BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,250,285	G→C	7.4%	A66A (GCC→GCG)	pyrC ←	dihydroorotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,250,285	0	G	C	7.4%	983.1 / 50.8	296	A66A (GCC→GCG)	pyrC	dihydroorotase
Reads supporting (aligned to +/- strand): ref base G (138/136); new base C (12/10); total (150/146)
Fisher's exact test for biased strand distribution p-value = 8.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 318 total aligned reads displayed.

CTGGTCACGCCGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCACCATCGCGCAG  >  NC_002947/1250136‑1250434

 tGGTCACGCCGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTgccgg                                                                                                                                                      >  2:40816/1‑150 (MQ=255)

   gTCACGCCGGACTCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTgccggcc                                                                                                                                                    <  2:6196048/150‑1 (MQ=255)

     cACGCCGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGccggccgg                                                                                                                                                  <  1:6363546/150‑1 (MQ=255)

      aCGCCGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCcggg                                                                                                                                                 >  2:3214679/1‑150 (MQ=255)

        gCCGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCcgggcg                                                                                                                                               >  1:3733583/1‑150 (MQ=255)

        gCCGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCcgggcg                                                                                                                                               >  1:2571529/1‑150 (MQ=255)

         ccGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCcgggcgg                                                                                                                                              <  1:2018908/150‑1 (MQ=255)

          cGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCcgggcggg                                                                                                                                             <  2:4879501/150‑1 (MQ=255)

          cGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCcgggcggg                                                                                                                                             <  2:2803/150‑1 (MQ=255)

          cGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCCGCccggccgg                                                                                                                                             <  1:6797908/150‑1 (MQ=255)

            gAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCa                                                                                                                                           >  2:506639/1‑150 (MQ=255)

               tCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGcc                                                                                                                                        >  2:2085312/1‑150 (MQ=255)

                  gAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCTCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGa                                                                                                                                     >  2:5298039/1‑150 (MQ=255)

                   aGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGaa                                                                                                                                    <  2:6306054/150‑1 (MQ=255)

                   aGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCCGCCCGGCCGGCAGccgctt                                                                                                                                    <  1:4925064/150‑5 (MQ=255)

                   aGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCCGCCCGGCCGGCAGccgctt                                                                                                                                    <  2:2149025/150‑5 (MQ=255)

                    gTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAAt                                                                                                                                   <  1:5899848/150‑1 (MQ=255)

                      tggtggtggCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCCGCCCGGCCGGCAGccgcttcga                                                                                                                                 <  1:344211/150‑8 (MQ=255)

                        gtggtggCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATgcgc                                                                                                                               <  2:5799592/150‑1 (MQ=255)

                        gtggtggCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATgcgc                                                                                                                               <  2:517212/150‑1 (MQ=255)

                        gtggtggCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATgcgc                                                                                                                               >  1:4029850/1‑150 (MQ=255)

                         tggtggCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCt                                                                                                                              >  1:6542926/1‑150 (MQ=255)

                          ggtggCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTc                                                                                                                             >  2:2630209/1‑150 (MQ=255)

                           gtggCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCa                                                                                                                            >  2:3944156/1‑150 (MQ=255)

                            tggCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCAc                                                                                                                           >  1:6697445/1‑150 (MQ=255)

                            tggCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCAc                                                                                                                           >  1:5959974/1‑150 (MQ=255)

                             ggCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACg                                                                                                                          >  2:2872149/1‑150 (MQ=255)

                             ggCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACg                                                                                                                          <  2:4445445/150‑1 (MQ=255)

                              gcgcCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACgg                                                                                                                         >  2:3013196/1‑150 (MQ=255)

                              gcgcCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACgg                                                                                                                         <  2:5967103/150‑1 (MQ=255)

                                gccggccgGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTa                                                                                                                       <  1:968906/150‑1 (MQ=255)

                                gccggccgGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTa                                                                                                                       <  1:3213184/150‑1 (MQ=255)

                                   ggccggGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGgc                                                                                                                    <  2:6832406/150‑1 (MQ=255)

                                   ggccggGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGgc                                                                                                                    >  1:3170775/1‑150 (MQ=255)

                                             aaCTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCg                                                                                                          >  2:3693760/1‑150 (MQ=255)

                                              aCTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCgg                                                                                                         >  1:2933918/1‑150 (MQ=255)

                                              aCTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTAgg                                                                                                         <  1:5359579/150‑1 (MQ=255)

                                                   gcggcgTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGagc                                                                                                    <  2:1961464/150‑3 (MQ=255)

                                                   gcggcgTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACa                                                                                                    >  1:4305972/1‑150 (MQ=255)

                                                   gcggcgTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACa                                                                                                    <  1:5221836/150‑1 (MQ=255)

                                                     ggcgTTCACGAAACCGCTGGCGTTGGCCGGGCTGACGTCCTCGGGGGTGGTTCGGTCGGTTTGGTACATCAGCATCAGCGGCTCGAAGAGGCTGCAGTCCGGGCGGTCAGCCAGAATGAGCTCACGGTAGGCGCCGGCCTCGGTGACAtt                                                                                                  <  1:2291302/150‑1 (MQ=255)

                                                         tACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTgcgc                                                                                              >  1:4420942/1‑150 (MQ=255)

                                                           cacGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCAc                                                                                            >  2:1671420/1‑150 (MQ=255)

                                                           cacGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCAc                                                                                            >  1:5726684/1‑150 (MQ=255)

                                                            acGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACg                                                                                           >  1:1473527/1‑150 (MQ=255)

                                                            acGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACg                                                                                           >  1:3955964/1‑150 (MQ=255)

                                                               aaaCCGCTGGTCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGggc                                                                                        <  1:6059126/150‑1 (MQ=255)

                                                                  ccGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGggcggc                                                                                     <  1:1833169/150‑1 (MQ=255)

                                                                          cTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGtt                                                                             <  2:3791264/150‑1 (MQ=255)

                                                                           ttGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTg                                                                            <  1:4853911/150‑1 (MQ=255)

                                                                            tGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTgg                                                                           <  1:5648344/150‑1 (MQ=255)

                                                                            tGGCCCCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTgg                                                                           <  1:2995774/150‑1 (MQ=255)

                                                                             ggCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTggg                                                                          <  1:2158962/150‑1 (MQ=255)

                                                                              gCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTTCAGCACGATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGc                                                                         <  1:1601463/150‑1 (MQ=255)

                                                                                cgcgcgGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCat                                                                       <  1:4106365/150‑1 (MQ=255)

                                                                                cgcgcgGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCat                                                                       <  1:5067428/150‑1 (MQ=255)

                                                                                  cgcgGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCatga                                                                     <  2:2782126/150‑1 (MQ=255)

                                                                                   gcgGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCg                                                                                                                <  2:4570867/106‑1 (MQ=255)

                                                                                    cgGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCatgatg                                                                   <  2:1399989/150‑1 (MQ=255)

                                                                                      gACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCatgatgat                                                                 >  2:6024516/1‑150 (MQ=255)

                                                                                      gACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCatgatgat                                                                 <  1:1107671/150‑1 (MQ=255)

                                                                                      gACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCatgatgat                                                                 <  1:1302821/150‑1 (MQ=255)

                                                                                       aCGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCatgatgatg                                                                <  1:747527/150‑1 (MQ=255)

                                                                                         gTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGc                                                              <  2:6409443/150‑1 (MQ=255)

                                                                                            cTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACg                                                           >  1:4187904/1‑150 (MQ=255)

                                                                                            cTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACg                                                           <  1:5490898/150‑1 (MQ=255)

                                                                                            cTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACg                                                           >  2:6125488/1‑150 (MQ=255)

                                                                                             tCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACgg                                                          <  1:4557830/150‑1 (MQ=255)

                                                                                             tCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACgg                                                          >  1:1400176/1‑150 (MQ=255)

                                                                                             tCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACgg                                                          <  2:3469278/150‑1 (MQ=255)

                                                                                                  gCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGaa                                                     <  2:3410325/150‑1 (MQ=255)

                                                                                                  gCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGaa                                                     <  2:4267242/150‑1 (MQ=255)

                                                                                                  gCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGaa                                                     <  1:1806686/150‑1 (MQ=255)

                                                                                                   cTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAg                                                    <  1:1008725/150‑1 (MQ=255)

                                                                                                   cTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAg                                                    <  2:2560363/150‑1 (MQ=255)

                                                                                                      gTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTa                                                 <  1:2734831/150‑1 (MQ=255)

                                                                                                        gCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTAcg                                               >  2:1779276/1‑150 (MQ=255)

                                                                                                          ggtcggtGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTAcgcg                                             >  1:3300111/1‑150 (MQ=255)

                                                                                                              ggtGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCAc                                         >  2:899867/1‑150 (MQ=255)

                                                                                                                tGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACga                                       >  1:3503352/1‑149 (MQ=255)

                                                                                                                 gAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTc                                      <  1:5293244/150‑1 (MQ=255)

                                                                                                                 gAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTc                                      <  2:5564544/150‑1 (MQ=255)

                                                                                                                 gAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTc                                      <  1:4747322/150‑1 (MQ=255)

                                                                                                                        aGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACg                               <  1:996593/150‑1 (MQ=255)

                                                                                                                         gCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCACCgg                              >  2:845915/1‑150 (MQ=255)

                                                                                                                             cATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGtgtg                          >  1:5175573/1‑150 (MQ=255)

                                                                                                                              aTCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTgg                         >  1:4455713/1‑150 (MQ=255)

                                                                                                                              aTCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTgg                         >  2:4815212/1‑150 (MQ=255)

                                                                                                                                 aGCGGCTCGAAGCTGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCTGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGc                      <  2:6491589/150‑1 (MQ=255)

                                                                                                                                  gCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGgt                             <  1:2507755/142‑1 (MQ=255)

                                                                                                                                     gCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGa                  >  2:5469107/1‑150 (MQ=255)

                                                                                                                                       tCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACg                >  2:4949745/1‑150 (MQ=255)

                                                                                                                                          ttctggCTGCCCGCCCGGCCGGCAGCCGGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCa             >  2:6545305/5‑150 (MQ=255)

                                                                                                                                            ctggCTGCCCGCCCGGCCGGCAGCCGGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCAcc           >  1:4177650/3‑150 (MQ=255)

                                                                                                                                             tggCTGCCCGCCCGGCCGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCACCa          >  1:4771861/2‑150 (MQ=255)

                                                                                                                                             tggCTGCCCGCCCGGCCGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCACCa          >  2:3313458/2‑150 (MQ=255)

                                                                                                                                             cGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCACCa          >  1:6270133/1‑150 (MQ=255)

                                                                                                                                               gCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCACCATc        >  2:5737162/1‑150 (MQ=255)

                                                                                                                                                    cggccggGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCACCATCGCGCa   >  1:3057303/1‑150 (MQ=255)

                                                                                                                                                     ggccggGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCACCATCGCGCAg  >  1:4278575/1‑150 (MQ=255)

CTGGTCACGCCGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCACCATCGCGCAG  >  NC_002947/1250136‑1250434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`