Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,429,209 G→T 5.7% A322S (GCT→TCT)  PP_5467 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,429,2090GT 5.7% 1015.2 / 34.7 300A322S (GCT→TCT) PP_5467hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (153/130);  new base T (6/11);  total (159/141)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

Only 100 of 312 total aligned reads displayed.

TCAAGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTCGAA  >  NC_002947/1429060‑1429358
                                                                                                                                                     |                                                                                                                                                     
tCAAGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAgg                                                                                                                                                       <  1:2209512/150‑1 (MQ=255)
  aaGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCt                                                                                                                                                     >  2:1698701/1‑150 (MQ=255)
  aaGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCt                                                                                                                                                     >  2:4378678/1‑150 (MQ=255)
   agagTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCtt                                                                                                                                                    >  1:2282862/1‑150 (MQ=255)
    gagTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCttt                                                                                                                                                   <  1:6093091/150‑1 (MQ=255)
    gagTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCttt                                                                                                                                                   >  2:3662406/1‑150 (MQ=255)
       tGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAg                                                                                                                                                <  1:5067403/150‑1 (MQ=255)
       ggCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAg                                                                                                                                                <  2:2260536/149‑1 (MQ=255)
        gCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGc                                                                                                                                               <  1:1600721/150‑1 (MQ=255)
        gCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGc                                                                                                                                               <  2:449351/150‑1 (MQ=255)
          ccTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGCGa                                                                                                                                             <  2:4025358/150‑1 (MQ=255)
          ccTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGCGa                                                                                                                                             <  1:3360041/150‑1 (MQ=255)
          ccTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGCGa                                                                                                                                             <  2:6694767/150‑1 (MQ=255)
                cgcgATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACca                                                                                                                                       <  1:3862331/150‑1 (MQ=255)
                cgcgATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACca                                                                                                                                       <  2:2754322/150‑1 (MQ=255)
                  cgATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGTCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACcagc                                                                                                                                     <  2:2726734/150‑1 (MQ=255)
                  cgATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACcagc                                                                                                                                     <  1:4446619/150‑1 (MQ=255)
                     tGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCagcagc                                                                                                                                  <  1:5759555/150‑1 (MQ=255)
                        tGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAg                                                                                                                               >  2:716665/1‑150 (MQ=255)
                         gATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGt                                                                                                                              >  1:407193/1‑150 (MQ=255)
                                 cgTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAgg                                                                                                                      <  2:1212025/150‑1 (MQ=255)
                                  gTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGc                                                                                                                     >  1:4833259/1‑150 (MQ=255)
                                     cAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCaaa                                                                                                                  <  1:1087175/150‑1 (MQ=255)
                                      aGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAg                                                                                                                 >  1:6396588/1‑150 (MQ=255)
                                      aGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAg                                                                                                                 >  1:56547/1‑150 (MQ=255)
                                       gCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGt                                                                                                                >  2:664203/1‑150 (MQ=255)
                                        cccGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGtt                                                                                                               >  2:4771190/1‑150 (MQ=255)
                                          cGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCg                                                                                                             >  1:4178181/1‑150 (MQ=255)
                                            tACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCa                                                                                                           <  1:4800658/150‑1 (MQ=255)
                                            tACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCa                                                                                                           >  2:1266809/1‑150 (MQ=255)
                                               acCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTc                                                                                                                             >  2:6848041/1‑129 (MQ=255)
                                               acCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGcc                                                                                                        <  2:2532354/150‑1 (MQ=255)
                                               acCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGcc                                                                                                        >  2:4597316/1‑150 (MQ=255)
                                                ccGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCt                                                                                                       >  2:1449067/1‑150 (MQ=255)
                                                   gCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCt                                                                                                    <  2:158993/150‑1 (MQ=255)
                                                              ttGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGtt                                                                                         >  1:6646483/1‑150 (MQ=255)
                                                               tGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCaa                                                                                                                        >  2:5390075/1‑118 (MQ=255)
                                                                ggCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTgg                                                                                       <  1:1479290/150‑1 (MQ=255)
                                                                ggCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTgg                                                                                       <  2:2614291/150‑1 (MQ=255)
                                                                 gCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTggg                                                                                      <  1:3789056/150‑1 (MQ=255)
                                                                  cTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGc                                                                                     <  1:707764/150‑1 (MQ=255)
                                                                   tGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCt                                                                                    <  2:6017616/150‑1 (MQ=255)
                                                                   tGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCt                                                                                    <  2:3797514/150‑1 (MQ=255)
                                                                        cGGTGAGCGGCTCTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTgtcgt                                                                               >  2:1808249/1‑150 (MQ=255)
                                                                          gTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGtcgtcg                                                                             >  2:5698458/1‑150 (MQ=255)
                                                                            gAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTg                                                                           <  2:495701/150‑1 (MQ=255)
                                                                             aGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTgg                                                                          >  1:460271/1‑150 (MQ=255)
                                                                              gCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGa                                                                         <  1:3473050/150‑1 (MQ=255)
                                                                               cGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAg                                                                        <  1:1000761/150‑1 (MQ=255)
                                                                                 gCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCa                                                                      <  1:1918332/150‑1 (MQ=255)
                                                                                   tttGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGt                                                                    <  1:2608000/150‑1 (MQ=255)
                                                                                   tttGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGt                                                                    >  2:2092710/1‑150 (MQ=255)
                                                                                     tGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGa                                                                  <  1:5499551/150‑1 (MQ=255)
                                                                                       cccGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACt                                                                >  2:6408372/1‑150 (MQ=255)
                                                                                        ccGCACGGCCAAGGTCGACAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACtt                                                               >  2:5496532/1‑150 (MQ=255)
                                                                                        ccGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACtt                                                               >  2:5974327/1‑150 (MQ=255)
                                                                                        ccGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACtt                                                               >  2:2415681/1‑150 (MQ=255)
                                                                                         cGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACttt                                                              <  1:5958491/150‑1 (MQ=255)
                                                                                         cGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACttt                                                              <  1:3970996/150‑1 (MQ=255)
                                                                                         cGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACttt                                                              <  2:3301567/150‑1 (MQ=255)
                                                                                              ggCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGgcgc                                                         <  1:5586540/150‑1 (MQ=255)
                                                                                                ccAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgcca                                                       >  1:3000324/1‑150 (MQ=255)
                                                                                                 cAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaa                                                      <  2:3833903/150‑1 (MQ=255)
                                                                                                  aaGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaag                                                     >  2:4149553/1‑150 (MQ=255)
                                                                                                      tCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaaggcca                                                 >  2:1969232/1‑150 (MQ=255)
                                                                                                        gagaACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaaggccaag                                               >  1:1974800/1‑150 (MQ=255)
                                                                                                        gagaACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCACAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaaggccaag                                               >  1:6550935/1‑150 (MQ=255)
                                                                                                              tactCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACcac                                         >  2:4618611/1‑150 (MQ=255)
                                                                                                                ctCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAg                                       <  1:4872392/150‑1 (MQ=255)
                                                                                                                ctCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAg                                       <  2:6230478/150‑1 (MQ=255)
                                                                                                                   ggTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCagg                                                                                              <  1:2905714/92‑1 (MQ=255)
                                                                                                                          tACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGaa                             <  2:3466037/150‑1 (MQ=255)
                                                                                                                          tACAGCGGCTTCAAATCGCTGAAACAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTAGGCGCCAAGGCCAAGAACCACAGCGACTCGGaa                             >  1:5852382/1‑150 (MQ=255)
                                                                                                                           aCAGCGGCTTCAAATCGCTGAAAGTGGCTTTCAGCTAATACCAGCAGAAAGTCCCCAAGGCAAAGTTCGCAGCATTCTGCCTGGTGGTTGGGCTGTCGTCGTGGAGCTGTGACTTTGGCGCCAAGGCAAAGAACCACAGCGACTCGGAAt                            <  1:2412075/150‑1 (MQ=255)
                                                                                                                             aggggCTTAAAATCGCTGAATGTGGCTTTCAGCGAATACCAGCAGAAAGTCCCCAAGGAAAAGTTCGCAGCATTCTGCCCGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCAAAGAACCACAGCGACTCGGAATgc                          <  1:1698701/147‑1 (MQ=255)
                                                                                                                             aGCGGCTTAAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGCGGTTGGGCTGTCGTCGTGGAGCTGTTACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATgc                          <  1:6474520/150‑1 (MQ=255)
                                                                                                                                ggCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGcc                       <  2:3390824/150‑1 (MQ=255)
                                                                                                                                  cTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCAt                     >  1:6842882/1‑150 (MQ=255)
                                                                                                                                   ttCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATc                    >  2:6747895/1‑150 (MQ=255)
                                                                                                                                    tCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCg                   >  1:1308214/1‑150 (MQ=255)
                                                                                                                                       attCGCTGAAAGCCTCTTTCAGCAAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGc                >  2:2943131/3‑150 (MQ=255)
                                                                                                                                        aTCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTTTCGTCTTGGTGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCa               <  2:822710/150‑1 (MQ=255)
                                                                                                                                        aTCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCa               >  2:1137120/1‑150 (MQ=255)
                                                                                                                                        aTCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCa               >  2:1349748/1‑150 (MQ=255)
                                                                                                                                          cGCTGAAAGAGGCTTTCAGCGAATCCCCGCCGCACGTCCCCACGGCAAGGTTCGCAGCGTTCTGCCTGGAGGTGGGGCTGTCGTCGTGGAGCTGTGACTTGGGCGCCACGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGa             <  1:5237515/150‑1 (MQ=255)
                                                                                                                                           gCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGaa            >  2:6282971/1‑150 (MQ=255)
                                                                                                                                            cTGAAAGCCTCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAg           >  2:2861959/1‑150 (MQ=255)
                                                                                                                                              gAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGACTGCGCCATCGGGCAGAAGCt         >  2:5020523/1‑150 (MQ=255)
                                                                                                                                               aaaGAGGCTTTCAGCGAATACCAGCAGCATGCCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGGCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTg        <  1:3028230/150‑1 (MQ=255)
                                                                                                                                               aaaGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGTGGTTGGGCGGTCGTCGTGGAGCTGTGACTTTGGCGCCACGGCCAAGACCCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTg        <  1:5193739/150‑1 (MQ=255)
                                                                                                                                               aaaGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTg        <  2:1736959/150‑1 (MQ=255)
                                                                                                                                                 agagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGt      <  1:2724418/150‑1 (MQ=255)
                                                                                                                                                 agagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGt      <  1:1665743/150‑1 (MQ=255)
                                                                                                                                                  gcggCTTCCAGCGAATCCCACCAGCAAGCCCCCACGGCAACGTTCGCACCCTCCTGCCAGGTGTTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTc     <  1:1192293/148‑1 (MQ=255)
                                                                                                                                                  gagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCTGGAGGTTGGGCTGTCGTCGTGGAGCATTGACTTTGGCGCCAAGGCCAATAACCACCGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTc     <  1:178480/150‑1 (MQ=255)
                                                                                                                                                  gagGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTc     <  2:6109074/150‑1 (MQ=255)
                                                                                                                                                     gCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAACGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTCGaa  <  1:280789/150‑1 (MQ=255)
                                                                                                                                                     gCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTCGaa  >  1:6239163/1‑150 (MQ=255)
                                                                                                                                                     gCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTCGaa  <  1:5084975/150‑1 (MQ=255)
                                                                                                                                                     gCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTCGaa  >  2:855684/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCAAGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGTCGAA  >  NC_002947/1429060‑1429358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑