BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,385,677	G→T	5.5%	intergenic (‑110/+25)	PP_t67 ← / ← PP_t66	tRNA‑Met/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,385,677	0	G	T	5.5%	785.3 / 23.1	248	intergenic (‑110/+25)	PP_t67/PP_t66	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base G (129/101); new base T (6/9); total (137/111)
Fisher's exact test for biased strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

TTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTA  >  NC_002947/1385529‑1385824

     aGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACa                                                                                                                                                <  2:3863243/150‑1 (MQ=255)

        ccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                             <  1:432291/150‑1 (MQ=255)

         cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTgg                                                                                                                                            <  1:2773256/150‑1 (MQ=255)

          gacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGc                                                                                                                                           <  1:3228172/150‑1 (MQ=255)

           acgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGcc                                                                                                                                          <  2:4942278/150‑1 (MQ=255)

            cgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑t                                                                                                                                        <  2:5795081/150‑1 (MQ=255)

             gaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACGCAGTGGCC‑tt                                                                                                                                       <  2:5204594/150‑1 (MQ=18)

              aGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGCC‑ttt                                                                                                                                      <  1:1958850/150‑1 (MQ=255)

                cTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGTATTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGTCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTc                                                                                                                                    <  2:1583634/150‑1 (MQ=255)

                    cAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGGGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGAGGTTTTTTCGCGAAAGCTAATCAGACGTCAAGGAAAATGGGGAGGGTGTTAAAAGTGTCC‑TATTCTTGa                                                                                                                                >  2:5871050/1‑150 (MQ=17)

                       gctgctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATc                                                                                                                             >  1:5078718/1‑150 (MQ=255)

                       gctgctCCATCCCGCGTCAGTGGGGCGGATTCTACAGGGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGAAGTTTTTTCGAGAATGCTTATCATCACGAAAATAAAAAGGACACTGTCTTAACAGTGGAA‑TTATCTTGAATc                                                                                                                             >  1:105056/1‑150 (MQ=17)

                          gctCCCTCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTAGGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTgg                                                                                                                          <  2:3612891/150‑1 (MQ=255)

                          gctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTgg                                                                                                                          <  2:4302966/150‑1 (MQ=255)

                            tCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTTTATGTCAAGAGCTAGGCTTTGATTTTTCGACGTTTTTTCGCTAATGCTTATCAGCAGGCAAAGAAATAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGtt                                                                                                                        >  1:779001/1‑150 (MQ=255)

                            tCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGtt                                                                                                                        <  1:6825658/150‑1 (MQ=255)

                            tCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGtt                                                                                                                        <  1:1832727/150‑1 (MQ=255)

                            tCCATCCCGCGTCAGTGGGGCGGATTCTACAGGGTGTGGTTTGTATGTCAAGAGCTCGGGGTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAAGCGGGtg                                                                                                                        >  2:779910/1‑149 (MQ=18)

                             ccATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TGTTCTTGAATCTGGTTg                                                                                                                       >  2:5262778/1‑150 (MQ=255)

                               aTCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCg                                                                                                                     >  1:5246974/1‑150 (MQ=255)

                                  ccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGa                                                                                                                  >  2:3092088/1‑150 (MQ=255)

                                  ccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAACAAAATGCCCACAGACATAACAGTGCCC‑AAATCTGGAATCACGTGGCGGGa                                                                                                                  >  1:2649546/1‑150 (MQ=18)

                                    gcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGc                                                                                                                >  1:6513076/1‑150 (MQ=255)

                                    gcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATAAGCAGGCAAAGAAAAAGGCCAAAGTGTTAACACAGGCC‑TTTTCTAGAATCTGGTTGAGGGAGc                                                                                                                >  1:1546116/1‑150 (MQ=18)

                                       tCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCgg                                                                                                             >  2:160319/1‑150 (MQ=255)

                                        cAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGa                                                                                                            <  2:3304736/150‑1 (MQ=255)

                                        cAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAAGGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGa                                                                                                            <  2:1857844/150‑1 (MQ=255)

                                         aGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGAt                                                                                                           >  2:6193057/1‑150 (MQ=255)

                                           tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGAttt                                                                                                         <  1:3571504/150‑1 (MQ=255)

                                             gggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGa                                                                                                       >  1:6407301/1‑150 (MQ=255)

                                             gggCGGATTCTACAGTGTGTGGTTGGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTGCGCGAATGCGTATCAGCAGGCAAAGAACAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGa                                                                                                       <  1:2438716/150‑1 (MQ=255)

                                               gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                                     <  2:2433705/150‑1 (MQ=255)

                                               gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                                     <  2:3810222/150‑1 (MQ=255)

                                                cGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAcc                                                                                                    <  2:1167420/150‑1 (MQ=255)

                                                   aTTCTACAGTTTTTGGTTTTTATTTCAACCTCTAGGCTTTGTTTTTTCGACGTTTTTTCTCGATTTATTATCAGCATGCAAAGAAAAAGGGCACTGTGTTAACTGGGTCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcgac                                                                                                 <  2:2651697/150‑1 (MQ=18)

                                                       tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCAGGATTTGAACCGACGAcc                                                                                             >  2:5974927/1‑150 (MQ=255)

                                                        aCAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCt                                                                                            >  2:1318677/1‑150 (MQ=255)

                                                         cAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGAGGGAGCCGGATTTGAACCGACGACCtt                                                                                           >  2:1482282/1‑150 (MQ=255)

                                                            tgtgtgGTTTGTATTTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGATTGCTTATCAGCAGGCAAAGAATAAGGCCTCTGTGTTTAGTGTGGCG‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                        <  2:1870844/150‑1 (MQ=18)

                                                            tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                        >  1:4543594/1‑150 (MQ=255)

                                                            tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                        <  2:6162033/150‑1 (MQ=255)

                                                              tgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGt                                                                                      >  1:3820289/1‑150 (MQ=255)

                                                              tgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATAAGCAGGCAAAGAAAAAGGCCACTGAGATAACAGTGGCA‑ATGTAGTGAAACTCGTTCACTGAGCCGGATTTGAACAGACGACCTGCGGGt                                                                                      >  1:6644998/1‑150 (MQ=17)

                                                               gtgGTTTGTATGTCAAGCTCTAGGCTTTGATTTTTCGACGTTTTTTCGCGTATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGtt                                                                                     <  1:2355079/150‑1 (MQ=255)

                                                                tgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTa                                                                                    >  1:2095108/1‑150 (MQ=255)

                                                                  gTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGACGGATTTGAACCGACGACCTTCGGGTTAtt                                                                                  >  1:6650651/1‑149 (MQ=255)

                                                                   tttGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGa                                                                                 >  1:6103427/1‑150 (MQ=255)

                                                                     tGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGACGGATTTGAACCTACGACCTTCGGGTTATGAGc                                                                               >  2:2095815/1‑150 (MQ=255)

                                                                      gTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGcc                                                                              >  1:788811/1‑150 (MQ=255)

                                                                      gTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGAGAATGCTTATAAGCAGGCAAAGAAAAAGGCCACTGAGTTAACAGTGGCC‑ATATCTTGACACAGTATGCTGGTGACGGATTTGAACAGACGACCTTCGGGTTATGTGcc                                                                              >  2:936284/1‑150 (MQ=14)

                                                                      gTATGACAAGCGCTAGGCTTTTTTTTTTCGACGTTTTTTCTCGAATGCTTATCAGCAGTCACAGAATAAGGCCACTGTGTTACCAGTTGCG‑TTTTGTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGcc                                                                              <  2:469743/150‑1 (MQ=17)

                                                                       tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCG‑TTATCTTGAATATGGTTGCGGGAGACTGCTTTTAAGAGACGACCTTCGGGTTATGAGccc                                                                             >  2:2810872/1‑150 (MQ=17)

                                                                       tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                             >  1:3875866/1‑150 (MQ=255)

                                                                       tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTGTCGCGAATGCTTATCAGCAGGCACAGAACAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                             <  2:4964052/150‑1 (MQ=255)

                                                                          gTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgac                                                                          >  1:3442922/1‑150 (MQ=255)

                                                                           tCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacg                                                                         >  2:4644193/1‑150 (MQ=255)

                                                                            cAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCATCGGGTTATGAGCCcgacga                                                                        >  1:6279107/1‑150 (MQ=255)

                                                                              cgcgcTCCGCTTTGATTTTACGACGTTATTTCGCGCATGCTTATCCCCAGGCAAAGAATATCGCCACTGTTTTAACTTTGGAC‑TTTTCATGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGc                                                                      <  2:2757551/149‑1 (MQ=17)

                                                                                        tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctg                                                            >  1:915831/1‑150 (MQ=255)

                                                                                         ttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGCCGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctgc                                                           <  1:159752/150‑1 (MQ=255)

                                                                                         ttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgac                                                                          >  2:5421348/1‑135 (MQ=255)

                                                                                         ttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctgc                                                           >  1:251346/1‑150 (MQ=255)

                                                                                           gATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTc                                                         >  1:5925083/1‑150 (MQ=255)

                                                                                              ttttCGTCCTTTTTTCGCGAATGCTTATCAGCAGGCTAAGAAATAGGCAACTGTGTTAACAGGTTTC‑TTATCTTGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCAt                                                      <  2:1980474/150‑1 (MQ=17)

                                                                                                   gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCcgc                                                 >  1:625757/1‑150 (MQ=255)

                                                                                                    aCGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCcgcg                                                >  1:3644118/1‑150 (MQ=255)

                                                                                                     cGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGt                                               >  1:6017460/1‑150 (MQ=255)

                                                                                                              gcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TGTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGc                                      >  2:4095117/1‑150 (MQ=255)

                                                                                                               cgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCg                                     >  1:1160179/1‑150 (MQ=255)

                                                                                                                      ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                   <  1:1468653/97‑1 (MQ=255)

                                                                                                                      ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTa                              >  1:5189018/1‑150 (MQ=255)

                                                                                                                      ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTa                              >  1:2232439/1‑150 (MQ=255)

                                                                                                                          cagcagGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGc                          <  2:5078718/150‑1 (MQ=255)

                                                                                                                           agcagGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCg                         >  1:4217232/1‑150 (MQ=255)

                                                                                                                            gcagGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                   >  2:2952188/1‑91 (MQ=40)

                                                                                                                            gcagGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCgg                        >  1:4908439/1‑149 (MQ=255)

                                                                                                                                gCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCgacgac                                                                                              >  1:2146429/1‑76 (MQ=39)

                                                                                                                                gCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAg                    <  1:3676929/150‑1 (MQ=255)

                                                                                                                                  aaaGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcg                                                                                                   >  2:3756025/1‑69 (MQ=255)

                                                                                                                                  aaaGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacg                                                                         <  2:6793339/95‑1 (MQ=37)

                                                                                                                                  aaaGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgc                                                              <  2:6516776/106‑1 (MQ=35)

                                                                                                                                  aaaGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgc                                                              >  1:6516819/1‑106 (MQ=35)

                                                                                                                                  aaaGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGtt                  >  1:4252495/1‑150 (MQ=255)

                                                                                                                                   aaGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGTATTCTACAGCGTGTAGttt                 >  1:1349756/1‑150 (MQ=255)

                                                                                                                                   aaGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGttt                 >  1:3936371/1‑150 (MQ=255)

                                                                                                                                    aagaaaaGGCCACTGTTAACACAGTGGCC‑TTTTTTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTta                >  1:3694465/4‑150 (MQ=17)

                                                                                                                                    aGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGTATTCTACAGCGTGTAGTTta                <  2:1349988/150‑1 (MQ=255)

                                                                                                                                     gAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTtat               <  1:5337985/150‑1 (MQ=255)

                                                                                                                                     gAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTtat               >  2:851316/1‑150 (MQ=255)

                                                                                                                                      gaaaaGGCCACTGTTAACACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTAGGGTTATGAGCCCCACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGGGTAgggtatt              >  1:5476060/2‑144 (MQ=17)

                                                                                                                                          aGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTc          <  2:6561836/150‑1 (MQ=255)

                                                                                                                                           ggCCACTGTTAACACAGTGGCCTTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGGCCAGCAACCAGGCTGCACAAACCCCCCTCACTGGCGCGGATTCTACAGCCTGAAGGTTATAGGTc          >  1:3481603/1‑150 (MQ=9)

                                                                                                                                           ggCCACTGTTAACACAGTGGCCTTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCAGACGAGCTACCAGCCTGCTCAATCCCGCCACAGTGGGGCGGATTCTACAGCGTGTAGTGTATATGTc          >  1:583628/1‑150 (MQ=17)

                                                                                                                                            gCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCaa        >  1:4788772/1‑150 (MQ=255)

                                                                                                                                              cACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAc                                                                   >  2:6291677/1‑89 (MQ=38)

                                                                                                                                                 tgtgTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAg                                                                                >  2:5630283/1‑73 (MQ=39)

                                                                                                                                                 tgtgTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAg                                                                                <  1:5630283/73‑1 (MQ=39)

                                                                                                                                                 tgtgTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCt                                                                     <  2:4422197/84‑1 (MQ=38)

                                                                                                                                                 tgtgTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCt                                                                     >  1:4422197/1‑84 (MQ=38)

                                                                                                                                                  gtgtTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTa  <  2:6122043/150‑1 (MQ=255)

TTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTA  >  NC_002947/1385529‑1385824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`