BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,850,778	T→G	5.0%	S26R (AGT→AGG)	pfeS‑II →	histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,850,778	0	T	G	5.0%	1056.8 / 26.6	307	S26R (AGT→AGG)	pfeS‑II	histidine kinase
Reads supporting (aligned to +/- strand): ref base T (151/140); new base G (3/13); total (154/153)
Fisher's exact test for biased strand distribution p-value = 1.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

ATCACGAACGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGCATCA  >  NC_002947/1850630‑1850927

   aCGAACGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGACGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTtata                                                                                                                                                   >  1:5528153/1‑150 (MQ=255)

      aaCGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCt                                                                                                                                                <  1:2460937/150‑1 (MQ=255)

        cGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTgg                                                                                                                                              <  1:6610544/150‑1 (MQ=255)

        cGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTgg                                                                                                                                              <  2:2701214/150‑1 (MQ=255)

         ggCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTggg                                                                                                                                             >  2:1916560/1‑150 (MQ=255)

          gCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGGTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTgggg                                                                                                                                            >  2:4557889/1‑150 (MQ=255)

            tgtcgagCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACATGGGGcc                                                                                                                                          >  2:2896152/7‑150 (MQ=255)

            aGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGcc                                                                                                                                          >  1:705190/1‑150 (MQ=255)

            aGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGcc                                                                                                                                          >  1:5085069/1‑150 (MQ=255)

             gATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTGAGGCCg                                                                                                                                         <  2:527057/150‑1 (MQ=255)

              aTCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCgg                                                                                                                                        >  1:5990379/1‑150 (MQ=255)

              aTCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTGAGGCCga                                                                                                                                        <  1:1462064/150‑2 (MQ=255)

              aTCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTGAGGCCga                                                                                                                                        <  2:1508489/150‑2 (MQ=255)

              aTCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGACCGGCCCCAGGTATAACTGAGGCCga                                                                                                                                        <  2:2435973/150‑2 (MQ=255)

                   cACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACAt                                                                                                                                   <  2:1235867/150‑1 (MQ=255)

                    aCCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATg                                                                                                                                  <  2:4225307/150‑1 (MQ=255)

                    aCCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATg                                                                                                                                  <  1:1180723/150‑1 (MQ=255)

                    aCCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATg                                                                                                                                  <  1:1551457/150‑1 (MQ=255)

                              gCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGa                                                                                                                        >  1:5287139/1‑150 (MQ=255)

                              gCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGa                                                                                                                        >  2:2660550/1‑150 (MQ=255)

                               cAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGaa                                                                                                                       <  2:3097309/150‑1 (MQ=255)

                                  gggTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGc                                                                                                                    >  2:4497280/1‑150 (MQ=255)

                                  gggTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGc                                                                                                                    >  2:1069281/1‑150 (MQ=255)

                                      tACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTc                                                                                                                <  2:6178899/150‑1 (MQ=255)

                                      tACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTc                                                                                                                <  2:2784832/150‑1 (MQ=255)

                                      tACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTc                                                                                                                <  2:6354736/150‑1 (MQ=255)

                                           gCTCAGTGCCAGCGAGGCGGAGTGAACCATTCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTTCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCtctc                                                                                                           <  1:5714336/150‑1 (MQ=255)

                                           gCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCtctc                                                                                                           >  2:2267479/1‑150 (MQ=255)

                                           gCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCtctc                                                                                                           >  1:4722164/1‑150 (MQ=255)

                                           gCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCtctc                                                                                                           <  1:6431422/150‑1 (MQ=255)

                                            cTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCg                                                                                                          <  1:2278244/150‑1 (MQ=255)

                                            cTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCg                                                                                                          <  1:452761/150‑1 (MQ=255)

                                             tCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCgg                                                                                                         >  1:1263121/1‑150 (MQ=255)

                                             tCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCAGCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCgg                                                                                                         >  2:3599695/1‑150 (MQ=255)

                                              cAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGa                                                                                                        <  2:5044025/150‑1 (MQ=255)

                                                      gCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCAc                                                                                                <  2:56408/150‑1 (MQ=255)

                                                      gCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCAc                                                                                                <  2:827177/150‑1 (MQ=255)

                                                        gAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGc                                                                                              >  2:2544161/1‑150 (MQ=255)

                                                         aGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGcc                                                                                             >  2:4091134/1‑150 (MQ=255)

                                                          ggCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCa                                                                                            <  1:4168891/150‑1 (MQ=255)

                                                           gCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAg                                                                                           <  1:5357497/150‑1 (MQ=255)

                                                           gCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAg                                                                                           <  2:3208942/150‑1 (MQ=255)

                                                             ggAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGAc                                                                                         >  2:3987133/1‑150 (MQ=255)

                                                             ggAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGAc                                                                                         >  1:2372920/1‑150 (MQ=255)

                                                              gAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGAcc                                                                                        >  1:3957788/1‑150 (MQ=255)

                                                                     ccATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCggg                                                                                 <  1:4895676/150‑1 (MQ=255)

                                                                             gACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATgcc                                                                         >  1:1042438/1‑150 (MQ=255)

                                                                             gACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATgcc                                                                         <  1:2658450/150‑1 (MQ=255)

                                                                                 gCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGccgccg                                                                     >  2:6866643/1‑150 (MQ=255)

                                                                                  ccATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGa                                                                    >  1:6181207/1‑150 (MQ=255)

                                                                                  ccATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGa                                                                    >  2:489256/1‑150 (MQ=255)

                                                                                     ttCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGc                                                                 >  2:1352327/1‑150 (MQ=255)

                                                                                        gCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGa                                                              <  2:2930748/150‑1 (MQ=255)

                                                                                         cTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGaa                                                             <  1:2754537/150‑1 (MQ=255)

                                                                                          tGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAAc                                                            >  1:5923584/1‑150 (MQ=255)

                                                                                          tGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAAc                                                            >  1:4327574/1‑150 (MQ=255)

                                                                                            ttCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAg                                                          >  1:1956701/1‑150 (MQ=255)

                                                                                              cTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGc                                                        >  2:2589580/1‑150 (MQ=255)

                                                                                                     cTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCg                                                                                                                                         >  1:362205/1‑62 (MQ=255)

                                                                                                      tGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCa                                                >  2:2123884/1‑150 (MQ=255)

                                                                                                        gCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGt                                              <  1:6299954/150‑1 (MQ=255)

                                                                                                        gCCATCTTGCTGGTGGGATTCTGCCTGCTGATGATCGGCCTCTGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCAGGCGCAGt                                              >  2:3973183/1‑150 (MQ=255)

                                                                                                           aTCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTggc                                           <  2:3381011/150‑1 (MQ=255)

                                                                                                           aTCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTggc                                           <  1:4063070/150‑1 (MQ=255)

                                                                                                           aTCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTggc                                           <  2:761760/150‑1 (MQ=255)

                                                                                                              ttGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCa                                                >  2:3747280/1‑142 (MQ=255)

                                                                                                              ttGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTggcggg                                        >  2:5626216/1‑150 (MQ=255)

                                                                                                              ttGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTggcggg                                        >  1:29663/1‑150 (MQ=255)

                                                                                                               tGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGACCAGGCCTGGCGCAGTggcgggc                                       >  1:3504564/1‑150 (MQ=255)

                                                                                                                gCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTggcgggcg                                      >  1:1707750/1‑150 (MQ=255)

                                                                                                                  tggtggGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGcgggcggg                                    >  1:3365758/1‑150 (MQ=255)

                                                                                                                    gtggGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCa                                  >  1:5848830/1‑150 (MQ=255)

                                                                                                                    gtggGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCa                                  <  1:1327398/150‑1 (MQ=255)

                                                                                                                          ttCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTg                            >  2:3872522/1‑150 (MQ=255)

                                                                                                                           tCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTgg                           <  1:6512404/150‑1 (MQ=255)

                                                                                                                             tgcctgcTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGAc                         >  2:1667970/1‑150 (MQ=255)

                                                                                                                               cctgcTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCa                       >  1:2740659/1‑150 (MQ=255)

                                                                                                                               cctgcTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCa                       <  2:4708135/150‑1 (MQ=255)

                                                                                                                                 tgctgATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgt                     >  2:2148335/1‑150 (MQ=255)

                                                                                                                                 tgctgATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgt                     >  2:2112040/1‑150 (MQ=255)

                                                                                                                                 tgctgATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgt                     <  2:1272786/150‑1 (MQ=255)

                                                                                                                                  gctgATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTg                                                                                     <  1:1885911/85‑1 (MQ=255)

                                                                                                                                    tgatgaTCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATTCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgtggg                  >  1:1079339/1‑150 (MQ=255)

                                                                                                                                     gatgatCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgtgggt                 >  1:1407241/1‑150 (MQ=255)

                                                                                                                                     gatgatCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgtgggt                 >  1:307314/1‑150 (MQ=255)

                                                                                                                                       gtgccGGCCCCAGGTATAACTGAGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgtgggtgg               >  1:914628/5‑150 (MQ=255)

                                                                                                                                        gatCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTggc              >  1:1623554/1‑150 (MQ=255)

                                                                                                                                         atCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTggcg             <  1:6769762/150‑1 (MQ=255)

                                                                                                                                          tCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTggcgg            >  2:3498977/1‑150 (MQ=255)

                                                                                                                                          tCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTggcgg            >  2:2306430/1‑150 (MQ=255)

                                                                                                                                              ccTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCAt        <  1:3754291/150‑1 (MQ=255)

                                                                                                                                              ccTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCAt        <  1:333961/150‑1 (MQ=255)

                                                                                                                                              ccTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCAt        <  2:4120782/150‑1 (MQ=255)

                                                                                                                                               cTCAGTTATACCTGGGGCCGGCGCATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATg       >  2:6597684/1‑150 (MQ=255)

                                                                                                                                                tCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGc      >  1:5211252/1‑150 (MQ=255)

                                                                                                                                                tCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGc      >  2:5481942/1‑150 (MQ=255)

                                                                                                                                                tCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGc      >  1:4596167/1‑150 (MQ=255)

                                                                                                                                                tCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGc      >  2:1873869/1‑150 (MQ=255)

                                                                                                                                                tCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGc      >  1:2910172/1‑150 (MQ=255)

                                                                                                                                                    ttATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGCATCa  <  2:5931518/150‑1 (MQ=255)

ATCACGAACGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGCATCA  >  NC_002947/1850630‑1850927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`