BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	991,560	T→C	7.3%	I60T (ATC→ACC)	der →	GTPase Der

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	991,560	0	T	C	7.3%	908.8 / 40.6	300	I60T (ATC→ACC)	der	GTPase Der
Reads supporting (aligned to +/- strand): ref base T (128/149); new base C (14/8); total (142/158)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-01

Only 100 of 307 total aligned reads displayed.

CCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCACCTGCGCAAGCGGA  >  NC_002947/991411‑991709

 ccGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATc                                                                                                                                                      <  1:3196987/150‑1 (MQ=255)

 ccGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATc                                                                                                                                                      <  2:170824/150‑1 (MQ=255)

 ccGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATc                                                                                                                                                      >  2:3531082/1‑150 (MQ=255)

     aCGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCg                                                                                                                                                  <  2:3077560/150‑1 (MQ=255)

       gTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGATACCACCGGt                                                                                                                                                <  2:4166533/150‑1 (MQ=255)

         cGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTTTGGTGATGCCAGCTGGCAGGGTAGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGa                                                                                                                                              <  1:4885870/150‑1 (MQ=255)

         cGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGa                                                                                                                                              <  2:1179702/150‑1 (MQ=255)

          ggCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGATACCACCGGTgtc                                                                                                                                             <  1:28956/150‑3 (MQ=255)

             aaaTCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGaa                                                                                                                                          >  2:1791246/1‑150 (MQ=255)

               aTCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGTCACCGGTGATACCACCGGTGTCGatca                                                                                                                                        <  2:4991419/150‑4 (MQ=255)

               aTCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGt                                                                                                                                        <  1:2800703/150‑1 (MQ=255)

                  caccaTGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTgg                                                                                                                                      >  2:3357670/1‑149 (MQ=255)

                  caccaTGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTgg                                                                                                                                      <  1:3357670/149‑1 (MQ=255)

                   accaTGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGAGCCGTGACCGCCTTTTTGGTGATGCCAGCTGTCTGGGTCGTTCCGTCATCCTGATCGACACGGGTGGTATCACAGGTGACGAAGTGGGc                                                                                                                                    <  2:776964/150‑1 (MQ=255)

                    ccaTGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCa                                                                                                                                   <  1:953660/150‑1 (MQ=255)

                     caTGTTCAACCGCCTGACCACGACCCGCGATGCCATCGTCGGGGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCAt                                                                                                                                  <  1:1831505/150‑1 (MQ=255)

                     caTGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGGTGGGTCGTTGCTTCATCCTGATCGACAGCGGTGGTTTCACGGGTGTCGTAGTGGGCAt                                                                                                                                  <  1:1448582/150‑1 (MQ=255)

                      aTGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATg                                                                                                                                 >  2:1951953/1‑150 (MQ=255)

                       tGTTCAACCGCCTGACCAAGACCCGCGATGCCCTCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGGGGTATCACCGGTGACGAAGTGGGCATgg                                                                                                                                <  1:3004901/150‑1 (MQ=255)

                        gTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGa                                                                                                                               <  2:2893955/150‑1 (MQ=255)

                        gTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGa                                                                                                                               <  1:6268612/150‑1 (MQ=255)

                        gTTCAACCGCCTGACCAAGACCCGCGAGGCCATCGTCGGTGCCCTGTCGGGCCTGACCCGTGACCGCCCGTAGGGTGCTGCCAGCGGGCAGGGTCGTTCCGTCATCCTGATCGACACCGGTGGGATCACCGGTGACGAAGTGGGCATGGa                                                                                                                               <  2:4080401/150‑1 (MQ=255)

                         ttCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGAc                                                                                                                              >  1:5232023/1‑150 (MQ=255)

                               cGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGaaaaa                                                                                                                        <  1:5732977/150‑1 (MQ=255)

                               cGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGGCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGaaaaa                                                                                                                        >  2:802018/1‑150 (MQ=255)

                                gCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGaaaaaa                                                                                                                       <  2:5520605/150‑1 (MQ=255)

                                gCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGaaaaaa                                                                                                                       <  1:1014075/150‑1 (MQ=255)

                                 ccTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAAt                                                                                                                      <  2:3202053/150‑1 (MQ=255)

                                 ccTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAAt                                                                                                                      <  1:6853787/150‑1 (MQ=255)

                                  cTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATg                                                                                                                     <  1:6580688/150‑1 (MQ=255)

                                   tGACCCAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATgg                                                                                                                    <  1:2398013/150‑1 (MQ=255)

                                   tGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATgg                                                                                                                    <  2:6111285/150‑1 (MQ=255)

                                    gACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGc                                                                                                                   <  1:6401330/150‑1 (MQ=255)

                                    gACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGc                                                                                                                   <  2:2209450/150‑1 (MQ=255)

                                     aCCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGcc                                                                                                                  <  2:3916740/150‑1 (MQ=255)

                                     aCCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGcc                                                                                                                  <  1:3915485/150‑1 (MQ=255)

                                       cAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCCTGGACGAAAAAATGGCCGa                                                                                                                >  2:453888/1‑150 (MQ=255)

                                       cAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGa                                                                                                                >  2:4256181/1‑150 (MQ=255)

                                       cAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGa                                                                                                                <  2:2693651/150‑1 (MQ=255)

                                       cAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGACAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGa                                                                                                                >  2:5117917/1‑150 (MQ=255)

                                            cccGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGt                                                                                                           <  1:827977/150‑1 (MQ=255)

                                             ccGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTCTCACCGGTGACGAAGTGGGCCTGGACGAAAAAATGGCCGAGCAGTc                                                                                                          >  1:260293/1‑150 (MQ=255)

                                             ccGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTc                                                                                                          <  2:1599814/150‑1 (MQ=255)

                                               gcgATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGc                                                                                                        <  2:2988335/150‑1 (MQ=255)

                                                 gATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTc                                                                                                      <  2:6441159/150‑1 (MQ=255)

                                                        tcgtcgGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCa                                                                                               <  2:4925488/150‑1 (MQ=255)

                                                              gTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTgaag                                                                                         >  1:1352904/1‑150 (MQ=255)

                                                                gACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTgaagaa                                                                                       <  1:187329/150‑1 (MQ=255)

                                                                gACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTgaagaa                                                                                       <  2:1489854/150‑1 (MQ=255)

                                                                gACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCGGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTgaagaa                                                                                       <  2:5363743/150‑1 (MQ=255)

                                                                   cTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGcc                                                                                    >  2:1310800/1‑150 (MQ=255)

                                                                    tGTCGGGCCTGACCCGTGACCGCCAGTAGGGGGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCCCCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCg                                                                                   >  1:420056/1‑150 (MQ=255)

                                                                     gTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGa                                                                                  <  1:5465260/150‑1 (MQ=255)

                                                                      tCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGAc                                                                                 >  1:2721839/1‑150 (MQ=255)

                                                                        gggCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTa                                                                               <  2:5130866/150‑1 (MQ=255)

                                                                         ggCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTAt                                                                              <  1:2306627/150‑1 (MQ=255)

                                                                          gCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTAtg                                                                             <  1:4964334/150‑1 (MQ=255)

                                                                             tGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGc                                                                          >  1:2959386/1‑150 (MQ=255)

                                                                             tGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGc                                                                          >  2:3627429/1‑150 (MQ=255)

                                                                              gACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCt                                                                         >  1:376547/1‑150 (MQ=255)

                                                                                 ccGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGtt                                                                      >  1:2574632/1‑150 (MQ=255)

                                                                                 ccGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGtt                                                                      >  1:893705/1‑150 (MQ=255)

                                                                                 ccGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGtt                                                                      >  2:4035120/1‑150 (MQ=255)

                                                                                   gTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTcc                                                                    <  1:6460905/150‑1 (MQ=255)

                                                                                     gACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTg                                                                  <  1:6123526/150‑1 (MQ=255)

                                                                                     gACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCGTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTg                                                                  <  2:6769832/150‑1 (MQ=255)

                                                                                       ccGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGt                                                                <  1:2069956/150‑1 (MQ=255)

                                                                                        cGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGtt                                                               <  1:455088/150‑1 (MQ=255)

                                                                                        cGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGtt                                                               <  1:3666547/150‑1 (MQ=255)

                                                                                         gCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTg                                                              >  2:4715105/1‑150 (MQ=255)

                                                                                          ccAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGa                                                             >  2:584223/1‑150 (MQ=255)

                                                                                           cAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGAt                                                            <  1:5355208/150‑1 (MQ=255)

                                                                                              tATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGcc                                                         >  1:67764/1‑150 (MQ=255)

                                                                                               aTGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGccc                                                        <  1:5279616/150‑1 (MQ=255)

                                                                                                tGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCg                                                       >  2:2344267/1‑150 (MQ=255)

                                                                                                   tGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGc                                                    >  2:6347673/1‑150 (MQ=255)

                                                                                                       gCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGt                                                >  2:2377250/1‑150 (MQ=255)

                                                                                                         cAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTAt                                              <  1:4726131/150‑1 (MQ=255)

                                                                                                         cAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTAt                                              <  1:4374614/150‑1 (MQ=255)

                                                                                                                cAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCg                                       <  1:1742778/150‑1 (MQ=255)

                                                                                                                 aGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCgg                                      >  1:4499273/1‑150 (MQ=255)

                                                                                                                       gTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGAcc                                >  1:5973231/1‑150 (MQ=255)

                                                                                                                        ttCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCa                               <  1:1951953/150‑1 (MQ=255)

                                                                                                                         tCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTgaagaa                                                                                       <  1:5513916/93‑1 (MQ=255)

                                                                                                                          ccTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAga                             <  2:2571828/150‑1 (MQ=255)

                                                                                                                          ccTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAga                             <  2:706767/150‑1 (MQ=255)

                                                                                                                          ccTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAga                             <  2:1002578/150‑1 (MQ=255)

                                                                                                                          ccTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAga                             <  2:6398139/150‑1 (MQ=255)

                                                                                                                            ttCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCCTGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAgatg                           >  1:2474243/1‑150 (MQ=255)

                                                                                                                                 ccacttCGTCACCGGTGATACCACCGGTGTCGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTACTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGc                      >  1:5875386/6‑150 (MQ=255)

                                                                                                                                 ccTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGc                      >  2:5464656/1‑150 (MQ=255)

                                                                                                                                    gATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGa                   <  1:5555601/150‑1 (MQ=255)

                                                                                                                                     ttCGTCACCGGTGATACCACCGGTGTCGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAg                  >  2:443780/2‑150 (MQ=255)

                                                                                                                                      tCGTCACCGGTGATACCACCGGTGTCGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGc                 >  1:5167946/1‑150 (MQ=255)

                                                                                                                                      tCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGc                 >  2:520695/1‑150 (MQ=255)

                                                                                                                                       cGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGTGGCAGACCAGATGATCGCCGAGCa                >  2:662185/1‑150 (MQ=255)

                                                                                                                                         tcacCGGTGATACCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTgaaga                                                                                        <  2:3797597/75‑1 (MQ=255)

                                                                                                                                              ggtgatACCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCACCTgcgc         >  2:5758532/1‑150 (MQ=255)

                                                                                                                                                     tCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCACCTGCGCAAGCGGa  <  2:67764/150‑1 (MQ=255)

                                                                                                                                                     tCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCACCTGCGCAAGCGGa  <  1:4256218/150‑1 (MQ=255)

CCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCACCTGCGCAAGCGGA  >  NC_002947/991411‑991709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`