Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 961,717 C→T 7.2% G234D (GGC→GAC)  selD ← selenide, water dikinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947961,7170CT 7.2% 822.6 / 34.0 270G234D (GGC→GAC) selDselenide, water dikinase
Reads supporting (aligned to +/- strand):  ref base C (118/131);  new base T (14/5);  total (134/136)
Fisher's exact test for biased strand distribution p-value = 3.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 277 total aligned reads displayed.

TCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAGTGGTG  >  NC_002947/961568‑961866
                                                                                                                                                       |                                                                                                                                                     
tCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGc                                                                                                                                                       <  2:2985139/150‑1 (MQ=255)
  ttGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCACGACCAGGTGGccc                                                                                                                                                     >  1:4582904/1‑149 (MQ=255)
   tgtgCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTGCTCGGCCACCTGGTCGAGCTGGcc                                                                                                                                                      <  2:6581292/150‑1 (MQ=255)
    gtgCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAg                                                                                                                                                   >  2:4507776/1‑150 (MQ=255)
    gtgCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAg                                                                                                                                                   >  2:4338342/1‑150 (MQ=255)
    gtgCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAg                                                                                                                                                   <  2:1335344/150‑1 (MQ=255)
         cGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGc                                                                                                                                              <  2:6549953/150‑1 (MQ=255)
             gCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGaa                                                                                                                                          <  2:2368339/150‑1 (MQ=255)
             gCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGaa                                                                                                                                          >  2:5770594/1‑150 (MQ=255)
              cTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAg                                                                                                                                         >  1:5286733/1‑150 (MQ=255)
               tGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGc                                                                                                                                        <  2:320081/150‑1 (MQ=255)
               tGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGc                                                                                                                                        >  2:3503975/1‑150 (MQ=255)
                 tCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCg                                                                                                                                      >  1:3858951/1‑150 (MQ=255)
                 tCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCg                                                                                                                                      <  2:1505227/150‑1 (MQ=255)
                 tCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCg                                                                                                                                      >  1:1681104/1‑150 (MQ=255)
                 tCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCg                                                                                                                                      >  1:2036783/1‑150 (MQ=255)
                   gTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGGCCACCTGGTCGAGctg                                                                                                                                                >  1:5832535/1‑136 (MQ=255)
                   gTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGt                                                                                                                                    <  1:6847115/150‑1 (MQ=255)
                     aGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGa                                                                                                                                  <  2:6814172/150‑1 (MQ=255)
                           gcAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCgg                                                                                                                            >  1:4374041/1‑150 (MQ=255)
                           gcAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCgg                                                                                                                            >  2:4246349/1‑150 (MQ=255)
                             aGGGTTCCACCGGGGCTGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTc                                                                                                                          <  2:5305956/150‑1 (MQ=255)
                              gggTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCa                                                                                                                         <  2:6139265/150‑1 (MQ=255)
                                ggtCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATg                                                                                                                       <  1:690637/148‑1 (MQ=255)
                                gTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATg                                                                                                                       <  1:3389835/150‑1 (MQ=255)
                                gTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATg                                                                                                                       >  1:2996948/1‑150 (MQ=255)
                                     aCCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCtt                                                                                                                  <  1:1608433/150‑1 (MQ=255)
                                     aCCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCtt                                                                                                                  <  1:3058809/150‑1 (MQ=255)
                                       cGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGa                                                                                                                <  2:619253/150‑1 (MQ=255)
                                       cGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGa                                                                                                                <  2:1765297/150‑1 (MQ=255)
                                         gggATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGAcc                                                                                                              <  2:3022675/150‑1 (MQ=255)
                                               cAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTcc                                                                                                        >  1:1563689/1‑150 (MQ=255)
                                               cAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCACGGCCTTGACCCCGTcc                                                                                                        <  2:121128/150‑1 (MQ=255)
                                                aGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCa                                                                                                       <  2:2661336/150‑1 (MQ=255)
                                                    ttCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCACGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGttt                                                                                                   >  1:4339534/1‑150 (MQ=255)
                                                         ccAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGa                                                                                              >  2:4773686/1‑150 (MQ=255)
                                                          cAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGaa                                                                                             <  2:1092633/150‑1 (MQ=255)
                                                           aGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAg                                                                                            <  1:5624153/150‑1 (MQ=255)
                                                           aGGAAATGGACGACACAAGGCAGGCGTGGCACGGCGGCGTAGTTCACGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGACGATCAGGCCGAAGCCGCTGACGAAGGTCAAGGCCTTGAACCCGTCCAGTTTGCCGAAg                                                                                            >  2:1992839/1‑150 (MQ=255)
                                                              tAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCgg                                                                                         >  1:3632889/1‑150 (MQ=255)
                                                               aaTGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGc                                                                                        >  1:4068994/1‑150 (MQ=255)
                                                                aTGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCt                                                                                       <  2:3786931/150‑1 (MQ=255)
                                                                  ggTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGc                                                                                     >  1:794725/1‑150 (MQ=255)
                                                                    tCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCg                                                                                   >  1:1121467/1‑150 (MQ=255)
                                                                      gACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCggg                                                                                 >  1:116487/1‑150 (MQ=255)
                                                                           tAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCgtgt                                                                            >  2:2387419/1‑150 (MQ=255)
                                                                           tAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCgtgg                                                                            >  2:5733969/1‑149 (MQ=255)
                                                                              gcaggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGa                                                                         >  2:1692431/1‑150 (MQ=255)
                                                                               caggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAg                                                                        <  2:4562511/150‑1 (MQ=255)
                                                                               caggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAg                                                                        <  2:6015770/150‑1 (MQ=255)
                                                                               caggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAg                                                                        <  2:3632889/150‑1 (MQ=255)
                                                                               caggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAg                                                                        <  2:6336985/150‑1 (MQ=255)
                                                                                aggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAgg                                                                       >  2:1054498/1‑150 (MQ=255)
                                                                                aggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAgg                                                                       >  1:1243826/1‑150 (MQ=255)
                                                                                      ggCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGcac                                                                 <  1:2108131/150‑1 (MQ=255)
                                                                                       gCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGCCCACCTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGcaca                                                                >  2:4813935/1‑150 (MQ=255)
                                                                                       gCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGcaca                                                                >  1:6782890/1‑150 (MQ=255)
                                                                                            gcggcgTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTTGGCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTTACCCCGTCCAGTTTTCCGAAGCGGCTGCCGGGCGTGTTGTGGGTGGACATACAg                                                           >  1:6493423/1‑150 (MQ=255)
                                                                                              ggcgTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTc                                                         <  2:5367808/150‑1 (MQ=255)
                                                                                                 gTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTcgcg                                                      >  2:4871554/1‑150 (MQ=255)
                                                                                                 gTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTcgcg                                                      >  2:4602918/1‑150 (MQ=255)
                                                                                                 gTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTcgcg                                                      >  2:4119083/1‑150 (MQ=255)
                                                                                                  tAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCTAGCAGGCCGAAGCCGGTGACGTCGGTCAGGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGa                                                     <  2:1848594/150‑1 (MQ=255)
                                                                                                  tAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGa                                                     <  2:5168669/150‑1 (MQ=255)
                                                                                                     ttCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGcc                                                  <  2:5521630/150‑1 (MQ=255)
                                                                                                         ggTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCAAGGCCTTTACCCCGTCCAGTTTGCCGAATCGGCTGCCGGGCGTGTTGGGGGTGCACCTCCCGTCGCGAGCCAGGc                                              >  1:2289601/1‑150 (MQ=255)
                                                                                                             cgcgGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTg                                          <  1:2417696/150‑1 (MQ=255)
                                                                                                              gcgGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTgg                                         >  1:4447180/1‑150 (MQ=255)
                                                                                                                  tGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCt                                     <  1:3574656/150‑1 (MQ=255)
                                                                                                                  tGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCt                                     <  1:5500088/150‑1 (MQ=255)
                                                                                                                  tGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCt                                     <  1:161131/150‑1 (MQ=255)
                                                                                                                   gAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTg                                    <  1:6625054/150‑1 (MQ=255)
                                                                                                                   gAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTg                                    <  2:1186813/150‑1 (MQ=255)
                                                                                                                     ggCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGca                                  <  1:6101869/150‑1 (MQ=255)
                                                                                                                        cGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCacac                               >  2:5899632/1‑150 (MQ=255)
                                                                                                                          cTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGc                             >  2:3460790/1‑150 (MQ=255)
                                                                                                                           tGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCa                            <  2:1650733/150‑1 (MQ=255)
                                                                                                                              cT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAg                                                <  1:5876596/127‑1 (MQ=255)
                                                                                                                               t‑‑tCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCtt                        >  1:4153276/1‑150 (MQ=255)
                                                                                                                               t‑‑tCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCtt                        >  2:1015745/1‑150 (MQ=255)
                                                                                                                                    ggCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGc                     >  2:3536639/1‑150 (MQ=255)
                                                                                                                                     gCCTGCTCGGCCACCTGGTCGAGCTGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGcc                    >  1:965896/1‑150 (MQ=255)
                                                                                                                                     gCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGcc                    <  1:495514/150‑1 (MQ=255)
                                                                                                                                      ccAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCct                   >  1:1473363/1‑150 (MQ=255)
                                                                                                                                      ccAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCct                   >  1:5057932/1‑150 (MQ=255)
                                                                                                                                        tgCTCGGCCACCTGGTCGAGCTGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCCGCTTGGCcttc                 >  2:2844014/2‑150 (MQ=255)
                                                                                                                                        tgCTCGGCCACCTGGTCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCcttc                 >  2:77948/2‑150 (MQ=255)
                                                                                                                                        tgCTCGGCCACCTGGTCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGGTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCcttc                 >  1:4818061/2‑150 (MQ=255)
                                                                                                                                          cTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCcttctt               >  1:5508336/1‑150 (MQ=255)
                                                                                                                                             gACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTc            <  2:396987/150‑1 (MQ=255)
                                                                                                                                              aCCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCg           >  2:3912438/1‑150 (MQ=255)
                                                                                                                                               ccAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCgg          >  2:5875309/1‑150 (MQ=255)
                                                                                                                                               ccAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCgg          >  2:6703140/1‑150 (MQ=255)
                                                                                                                                                cAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGc         <  2:6418688/150‑1 (MQ=255)
                                                                                                                                                 aGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCa        >  2:1382793/1‑150 (MQ=255)
                                                                                                                                                 aGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCa        <  1:2630998/150‑1 (MQ=255)
                                                                                                                                                   gTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGc                                                                                     <  2:4360693/71‑1 (MQ=255)
                                                                                                                                                      gCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAgtggt   <  2:5041403/150‑1 (MQ=255)
                                                                                                                                                      gCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAgtggt   >  1:6326599/1‑150 (MQ=255)
                                                                                                                                                       ccGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCAAATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAgtggtg  <  1:4298260/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
TCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCT‑‑TCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAGTGGTG  >  NC_002947/961568‑961866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑