BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,850,787	G→A	5.2%	W29* (TGG→TGA)	pfeS‑II →	histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,850,787	0	G	A	5.2%	983.9 / 25.1	305	W29* (TGG→TGA)	pfeS‑II	histidine kinase
Reads supporting (aligned to +/- strand): ref base G (155/134); new base A (3/13); total (158/147)
Fisher's exact test for biased strand distribution p-value = 8.63e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

CGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGCATCAGCGCGAACG  >  NC_002947/1850638‑1850936

cGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTgg                                                                                                                                                       <  1:6610552/150‑1 (MQ=255)

cGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTgg                                                                                                                                                       <  2:2701214/150‑1 (MQ=255)

cGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTgg                                                                                                                                                       <  2:6820069/150‑1 (MQ=255)

   cAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTGAGGc                                                                                                                                                    <  2:4545684/150‑1 (MQ=255)

   cAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTGAGGc                                                                                                                                                    <  2:3572335/150‑1 (MQ=255)

    tgtcgagCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGcc                                                                                                                                                   >  2:2896207/7‑150 (MQ=255)

    aGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGcc                                                                                                                                                   >  2:2308973/1‑150 (MQ=255)

    aGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGcc                                                                                                                                                   >  2:719113/1‑150 (MQ=255)

     gATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCg                                                                                                                                                  <  1:5147738/150‑1 (MQ=255)

     gATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTGAGGCCg                                                                                                                                                  <  2:527057/150‑1 (MQ=255)

     gATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTGAGGCCg                                                                                                                                                  <  2:3169873/150‑1 (MQ=255)

      aTCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTGAGGCCga                                                                                                                                                 <  1:1462065/150‑2 (MQ=255)

      aTCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGACCGGCCCCAGGTATAACTGAGGCCga                                                                                                                                                 <  2:2436074/150‑2 (MQ=255)

           cACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACAt                                                                                                                                            <  1:5799859/150‑1 (MQ=255)

            aCCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATg                                                                                                                                           <  1:1180723/150‑1 (MQ=255)

             ccGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATgg                                                                                                                                          <  2:2133527/150‑1 (MQ=255)

                      gCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGa                                                                                                                                 >  1:5287139/1‑150 (MQ=255)

                      gCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGa                                                                                                                                 >  1:4119479/1‑150 (MQ=255)

                      gCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGa                                                                                                                                 >  1:3299/1‑150 (MQ=255)

                      gCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGa                                                                                                                                 >  2:2660550/1‑150 (MQ=255)

                       cAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGaa                                                                                                                                <  2:2933835/150‑1 (MQ=255)

                       cAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGaa                                                                                                                                <  1:5672788/150‑1 (MQ=255)

                       cAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGaa                                                                                                                                <  2:1013054/150‑1 (MQ=255)

                        aaGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAAt                                                                                                                               <  2:2821503/150‑1 (MQ=255)

                          gggTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGc                                                                                                                             <  1:734636/150‑1 (MQ=255)

                              tACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTc                                                                                                                         <  2:1323843/150‑1 (MQ=255)

                              tACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTc                                                                                                                         <  2:6450287/150‑1 (MQ=255)

                                     tCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCgg                                                                                                                  >  1:1263121/1‑150 (MQ=255)

                                      cAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGa                                                                                                                 <  2:5076169/150‑1 (MQ=255)

                                            cAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGc                                                                                                           >  2:4341728/1‑150 (MQ=255)

                                             aGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCa                                                                                                          <  1:1570701/150‑1 (MQ=255)

                                              gCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCAc                                                                                                         <  2:56408/150‑1 (MQ=255)

                                                gAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGc                                                                                                       >  2:2544161/1‑150 (MQ=255)

                                                 aGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGcc                                                                                                      >  2:4091134/1‑150 (MQ=255)

                                                   gCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAg                                                                                                    <  1:5357497/150‑1 (MQ=255)

                                                      gAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGAcc                                                                                                 >  1:3957825/1‑150 (MQ=255)

                                                        gTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCtt                                                                                               >  2:2006102/1‑150 (MQ=255)

                                                           aaCCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCg                                                                                            >  1:2759264/1‑150 (MQ=255)

                                                            aCCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCgg                                                                                           <  1:657667/150‑1 (MQ=255)

                                                              cATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCgggg                                                                                         >  1:6782661/1‑150 (MQ=255)

                                                              cATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCgggg                                                                                         <  2:3423439/150‑1 (MQ=255)

                                                              cATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCgggg                                                                                         >  1:4275745/1‑150 (MQ=255)

                                                              cATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCgggg                                                                                         >  2:6222529/1‑150 (MQ=255)

                                                                 gCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGTCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTa                                                                                      >  2:3923956/1‑150 (MQ=255)

                                                                  cTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTAt                                                                                     >  1:4379108/1‑150 (MQ=255)

                                                                  cTCGACCGCCATTCGCTGGTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTACCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTAt                                                                                     >  2:1560288/1‑150 (MQ=255)

                                                                   tCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATg                                                                                    >  1:6032469/1‑150 (MQ=255)

                                                                     gACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATgcc                                                                                  >  1:1042438/1‑150 (MQ=255)

                                                                     gACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATgcc                                                                                  <  1:2658450/150‑1 (MQ=255)

                                                                        cGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAAc                                                                                                                                      >  2:1885899/1‑95 (MQ=255)

                                                                          ccATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGa                                                                             >  1:6181338/1‑150 (MQ=255)

                                                                                 cTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGaa                                                                      <  1:2754515/150‑1 (MQ=255)

                                                                                 cTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGaa                                                                      <  2:1429530/150‑1 (MQ=255)

                                                                                 cTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGaa                                                                      <  1:477148/150‑1 (MQ=255)

                                                                                  tGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAAc                                                                     >  1:5923584/1‑150 (MQ=255)

                                                                                    ttCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAg                                                                   >  1:1956701/1‑150 (MQ=255)

                                                                                      cTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGc                                                                 >  2:2589580/1‑150 (MQ=255)

                                                                                      cTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGc                                                                 <  2:1242236/150‑1 (MQ=255)

                                                                                             cTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCg                                                                                                                                                  >  1:362205/1‑62 (MQ=255)

                                                                                               ggCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAg                                                        >  1:2217326/1‑150 (MQ=255)

                                                                                                 ccATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTg                                                      <  1:3036723/150‑1 (MQ=255)

                                                                                                  cATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTgg                                                     >  1:5006721/1‑150 (MQ=255)

                                                                                                   aTCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCt                                                                         <  1:1638009/129‑1 (MQ=255)

                                                                                                   aTCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTggc                                                    <  2:3380902/150‑1 (MQ=255)

                                                                                                   aTCTTGCTGGAGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTggc                                                    >  2:4816881/1‑150 (MQ=255)

                                                                                                      ttGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCa                                                         >  2:3747280/1‑142 (MQ=255)

                                                                                                      ttGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTggcggg                                                 >  2:5626216/1‑150 (MQ=255)

                                                                                                      ttGCTGGTGGGCTTCTACCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTggcggg                                                 <  2:5007915/150‑1 (MQ=255)

                                                                                                       tGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGACCAGGCCTGGCGCAGTggcgggc                                                >  1:3504564/1‑150 (MQ=255)

                                                                                                        gCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTggcgggcg                                               >  1:2428461/1‑150 (MQ=255)

                                                                                                        gCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTggcgggcg                                               >  1:1707750/1‑150 (MQ=255)

                                                                                                            gtggGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCa                                           <  1:1327398/150‑1 (MQ=255)

                                                                                                               ggCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAggg                                        <  2:398666/150‑1 (MQ=255)

                                                                                                                  ttcttcCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTg                                     >  2:180897/1‑150 (MQ=255)

                                                                                                                  ttCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTg                                     >  1:3349971/1‑150 (MQ=255)

                                                                                                                  ttCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTg                                     <  2:4668831/150‑1 (MQ=255)

                                                                                                                      gcctgcTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGAcc                                 >  2:1202523/1‑150 (MQ=255)

                                                                                                                        ctgctgATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAg                               <  2:2183021/150‑1 (MQ=255)

                                                                                                                         tgctgATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgt                              >  1:4636450/1‑150 (MQ=255)

                                                                                                                         tgctgATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgt                              >  2:2112139/1‑150 (MQ=255)

                                                                                                                          gctgATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTg                                                                                              <  1:1885911/85‑1 (MQ=255)

                                                                                                                            tgatgaTCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATTCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgtggg                           >  1:1079339/1‑150 (MQ=255)

                                                                                                                             gatgatCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgtgggt                          >  1:1407241/1‑150 (MQ=255)

                                                                                                                             atgtgccGGCCCCAGGTATAACTGAGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgtgggt                          >  2:2738662/7‑150 (MQ=255)

                                                                                                                               tgatCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgtgggtgg                        <  2:3790885/150‑1 (MQ=255)

                                                                                                                                     gCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCa                  >  1:228858/1‑150 (MQ=255)

                                                                                                                                      ccTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCAt                 <  2:4120782/150‑1 (MQ=255)

                                                                                                                                      ccTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCAt                 <  1:3754291/150‑1 (MQ=255)

                                                                                                                                       cTCAGTTATACCTTGGGCCGTCTATTGGAAACAGAGAATGCCTTCCTCTCGGTGCCTGCACGCCAGACCTTGCGGGGTTATGCCGCCGTAGCTGAACTGGGCTTGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGTTGTCGTCCATg                <  2:781853/150‑1 (MQ=255)

                                                                                                                                        tCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGc               >  1:5211257/1‑150 (MQ=255)

                                                                                                                                        tCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGc               <  1:4570840/150‑1 (MQ=255)

                                                                                                                                        tCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGc               >  2:5481942/1‑150 (MQ=255)

                                                                                                                                        tCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGc               >  1:2957769/1‑150 (MQ=255)

                                                                                                                                        tCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGc               >  1:6485168/1‑150 (MQ=255)

                                                                                                                                          aGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGCAt             <  1:6312014/150‑1 (MQ=255)

                                                                                                                                            ttATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGCATCa           <  2:5931518/150‑1 (MQ=255)

                                                                                                                                              ataCCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGCATCAgc         <  1:2672695/150‑1 (MQ=255)

                                                                                                                                                    ggggCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGCATCAGCGCGAAc   >  1:3899001/1‑150 (MQ=255)

                                                                                                                                                    ggggCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGCATCAGCGCGAAc   >  2:4025338/1‑150 (MQ=255)

                                                                                                                                                     gggCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGCATCAGCGCGAACg  <  2:2288071/150‑1 (MQ=255)

CGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGCCGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGCATCAGCGCGAACG  >  NC_002947/1850638‑1850936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`