Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 955,152 A→G 26.0% intergenic (+103/‑210) cyoE → / → alaC protoheme IX farnesyltransferase/aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947955,1520AG26.0% 208.0 / 92.6 146intergenic (+103/‑210)cyoE/alaCprotoheme IX farnesyltransferase/aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (56/41);  new base G (19/18);  total (75/71)
Fisher's exact test for biased strand distribution p-value = 5.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.68e-03

Only 100 of 159 total aligned reads displayed.

TGGACAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAG  >  NC_002947/955003‑955300
                                                                                                                                                      |                                                                                                                                                      
tgGACAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACaa                                                                                                                                                        <  1:1168417/150‑1 (MQ=255)
tgGACAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACaa                                                                                                                                                        <  2:5487083/150‑1 (MQ=255)
tgGACAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACaa                                                                                                                                                        <  2:6580208/150‑1 (MQ=255)
     aGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTc                                                                                                                                                   <  2:4886342/150‑1 (MQ=255)
     aGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTc                                                                                                                                                   <  1:5573061/150‑1 (MQ=255)
        cAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑g                                                                                                                                               <  2:582778/150‑1 (MQ=255)
        cAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑g                                                                                                                                               <  1:3915083/150‑1 (MQ=255)
        cAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCGACAAGGTTCCT‑g                                                                                                                                               >  2:3303943/1‑150 (MQ=255)
          gACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTa                                                                                                                                             <  1:4880572/150‑1 (MQ=255)
             cGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGa                                                                                                                                          <  2:3504839/150‑1 (MQ=255)
             cGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGa                                                                                                                                          <  2:6525733/150‑1 (MQ=255)
              gCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAAACCCCTCCAACAAGGTTCCT‑GTAGGAG‑c                                                                                                                                       >  2:5947353/1‑150 (MQ=255)
               cTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTT‑GTAGGAG‑c                                                                                                                                       <  2:2973896/150‑1 (MQ=38)
               cTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑c                                                                                                                                       <  2:3192945/150‑1 (MQ=255)
                 gCGTATGTGCTTATGACCTACGCACGATGAAACTGCTGCCTGTTTCAAGAAAACCCCGGCCACTGTGCCTGGTTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑Cgt                                                                                                                                     <  1:6536110/150‑2 (MQ=38)
                 gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTTGCGGGTAAACCCGCTCCTACAGGAACCTT‑GTAGGAG‑Cgg                                                                                                                                     <  1:6862618/150‑1 (MQ=38)
                 gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGCACCTT‑GTAGGAG‑Cgg                                                                                                                                     <  1:5598469/150‑1 (MQ=38)
                 gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTT‑GTAGGAG‑Cgg                                                                                                                                     <  2:2534014/150‑1 (MQ=38)
                 gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTT‑GTAGGAG‑Cgg                                                                                                                                     <  2:5087240/150‑1 (MQ=38)
                 gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTT‑GTAGGAG‑Cgg                                                                                                                                     <  2:3191755/150‑1 (MQ=38)
                 gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑Cgg                                                                                                                                     <  2:6543070/150‑1 (MQ=255)
                 gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑Cgg                                                                                                                                     <  2:1675872/150‑1 (MQ=255)
                 gCGGAGGTGCTGATGACCTACGCACGCTGAAACTGCGGCCTGTTTCACGAAACCCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑Cgg                                                                                                                                     <  1:3662330/150‑1 (MQ=255)
                  cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑Cggg                                                                                                                                    <  1:3412775/150‑1 (MQ=255)
                  cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTT‑CTGTGCTGGCCTCTTCACGGGTAAACCCGCTCCTACAGGAACCTT‑GTAGGAG‑CGGGt                                                                                                                                   <  1:2147007/150‑1 (MQ=25)
                     aTGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGttt                                                                                                                                 <  1:2108466/150‑1 (MQ=255)
                       gtgCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTT‑GTAGGAG‑CGGGtggtc                                                                                                                               >  1:2194068/1‑146 (MQ=37)
                       gtgCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTAc                                                                                                                               <  2:755361/150‑1 (MQ=255)
                        tgCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTAcc                                                                                                                              <  2:3501061/150‑1 (MQ=255)
                          cTGATGACCTACGCACACTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCcg                                                                                                                            >  2:5530752/1‑150 (MQ=255)
                           tgatgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTCTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTTAACCCGCTCCGACAAGGTTCCG‑GTAGGGG‑CGGGGTTACCcgc                                                                                                                           >  2:6526767/1‑150 (MQ=255)
                            gatgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGGCTCTTCGCGGGTAAAACCCCACCGACAAGGTTCGG‑GGTGGGG‑CGGGTTTTGCcgcg                                                                                                                          >  1:446686/1‑150 (MQ=255)
                              tgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGTTTCCT‑GTAGGAG‑CGGGTTTACCCGCGaa                                                                                                                        <  2:679372/150‑1 (MQ=255)
                                aCCTCCGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACGGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTGCCTGTGCCGGCCTCTTCGCGGGGAACCCCGCTCCTACCAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAaga                                                                                                                      <  2:5530742/150‑1 (MQ=38)
                                   tACGCAGGCTGATCCTGCGGCCTGTTGCACGAAAACCCCGGCCAGTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCGTGTGCGGGCCTCTTCGCGGGTAAACCCGCTCCTACACGGTTCCT‑GTAGGTG‑CGGGTTTACCCGCGAAGAGGc                                                                                                                   <  2:3455140/150‑1 (MQ=25)
                                     cgccggcTGAACCTGCGGCCTGTTACACGAACCCCCCGGCCACGGTGCGGGGGTTTTTTTTG‑GGGGGTTTTTTTCGTGGGCGGGCGTCTTCGCGGGTAAACCGGCTCC‑CCCCGGACCCTGGTCGGCG‑CGGGTTTACCCGCGAAGAGGccg                                                                                                                 <  2:6403431/145‑2 (MQ=12)
                                     cccccgcTGAACCTGCGGCCTGTGTCACGAAAACCCCGGCCCCTGTGCGGGGGATTTTTTTT‑GGGGGTATTTTACGTGGGCCGGCCTCTTCGCGGGTACCCCCGCTCCGACAAGGGACCG‑GTCGGAG‑CGGGTTTACCCGCGAAGAGGccg                                                                                                                 <  2:4151939/146‑2 (MQ=21)
                                                                cGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTAGCGGGTTAAACCCCGCCGACCAGGTTGCG‑GGGGGCGCCGGGTTTA‑CCGCCAAGAGGGCCGCCCCGCAAAATAAAAATAACGGAttt                                                                                      >  2:2314391/1‑150 (MQ=255)
                                                                  aaaaCCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑Cggg                                                                                                                                    >  1:552738/1‑102 (MQ=255)
                                                                   accccccGCCCAC‑GGGCCGGGGTTTTTTTTTGGGGGGTATTTTCCCGGGGCCGCCCCCGCCGGGGGAAACCCCCCCCCAACACGGTCCCT‑GCTGGCG‑CGGGTTCACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCa                                                                                   <  1:1469556/147‑1 (MQ=255)
                                                                          ggCCACTGTGCGGGGTTTTTTTTTT‑GGGGTTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATa                                                                            <  1:6217403/150‑1 (MQ=255)
                                                                           gCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAAACCCCGCCAACAAGGTTGCG‑GGAGGAG‑CGGGGTTACCCCCGCCGAGGCCCGCACACAAAAAAAAAAAAAACAGATTTTCAAAAAATAg                                                                           >  2:2543344/1‑150 (MQ=255)
                                                                                      ggggTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAAACCCCTCCTACAAGGTTGCG‑GGAGGAG‑CGGGGTTACCCCCGCACAGGCCCGCCCAGAAAAATAAAAATAACAGATTTTCAAAAAATATAAATGAAAAgg                                                                >  2:6007945/1‑149 (MQ=255)
                                                                                         gtattttttt‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGAGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCt                                                             <  2:6778838/147‑1 (MQ=255)
                                                                                         gTTTTTTTTT‑GGGGTTATTTTTCCTGTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACACGGTTCCT‑GTAGGAG‑CGGTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCt                                                             <  2:6336258/150‑1 (MQ=255)
                                                                                           tttttttt‑GGGGTTTTTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCtaa                                                           <  1:2226506/150‑1 (MQ=255)
                                                                                                  t‑GGGGTTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAACCCCGCTCCTACAGGGTTCGT‑GTTGGAG‑GGGTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACa                                                    <  1:2600705/150‑1 (MQ=38)
                                                                                                  t‑GGGGGTATTTTACCTGTGCCGGCCTCTTCGCGGTTAAACCCGCTCCTACCAGGTTCCT‑GTTGGCG‑CGGTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACa                                                    <  1:3285569/150‑1 (MQ=38)
                                                                                                        gTATTTTTCCTGTGCCGGCCTCGTCGCGGGGAAACCCCCACCGACAAAGGTGCG‑GTGGGAG‑CCGGGTTACCCCCCAAGAGGCCCCCCCAGAAAAATAAAAAAAACAGCTTTTAAAAAAAAAGATCAGACAAGTTCAAAGAAAACAggcac                                               >  2:1860474/1‑147 (MQ=255)
                                                                                                                    tgCCGGCCTCTTCGCGGGTAAACCCGCTCATACACGGTTCCT‑GTAGGAG‑GGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACa                                   <  2:588182/150‑1 (MQ=255)
                                                                                                                    tgCCGGCATCTTGGCGGTAAACCCCGCTCCTCAATGGTTCCT‑GTTGGTG‑CGGTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACa                                   <  1:1496866/150‑1 (MQ=255)
                                                                                                                    ggCCGGCCCCTGCGCGGGTAAACCCGCACCAACCAGGTTCCT‑GTTGTAG‑CGGTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACa                                   <  1:442749/149‑1 (MQ=255)
                                                                                                                         gCCTCTTCGCGGGTAAAACCGCTCCTACAAGGTTCCG‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCAGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATACTTTACAGGCAt                              >  2:1594386/1‑150 (MQ=255)
                                                                                                                              ttCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAAAAAAACAGGAAATTATCCTTTACAGGCAGCCTGt                         >  2:5240903/1‑150 (MQ=255)
                                                                                                                                    ggTAAACCCGCTCCTACACGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGc                   <  1:3920698/150‑1 (MQ=255)
                                                                                                                                      tAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCAc                 >  2:2794039/1‑150 (MQ=255)
                                                                                                                                       aaccccGCTCCTCCACGTTTCGT‑GTAGGCG‑CGGGTTAACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACt                <  1:3549158/147‑1 (MQ=39)
                                                                                                                                       aaaCCCGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACt                >  1:4385864/1‑150 (MQ=255)
                                                                                                                                         aCCCGCTCCTACAGGAACCTT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTGCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAt              >  1:166320/1‑150 (MQ=37)
                                                                                                                                          cccGCTCCTACCAGGTTCCT‑GTAGGAG‑CGGTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc             <  2:4249873/150‑1 (MQ=255)
                                                                                                                                          cccGCTCCTACAGGAACCTT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc             >  2:3995094/1‑150 (MQ=38)
                                                                                                                                          cccGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc             >  1:6736174/1‑150 (MQ=255)
                                                                                                                                           ccGCTCCTACAGGAACCTT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct            >  2:1495212/1‑150 (MQ=38)
                                                                                                                                           ccGCTCCTACAGGAACCTT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct            >  2:2678149/1‑150 (MQ=38)
                                                                                                                                           ccGCTCCTACAGGAACCTT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct            >  1:3018178/1‑150 (MQ=38)
                                                                                                                                           ccGCTCCTACAGGAACCTT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct            >  1:1700782/1‑150 (MQ=38)
                                                                                                                                           ccGCTCCTACAGGAACCTT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct            >  2:4430716/1‑150 (MQ=38)
                                                                                                                                           ccGCTCCTACAGGAACCTT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct            >  1:4539755/1‑150 (MQ=38)
                                                                                                                                           ccGCTCCTACAGGAACCTT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct            >  1:4388752/1‑150 (MQ=38)
                                                                                                                                           ccGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct            >  2:647419/1‑150 (MQ=255)
                                                                                                                                           ccGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct            >  2:684376/1‑150 (MQ=255)
                                                                                                                                           ccGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTGTACAGGCATCCTGTTTCGGCACTAtct            >  2:3055127/1‑150 (MQ=255)
                                                                                                                                           ccGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATAATCCTTTACAGGCATCCTGTTTCGGCACTAtct            >  2:1280899/1‑150 (MQ=255)
                                                                                                                                            cGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtctt           >  2:3465196/1‑150 (MQ=255)
                                                                                                                                             gCTCCTCCTAGGTCCCT‑GTTGGAG‑GGGTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttc          <  1:1820906/150‑1 (MQ=255)
                                                                                                                                             gCTCCTACAGGAACCTT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttc          >  2:4048758/1‑150 (MQ=38)
                                                                                                                                             gCTCCTACAGGAACCTT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttc          >  1:5882362/1‑150 (MQ=38)
                                                                                                                                             gCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTc                      <  2:5584659/138‑1 (MQ=255)
                                                                                                                                               tCCTACCAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTg        >  2:2463691/1‑150 (MQ=255)
                                                                                                                                               tCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTg        >  1:3987468/1‑150 (MQ=255)
                                                                                                                                                ccTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGa       <  1:2794039/150‑1 (MQ=255)
                                                                                                                                                ccTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGa       <  1:2794018/150‑1 (MQ=255)
                                                                                                                                                 cTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttc          >  2:2161469/1‑146 (MQ=255)
                                                                                                                                                 cTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttc          >  2:5628939/1‑146 (MQ=255)
                                                                                                                                                 cTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAt      >  1:3357870/1‑150 (MQ=255)
                                                                                                                                                  tACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt     >  2:901948/1‑150 (MQ=255)
                                                                                                                                                  tACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt     >  2:785678/1‑150 (MQ=255)
                                                                                                                                                  tACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt     >  1:5126826/1‑150 (MQ=255)
                                                                                                                                                  tACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt     >  2:3075817/1‑150 (MQ=255)
                                                                                                                                                  tACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt     >  2:5320652/1‑150 (MQ=255)
                                                                                                                                                  tACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGG‑CAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTc    >  1:5674881/1‑150 (MQ=255)
                                                                                                                                                    cAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCa   >  1:104470/1‑150 (MQ=255)
                                                                                                                                                    cAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCa   >  2:47857/1‑150 (MQ=255)
                                                                                                                                                     aaGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCa   <  1:5789330/149‑1 (MQ=255)
                                                                                                                                                     aaGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCa   >  2:5789330/1‑149 (MQ=255)
                                                                                                                                                     aaGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAg  <  2:1828402/150‑1 (MQ=255)
                                                                                                                                                     aaGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAg  >  2:1783190/1‑150 (MQ=255)
                                                                                                                                                     aaGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAg  >  1:6474171/1‑150 (MQ=255)
                                                                                                                                                     aaGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAg  >  1:5739071/1‑150 (MQ=255)
                                                                                                                                                     aaGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAg  >  1:3118461/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
TGGACAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTT‑GGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCT‑GTAGGAG‑CGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAG  >  NC_002947/955003‑955300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑