BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,429,208	G→C	5.8%	E321D (GAG→GAC) ‡	PP_5467 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,429,208	0	G	C	5.8%	978.6 / 34.3	290	E321D (GAG→GAC) ‡	PP_5467	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (150/122); new base C (6/11); total (157/133)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

CTCAAGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTCG  >  NC_002947/1429059‑1429356

 tCAAGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAgg                                                                                                                                                     <  1:2209512/150‑1 (MQ=255)

   aaGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCt                                                                                                                                                   >  2:638286/1‑150 (MQ=255)

   aaGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCt                                                                                                                                                   >  2:4378678/1‑150 (MQ=255)

    agagTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCtt                                                                                                                                                  >  1:2282862/1‑150 (MQ=255)

     gagTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCttt                                                                                                                                                 <  1:6093091/150‑1 (MQ=255)

     gagTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCttt                                                                                                                                                 >  2:3662406/1‑150 (MQ=255)

        tGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAg                                                                                                                                              <  1:5067264/150‑1 (MQ=255)

        ggCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAg                                                                                                                                              <  2:2260536/149‑1 (MQ=255)

         gCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGc                                                                                                                                             <  1:183261/150‑1 (MQ=255)

         gCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGc                                                                                                                                             <  1:1600721/150‑1 (MQ=255)

         gCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGc                                                                                                                                             <  1:5678803/150‑1 (MQ=255)

         gCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGc                                                                                                                                             <  2:449351/150‑1 (MQ=255)

         gCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGc                                                                                                                                             <  1:3851534/150‑1 (MQ=255)

         gCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGc                                                                                                                                             <  1:2841342/150‑1 (MQ=255)

           ccTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGCGa                                                                                                                                           <  1:3360041/150‑1 (MQ=255)

           ccTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGCGa                                                                                                                                           <  2:4025358/150‑1 (MQ=255)

            cTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGaa                                                                                                                                          <  1:372201/150‑1 (MQ=255)

             tGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAAt                                                                                                                                         >  1:5533797/1‑150 (MQ=255)

                 cgcgATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACca                                                                                                                                     <  2:2754322/150‑1 (MQ=255)

                  gcgATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACcag                                                                                                                                    <  1:4113021/150‑1 (MQ=255)

                   cgATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGTCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACcagc                                                                                                                                   <  2:2726734/150‑1 (MQ=255)

                    gATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACcagca                                                                                                                                  <  2:1213338/150‑1 (MQ=255)

                      tGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCagcagc                                                                                                                                <  1:5764260/150‑1 (MQ=255)

                            ttGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTcc                                                                                                                          >  2:1608223/1‑150 (MQ=255)

                                     aCAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCaa                                                                                                                 <  1:4842143/150‑1 (MQ=255)

                                     aCAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCaa                                                                                                                 >  2:757435/1‑150 (MQ=255)

                                      cAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCaaa                                                                                                                >  1:6022443/1‑150 (MQ=255)

                                      cAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCaaa                                                                                                                <  1:1087175/150‑1 (MQ=255)

                                      cAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCaaa                                                                                                                <  2:6785440/150‑1 (MQ=255)

                                         cccGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGtt                                                                                                             >  2:4771190/1‑150 (MQ=255)

                                            gTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGc                                                                                                          >  2:1325316/1‑150 (MQ=255)

                                             tACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCa                                                                                                         <  1:4800655/150‑1 (MQ=255)

                                              acacCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAg                                                                                                        >  2:1878531/1‑150 (MQ=255)

                                                acCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTc                                                                                                                           <  1:6848041/129‑1 (MQ=255)

                                                acCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGcc                                                                                                      <  2:2532354/150‑1 (MQ=255)

                                                acCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGcc                                                                                                      >  2:4597316/1‑150 (MQ=255)

                                                  cGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCtt                                                                                                    >  2:1162937/1‑150 (MQ=255)

                                                          cGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCagg                                                                                            <  1:4828476/150‑1 (MQ=255)

                                                                tGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCaa                                                                                                                      <  1:5390075/118‑1 (MQ=255)

                                                                tGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCaa                                                                                                                      >  2:5390075/1‑118 (MQ=255)

                                                                 ggCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTgg                                                                                     <  2:2614291/150‑1 (MQ=255)

                                                                 ggCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTgg                                                                                     <  1:1479290/150‑1 (MQ=255)

                                                                 ggCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTgg                                                                                     <  2:6678329/150‑1 (MQ=255)

                                                                  gCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTggg                                                                                    >  1:5605298/1‑150 (MQ=255)

                                                                    tGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCt                                                                                  <  1:5858965/150‑1 (MQ=255)

                                                                    tGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCt                                                                                  <  2:3797514/150‑1 (MQ=255)

                                                                    tGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCt                                                                                  <  2:6017616/150‑1 (MQ=255)

                                                                      aaGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTgt                                                                                <  1:4014501/150‑1 (MQ=255)

                                                                           gTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGtcgtcg                                                                           >  2:5698458/1‑150 (MQ=255)

                                                                             gAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTg                                                                         <  2:495701/150‑1 (MQ=255)

                                                                               gCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGa                                                                       >  1:5099181/1‑150 (MQ=255)

                                                                               gCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGa                                                                       <  2:693840/150‑1 (MQ=255)

                                                                               gCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGa                                                                       <  1:3473050/150‑1 (MQ=255)

                                                                                cGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAg                                                                      <  1:1000761/150‑1 (MQ=255)

                                                                                  gCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCa                                                                    <  1:1918332/150‑1 (MQ=255)

                                                                                    tttGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGt                                                                  <  1:2608000/150‑1 (MQ=255)

                                                                                    tttGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGt                                                                  >  2:2092710/1‑150 (MQ=255)

                                                                                      tGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGa                                                                <  1:3116344/150‑1 (MQ=255)

                                                                                         ccGCACGGCCAAGGTCGACAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACtt                                                             >  2:5496532/1‑150 (MQ=255)

                                                                                         ccGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACtt                                                             >  2:2415681/1‑150 (MQ=255)

                                                                                         ccGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACtt                                                             >  2:5974327/1‑150 (MQ=255)

                                                                                         ccGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACtt                                                             >  1:4508966/1‑150 (MQ=255)

                                                                                          cGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACttt                                                            <  2:3301567/150‑1 (MQ=255)

                                                                                                  cAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGATGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaa                                                    >  1:3009987/1‑150 (MQ=255)

                                                                                                  cAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaa                                                    <  2:1711390/150‑1 (MQ=255)

                                                                                                  cAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaa                                                    >  1:6498432/1‑150 (MQ=255)

                                                                                                  cAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaa                                                    <  1:5229101/150‑1 (MQ=255)

                                                                                                   aaGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaag                                                   >  2:4149553/1‑150 (MQ=255)

                                                                                                    aGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaagg                                                  <  2:3874316/150‑1 (MQ=255)

                                                                                                       tCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaaggcca                                               >  2:1969232/1‑150 (MQ=255)

                                                                                                         gagaACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaaggccaag                                             >  1:1974800/1‑150 (MQ=255)

                                                                                                               tactCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACcac                                       >  2:4618611/1‑150 (MQ=255)

                                                                                                                tctCGGTTAGCTAGAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGATTACCAGCAGCAAGTCCCCAAGGCAATGTTCGCAGCCTTCTGCCAGGAGGTTTGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGTCCAAGAACcaca                                      <  2:6746169/149‑1 (MQ=255)

                                                                                                                 ctCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAg                                     >  1:6645976/1‑150 (MQ=255)

                                                                                                                 ctCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAg                                     <  2:6230478/150‑1 (MQ=255)

                                                                                                                    ggTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGa                                  >  1:3909988/1‑150 (MQ=255)

                                                                                                                      tGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACt                                >  1:2925848/1‑150 (MQ=255)

                                                                                                                          cTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGa                            >  2:1531195/1‑150 (MQ=255)

                                                                                                                           tACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGaa                           <  2:3466037/150‑1 (MQ=255)

                                                                                                                            aCAGCGGCTTCAAATCGCTGAAAGTGGCTTTCAGCTAATACCAGCAGAAAGTCCCCAAGGCAAAGTTCGCAGCATTCTGCCTGGTGGTTGGGCTGTCGTCGTGGAGCTGTGACTTTGGCGCCAAGGCAAAGAACCACAGCGACTCGGAAt                          <  1:2412075/150‑1 (MQ=255)

                                                                                                                                 ggCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGcc                     <  2:3390824/150‑1 (MQ=255)

                                                                                                                                     tCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCg                 >  1:1308214/1‑150 (MQ=255)

                                                                                                                                        attCGCTGAAAGCCTCTTTCAGCAAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGc              >  2:844971/3‑150 (MQ=255)

                                                                                                                                        attCGCTGAAAGCCTCTTTCAGCAAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGc              >  2:2943131/3‑150 (MQ=255)

                                                                                                                                        aaTCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCGAGTCCCCAAGGCAAAGTTCGCAGCCTTCAGCCAGGAGGTTGGGCTGTCGTCGAGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGc              >  1:4218033/1‑150 (MQ=255)

                                                                                                                                         aTCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGCCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCa             >  1:6243692/1‑150 (MQ=255)

                                                                                                                                            gCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGaa          >  2:6282971/1‑150 (MQ=255)

                                                                                                                                             cTGAAAGCCTCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAg         >  2:2861959/1‑150 (MQ=255)

                                                                                                                                              tGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGc        >  2:137461/1‑150 (MQ=255)

                                                                                                                                              tGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGc        >  1:1736959/1‑150 (MQ=255)

                                                                                                                                               gAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGACTGCGCCATCGGGCAGAAGCt       >  2:5020523/1‑150 (MQ=255)

                                                                                                                                               gAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCggg               >  1:867651/1‑142 (MQ=255)

                                                                                                                                                aaaGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGTTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTg      <  2:6592087/150‑1 (MQ=255)

                                                                                                                                                 aaGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTgg     <  1:6747895/150‑1 (MQ=255)

                                                                                                                                                  agagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGt    <  1:2724418/150‑1 (MQ=255)

                                                                                                                                                  agagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGt    >  2:1767514/1‑150 (MQ=255)

                                                                                                                                                  agagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGt    <  1:1665774/150‑1 (MQ=255)

                                                                                                                                                   gcggCTTCCAGCGAATCCCACCAGCAAGCCCCCACGGCAACGTTCGCACCCTCCTGCCAGGTGTTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTc   <  1:1192293/148‑1 (MQ=255)

                                                                                                                                                   gagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTc   <  2:6109074/150‑1 (MQ=255)

                                                                                                                                                   gagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTc   <  1:6457831/150‑1 (MQ=255)

CTCAAGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTCG  >  NC_002947/1429059‑1429356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`