Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,388,585 G→T 5.6% D467E (GAC→GAA) ‡ proS ← proline‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,388,5850GT 5.6% 845.1 / 27.6 249D467E (GAC→GAA) ‡proSproline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (133/102);  new base T (5/9);  total (138/111)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 254 total aligned reads displayed.

TGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTGTACTT  >  NC_002947/1388441‑1388734
                                                                                                                                                  |                                                                                                                                                     
tGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGtt                                                                                                                                                  >  1:2146454/1‑150 (MQ=255)
 gggCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTc                                                                                                                                                 <  1:1083687/150‑1 (MQ=255)
 gggCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTc                                                                                                                                                 <  1:6364847/150‑1 (MQ=255)
  ggCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGACGCCCTGGCTCCGTTcc                                                                                                                                                <  1:2912864/150‑1 (MQ=255)
   gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCa                                                                                                                                               <  1:4639729/150‑1 (MQ=255)
   gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCa                                                                                                                                               <  2:466252/150‑1 (MQ=255)
   gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCa                                                                                                                                               <  2:6866102/150‑1 (MQ=255)
   gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCa                                                                                                                                               <  2:5146335/150‑1 (MQ=255)
    cTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCATCATCTGGAACGACGCCCTGGCTCCGTTcc                                                                                                                                                <  2:5500705/150‑1 (MQ=38)
    cTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAg                                                                                                                                              <  1:6433395/150‑1 (MQ=255)
     tGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACAAGGGCATCATCTGGAACGACGCCCTGGCTCCGTTCCa                                                                                                                                               <  1:6806514/150‑1 (MQ=37)
       gTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAgatg                                                                                                                                           >  1:903619/1‑150 (MQ=255)
        ttttCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAgatga                                                                                                                                          >  1:3101193/1‑150 (MQ=255)
          ttCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGatgatg                                                                                                                                        >  1:4557565/1‑150 (MQ=255)
            cTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCATCATCTGGAACGACGCCCTGGCTCCGTTCCAgattgcc                                                                                                                                        <  2:5588990/150‑5 (MQ=37)
             ttGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGccc                                                                                                                                     >  2:3983701/1‑150 (MQ=255)
             ttGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGccc                                                                                                                                     >  1:3972822/1‑150 (MQ=255)
               gTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCATCATCTGGAACGACGCCCTGGCTCCGTTCCAgattgccctg                                                                                                                                     <  2:4820222/150‑8 (MQ=25)
               gTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTGTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCtt                                                                                                                                   >  2:5961943/1‑150 (MQ=255)
                  gcgGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtc                                                                                                                                >  1:5447468/1‑150 (MQ=255)
                    ggTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtcgt                                                                                                                              <  2:6211006/150‑1 (MQ=255)
                     gtcgtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtcgtc                                                                                                                             >  2:4853525/1‑150 (MQ=255)
                        gtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATa                                                                                                                          >  1:5243179/1‑150 (MQ=255)
                         tcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAg                                                                                                                         <  2:1449709/150‑1 (MQ=255)
                           cagcagCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGct                                                                                                                       <  1:4317565/150‑1 (MQ=255)
                            agcagcACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGCCGTCATAGctc                                                                                                                      >  2:2675006/1‑150 (MQ=255)
                               agcaCTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGc                                                                                                                   >  2:208398/1‑150 (MQ=255)
                               agcaCTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGc                                                                                                                   <  1:5966622/150‑1 (MQ=255)
                               agcaCTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGc                                                                                                                   >  2:592575/1‑150 (MQ=255)
                                 caCTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTc                                                                                                                 <  2:3900514/150‑1 (MQ=255)
                                    ttCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGAt                                                                                                              >  1:1356385/1‑150 (MQ=255)
                                    ttCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGAt                                                                                                              >  2:4728998/1‑150 (MQ=255)
                                      cATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATgg                                                                                                            <  1:5118157/150‑1 (MQ=255)
                                        tAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCa                                                                                                          <  1:4672401/150‑1 (MQ=255)
                                        tAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCa                                                                                                          >  1:5594330/1‑150 (MQ=255)
                                           ccGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCt                                                                                                       >  2:1260936/1‑150 (MQ=255)
                                           ccGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCt                                                                                                       >  2:4302369/1‑150 (MQ=255)
                                              gcggcAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCa                                                                                                    <  1:3552219/150‑1 (MQ=255)
                                                  cagtcagtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACca                                                                                                <  1:208398/150‑1 (MQ=255)
                                                   agtcagtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACcac                                                                                               >  1:1496755/1‑150 (MQ=255)
                                                   agtcagtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACcac                                                                                               >  2:1864702/1‑150 (MQ=255)
                                                     tcagtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCacac                                                                                             <  2:743986/150‑1 (MQ=255)
                                                     tcagtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCacac                                                                                             >  2:871020/1‑150 (MQ=255)
                                                      cagtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACg                                                                                            <  1:2380765/150‑1 (MQ=255)
                                                         ttCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGa                                                                                         <  1:2056785/150‑1 (MQ=255)
                                                         ttCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGa                                                                                         >  1:2493601/1‑150 (MQ=255)
                                                         ttCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGAGGGCAGCTGCAACCACACGGGa                                                                                         >  2:6347921/1‑150 (MQ=255)
                                                               aTACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGcc                                                                                   >  2:5836768/1‑150 (MQ=255)
                                                                 aCAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCaa                                                                                 <  2:1390495/150‑1 (MQ=255)
                                                                   aGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATg                                                                               >  1:4328655/1‑150 (MQ=255)
                                                                   aGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATg                                                                               >  1:4988361/1‑150 (MQ=255)
                                                                    gCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGc                                                                              >  2:3545813/1‑150 (MQ=255)
                                                                        gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACCCGGGACACGCCAATGCCGTa                                                                          <  2:3654623/150‑1 (MQ=255)
                                                                        gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                          <  1:3827534/150‑1 (MQ=255)
                                                                        gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                          <  2:2154155/150‑1 (MQ=255)
                                                                        gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                          <  2:345984/150‑1 (MQ=255)
                                                                         tCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtcgtc                                                                                                                             >  1:5074882/1‑98 (MQ=255)
                                                                            gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCaa                                                                      >  2:2577213/1‑150 (MQ=255)
                                                                            gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCaa                                                                      <  2:5206080/150‑1 (MQ=255)
                                                                                 ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                                 >  1:4231193/1‑150 (MQ=255)
                                                                                 ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                                 >  1:745671/1‑150 (MQ=255)
                                                                                 ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                                 >  1:2634713/1‑150 (MQ=255)
                                                                                  tCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATc                                                                <  2:4557565/150‑1 (MQ=255)
                                                                                  tCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATc                                                                >  1:3155585/1‑150 (MQ=255)
                                                                                     cgGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGAc                                                             <  2:2963011/150‑1 (MQ=255)
                                                                                      ggACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACa                                                            <  2:605047/150‑1 (MQ=255)
                                                                                       gACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAg                                                           >  1:1824370/1‑150 (MQ=255)
                                                                                         ccACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTa                                                         >  2:6588109/1‑150 (MQ=255)
                                                                                         ccACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTa                                                         <  2:1637562/150‑1 (MQ=255)
                                                                                         ccACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTa                                                         <  1:569890/150‑1 (MQ=255)
                                                                                          cACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTAc                                                        >  2:1836165/1‑150 (MQ=255)
                                                                                           aCGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACg                                                       >  1:882438/1‑150 (MQ=255)
                                                                                             gTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGAc                                                     >  1:3353221/1‑150 (MQ=255)
                                                                                                      gTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGcc                                            <  2:5087343/150‑1 (MQ=255)
                                                                                                        aGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGt                                          >  1:6557352/1‑150 (MQ=255)
                                                                                                          cgcAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTtc                                        <  2:4143089/150‑1 (MQ=255)
                                                                                                            cAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTtctc                                      <  2:3682029/150‑1 (MQ=255)
                                                                                                            cAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTtctc                                      >  2:2379981/1‑150 (MQ=255)
                                                                                                               tGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGcc                                   >  2:6284903/1‑150 (MQ=255)
                                                                                                               tGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGcc                                   >  2:1707310/1‑150 (MQ=255)
                                                                                                                 ggACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCa                                 <  1:4872597/150‑1 (MQ=255)
                                                                                                                  gACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAg                                <  1:2018436/150‑1 (MQ=255)
                                                                                                                  gACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAg                                <  1:6125479/150‑1 (MQ=255)
                                                                                                                   aCCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGc                               >  1:3862152/1‑150 (MQ=255)
                                                                                                                      aGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCAcc                            >  2:5336354/1‑150 (MQ=255)
                                                                                                                       gggCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCt                           >  1:3793131/1‑150 (MQ=255)
                                                                                                                        ggCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTg                          <  1:1839241/150‑1 (MQ=255)
                                                                                                                        ggCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTg                          >  2:2241418/1‑150 (MQ=255)
                                                                                                                              aTCTGGAACGACGCCCTGGCTCCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCgg                                                  >  1:5161895/1‑122 (MQ=38)
                                                                                                                              aTCTGGAACGACGCCCTGGCTCCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCAc                      >  2:1196139/1‑150 (MQ=255)
                                                                                                                                 tGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTc                   >  1:6564790/1‑150 (MQ=255)
                                                                                                                                 tGGAACGACGCCCTGGCTCCGTTCCAGAT‑‑TGCCCTGGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAg                 >  2:4686798/1‑150 (MQ=38)
                                                                                                                                   gAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACa                                                            >  2:763330/1‑107 (MQ=255)
                                                                                                                                     aCGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAgcg               >  1:6023385/1‑150 (MQ=255)
                                                                                                                                          gCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCg          >  2:5414091/1‑150 (MQ=255)
                                                                                                                                            cAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCt        <  2:3284147/150‑1 (MQ=255)
                                                                                                                                              gggCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTGt      >  2:1013012/1‑150 (MQ=255)
                                                                                                                                                gCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTGTAc    <  2:5434492/150‑1 (MQ=255)
                                                                                                                                                 cgtcgtTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTGTACt   <  1:4853525/150‑1 (MQ=255)
                                                                                                                                                  gtcgtTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTGTACtt  <  1:1059684/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTGTACTT  >  NC_002947/1388441‑1388734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑