BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	100,116	T→G	5.2%	N98T (AAC→ACC)	PP_0094 ←	5'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	100,116	0	T	G	5.2%	1098.7 / 32.9	339	N98T (AAC→ACC)	PP_0094	5'‑nucleotidase
Reads supporting (aligned to +/- strand): ref base T (160/160); new base G (12/7); total (172/167)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

Only 100 of 342 total aligned reads displayed.

GAATCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAGGG  >  NC_002947/99967‑100263

gAATCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGt                                                                                                                                                     >  2:474735/1‑150 (MQ=255)

   tCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCa                                                                                                                                                  >  1:3257764/1‑150 (MQ=255)

    cAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAg                                                                                                                                                 <  1:2844488/150‑1 (MQ=255)

     aGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTTCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAgg                                                                                                                                                >  2:2303423/1‑150 (MQ=255)

      gCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAggg                                                                                                                                               >  1:4950681/1‑150 (MQ=255)

        tCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGAACGGGCAGGGcc                                                                                                                                             <  2:4676438/150‑1 (MQ=255)

          gACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTc                                                                                                                                           <  1:1356168/150‑1 (MQ=255)

          gACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTc                                                                                                                                           >  1:1276791/1‑150 (MQ=255)

          gACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGAACGGGCAGGGccgc                                                                                                                                           <  1:6455449/150‑3 (MQ=255)

            ccTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCaa                                                                                                                                         <  1:4804245/150‑1 (MQ=255)

              ttgttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGc                                                                                                                                       >  1:959432/1‑150 (MQ=255)

              ttgttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGc                                                                                                                                       >  2:6535846/1‑150 (MQ=255)

              ttgttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGc                                                                                                                                       >  2:2967840/1‑150 (MQ=255)

                gttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCaa                                                                                                                                     >  2:1697338/1‑150 (MQ=255)

                    gTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTcc                                                                                                                                 <  2:608272/150‑1 (MQ=255)

                     tGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTc                                                                                                                                                   <  2:3362085/131‑1 (MQ=255)

                        gcgTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCACGGCCTCAAGCAACTCCGGca                                                                                                                             <  1:6117383/150‑1 (MQ=255)

                          gTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGAGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGcaca                                                                                                                           <  2:4384143/150‑1 (MQ=255)

                            gCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACAcc                                                                                                                         >  2:1459158/1‑150 (MQ=255)

                               gTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTc                                                                                                                      <  2:5897671/150‑1 (MQ=255)

                               gTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTc                                                                                                                      <  1:2643247/150‑1 (MQ=255)

                               gTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTc                                                                                                                      >  2:1415982/1‑150 (MQ=255)

                                  cAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGaa                                                                                                                   <  2:358432/150‑1 (MQ=255)

                                  cAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGaa                                                                                                                   <  1:1032529/150‑1 (MQ=255)

                                   aGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAac                                                                                                                  >  1:940940/1‑150 (MQ=255)

                                   aGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAac                                                                                                                  >  2:3150514/1‑150 (MQ=255)

                                       cACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGa                                                                                                              <  1:6634377/150‑1 (MQ=255)

                                          tGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGt                                                                                                           >  1:2615239/1‑150 (MQ=255)

                                            ccGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGttt                                                                                                         >  1:5932651/1‑150 (MQ=255)

                                             cGTAGATCACCTTGAAGTGCTGATCGACCGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCCTGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGtttt                                                                                                        <  2:4434599/150‑1 (MQ=255)

                                              gTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTc                                                                                                       <  2:2615239/150‑1 (MQ=255)

                                              gTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTc                                                                                                       <  2:370952/150‑1 (MQ=255)

                                                 gATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTa                                                                                                    >  2:4268139/1‑150 (MQ=255)

                                                  aTCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTACAGGGCCTCAAGCAACTCAGGCACACCTACGAACACTAGTTTTTAGTaa                                                                                                   >  1:6806813/1‑150 (MQ=255)

                                                   tCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTaaa                                                                                                  <  2:1925058/150‑1 (MQ=255)

                                                   tCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTaaa                                                                                                  <  1:2573987/150‑1 (MQ=255)

                                                     aCCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGa                                                                                                <  1:2151624/150‑1 (MQ=255)

                                                      ccTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGAc                                                                                               <  1:1814311/150‑1 (MQ=255)

                                                      ccTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCATCGAACACGAGGTTTTCGTAAAGaa                                                                                               >  1:3734174/1‑149 (MQ=255)

                                                        ttGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCg                                                                                             >  1:5443984/1‑150 (MQ=255)

                                                          gAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGt                                                                                           <  2:5765439/150‑1 (MQ=255)

                                                               gatgatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCAcgc                                                                                      <  1:2252143/150‑1 (MQ=255)

                                                                  gatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGa                                                                                   >  1:4818326/1‑150 (MQ=255)

                                                                  gatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGa                                                                                   >  1:1869113/1‑150 (MQ=255)

                                                                   atCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGaa                                                                                  <  1:697736/150‑1 (MQ=255)

                                                                    tCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAg                                                                                 >  2:3383190/1‑150 (MQ=255)

                                                                      gAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAgcg                                                                               >  1:4044040/1‑150 (MQ=255)

                                                                       aaCGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAgcgc                                                                              >  1:4465250/1‑150 (MQ=255)

                                                                       aaCGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAgcgc                                                                              >  1:1244347/1‑150 (MQ=255)

                                                                        aCGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCt                                                                             >  2:549655/1‑150 (MQ=255)

                                                                                        gagttCGCCCGCGAAATCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAc                                                             >  2:5951219/4‑150 (MQ=255)

                                                                                        gATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAACGCTCGCGGTAGAAGTTCAc                                                             >  2:5599509/1‑150 (MQ=255)

                                                                                         aTTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                            <  1:5646870/150‑1 (MQ=255)

                                                                                         aTTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                            <  1:2725829/150‑1 (MQ=255)

                                                                                          tttCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCg                                                           >  1:1152140/1‑150 (MQ=255)

                                                                                            tCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCa                                                         <  1:2515269/150‑1 (MQ=255)

                                                                                               cgGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATcc                                                      >  1:3123788/1‑150 (MQ=255)

                                                                                                 ggCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCa                                                    <  2:2745822/150‑1 (MQ=255)

                                                                                                   cGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAgg                                                  <  1:5320456/150‑1 (MQ=255)

                                                                                                       cTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCtt                                              <  1:3309071/150‑1 (MQ=255)

                                                                                                        tCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTg                                             >  1:4569905/1‑150 (MQ=255)

                                                                                                        tCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTg                                             >  2:5687170/1‑150 (MQ=255)

                                                                                                          ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGc                                           >  2:869898/1‑150 (MQ=255)

                                                                                                          ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGc                                           >  2:6661043/1‑150 (MQ=255)

                                                                                                          ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGc                                           >  1:1686600/1‑150 (MQ=255)

                                                                                                          ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGc                                           >  1:6290131/1‑150 (MQ=255)

                                                                                                          ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGc                                           <  1:5033850/150‑1 (MQ=255)

                                                                                                          ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGc                                           >  2:5346645/1‑150 (MQ=255)

                                                                                                           cATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCt                                          >  2:718918/1‑150 (MQ=255)

                                                                                                               gTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgt                                      >  1:5847107/1‑150 (MQ=255)

                                                                                                                tttGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgtc                                     >  2:5133735/1‑150 (MQ=255)

                                                                                                                   ggAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGaa                                  >  1:3664477/1‑150 (MQ=255)

                                                                                                                    gAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAg                                 >  2:516596/1‑150 (MQ=255)

                                                                                                                     aGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGc                                >  1:6552887/1‑150 (MQ=255)

                                                                                                                       gTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTaaa                                                                                                  <  1:3910667/82‑1 (MQ=255)

                                                                                                                        tGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGt                             <  2:4557930/150‑1 (MQ=255)

                                                                                                                        tGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGt                             >  1:1964648/1‑150 (MQ=255)

                                                                                                                         ggCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTa                            <  2:6296614/150‑1 (MQ=255)

                                                                                                                         ggCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTa                            <  2:2833170/150‑1 (MQ=255)

                                                                                                                          gCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAg                           >  2:6230645/1‑150 (MQ=255)

                                                                                                                            aaTGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGaa                         >  2:4132848/1‑150 (MQ=255)

                                                                                                                            aaTGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGaa                         >  2:2445243/1‑150 (MQ=255)

                                                                                                                                tACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGc                     >  2:4010823/1‑150 (MQ=255)

                                                                                                                                   aGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATg                  >  1:301220/1‑150 (MQ=255)

                                                                                                                                     cGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGaa                >  1:4327801/1‑150 (MQ=255)

                                                                                                                                      ttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGaaa               >  2:5047747/5‑150 (MQ=255)

                                                                                                                                      ttgaggCCCTGAACGGGCAGGGCCGCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGaaa               >  1:5077052/5‑150 (MQ=255)

                                                                                                                                      ttgaggCCCTGAACGGGCAGGGCCGCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGaaa               >  1:5589711/5‑150 (MQ=255)

                                                                                                                                        gCGGCCCTGCCCGTTCAGGGCCTCGAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGc             <  1:802399/150‑1 (MQ=255)

                                                                                                                                        gCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGc             <  2:5188975/150‑1 (MQ=255)

                                                                                                                                            cccTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgc         >  2:3295839/1‑150 (MQ=255)

                                                                                                                                            cccTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgc         >  2:2227294/1‑150 (MQ=255)

                                                                                                                                            cccTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgc         <  2:2703760/150‑1 (MQ=255)

                                                                                                                                             ccTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgca        >  2:5008222/1‑150 (MQ=255)

                                                                                                                                              cggCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTGGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgcag       <  2:1291436/148‑1 (MQ=255)

                                                                                                                                              cTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgcag       >  2:1775700/1‑150 (MQ=255)

                                                                                                                                                 cccGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGcagcag    >  1:6172938/1‑150 (MQ=255)

                                                                                                                                                 cccGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGcagcag    <  2:4808086/150‑1 (MQ=255)

                                                                                                                                                   cGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAggg  <  1:3078851/150‑1 (MQ=255)

                                                                                                                                                   cGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAggg  <  1:5358385/150‑1 (MQ=255)

GAATCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAGGG  >  NC_002947/99967‑100263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`