BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,429,207	A→C	5.8%	E321A (GAG→GCG) ‡	PP_5467 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,429,207	0	A	C	5.8%	978.3 / 33.6	292	E321A (GAG→GCG) ‡	PP_5467	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (152/121); new base C (6/11); total (158/134)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 308 total aligned reads displayed.

GCTCAAGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTCG  >  NC_002947/1429058‑1429356

gCTCAAGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAaga                                                                                                                                                       <  1:3146716/150‑1 (MQ=255)

    aaGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCt                                                                                                                                                   >  2:638286/1‑150 (MQ=255)

    aaGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCt                                                                                                                                                   >  2:4378678/1‑150 (MQ=255)

        gTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCa                                                                                                                                               <  1:5958121/150‑1 (MQ=255)

        gTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCa                                                                                                                                               <  1:2259666/150‑1 (MQ=255)

         tGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAg                                                                                                                                              <  1:5067403/150‑1 (MQ=255)

         ggCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAg                                                                                                                                              <  2:2260536/149‑1 (MQ=255)

          gCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGc                                                                                                                                             <  1:3851534/150‑1 (MQ=255)

          gCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGc                                                                                                                                             <  1:2841342/150‑1 (MQ=255)

          gCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGc                                                                                                                                             <  2:449351/150‑1 (MQ=255)

            ccTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGCGa                                                                                                                                           <  1:3360041/150‑1 (MQ=255)

            ccTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGCGa                                                                                                                                           <  2:4025358/150‑1 (MQ=255)

             cTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGaa                                                                                                                                          <  1:372201/150‑1 (MQ=255)

              tGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAAt                                                                                                                                         >  1:5533797/1‑150 (MQ=255)

              tGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAAt                                                                                                                                         >  2:3642287/1‑150 (MQ=255)

                  cgcgATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACca                                                                                                                                     <  2:2754322/150‑1 (MQ=255)

                    cgATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGTCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACcagc                                                                                                                                   <  2:2726734/150‑1 (MQ=255)

                     gATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACcagca                                                                                                                                  <  2:1213338/150‑1 (MQ=255)

                             ttGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTcc                                                                                                                          >  2:1608223/1‑150 (MQ=255)

                                gcgcGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCa                                                                                                                       >  2:3675672/1‑150 (MQ=255)

                                gcgcGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCa                                                                                                                       >  1:1252946/1‑150 (MQ=255)

                                  gcgTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAg                                                                                                                     >  2:343370/1‑150 (MQ=255)

                                  gcgTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAg                                                                                                                     >  2:320059/1‑150 (MQ=255)

                                    gTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGc                                                                                                                   >  1:4833259/1‑150 (MQ=255)

                                      aCAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCaa                                                                                                                 >  2:757435/1‑150 (MQ=255)

                                       cAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCaaa                                                                                                                <  1:1087175/150‑1 (MQ=255)

                                       cAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCaaa                                                                                                                <  2:3323979/150‑1 (MQ=255)

                                       cAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCaaa                                                                                                                <  2:6785440/150‑1 (MQ=255)

                                        aGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAg                                                                                                               >  1:4225253/1‑150 (MQ=255)

                                          cccGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGGGCGGCTGTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCGTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCACAGtt                                                                                                             >  1:1519666/1‑150 (MQ=255)

                                          cccGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGtt                                                                                                             >  2:2841607/1‑150 (MQ=255)

                                          cccGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGtt                                                                                                             >  2:4771190/1‑150 (MQ=255)

                                             gTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGc                                                                                                          >  2:1325316/1‑150 (MQ=255)

                                             gTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGc                                                                                                          <  1:6829045/150‑1 (MQ=255)

                                              tACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCa                                                                                                         <  1:4800658/150‑1 (MQ=255)

                                              tACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCa                                                                                                         <  1:1851717/150‑1 (MQ=255)

                                               acacCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAg                                                                                                        >  2:1878531/1‑150 (MQ=255)

                                                 acCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCACAGTTCGCAGcc                                                                                                      >  2:5485651/1‑150 (MQ=255)

                                                 acCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGcc                                                                                                      >  2:4597316/1‑150 (MQ=255)

                                                   cGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCtt                                                                                                    >  2:1162937/1‑150 (MQ=255)

                                                     gCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCt                                                                                                  >  1:6465245/1‑150 (MQ=255)

                                                         ggCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTTCAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCa                                                                                              <  1:1928622/150‑1 (MQ=255)

                                                                ttGGCTGAAGCGGTGAGCGGCTTTGCCCGCGAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGtt                                                                                       >  1:5129640/1‑150 (MQ=255)

                                                                 tGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCaa                                                                                                                      <  1:5390075/118‑1 (MQ=255)

                                                                  ggCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTgg                                                                                     <  2:6678329/150‑1 (MQ=255)

                                                                   gCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTggg                                                                                    >  1:5605298/1‑150 (MQ=255)

                                                                     tGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCt                                                                                  <  2:6017616/150‑1 (MQ=255)

                                                                        aGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTgtc                                                                               <  1:4130240/150‑1 (MQ=255)

                                                                             tGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTcgtcgt                                                                          <  1:6117388/150‑1 (MQ=255)

                                                                              gAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTg                                                                         <  2:495701/150‑1 (MQ=255)

                                                                               aGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTgg                                                                        >  1:460271/1‑150 (MQ=255)

                                                                                gCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGa                                                                       <  1:3473050/150‑1 (MQ=255)

                                                                                gCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGa                                                                       >  1:894442/1‑150 (MQ=255)

                                                                                gCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGa                                                                       <  2:693840/150‑1 (MQ=255)

                                                                                 cGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAg                                                                      <  1:1000761/150‑1 (MQ=255)

                                                                                     tttGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGt                                                                  <  1:2608000/150‑1 (MQ=255)

                                                                                     tttGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGt                                                                  >  2:2092710/1‑150 (MQ=255)

                                                                                          ccGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACtt                                                             >  1:236496/1‑150 (MQ=255)

                                                                                          ccGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACtt                                                             >  2:2415681/1‑150 (MQ=255)

                                                                                              caGGCCAAGGTCGAGAACTACTCGGGGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGgc                                                         <  2:5879670/149‑1 (MQ=255)

                                                                                                  ccAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgcca                                                     >  1:3000324/1‑150 (MQ=255)

                                                                                                   cAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaa                                                    <  2:3833903/150‑1 (MQ=255)

                                                                                                   cAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaa                                                    <  1:5229101/150‑1 (MQ=255)

                                                                                                   cAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaa                                                    <  2:1711390/150‑1 (MQ=255)

                                                                                                    aaGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaag                                                   >  2:4149553/1‑150 (MQ=255)

                                                                                                       gTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaaggcc                                                <  2:5170729/150‑1 (MQ=255)

                                                                                                        tCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaaggcca                                               >  2:1969232/1‑150 (MQ=255)

                                                                                                         cGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaaggccaa                                              <  2:2533399/150‑1 (MQ=255)

                                                                                                           agaACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGa                                            >  1:685979/1‑150 (MQ=255)

                                                                                                             aaCTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAAc                                          <  2:5532446/150‑1 (MQ=255)

                                                                                                                tactCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACcac                                       >  2:4618611/1‑150 (MQ=255)

                                                                                                                 tctCGGTTAGCTAGAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGATTACCAGCAGCAAGTCCCCAAGGCAATGTTCGCAGCCTTCTGCCAGGAGGTTTGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGTCCAAGAACcaca                                      <  2:6746169/149‑1 (MQ=255)

                                                                                                                  ctCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAg                                     <  2:6230478/150‑1 (MQ=255)

                                                                                                                  ctCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAg                                     <  1:4872392/150‑1 (MQ=255)

                                                                                                                  ctCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAg                                     >  1:6646004/1‑150 (MQ=255)

                                                                                                                     ggTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCagg                                                                                            >  2:2905714/1‑92 (MQ=255)

                                                                                                                     ggTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCagg                                                                                            <  1:2905714/92‑1 (MQ=255)

                                                                                                                     ggTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGa                                  >  1:3909988/1‑150 (MQ=255)

                                                                                                                           cTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGa                            >  2:1531195/1‑150 (MQ=255)

                                                                                                                               aggggCTTAAAATCGCTGAATGTGGCTTTCAGCGAATACCAGCAGAAAGTCCCCAAGGAAAAGTTCGCAGCATTCTGCCCGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCAAAGAACCACAGCGACTCGGAATgc                        <  1:1698701/147‑1 (MQ=255)

                                                                                                                                gCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATgcg                       >  1:4045277/1‑150 (MQ=255)

                                                                                                                                     ttCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATc                  >  1:6505787/1‑150 (MQ=255)

                                                                                                                                     ttCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATc                  >  1:5809642/1‑150 (MQ=255)

                                                                                                                                       cAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCgg                <  1:1444211/150‑1 (MQ=255)

                                                                                                                                         attCGCTGAAAGCCTCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGc              >  1:2047073/3‑150 (MQ=255)

                                                                                                                                         attCGCTGAAAGCCTCTTTCAGCAAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGc              >  2:844971/3‑150 (MQ=255)

                                                                                                                                         aaTCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGc              <  2:2656380/150‑1 (MQ=255)

                                                                                                                                             gCTGAATGAGGCTTTCAGCGAATACCCGCAGCAAGCCCCCACGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCTGTGACTTTGGCGCCAAGGCCAATAACCACAGCGACTCGGAATGCGCCATCGGGCAGaa          <  1:638286/150‑1 (MQ=255)

                                                                                                                                             gCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGaa          >  2:6282971/1‑150 (MQ=255)

                                                                                                                                               tGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGc        >  2:137461/1‑150 (MQ=255)

                                                                                                                                                gAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGACTGCGCCATCGGGCAGAAGCt       >  2:5020523/1‑150 (MQ=255)

                                                                                                                                                 aaaGAGGCTTTCAGCGAATACCAGCAGCATGCCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGGCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTg      <  1:3028230/150‑1 (MQ=255)

                                                                                                                                                 aaaGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGTTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTg      <  2:6592087/150‑1 (MQ=255)

                                                                                                                                                 aaaGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTg      >  1:6035023/1‑150 (MQ=255)

                                                                                                                                                   agagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGt    <  1:2724418/150‑1 (MQ=255)

                                                                                                                                                   agagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGt    >  2:1767514/1‑150 (MQ=255)

                                                                                                                                                   agagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGt    <  1:1665743/150‑1 (MQ=255)

                                                                                                                                                    gagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCTGGAGGTTGGGCTGTCGTCGTGGAGCATTGACTTTGGCGCCAAGGCCAATAACCACCGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTc   <  1:178480/150‑1 (MQ=255)

                                                                                                                                                    gagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTc   <  2:6109074/150‑1 (MQ=255)

                                                                                                                                                     agGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTCg  >  1:5695848/1‑150 (MQ=255)

GCTCAAGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTCG  >  NC_002947/1429058‑1429356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`