‑1 (MQ=255)
                                                                                                              aGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGc                                       <  2:6258180/150‑1 (MQ=255)
                                                                                                              aGCCAGTGCCGGCCTCGTCGCGGGCGCGCCCGCTCCCCCAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGc                                       <  2:5549424/150‑1 (MQ=255)
                                                                                                                ccAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGt                                     <  2:1348992/150‑1 (MQ=255)
                                                                                                                 cAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCCCAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGtt                                    <  2:5315505/150‑1 (MQ=255)
                                                                                                                    tGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACCGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGttttt                                 <  2:6342885/150‑1 (MQ=255)
                                                                                                                    ggCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGttttt                                 <  2:3244622/149‑1 (MQ=255)
                                                                                                                    ggCCGGCCTCTGCGCGGGCGCGCCCGCTCCCACCGG‑‑‑‑‑TGGCGCGCCGCACCTGC‑GGGCATCACCGCCCCTGTGGGCGCGGGATGGCCCGCGACGTGGGCGGTACAGGCTTATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGttttt                                 <  1:3484117/149‑1 (MQ=255)
                                                                                                                       cGGCCTCTTCGCGGGCGCGCCCGCACCCACACG‑‑‑‑‑AGTCGCGCCGCACCCGC‑GGGCCTTTCCGACCCGGGTGGGGCGGGCATGCCCCCGCAGCGGGCGGTACAGGCGGATGAATCGGCAGGCACAAAAAAAGCCCCGAGGGGCGGTTTTGCg                              >  2:1438073/1‑150 (MQ=255)
                                                                                                                       cGGCCTCTTCGCGGGCGCGCCCCCTCCCACAGG‑‑‑‑‑AGTCGCGCCCCACCTGC‑GGGCCATACCGAACCGGGGGGAGCGGGCATTGCCGCCAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCg                              >  2:2003560/1‑150 (MQ=255)
                                                                                                                         gCCTCTTCGCGGGCGCGCCCCCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCAATACCGAACCGGTGGGCGCGGGCCAGCCCCCGCACAGGGCGGTACACGCTGATCCAGCGCCCGGCACAAAAAACCCCCCGAGGGGCGGTTTTGCGtt                            >  2:4848737/1‑150 (MQ=255)
                                                                                                                          ccTCTTCGCGGGCGCGCCCGCACCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGACCCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCAAAAAAAACGCCCCGAGGGGCGTTTTTGCGttt                           <  1:2093895/150‑1 (MQ=255)
                                                                                                                           ctctTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTc                          <  2:3347807/150‑1 (MQ=255)
                                                                                                                           ctctTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCGGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTc                          >  2:6614716/1‑150 (MQ=255)
                                                                                                                                 gggggCGCGCCCCCTCCCCCCGG‑‑‑‑‑AGGCGCGCCGCACCGGC‑GGGCTTTACCTAACCGGTGGGAGCGGGCAGGCCCGCGACGAGGGCGTAACAGGCTGATCAAGCGCCAGGCAAAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCg                    <  1:6490823/148‑1 (MQ=255)
                                                                                                                                       gcgcCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACAGGCTTaa              >  1:3740412/1‑150 (MQ=255)
                                                                                                                                           CCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGt          <  1:2345247‑M1/150‑1 (MQ=255)
                                                                                                                                             GCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAg        <  2:5244347‑M1/150‑1 (MQ=255)
                                                                                                                                               TCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTg      <  2:2725352‑M1/150‑1 (MQ=255)
                                                                                                                                                CCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCGAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGc     >  2:1022668‑M1/1‑150 (MQ=255)
                                                                                                                                                 CCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCt    <  1:4648392‑M1/150‑1 (MQ=255)
                                                                                                                                                  CACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCtt   <  1:5879938‑M1/150‑1 (MQ=255)
                                                                                                                                                  CACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCtt   <  2:3544358‑M1/150‑1 (MQ=255)
                                                                                                                                                  CACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGGCGGGACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCtt   <  1:6670672‑M1/150‑1 (MQ=255)
                                                                                                                                                   ACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTg  <  2:2981303‑M1/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                         
GACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTGC‑GGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTG  >  NC_002947/867801‑868097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑