BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,388,580	A→C	5.6%	L469R (CTG→CGG) ‡	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,388,580	0	A	C	5.6%	861.4 / 28.1	251	L469R (CTG→CGG) ‡	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base A (129/108); new base C (5/9); total (134/117)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 255 total aligned reads displayed.

ACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTG  >  NC_002947/1388431‑1388729

  ttGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAgg                                                                                                                                                     <  2:4899250/150‑1 (MQ=255)

  ttGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAgg                                                                                                                                                     <  1:5008230/150‑1 (MQ=255)

  ttGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAgg                                                                                                                                                     <  2:4308139/150‑1 (MQ=255)

    gATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGc                                                                                                                                                   <  1:3439889/150‑1 (MQ=255)

           gggCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTc                                                                                                                                            <  1:1083687/150‑1 (MQ=255)

             gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCa                                                                                                                                          <  1:4639729/150‑1 (MQ=255)

             gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCa                                                                                                                                          <  2:5146336/150‑1 (MQ=255)

             gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCa                                                                                                                                          <  2:6866102/150‑1 (MQ=255)

             gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCa                                                                                                                                          <  2:466252/150‑1 (MQ=255)

              cTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAg                                                                                                                                         <  1:6433395/150‑1 (MQ=255)

               tGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAga                                                                                                                                        >  1:4266197/1‑150 (MQ=255)

                 gTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAgatg                                                                                                                                      >  1:903619/1‑150 (MQ=255)

                 gTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAgatg                                                                                                                                      >  1:3284147/1‑150 (MQ=255)

                  ttttCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAgatga                                                                                                                                     >  1:3101193/1‑150 (MQ=255)

                  ttttCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAgatga                                                                                                                                     >  1:4872912/1‑150 (MQ=255)

                    ttCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGatgatg                                                                                                                                   >  1:4557565/1‑150 (MQ=255)

                       ttGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGccc                                                                                                                                <  1:2263748/150‑1 (MQ=255)

                       ttGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGccc                                                                                                                                >  2:1371924/1‑150 (MQ=255)

                       ttGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGccc                                                                                                                                >  1:3129102/1‑150 (MQ=255)

                         gTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTGTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCtt                                                                                                                              >  2:5961943/1‑150 (MQ=255)

                            gcgGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtc                                                                                                                           >  2:535454/1‑150 (MQ=255)

                             cgGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtcg                                                                                                                          >  1:5172807/1‑150 (MQ=255)

                              ggTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtcgt                                                                                                                         <  2:6211006/150‑1 (MQ=255)

                              ggTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtcgt                                                                                                                         <  2:3172611/150‑1 (MQ=255)

                               gtcgtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtcgtc                                                                                                                        >  2:4853525/1‑150 (MQ=255)

                                  gtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATa                                                                                                                     >  1:5243179/1‑150 (MQ=255)

                                      agcagcACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGCCGTCATAGctc                                                                                                                 >  2:2675006/1‑150 (MQ=255)

                                         agcaCTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGc                                                                                                              >  2:208398/1‑150 (MQ=255)

                                           caCTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTc                                                                                                            >  1:2686314/1‑150 (MQ=255)

                                              ttCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGag                                                                                                         >  2:4166661/1‑149 (MQ=255)

                                              ttCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGAt                                                                                                         >  2:4728998/1‑150 (MQ=255)

                                              ttCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGAt                                                                                                         >  1:5956700/1‑150 (MQ=255)

                                                cATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATgg                                                                                                       <  1:5118157/150‑1 (MQ=255)

                                                  tAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCa                                                                                                     <  1:4672401/150‑1 (MQ=255)

                                                  tAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCa                                                                                                     >  1:5594330/1‑150 (MQ=255)

                                                   aGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAg                                                                                                    >  1:5096130/1‑150 (MQ=255)

                                                        gcggcAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCa                                                                                               <  1:3552219/150‑1 (MQ=255)

                                                             agtcagtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACcac                                                                                          >  1:1496755/1‑150 (MQ=255)

                                                               tcagtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCacac                                                                                        <  2:743986/150‑1 (MQ=255)

                                                               tcagtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCacac                                                                                        >  2:871020/1‑150 (MQ=255)

                                                                cagtTCGTCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCTTCATAGCTCTGCTCGATGGCAGCTGCAACCACACg                                                                                       <  1:395739/150‑1 (MQ=255)

                                                                cagtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACg                                                                                       <  1:2380765/150‑1 (MQ=255)

                                                                   ttCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGa                                                                                    >  1:2493601/1‑150 (MQ=255)

                                                                   ttCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGa                                                                                    <  1:2056785/150‑1 (MQ=255)

                                                                   ttCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGAGGGCAGCTGCAACCACACGGGa                                                                                    >  2:6347921/1‑150 (MQ=255)

                                                                        cATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGc                                                                               <  2:3489599/150‑1 (MQ=255)

                                                                        cATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGc                                                                               <  2:4677174/150‑1 (MQ=255)

                                                                          tACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCa                                                                             >  1:6173352/1‑150 (MQ=255)

                                                                           aCAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCaa                                                                            <  2:1390495/150‑1 (MQ=255)

                                                                             aGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATg                                                                          <  2:2381971/150‑1 (MQ=255)

                                                                              gCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGc                                                                         >  2:3545813/1‑150 (MQ=255)

                                                                                  gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                     <  1:1930403/150‑1 (MQ=255)

                                                                                  gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                     <  1:3827534/150‑1 (MQ=255)

                                                                                  gTCGGTTGCTTCGCGGACCACCTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                     <  2:2154554/150‑1 (MQ=255)

                                                                                      gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCaa                                                                 <  2:4020996/150‑1 (MQ=255)

                                                                                      gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCaa                                                                 <  2:1900140/150‑1 (MQ=255)

                                                                                      gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCaa                                                                 <  2:5206080/150‑1 (MQ=255)

                                                                                           ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                            >  1:810013/1‑150 (MQ=255)

                                                                                           ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                            >  1:745671/1‑150 (MQ=255)

                                                                                           ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                            >  1:2634713/1‑150 (MQ=255)

                                                                                           ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                            >  1:4231193/1‑150 (MQ=255)

                                                                                            tCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATc                                                           <  2:4557565/150‑1 (MQ=255)

                                                                                              gcgGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGa                                                         <  1:5507754/150‑1 (MQ=255)

                                                                                                ggACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACa                                                       <  1:6490313/150‑1 (MQ=255)

                                                                                                ggACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACa                                                       <  2:605047/150‑1 (MQ=255)

                                                                                                ggACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACa                                                       <  1:572125/150‑1 (MQ=255)

                                                                                                 gACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAg                                                      <  2:1472172/150‑1 (MQ=255)

                                                                                                   ccACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTa                                                    <  2:1637562/150‑1 (MQ=255)

                                                                                                   ccACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTa                                                    >  2:6588109/1‑150 (MQ=255)

                                                                                                    cACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTAc                                                   >  2:1836165/1‑150 (MQ=255)

                                                                                                     aCGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACg                                                  >  1:6680876/1‑150 (MQ=255)

                                                                                                            tttCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCt                                           >  2:2347710/1‑150 (MQ=255)

                                                                                                            tttCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCt                                           >  1:1577731/1‑150 (MQ=255)

                                                                                                            tttCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCt                                           <  2:5817625/150‑1 (MQ=255)

                                                                                                                gTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGcc                                       <  2:5087343/150‑1 (MQ=255)

                                                                                                                   gcgcAGTGGGACCAGGGCAATCTGGGACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGtt                                    <  2:3659079/150‑1 (MQ=255)

                                                                                                                    cgcAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTtc                                   <  2:5524803/150‑1 (MQ=255)

                                                                                                                    cgcAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTtc                                   <  1:4333529/150‑1 (MQ=255)

                                                                                                                      cAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTtctc                                 <  2:3682029/150‑1 (MQ=255)

                                                                                                                       aGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCg                                >  2:2609015/1‑150 (MQ=255)

                                                                                                                         tGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGcc                              >  2:6284903/1‑150 (MQ=255)

                                                                                                                           ggACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCa                            <  1:4872555/150‑1 (MQ=255)

                                                                                                                            gACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAg                           <  1:6125479/150‑1 (MQ=255)

                                                                                                                             aCCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGc                          >  1:3862152/1‑150 (MQ=255)

                                                                                                                              ccAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCa                         >  2:4809422/1‑150 (MQ=255)

                                                                                                                               cAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCAc                        >  1:1160223/1‑150 (MQ=255)

                                                                                                                                 gggCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCt                      >  2:5868203/1‑150 (MQ=255)

                                                                                                                                 gggCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCt                      >  1:3793131/1‑150 (MQ=255)

                                                                                                                                  ggCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTg                     <  1:1839241/150‑1 (MQ=255)

                                                                                                                                  ggCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTACTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCCCCCAGCACCTg                     >  1:2067503/1‑150 (MQ=255)

                                                                                                                                      aTCTGGAACGACGCCCTGGCTCCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCAc                 >  2:1196139/1‑150 (MQ=255)

                                                                                                                                       tCTGGAACGACGCCCTGGCTCCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACt                >  2:1734265/1‑150 (MQ=255)

                                                                                                                                         tGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTc              >  1:6564790/1‑150 (MQ=255)

                                                                                                                                           gAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACa                                                       >  2:763330/1‑107 (MQ=255)

                                                                                                                                             aCGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAgcg          >  1:6023353/1‑150 (MQ=255)

                                                                                                                                                  gCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCg     >  2:5414091/1‑150 (MQ=255)

                                                                                                                                                    cAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCt   <  2:5447468/150‑1 (MQ=255)

                                                                                                                                                    cAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCt   <  2:3284147/150‑1 (MQ=255)

                                                                                                                                                    cAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCt   <  1:928431/150‑1 (MQ=255)

                                                                                                                                                     aGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTg  >  2:3916856/1‑150 (MQ=255)

ACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTG  >  NC_002947/1388431‑1388729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`