Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 104,876 G→C 8.2% P345P (CCG→CCC)  PP_0099 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947104,8760GC 8.2% 1063.3 / 63.3 328P345P (CCG→CCC) PP_0099hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (147/152);  new base C (14/13);  total (161/167)
Fisher's exact test for biased strand distribution p-value = 8.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

Only 100 of 334 total aligned reads displayed.

GAGCTGTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTC  >  NC_002947/104727‑105025
                                                                                                                                                     |                                                                                                                                                     
gAGCTGTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCg                                                                                                                                                       >  2:6704797/1‑150 (MQ=255)
gAGCTGTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCg                                                                                                                                                       <  1:938460/150‑1 (MQ=255)
   cTGTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGt                                                                                                                                                    <  1:4074966/150‑1 (MQ=255)
   cTGTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGt                                                                                                                                                    <  1:289758/150‑1 (MQ=255)
   cTGTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGt                                                                                                                                                    <  1:289937/150‑1 (MQ=255)
    tGTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTg                                                                                                                                                   <  1:443432/150‑1 (MQ=255)
    tGTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTg                                                                                                                                                   >  1:2535727/1‑150 (MQ=255)
     gTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTgg                                                                                                                                                  >  2:5235125/1‑150 (MQ=255)
      tATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTggg                                                                                                                                                 >  1:4660602/1‑150 (MQ=255)
      tATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTggg                                                                                                                                                 >  2:1558406/1‑150 (MQ=255)
      tATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTggg                                                                                                                                                 >  2:3620741/1‑150 (MQ=255)
      gaTGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGAGTGCTCGGCGTTTTGCCCACCGGGTggg                                                                                                                                                 <  2:5469281/149‑1 (MQ=255)
       aTGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGc                                                                                                                                                <  1:526439/150‑1 (MQ=255)
        tGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCa                                                                                                                                               >  2:6426575/1‑150 (MQ=255)
         gACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCCGGTGGGCaa                                                                                                                                              <  2:2424350/150‑2 (MQ=255)
         gACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCCGGTGGGCaa                                                                                                                                              <  1:6277452/150‑2 (MQ=255)
          aCAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAGGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGa                                                                                                                                             >  1:5630226/1‑150 (MQ=255)
           cAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTCTGCCCACCCGGTGGGCaaaa                                                                                                                                            <  1:4970126/150‑4 (MQ=255)
            aGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATg                                                                                                                                           >  1:1690326/1‑150 (MQ=255)
             gCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATgg                                                                                                                                          >  1:3913019/1‑150 (MQ=255)
              ccGGTTCGGCGTGCGCATGCGTGGTGACGGAGTATTTGCCGCGTGGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCGGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCCGGTGGGCaaaacgc                                                                                                                                         <  2:5150455/150‑7 (MQ=255)
               cGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTCTGCCCACCCGGTGGGCaaaacgcc                                                                                                                                        <  2:1659554/150‑8 (MQ=255)
                     ggCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTc                                                                                                                                  <  1:3724580/150‑1 (MQ=255)
                      gCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCGAGGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCt                                                                                                                                 <  2:6525995/150‑1 (MQ=255)
                        gTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGa                                                                                                                               <  1:1241928/150‑1 (MQ=255)
                        gTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCCGTTCTGa                                                                                                                               <  2:1751676/150‑1 (MQ=255)
                         tGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGAc                                                                                                                              <  1:6836622/150‑1 (MQ=255)
                          gcgcATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTTCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACa                                                                                                                             >  1:5481193/1‑150 (MQ=255)
                              aTGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGaa                                                                                                                         <  2:3339139/150‑1 (MQ=255)
                                      tgAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAaccccaccc                                                                                                                 >  1:6144115/1‑150 (MQ=255)
                                       gAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAaccccacccc                                                                                                                >  2:2366813/1‑150 (MQ=255)
                                        aaGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCAccccc                                                                                                               >  1:2068304/1‑150 (MQ=255)
                                          ggAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCca                                                                                                             >  2:794914/1‑150 (MQ=255)
                                           gAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCcac                                                                                                            >  1:3776696/1‑150 (MQ=255)
                                           gAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCcac                                                                                                            >  2:6215114/1‑150 (MQ=255)
                                             gTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAg                                                                                                          <  2:3227582/150‑1 (MQ=255)
                                              tATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAgg                                                                                                         >  2:5179062/1‑150 (MQ=255)
                                              tATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAgg                                                                                                         >  2:4626294/1‑150 (MQ=255)
                                               aTTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAggg                                                                                                        <  2:4824028/150‑1 (MQ=255)
                                               aTTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAggg                                                                                                        <  1:5755906/150‑1 (MQ=255)
                                               aTTAGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGCTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAggg                                                                                                        >  2:6018497/1‑150 (MQ=255)
                                                     cGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTgc                                                                                                  <  2:5750646/150‑1 (MQ=255)
                                                      gAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAgggggtgggg                                                                                                 <  1:6657612/150‑6 (MQ=255)
                                                        gTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCa                                                                                               <  1:3341844/150‑1 (MQ=255)
                                                         ttGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCAt                                                                                              >  1:4368175/1‑150 (MQ=255)
                                                            cTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGc                                                                                           >  2:431078/1‑150 (MQ=255)
                                                             tGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACGTGAACCCCACCCCCCACAGGGGGCTGCGCATTGCt                                                                                          >  1:2817912/1‑150 (MQ=255)
                                                             tGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCt                                                                                          >  1:6412400/1‑150 (MQ=255)
                                                              ggCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGc                                                                                                                                                >  2:291108/1‑95 (MQ=255)
                                                               gcgcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTca                                                                                        <  1:5153096/150‑1 (MQ=255)
                                                                    gcgcTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACg                                                                                   >  2:1311247/1‑150 (MQ=255)
                                                                       cTTTGCCATGTCAATGAATCGGCTGGATTTCGAAGGGCGCGAGGCGCAGGTGCTGGATTGCTCGGCGTTTTGCCCACCGTGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCaa                                                                                <  2:3962764/150‑1 (MQ=255)
                                                                       cTTTGCCAAGGCAATGAAACGGCTGGCTTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTCTGCCCACCCGGTGGGCa                                                                                                                                               >  2:3554647/1‑87 (MQ=255)
                                                                           gCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaa                                                                            >  2:857654/1‑150 (MQ=255)
                                                                            ccAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaat                                                                           <  2:3389071/150‑1 (MQ=255)
                                                                            ccAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaat                                                                           <  2:4433971/150‑1 (MQ=255)
                                                                            ccAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaat                                                                           >  1:1537981/1‑150 (MQ=255)
                                                                            ccAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaat                                                                           >  1:3109360/1‑150 (MQ=255)
                                                                              aaGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaataa                                                                         <  2:5886032/150‑1 (MQ=255)
                                                                              aaGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaataa                                                                         >  1:1156689/1‑150 (MQ=255)
                                                                              aaGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAAGGGGCTGCGCATTGCTCACAACGCAACtaataa                                                                         <  2:3405638/150‑1 (MQ=255)
                                                                               aGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGTCATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATaac                                                                        >  1:4886220/1‑150 (MQ=255)
                                                                                ggCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATaaca                                                                       >  2:3254175/1‑150 (MQ=255)
                                                                                 gCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATaacaa                                                                      >  2:4992080/1‑150 (MQ=255)
                                                                                    aTGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCa                                                                   >  1:3482528/1‑150 (MQ=255)
                                                                                    aTGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCa                                                                   >  2:3814658/1‑150 (MQ=255)
                                                                                        aaCGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCaataa                                                               >  1:4477248/1‑150 (MQ=255)
                                                                                            gCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTgc                                                           <  1:2604208/150‑1 (MQ=255)
                                                                                            gCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTgc                                                           >  1:5836548/1‑150 (MQ=255)
                                                                                             cTGGATTTCGAAGGGCGCGAGGCGCAGGGGCTGGATTGATCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTgcg                                                          <  2:3908867/150‑1 (MQ=255)
                                                                                              tGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTgcgc                                                         <  1:1021944/150‑1 (MQ=255)
                                                                                                gATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTa                                                       >  1:6551020/1‑150 (MQ=255)
                                                                                                 aTTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTaa                                                      <  2:1193886/150‑1 (MQ=255)
                                                                                                    tCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATAc                                                   <  2:3500248/150‑1 (MQ=255)
                                                                                                    tCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATAc                                                   <  2:5434752/150‑1 (MQ=255)
                                                                                                      gAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACAGGGTGGGCAGATGGCGCAGTTCTGACATGAACCACACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACat                                                 >  2:5902753/1‑150 (MQ=255)
                                                                                                       aaGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACata                                                <  1:5964984/150‑1 (MQ=255)
                                                                                                       aaGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACata                                                >  1:4271125/1‑150 (MQ=255)
                                                                                                            cgcgAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGc                                           <  2:2256947/150‑1 (MQ=255)
                                                                                                                  gcgcAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGGTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGa                                     <  1:4193104/150‑1 (MQ=255)
                                                                                                                   cgcAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGAt                                    <  1:855785/150‑1 (MQ=255)
                                                                                                                    gcAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATg                                   >  2:2762267/1‑150 (MQ=255)
                                                                                                                    gcAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATg                                   >  1:6599576/1‑150 (MQ=255)
                                                                                                                             ggATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACAAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGc                          >  2:1261817/1‑150 (MQ=255)
                                                                                                                              gATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTgc                                                                                                  <  2:2046011/77‑1 (MQ=255)
                                                                                                                               aTTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCta                        <  2:3066241/150‑1 (MQ=255)
                                                                                                                                ttGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCtat                       >  1:5379838/1‑150 (MQ=255)
                                                                                                                                   cTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTg                                      <  2:2023994/132‑1 (MQ=255)
                                                                                                                                   cTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTg                                      >  1:2023994/1‑132 (MQ=255)
                                                                                                                                    tCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATc                   >  1:740610/1‑150 (MQ=255)
                                                                                                                                    tCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATc                   >  2:1392161/1‑150 (MQ=255)
                                                                                                                                    tCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATc                   >  1:2235039/1‑150 (MQ=255)
                                                                                                                                     cGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATcc                  <  2:267101/150‑1 (MQ=255)
                                                                                                                                      ggCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCa                 >  1:5455787/1‑150 (MQ=255)
                                                                                                                                        ccatctGCCCACCCGGTGGGCAAATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGt               >  1:4914239/6‑150 (MQ=255)
                                                                                                                                         gTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGtt              >  2:5793533/1‑150 (MQ=255)
                                                                                                                                         catctGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGtt              >  1:2302494/5‑150 (MQ=255)
                                                                                                                                           tctGCCCACCCGGTGGGCAAATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATAGGCAACTGATGTTTTTTTGCTATTATCCAGttac            >  1:2512619/3‑148 (MQ=255)
                                                                                                                                            ttGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTccc           <  2:4508375/150‑1 (MQ=255)
                                                                                                                                                 cACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATGGCGCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCCCCTGATGTTTTTTTGCTATTATCCAGTTCCCGCCtt      >  2:4935237/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAGCTGTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTC  >  NC_002947/104727‑105025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑