Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,168,427 T→A 6.0% I99N (ATT→AAT)  PP_5482 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,168,4270TA 6.0% 935.5 / 31.2 283I99N (ATT→AAT) PP_5482hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (157/108);  new base A (11/6);  total (169/114)
Fisher's exact test for biased strand distribution p-value = 8.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 313 total aligned reads displayed.

GGGGTAGTGGGGCAACTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAATTTTGGCCT  >  NC_002947/2168279‑2168575
                                                                                                                                                    |                                                                                                                                                    
ggggTAGTGGGGCAACTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCAtt                                                                                                                                                     <  2:6705917/150‑1 (MQ=255)
   gTAGTGGGGCAACTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGAt                                                                                                                                                  <  1:3753901/150‑1 (MQ=255)
   gTAGTGGGGCAACTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGAt                                                                                                                                                  <  1:2830451/150‑1 (MQ=255)
   gTAGTGGGGCAACTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGAt                                                                                                                                                  <  1:767504/150‑1 (MQ=255)
   gTAGTGGGGCAACTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCAATGAt                                                                                                                                                  <  1:4084168/150‑1 (MQ=255)
     aGTGGGGCAACTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGc                                                                                                                                                <  2:5419453/150‑1 (MQ=255)
       tGGGGCAACTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCt                                                                                                                                              >  1:4589279/1‑150 (MQ=255)
         gggCAACTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCttt                                                                                                                                            >  2:797727/1‑150 (MQ=255)
         gggCAACTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCttt                                                                                                                                            >  2:2469690/1‑150 (MQ=255)
         gggCAACTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCttt                                                                                                                                            >  2:1718304/1‑150 (MQ=255)
          ggCAACTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTc                                                                                                                                           >  1:6718115/1‑150 (MQ=255)
             aaCTGGTGCCTGCACTCGAATTCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGc                                                                                                                                        <  2:3814419/150‑1 (MQ=255)
             aaCTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGc                                                                                                                                        <  2:3040317/150‑1 (MQ=255)
               cTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCta                                                                                                                                      <  1:3929848/150‑1 (MQ=255)
                tGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGGGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCTGCtat                                                                                                                                     >  2:6619564/1‑150 (MQ=255)
                tGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCtat                                                                                                                                     >  2:5236051/1‑150 (MQ=255)
                tGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCtat                                                                                                                                     >  2:5082838/1‑150 (MQ=255)
                tGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCtat                                                                                                                                     <  2:845692/150‑1 (MQ=255)
                  gTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTatat                                                                                                                                   >  2:6384953/1‑150 (MQ=255)
                    gCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATAtgt                                                                                                                                 <  1:2953364/150‑1 (MQ=255)
                       tGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGa                                                                                                                              >  2:3861088/1‑150 (MQ=255)
                          aCTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCa                                                                                                                           >  1:5569892/1‑150 (MQ=255)
                           cTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCAt                                                                                                                          <  2:3189673/150‑1 (MQ=255)
                            tCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATg                                                                                                                         >  1:993192/1‑150 (MQ=255)
                            tCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATg                                                                                                                         >  1:4974573/1‑150 (MQ=255)
                            tCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCACAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATg                                                                                                                         >  2:5535934/1‑150 (MQ=255)
                             cGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATgg                                                                                                                        <  1:6856752/150‑1 (MQ=255)
                             cGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATgg                                                                                                                        <  1:3512843/150‑1 (MQ=255)
                              gAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGc                                                                                                                       >  2:444122/1‑150 (MQ=255)
                               aaTGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCg                                                                                                                      >  1:2620117/1‑150 (MQ=255)
                               aaTGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCg                                                                                                                      >  2:6792868/1‑150 (MQ=255)
                                 tGCTTTGAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGAc                                                                                                                    >  1:433451/1‑150 (MQ=255)
                                   cTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTc                                                                                                                  <  2:4631667/150‑1 (MQ=255)
                                   cTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTc                                                                                                                  <  2:2177898/150‑1 (MQ=255)
                                    tttAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCg                                                                                                                 >  2:3677029/1‑150 (MQ=255)
                                    tttAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCg                                                                                                                 >  2:2343713/1‑150 (MQ=255)
                                    tttAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCg                                                                                                                 >  2:6854370/1‑150 (MQ=255)
                                       aaaaCGGTAGTCATTGATCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCGGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCATCTATATGTGGACCATGGCGACTCGCAt                                                                                                              <  1:2083282/150‑1 (MQ=255)
                                         aaCGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTc                                                                                                            >  1:575855/1‑150 (MQ=255)
                                             gTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTg                                                                                                        >  2:4021936/1‑150 (MQ=255)
                                              tAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGc                                                                                                       <  2:3161545/150‑1 (MQ=255)
                                                gTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTg                                                                                                     <  2:4231514/150‑1 (MQ=255)
                                                gTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTg                                                                                                     <  1:3031858/150‑1 (MQ=255)
                                                              tAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGtt                                                                                       >  2:1962231/1‑150 (MQ=255)
                                                              tAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGtt                                                                                       <  2:188441/150‑1 (MQ=255)
                                                                          tgtTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGc                                                                           >  1:54736/1‑150 (MQ=255)
                                                                              aTCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCa                                                                       >  1:2338253/1‑150 (MQ=255)
                                                                               tCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCAc                                                                      >  1:4299297/1‑150 (MQ=255)
                                                                                   aGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTaa                                                                  >  1:2259436/1‑150 (MQ=255)
                                                                                    gCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTaaa                                                                 <  2:2903631/150‑1 (MQ=255)
                                                                                    gCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTaaa                                                                 <  1:2469690/150‑1 (MQ=255)
                                                                                       aTCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTc                                                              >  1:3657348/1‑150 (MQ=255)
                                                                                        tCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCg                                                             >  1:1012424/1‑150 (MQ=255)
                                                                                                  gCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCAtt                                                   <  1:1645012/150‑1 (MQ=255)
                                                                                                  gCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCAtt                                                   <  2:5847959/150‑1 (MQ=255)
                                                                                                   cTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCAttt                                                  >  1:6778800/1‑150 (MQ=255)
                                                                                                   cTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCAttt                                                  >  2:3471784/1‑150 (MQ=255)
                                                                                                    ttGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGTCGCACCTAAATTCGCAGCGGCATTTg                                                 >  2:6688033/1‑150 (MQ=255)
                                                                                                    ttGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTg                                                 >  1:3332324/1‑150 (MQ=255)
                                                                                                     tGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGa                                                <  1:4540671/150‑1 (MQ=255)
                                                                                                       aCGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGaaa                                              >  2:2982715/1‑150 (MQ=255)
                                                                                                       aCGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGaaa                                              <  1:3074094/150‑1 (MQ=255)
                                                                                                              tCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGt                                       <  1:1774774/150‑1 (MQ=255)
                                                                                                              tCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGt                                       >  1:5297798/1‑150 (MQ=255)
                                                                                                                gTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTg                                     >  1:6345342/1‑150 (MQ=255)
                                                                                                                 tAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGa                                    >  2:3574156/1‑150 (MQ=255)
                                                                                                                  aGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGaa                                   <  1:266005/150‑1 (MQ=255)
                                                                                                                    gCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAg                                 >  1:5517298/1‑150 (MQ=255)
                                                                                                                    gCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAg                                 >  1:6418729/1‑150 (MQ=255)
                                                                                                                     cAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGt                                >  1:4851002/1‑150 (MQ=255)
                                                                                                                      aGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGtt                               >  1:1163152/1‑150 (MQ=255)
                                                                                                                      aGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGtt                               >  2:336458/1‑150 (MQ=255)
                                                                                                                        gAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTc                             <  2:4175443/150‑1 (MQ=255)
                                                                                                                          aCTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTACACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCaa                           >  1:6092129/1‑150 (MQ=255)
                                                                                                                            tCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACg                         >  2:2495640/1‑150 (MQ=255)
                                                                                                                            tCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACg                         >  1:1499102/1‑150 (MQ=255)
                                                                                                                             cGAATTCGAGTATGAAGGAATCATTGATGACTTTCAGCTATATGTGGACCAAGGAGACTCGCATACGTTGCTGGAAACCAGTTCGTTGTGCACCGCAGCCGAACCTAAAATAGCAGCGGCAATTGAAACTACGGTTGAAAGATTCAACGc                        >  2:14788/1‑150 (MQ=255)
                                                                                                                              gAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCt                       >  2:4752845/1‑150 (MQ=255)
                                                                                                                                aTTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCAACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACTCTTa                     <  2:568818/150‑1 (MQ=255)
                                                                                                                                 ttCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTac                    >  1:534472/1‑150 (MQ=255)
                                                                                                                                 ttCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTac                    >  2:1552848/1‑150 (MQ=255)
                                                                                                                                   cGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTacac                  <  1:5688668/150‑1 (MQ=255)
                                                                                                                                    gAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACAcc                 >  2:6000002/1‑150 (MQ=255)
                                                                                                                                       tATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGt              <  1:4741523/150‑1 (MQ=255)
                                                                                                                                        tgaaaGGCATCAATGATGCCTTCCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTa             >  1:1346975/4‑150 (MQ=255)
                                                                                                                                        tgaaaGGCATCAATGATGCC‑TTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTaa            >  2:1244175/4‑150 (MQ=255)
                                                                                                                                          gAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTaaa           <  2:5301393/150‑1 (MQ=255)
                                                                                                                                          aaaGGCATCAATGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGGaaa           >  2:2677372/2‑150 (MQ=255)
                                                                                                                                              gCATCATTGATGCCTTTCAGCTATATGTGGACCAGGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAAtttt       <  2:4589279/150‑1 (MQ=255)
                                                                                                                                               catcatTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAATTTTg      <  2:1626477/150‑1 (MQ=255)
                                                                                                                                               catcatTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAATTTTg      <  2:5911889/150‑1 (MQ=255)
                                                                                                                                               catcatTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAATTTTg      <  2:632700/150‑1 (MQ=255)
                                                                                                                                               catcatTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAATTTTg      <  1:5082838/150‑1 (MQ=255)
                                                                                                                                               catcaaTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAATTTTg      >  2:4056891/1‑150 (MQ=255)
                                                                                                                                                atcatTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAATTTTgg     <  1:6619564/150‑1 (MQ=255)
                                                                                                                                                atcatTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAATTTTgg     <  1:6039324/150‑1 (MQ=255)
                                                                                                                                                atcaaTGATGCCTTCCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAATTTTgg     >  1:3438155/1‑150 (MQ=255)
                                                                                                                                                 tcatTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAATTTTGGc    <  2:490289/150‑1 (MQ=255)
                                                                                                                                                   atTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAATTTTGGCCt  <  2:3072872/150‑1 (MQ=255)
                                                                                                                                                   atTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCAACGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAATTTTGGCCt  >  2:6672798/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGGGTAGTGGGGCAACTGGTGCCTGCACTCGAATGCTTTAAAACGGTAGTCATTGAGCGAAATAACGATGAGCGTGTTATCGCAGCCATCAAAATTATGCTTGACGAGGCTCGTAGGCAGGAACTCGAATTCGAGTATGAAGGCATCATTGATGCCTTTCAGCTATATGTGGACCATGGCGACTCGCATTCGTTGCTGGATACCAGGTCGTTGTGCACCGCAGCCGCACCTAAATTCGCAGCGGCATTTGAAACTACGGTTGAAAGTTTCAACGCTTACACCAGTAAATTTTGGCCT  >  NC_002947/2168279‑2168575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑