BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,756,071	T→A	5.2%	S147T (TCG→ACG) ‡	PP_1551 →	replication protein O

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,756,071	0	T	A	5.2%	979.5 / 28.2	310	S147T (TCG→ACG) ‡	PP_1551	replication protein O
Reads supporting (aligned to +/- strand): ref base T (150/140); new base A (8/8); total (162/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-01

Only 100 of 336 total aligned reads displayed.

ACCCGAAGGACTGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGCAAG  >  NC_002947/1755922‑1756220

 cccGAAGGACTGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATcttccttc                                                                                                                                                      <  1:976617/150‑1 (MQ=255)

  ccGAAGGACTGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCg                                                                                                                                                     >  1:5665401/1‑150 (MQ=255)

  ccGAAGGACTGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCg                                                                                                                                                     <  2:4473015/150‑1 (MQ=255)

       ggACTGTATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGa                                                                                                                                                <  1:3432659/150‑1 (MQ=255)

           tGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGtt                                                                                                                                            >  2:5483862/1‑150 (MQ=255)

           tGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTGTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGtt                                                                                                                                            >  2:2384488/1‑150 (MQ=255)

            ggATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTa                                                                                                                                           <  1:1092221/150‑1 (MQ=255)

            ggATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCGAAGGAAGAtttac                                                                                                                                           <  2:728982/150‑5 (MQ=255)

              aTTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCGAAGGAAGAT‑TTACAt                                                                                                                                        <  1:2813068/150‑1 (MQ=255)

                ttACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTCCATg                                                                                                                                       >  2:6544163/1‑150 (MQ=255)

                   cGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAAGCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAACATCTTCCGAAGGAAGAT‑TTACAtaggg                                                                                                                                    <  1:6193813/150‑5 (MQ=255)

                    gTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGACTCGGCTCAGAGTCGAAACAGACCAAACCCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAACATCTTCCGAAGGAAGAT‑TTACAtagggg                                                                                                                                   <  2:1499910/150‑6 (MQ=255)

                    gTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGcccc                                                                                                                                   <  2:679113/150‑1 (MQ=255)

                    gTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGcccc                                                                                                                                   >  1:1817027/1‑150 (MQ=255)

                    gTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCATCGGAAGATGGTACATGcccc                                                                                                                                   >  2:438221/1‑150 (MQ=255)

                     ttGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGccccc                                                                                                                                  <  1:1670731/150‑1 (MQ=255)

                     ttGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCCAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGtt                                                                                                                                            <  1:6764664/140‑1 (MQ=255)

                        aTCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCa                                                                                                                               <  2:4197888/150‑1 (MQ=255)

                        aTCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCa                                                                                                                               <  1:3613221/150‑1 (MQ=255)

                          ccGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCCGc                                                                                                                             >  1:6798850/1‑150 (MQ=255)

                            gAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCga                                                                                                                           <  1:68923/150‑1 (MQ=255)

                              aaCAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCgagc                                                                                                                         <  1:1246683/150‑1 (MQ=255)

                                   aCCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCgagccggag                                                                                                                    >  2:1598720/1‑150 (MQ=255)

                                   aCCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCgagccggag                                                                                                                    >  1:599360/1‑150 (MQ=255)

                                     cAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCgagccggagcc                                                                                                                  >  2:3981164/1‑150 (MQ=255)

                                         aCAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCCGCgagccggacccccca                                                                                                              >  1:100465/1‑146 (MQ=255)

                                           aGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGGCTCCCTTCTTTATTCTAAGAAAGAAACCCCCGATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTcc                                                                                                            >  1:2835070/1‑150 (MQ=255)

                                             ccGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTATGAAAGAAACCCCCGATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCCGCGAGCCGGCGCCGCCTCCgg                                                                                                          >  1:1490905/1‑150 (MQ=255)

                                              cGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGc                                                                                                         <  2:3197981/150‑1 (MQ=255)

                                               gACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATcttcctt                                                                                                                                                       >  2:2825326/1‑103 (MQ=255)

                                               gACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGcc                                                                                                        <  2:1327067/150‑1 (MQ=255)

                                               gACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGcc                                                                                                        >  1:1786718/1‑150 (MQ=255)

                                                 cTCGGCTGAAGGGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTACTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCaa                                                                                                      <  2:6420319/150‑1 (MQ=255)

                                                    ggCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTAAATGCCCCCCCCCCGAGCCCGGGCCGCCGCCGGCCAAGGa                                                                                                   >  1:3251926/1‑150 (MQ=255)

                                                     gcagAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAACCCCGCCGACTCCCTTCTGTATTCTAAGAAAGAAACCCCCTATGGAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCCGCGAGCCGGAGCCGACTCCGGCCAAGGAt                                                                                                  <  2:4943327/147‑1 (MQ=255)

                                                     gCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGGGCCGGAGTCGGCTCCGGctcgctgg                                                                                                  <  1:1389688/150‑8 (MQ=255)

                                                     gCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGAt                                                                                                  <  1:545421/150‑1 (MQ=255)

                                                       tGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGa                                                                                                <  1:3144461/150‑1 (MQ=255)

                                                        gAAGTGTTCCGAATCGTCTCTGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTCCATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAg                                                                                               <  1:799570/150‑1 (MQ=255)

                                                            tGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGTGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCa                                                                                           >  1:2613238/1‑150 (MQ=255)

                                                              gTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAg                                                                                         >  2:765117/1‑150 (MQ=255)

                                                               tCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAgg                                                                                        <  2:3832720/150‑1 (MQ=255)

                                                                  gAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCCGCGCCGACGCCGGCCAACGATGAGCGCAAGGcgc                                                                                     >  1:3529991/1‑148 (MQ=255)

                                                                   aaTCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTcc                                                                                    >  2:2168361/1‑150 (MQ=255)

                                                                   aaTCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTcc                                                                                    <  2:3291657/150‑1 (MQ=255)

                                                                    aTCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTccc                                                                                   <  2:2517139/150‑1 (MQ=255)

                                                                        gCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCg                                                                               <  1:5141012/150‑1 (MQ=255)

                                                                        gCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTATTTATTCTAAGAAAGAAACCCCCTATGTCA‑ATCTTCCTGCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCCACGCCGGCCAACGATCAGCGCAAGGCTCCCATCg                                                                               >  2:5218820/1‑150 (MQ=255)

                                                                         cTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCgg                                                                              <  2:6269929/150‑1 (MQ=255)

                                                                          tCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGc                                                                             >  2:2439823/1‑150 (MQ=255)

                                                                               gTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAgc                                                                        <  2:683078/150‑1 (MQ=255)

                                                                                  gAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACGCCGGCCAAGGATGAGCGCAAGGCTCCCATCGGCCAAgccgc                                                                     >  1:4644418/1‑150 (MQ=255)

                                                                                         aCCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTg                                                              <  1:6555265/150‑1 (MQ=255)

                                                                                         aCCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCAAGCTg                                                              >  1:6258664/1‑150 (MQ=255)

                                                                                          ccAAAACCGCCGACTCCCTTCTTTATTCTCAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGCTGAGCGCAAGGCTCCCTGCGGCAAAGCCGCCAGGCTgg                                                             >  2:5154817/1‑150 (MQ=255)

                                                                                          ccAAAACCGCCGACTCCCTTCTTTATTCTAAGATAGACACCCCCTATGTAA‑ATCTTCCGGCGGAAGATGTGGCATGCCGCCCCAGCGGGCCGGGGCCGACTCCGGCCCAGGATGAGCGCGAGGCTCCCTGCGGCAAAGCCGCCCTGCTgg                                                             >  2:3960044/1‑150 (MQ=255)

                                                                                            aaaaCCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGcc                                                           <  2:599360/150‑1 (MQ=255)

                                                                                              aaCCGCCGACTCCCTTCTTTATTCTAAGAAAGACACCCCCTATGTAA‑AGCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACCCCGCCCCACGATGAGCGCCCCGCGCCCTTCCCCAAATCCGCCCCGCGGGCCgc                                                         >  1:2966235/1‑149 (MQ=255)

                                                                                                       cTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCa                                                <  1:2756359/150‑1 (MQ=255)

                                                                                                        tCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCAc                                               >  2:5769555/1‑150 (MQ=255)

                                                                                                        tCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCATCGGCAAAGCCCCCAAGCTGGCCGACAAAACGCAc                                               >  2:3030837/1‑150 (MQ=255)

                                                                                                        tCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCCGAGCCGACGCCCGCCCACGATGAGCGCAAGGCTCCCGTCGGCACAGCCGCCAAGCTGGCCCACAAACCCCAc                                               >  1:2639122/1‑150 (MQ=255)

                                                                                                          ccTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAAACCGCACgg                                             >  2:5377967/1‑150 (MQ=255)

                                                                                                          ccTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCAAGCTGGCCGACAATCCGCACgg                                             >  1:1552153/1‑150 (MQ=255)

                                                                                                            ttcttTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCAAGCTGGCCGACACACCGCACGGGc                                           >  1:4341908/1‑150 (MQ=255)

                                                                                                            ttcttTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCCGAGCCGACTCCCGCCAAGGATGAGCGCAAGGCTCCCGTCGGCAAACCCGCCATGCTGGCCGACAATCCGCACGGGc                                           >  1:2239259/1‑150 (MQ=255)

                                                                                                            ttcttTATTCTAAGAAAGAAACCCCCTATGTAA‑ATATTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCCACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGc                                           >  2:3853807/1‑150 (MQ=255)

                                                                                                                   ttCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGa                                    <  1:851210/150‑1 (MQ=255)

                                                                                                                      tAAGAAAGATACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTAAATGCCACCCCAGCGAGCCGGAGCCGACACCGGCCAAGGATGAGCGCAAGGCACCCATCGGCAAAGCCGCCAAGCACGCCGACAAACCGCACGGGCTCGATGaaac                                 >  2:2667083/1‑148 (MQ=255)

                                                                                                                      tAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGCCTCCGGCCAAGGATGAGCGCAACGCACCCAACGGCAAAGCCGCCCAGCTGGCCGACAATCCCCACCGGCGCCATGAATc                                 >  1:3773804/1‑150 (MQ=255)

                                                                                                                      tAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACACCGGCCAACGATGAGCGCAAGGCTCCCATCGGCAAAGCCGCCCTGCTGGCCCACAAACCGCACGCGCACGATGAATc                                 >  1:4572124/1‑150 (MQ=255)

                                                                                                                      tAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCCGACCCGACTCCCGCCAAGGCTGAGCGCAAGGCACCCTTCGGCAAAGCCGCCCTGCTGGCCGACAATCCCCACGGGCTCGATGaaac                                 >  2:3721791/1‑148 (MQ=255)

                                                                                                                      tAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACAAGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGcc                                                           >  2:2776249/1‑124 (MQ=255)

                                                                                                                       aagaaagaAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATcc                                >  2:5637806/1‑150 (MQ=255)

                                                                                                                        agaaagaaACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATccc                               <  2:1950776/150‑1 (MQ=255)

                                                                                                                         gaaagaaaCCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCt                              <  1:5348131/150‑1 (MQ=255)

                                                                                                                           aagaaaCCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGa                            <  1:1946373/150‑1 (MQ=255)

                                                                                                                            agaaaCCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGACCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGAATACCGCAAGGCTCCCATCGGCAAAGCCGCCATGCTGGCCGACAAACCGCACGGGCACGATGAATACCGGAt                           >  2:1123399/1‑150 (MQ=255)

                                                                                                                             gaaaCCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATc                          <  2:3548480/150‑1 (MQ=255)

                                                                                                                                aCCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTACGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATACCTGATCGCt                       >  2:5937286/1‑150 (MQ=255)

                                                                                                                                aCCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACGCCGGCCAAGGATGAGCGCAAGGCTCCCGTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCt                       >  2:850491/1‑150 (MQ=255)

                                                                                                                                  ccccTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGa                     <  2:4907942/150‑1 (MQ=255)

                                                                                                                                  ccccTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGa                     <  1:3981164/150‑1 (MQ=255)

                                                                                                                                       aTGTAACATCTTCCGAAGGAAGAT‑TTACATGCCCCCCCAGCGAGCCGGAGCCGACTACGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATACCTGATCTCTGATTaat                >  1:1202595/1‑148 (MQ=255)

                                                                                                                                        tGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACtt               >  1:5919517/1‑150 (MQ=255)

                                                                                                                                        tGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACtt               <  1:2168361/150‑1 (MQ=255)

                                                                                                                                          taacaTCTTCCGAAGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTAATTg              >  1:3827211/5‑150 (MQ=255)

                                                                                                                                          taacaTCTTCCGAAGGAAGAT‑TTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTgg             >  1:4191561/5‑150 (MQ=255)

                                                                                                                                          tAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTgg             <  1:3109799/150‑1 (MQ=255)

                                                                                                                                          tAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTgg             >  2:3376565/1‑150 (MQ=255)

                                                                                                                                          tAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTgg             <  1:5871403/150‑1 (MQ=255)

                                                                                                                                          tAA‑ATCTTCCGTCGGAAGATGTTACATGCCCCCCCCGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTgg             <  2:6136725/150‑1 (MQ=255)

                                                                                                                                             caTCTTCCGAAGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCt           >  1:6065197/2‑150 (MQ=255)

                                                                                                                                              aTCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTg          >  2:1735338/1‑150 (MQ=255)

                                                                                                                                                 ttccttcGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTcc       >  1:4032813/1‑150 (MQ=255)

                                                                                                                                                 ttccttcGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTcc       <  2:45536/150‑1 (MQ=255)

                                                                                                                                                   ccttcGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGc     >  2:2607579/1‑150 (MQ=255)

                                                                                                                                                    cttcGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGCa    >  1:491381/1‑150 (MQ=255)

                                                                                                                                                     ttcGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGCaa   >  2:4087377/1‑150 (MQ=255)

                                                                                                                                                     ttcGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGCaa   >  1:1849038/1‑150 (MQ=255)

ACCCGAAGGACTGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGCAAG  >  NC_002947/1755922‑1756220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`