Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 867,985 T→G 9.4% intergenic (+80/+97) PP_0750 → / ← mqo‑I hypothetical protein/malate:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947867,9850TG 9.4% 612.3 / 3.6 254intergenic (+80/+97)PP_0750/mqo‑Ihypothetical protein/malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (79/137);  new base G (27/4);  total (111/143)
Fisher's exact test for biased strand distribution p-value = 8.51e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

CGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCG  >  NC_002947/867836‑868131
                                                                                                                                                           |                                                                                                                                                    
gccaagcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCt                                                                                                                                                      >  1:2343211‑M1/103‑150 (MQ=255)
  tcgAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCtgt                                                                                                                                                    <  2:1882473/150‑1 (MQ=255)
  caagcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcgcGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCtgt                                                                                                                                                    <  1:5485937‑M1/50‑1 (MQ=255)
  caagcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCtgt                                                                                                                                                    >  1:4806320‑M1/101‑150 (MQ=255)
   aagcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCtgtg                                                                                                                                                   >  2:394022‑M1/100‑150 (MQ=255)
    gAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTgg                                                                                                                                                  <  1:4570196/150‑1 (MQ=255)
    gAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCGGTgg                                                                                                                                                  >  2:2232424/1‑150 (MQ=255)
    agcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACCGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTgg                                                                                                                                                  >  2:207928‑M1/99‑150 (MQ=255)
    agcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTgg                                                                                                                                                  >  2:6138795‑M1/99‑150 (MQ=255)
    agcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTgg                                                                                                                                                  >  2:1245678‑M1/99‑150 (MQ=255)
    agcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTgg                                                                                                                                                  >  2:1914976‑M1/99‑150 (MQ=255)
    agcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTgg                                                                                                                                                  >  2:4694472‑M1/99‑150 (MQ=255)
     gcactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTggg                                                                                                                                                 >  1:4537224‑M1/98‑150 (MQ=255)
     aGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGGCCGCTCCCACCGG‑‑‑‑‑AGTGGCGGCGCACCAG‑CGGGGCTTTACGAAACAGTggg                                                                                                                                                 >  2:1133843/1‑150 (MQ=255)
     aGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCCCTCCCCCCCG‑‑‑‑‑AGGCCCGCCGCACCAG‑CGGGCCTTAACGAACCgggggg                                                                                                                                                 >  2:1774525/1‑150 (MQ=255)
      cactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcgcGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGa                                                                                                                                                >  2:5485937‑M1/97‑150 (MQ=255)
      cactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGa                                                                                                                                                >  2:2664450‑M1/97‑150 (MQ=255)
      cactacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcacGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGa                                                                                                                                                >  1:2631946‑M1/97‑150 (MQ=255)
        ctacgcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGGTTCGGAGTCGCGCCGCACCTG‑CGGGCATTACCGAACCtgtg                                                                                                                                                   >  2:973334‑M1/95‑150 (MQ=255)
        aGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCCCGCCCCCCGG‑‑‑‑‑AGGCGCGCCGCCCCGG‑CGGGGCCTACCGCACCGGTGGGGGc                                                                                                                                              >  2:800847/1‑150 (MQ=255)
            gcttaagctggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGGGGGAGcgggt                                                                                                                                          <  2:2364922‑M1/60‑2 (MQ=255)
            gcttaagccggtctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcgtgatcagcctgtaccgccctcttcgcgggcatGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGcgggt                                                                                                                                          <  1:554574‑M1/60‑2 (MQ=255)
               cGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCaag                                                                                                                                       >  2:3514299/1‑148 (MQ=255)
               cGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGGGGGGGCGGGCATg                                                                                                                                       >  2:487315/1‑150 (MQ=255)
                       gcAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGaa                                                                                                                               <  2:2674757/150‑1 (MQ=255)
                        ctgaaacgcaaaaacgcccctcggggcgttttttgtgcctggcgcttgatcagcctgtaccgccctcttcgcgggcgcGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAag                                                                                                                              >  2:5516948‑M1/79‑150 (MQ=255)
                         aaggCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGACCCTGTGGGAGCGGGCATGCCCGCGAaga                                                                                                                             <  2:4253165/147‑1 (MQ=255)
                         aaTGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAaga                                                                                                                             <  2:1443460/150‑1 (MQ=255)
                             ccccTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCGCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCGGTGGGAGCGGGCATGCCCGCGAAGAGGGc                                                                                                                         >  2:2760572/1‑150 (MQ=255)
                             ccccTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGGGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGc                                                                                                                         <  2:2793594/150‑1 (MQ=255)
                              cccTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCg                                                                                                                        <  1:3906374/150‑1 (MQ=255)
                              cccTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCATCGCGGGCGCGCCCCCCGCCCCCCG‑‑‑‑‑GGGGGCGCCCCACCCGCCGGGCATAACCCAACCGGGGGGGGGGGGCGGGCCCCCCACGAgggg                                                                                                                         >  1:645103/1‑149 (MQ=255)
                                     tACATCGAGATCATCGATAACGCCGGTGGCTGATGTGTCGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCCCAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGACCCTGGGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAgg                                                                                                                 <  1:1433112/150‑1 (MQ=38)
                                        aTCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCACCCACACG‑‑‑‑‑AGTCGCGCCGCACCAG‑CCGGCCATAACGAAACGGTGGGGGCGGGGCAGCCCCCGGAGAGGGCGGGAGAGGCTg                                                                                                              >  1:2693954/1‑150 (MQ=255)
                                         tCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGa                                                                                                             <  2:3084624/150‑1 (MQ=255)
                                          cGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑CGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGAt                                                                                                            <  1:1028868/150‑1 (MQ=255)
                                               tcatcTATAACGCCCGGGGCTTATCTGTAGCCAGTGCCGGCCTCTTCGGGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGGCGCGCCGCACCTG‑CGGGCATTACCGACCCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAgc                                                                                                       <  1:1187524/150‑1 (MQ=39)
                                               tcatcGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAgc                                                                                                       <  2:3895848/150‑1 (MQ=255)
                                                 atcGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCGTCGCGGGCGCGCCCCCTCCCACACG‑‑‑‑‑AGTCGCGCCGCCCCAG‑CCGGCATTACCCAACCCGTGGGAGCCGGCCAGCCCGCGCAGAGGGCGGGACCGGCGGATCAAgcg‑c                                                                                                    >  2:3312073/1‑150 (MQ=255)
                                                   cGATAAAGCCGGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCa                                                                                                  <  2:2348366/150‑1 (MQ=255)
                                                    gATAAAGCCGGTGGCTTATATGTAGCCAGTGCCGGCCTGTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCCCCGG‑CGGGCATTACCGAACCTGTGGGCGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAg                                                                                                 <  1:2397111/150‑1 (MQ=38)
                                                    gATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCCCAGG‑‑‑‑‑CGTCGCGCCGCACCTG‑CGGGCATGACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAg                                                                                                 <  2:1563789/150‑1 (MQ=255)
                                                    gATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCCCTCCCCCACG‑‑‑‑‑AGTCGCGCCGCAACAG‑CGGGCATTACCCAACCGGTGGGAGCCGGCCATCCCCCGCAGAGGGCGGTACAAGCGGTTCAAACG‑CCAg                                                                                                 >  1:5684032/1‑150 (MQ=255)
                                                    gATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTAGCGGGCGCGCCCGCTCCCACACG‑‑‑‑‑AGGCGCGCCGCACCAG‑CGGGCCGTACCGCACCGGGGGGAGCGGGCATGCCCGCGACGTGGGCGGTACAGGCTGATCAAGCG‑CCAg                                                                                                 >  2:4597727/1‑150 (MQ=255)
                                                         aGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACCGG‑‑‑‑‑GGTCGCGCCGCACCTG‑CGGGCCTTACCGAACCGGTGGGCGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑caca                                                                                           >  2:1662833/1‑150 (MQ=255)
                                                           ccggTGGCTGATCTGGAGCCCGGGCCGGCCTCTCCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACaaa                                                                                         <  1:3087299/147‑1 (MQ=39)
                                                            ccGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCCCAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACaaaa                                                                                        <  2:3035184/150‑1 (MQ=255)
                                                                 gCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGc                                                                                   <  2:6296276/150‑1 (MQ=255)
                                                                        tGTCGCCTGTGCGGGCCTGTGGGGGGGGGCGCCCGCTCCCCCCGG‑‑‑‑‑AGTCGCGCCGCACGTG‑GGGGAATTACCGAACCTGTGGGAGGGGGCTTGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAgg                                                                            <  1:1483329/150‑1 (MQ=255)
                                                                         gTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACCGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAggg                                                                           <  2:562233/150‑1 (MQ=255)
                                                                         gTAGCCAGTGCCGGCCTCTTAGCGGGCGCCCCCCCGCCCCCCCG‑‑‑‑‑GGTGCCCCCCCAACAG‑CGGGCCATACCCAACCGGGGGGCGCGGGCCAGCCCCCGCAGAGGGCGGGAACCGCGGCTCAAGCGCCCCGGCCACAAAAACCCCCCCggg                                                                             >  2:5726530/1‑148 (MQ=255)
                                                                          tAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACGGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAgggg                                                                          <  2:6843012/150‑1 (MQ=255)
                                                                              cAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCCCAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGtt                                                                      <  2:5315505/150‑1 (MQ=255)
                                                                               aGTGCCGGCCTCGTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGGGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGttt                                                                     <  2:6677350/150‑1 (MQ=255)
                                                                                 ggCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGttttt                                                                   <  2:3244622/149‑1 (MQ=255)
                                                                                 ggCCGGCCTCTGCGCGGGCGCGCCCGCTCCCACCGG‑‑‑‑‑TGGCGCGCCGCACCTG‑CGGGCATCACCGCCCCTGTGGGCGCGGGATGGCCCGCGACGTGGGCGGTACAGGCTTATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGttttt                                                                   <  1:3484117/149‑1 (MQ=255)
                                                                                    cGGCCTCTTCGCGGGCGCGCCCCCTCCCACAGG‑‑‑‑‑AGTCGCGCCCCACCTG‑CGGGCCATACCGAACCGGGGGGAGCGGGCATTGCCGCCAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCg                                                                >  2:2003560/1‑150 (MQ=255)
                                                                                     ggCCTCTTCGCGGGCGCGCCCGCTCCCACCGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATGACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGt                                                               <  2:4308728/150‑1 (MQ=255)
                                                                                      gCCTCTTCGCGGGCGCGCCCGCGCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGtt                                                              <  1:3392211/150‑1 (MQ=255)
                                                                                       ccTCTTCGCGGGCGCGCCCGCACCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGACCCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CAAAAAAAACGCCCCGAGGGGCGTTTTTGCGttt                                                             <  1:2093895/150‑1 (MQ=255)
                                                                                              gcgGGCGCGCCCGCCCCCACAGG‑‑‑‑‑TGTCGCGCCGCACCTG‑CGGGCATTACCGACCCTGTGGGAGCGGGCATGCCCGCGATGAGGGCGGTACAGGCTGATCATGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCg                                                      <  1:4349074/150‑1 (MQ=255)
                                                                                              gcgGGCGCGCCCGCACCCCCCGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCCAACGTGTGGGCGGGGGATTGCCCGCGATGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCg                                                      <  1:6490798/150‑1 (MQ=255)
                                                                                                   cgcgcCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTa                                                 >  2:4838112/1‑150 (MQ=255)
                                                                                                      GCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGc                                              >  1:6667279‑M1/1‑150 (MQ=255)
                                                                                                        CCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGt                                            <  2:2955308‑M1/150‑1 (MQ=255)
                                                                                                        CCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGt                                            <  2:5546461‑M1/150‑1 (MQ=255)
                                                                                                         CGCTCCCACCGG‑‑‑‑‑AGTCGCGCCGCACAGG‑CGGGCATTACCGAACCTGTGGGAGCGGGCAGGCCCGCGAAGAGGGCGGTACAGGCTGATCACGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTa                                           >  1:3768076‑M1/1‑150 (MQ=255)
                                                                                                         CGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCAGTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTa                                           >  2:1712474‑M1/1‑150 (MQ=255)
                                                                                                           cTCCCCCCGG‑‑‑‑‑AGTCGCGCCCCCCGCG‑GGGGCATGCCCTACCGCGGGTGCGCGGGCATGCCCGCGAAGAGGGCGGTACTGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGt                                         <  1:2358202/150‑1 (MQ=255)
                                                                                                            TCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTg                                        <  2:2725352‑M1/150‑1 (MQ=255)
                                                                                                             CCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGCACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCGAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGc                                       >  2:6374256‑M1/1‑150 (MQ=255)
                                                                                                             CCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCGAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGc                                       >  2:1022668‑M1/1‑150 (MQ=255)
                                                                                                              CCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCt                                      <  2:1355752‑M1/150‑1 (MQ=255)
                                                                                                              CCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCt                                      <  1:4648392‑M1/150‑1 (MQ=255)
                                                                                                               CACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCtt                                     <  1:5879938‑M1/150‑1 (MQ=255)
                                                                                                                cccGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTCCCGACCCTGGGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTg                                    >  1:2034393‑M1/4‑150 (MQ=255)
                                                                                                                accgg‑‑‑‑‑AGTCGCGCCGCACGTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTg                                    <  1:949161/147‑1 (MQ=255)
                                                                                                                ACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTg                                    <  2:2981303‑M1/150‑1 (MQ=255)
                                                                                                                 ccGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGAATTACCGACCCTGTGGGTGCGGGCTTGCCCGCGATGAGGGCGGTACAGGCTTATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTgg                                   >  2:4970937‑M1/3‑150 (MQ=255)
                                                                                                                    G‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTgg                                   >  1:1920768‑M1/1‑147 (MQ=255)
                                                                                                                    G‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCg                                <  1:691727‑M1/150‑1 (MQ=255)
                                                                                                                           gTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGgccgcc                              >  2:5896101/1‑150 (MQ=255)
                                                                                                                            tCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGccgccg                             >  1:3017720/1‑150 (MQ=255)
                                                                                                                              gcgcCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCggg                           >  2:524231/1‑150 (MQ=255)
                                                                                                                              gcgcCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCggg                           >  1:3977378/1‑150 (MQ=255)
                                                                                                                               cgccgcACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCCGACCGGCTTAAGCGGTGTGCTTGGCCGCCGGgc                          >  1:16549/1‑150 (MQ=255)
                                                                                                                                 ccgcACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGgctg                        >  1:2949177/1‑150 (MQ=255)
                                                                                                                                   gcACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTGTTTGCGTTTGAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGgctgct                      >  2:6580406/1‑150 (MQ=255)
                                                                                                                                        tG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTg                 <  2:3580338/150‑1 (MQ=255)
                                                                                                                                            gggCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCa              <  1:497294/150‑1 (MQ=255)
                                                                                                                                             ggCATTACCGAACCTGTGGGAGCGGGCTTGCCCGCGACGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAg             <  1:1003598/150‑1 (MQ=255)
                                                                                                                                                atgaCCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTc          <  2:4211052/147‑1 (MQ=255)
                                                                                                                                                aTTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTc          >  2:5499478/1‑150 (MQ=255)
                                                                                                                                                aTTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTc          >  1:6219850/1‑150 (MQ=255)
                                                                                                                                                aTTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTc          >  1:5619189/1‑150 (MQ=255)
                                                                                                                                                aTTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTc          >  2:2110073/1‑150 (MQ=255)
                                                                                                                                                  tcccGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCa        <  1:5284488/148‑1 (MQ=255)
                                                                                                                                                   aCCGAACCTGTGGGAGCGGGCATGCCCTCTAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAg       <  2:756254/150‑1 (MQ=255)
                                                                                                                                                    ccGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCACGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAgg      <  2:161736/150‑1 (MQ=255)
                                                                                                                                                       aaCCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGcc   >  2:6235086/1‑150 (MQ=255)
                                                                                                                                                           |                                                                                                                                                    
CGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGG‑‑‑‑‑AGTCGCGCCGCACCTG‑CGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCG‑CCAGG‑CACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCG  >  NC_002947/867836‑868131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑