Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,448,494 T→A 6.2% F6L (TTT→TTA) ‡ PP_2140 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,448,4940TA 6.2% 981.1 / 35.5 292F6L (TTT→TTA) ‡PP_2140hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (122/152);  new base A (5/13);  total (127/165)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 296 total aligned reads displayed.

GTGATCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACAAAG  >  NC_002947/2448345‑2448640
                                                                                                                                                     |                                                                                                                                                  
      cGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGcc                                                                                                                                              <  1:249116/150‑1 (MQ=255)
      cGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGcc                                                                                                                                              >  1:2245327/1‑150 (MQ=255)
       gCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGccc                                                                                                                                             <  2:3345838/150‑1 (MQ=255)
       gCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGccc                                                                                                                                             <  2:237201/150‑1 (MQ=255)
       gCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGccc                                                                                                                                             <  1:1756257/150‑1 (MQ=255)
        cTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGcccc                                                                                                                                            >  2:3701634/1‑150 (MQ=255)
        cTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGcccc                                                                                                                                            >  1:1045828/1‑150 (MQ=255)
          aCAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCaa                                                                                                                                          <  2:827239/150‑1 (MQ=255)
           cAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCaaa                                                                                                                                         <  2:2952528/150‑2 (MQ=255)
            aGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGCCCCaaat                                                                                                                                        <  1:5086147/150‑3 (MQ=255)
              ccGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAt                                                                                                                                      <  1:6220335/150‑1 (MQ=255)
               cGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAtt                                                                                                                                     <  1:2315184/150‑1 (MQ=255)
               cGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAtt                                                                                                                                     <  2:47771/150‑1 (MQ=255)
                gCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTCCGACGGCAATGCCTGGAAGGTTGAAGATACGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGCCCCaaatcaca                                                                                                                                    <  2:5066893/150‑7 (MQ=255)
                gCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGCCCCaaatcaca                                                                                                                                    <  2:2891885/150‑7 (MQ=255)
                 cAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTa                                                                                                                                   <  1:2673010/150‑1 (MQ=255)
                       cAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGc                                                                                                                             >  1:4138240/1‑150 (MQ=255)
                       cAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGc                                                                                                                             >  1:3821931/1‑150 (MQ=255)
                       cAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGc                                                                                                                             >  2:5361399/1‑150 (MQ=255)
                        aaGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCa                                                                                                                            <  2:3068681/150‑1 (MQ=255)
                        aaGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAACCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCa                                                                                                                            <  2:6205510/150‑1 (MQ=255)
                             gCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGatat                                                                                                                       >  1:2101885/1‑150 (MQ=255)
                               aGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATcc                                                                                                                     >  2:6680218/1‑150 (MQ=255)
                                   tgtgCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCt                                                                                                                 <  1:1173766/150‑1 (MQ=255)
                                    gtgCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCtt                                                                                                                <  2:4537559/150‑1 (MQ=255)
                                      gCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGt                                                                                                              >  2:3430043/1‑150 (MQ=255)
                                       cAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTa                                                                                                             <  1:3439916/150‑1 (MQ=255)
                                        aGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAg                                                                                                            <  1:5841025/150‑1 (MQ=255)
                                            cGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCtt                                                                                                        <  2:5791396/150‑1 (MQ=255)
                                            cGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCtt                                                                                                        >  1:571670/1‑150 (MQ=255)
                                              aGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTAt                                                                                                      <  2:1916278/150‑1 (MQ=255)
                                               ggTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATg                                                                                                     <  2:1306254/150‑1 (MQ=255)
                                               ggTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATg                                                                                                     <  1:5915029/150‑1 (MQ=255)
                                               ggTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATg                                                                                                     <  1:5357477/150‑1 (MQ=255)
                                                   gATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAg                                                                                                 >  1:520801/1‑150 (MQ=255)
                                                     tGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAggg                                                                                               >  2:2287054/1‑150 (MQ=255)
                                                         aaGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACt                                                                                           >  2:5479326/1‑150 (MQ=255)
                                                           gCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGa                                                                                         >  2:4115415/1‑150 (MQ=255)
                                                                ccGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATgg                                                                                    <  1:6429325/150‑1 (MQ=255)
                                                                ccGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATgg                                                                                    <  1:721196/150‑1 (MQ=255)
                                                                ccGGCTGGAACGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCAATGGTGCTGTGATTTGGGGCCGCTTTGCGGCCACAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATgg                                                                                    >  2:2814145/1‑150 (MQ=255)
                                                                 cGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGc                                                                                   <  1:4080632/150‑1 (MQ=255)
                                                                    cTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGTCACTGAAATGGCCCa                                                                                <  2:3770332/150‑1 (MQ=255)
                                                                       gAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGa                                                                             >  2:4959690/1‑150 (MQ=255)
                                                                        aaGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGaa                                                                            <  1:4536342/150‑1 (MQ=255)
                                                                          ggCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAAct                                                                          >  2:1694625/1‑150 (MQ=255)
                                                                          ggCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAAct                                                                          <  2:1627569/150‑1 (MQ=255)
                                                                           gCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAActc                                                                         >  1:4952711/1‑150 (MQ=255)
                                                                            cATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAActct                                                                        <  1:37753/150‑1 (MQ=255)
                                                                            cATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTGATGGGAGGGCACTGAAATGGCCCAGGAActct                                                                        >  1:4369272/1‑150 (MQ=255)
                                                                             aTTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTa                                                                       <  2:1236927/150‑1 (MQ=255)
                                                                             aTTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTa                                                                       >  1:2779352/1‑150 (MQ=255)
                                                                              ttCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTac                                                                      <  1:2123185/150‑1 (MQ=255)
                                                                              ttCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTac                                                                      <  1:2190503/150‑1 (MQ=255)
                                                                              ttCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTac                                                                      >  2:5250273/1‑150 (MQ=255)
                                                                                 tACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACAcc                                                                   >  2:1420214/1‑150 (MQ=255)
                                                                                    gacgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCttt                                                                                                                                                    <  1:29408/66‑1 (MQ=255)
                                                                                    gacgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAg                                                                               >  2:2409562/1‑135 (MQ=255)
                                                                                     acgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTa                                                               <  1:6017818/150‑1 (MQ=255)
                                                                                      cgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTac                                                              <  2:1446290/150‑1 (MQ=255)
                                                                                       ggCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTacc                                                             >  1:4662044/1‑150 (MQ=255)
                                                                                          aaTGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTaccaac                                                          <  1:5537522/150‑1 (MQ=255)
                                                                                          aaTGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTaccaac                                                          >  1:5592740/1‑150 (MQ=255)
                                                                                                tGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGaaa                                                    >  1:4708283/1‑150 (MQ=255)
                                                                                                tGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGaaa                                                    >  2:586666/1‑150 (MQ=255)
                                                                                                tGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGaaa                                                    <  1:6754577/150‑1 (MQ=255)
                                                                                                 ggAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAAc                                                   <  2:4179027/150‑1 (MQ=255)
                                                                                                   aaGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTg                                                 <  2:4057909/150‑1 (MQ=255)
                                                                                                     ggTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGtt                                               <  2:3904768/150‑1 (MQ=255)
                                                                                                     ggTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGtt                                               <  2:1347749/150‑1 (MQ=255)
                                                                                                       ttGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGttct                                             >  2:6596803/1‑150 (MQ=255)
                                                                                                         gAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGttcttc                                           >  2:4433302/1‑150 (MQ=255)
                                                                                                          aaGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCg                                          >  2:3255547/1‑150 (MQ=255)
                                                                                                                aGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGcc                                    <  1:2926210/150‑1 (MQ=255)
                                                                                                                aGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGcc                                    <  1:665189/150‑1 (MQ=255)
                                                                                                                  cgcTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTc                                  <  2:590655/150‑1 (MQ=255)
                                                                                                                   gcTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCg                                 >  1:2418559/1‑150 (MQ=255)
                                                                                                                       aTCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCt                             >  1:1324803/1‑150 (MQ=255)
                                                                                                                        tCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTc                            <  2:5932754/150‑1 (MQ=255)
                                                                                                                        tCCATTGGTGCTGTGATGTGGGGCCGCTTTGCGGCCCCAATCATTTATGAGGCAGAGATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAAGGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTc                            <  2:720253/150‑1 (MQ=255)
                                                                                                                          cATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGa                          <  2:883644/150‑1 (MQ=255)
                                                                                                                          cATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGa                          <  1:4020125/150‑1 (MQ=255)
                                                                                                                           aTTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGAAAACCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCAAGTAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAg                         >  1:4873408/1‑150 (MQ=255)
                                                                                                                                tGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCAt                    <  2:2663846/150‑1 (MQ=255)
                                                                                                                                 gCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTGGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATg                   <  2:1355455/150‑1 (MQ=255)
                                                                                                                                 gCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATg                   <  1:4479389/150‑1 (MQ=255)
                                                                                                                                  cTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATgg                  >  2:5608837/1‑150 (MQ=255)
                                                                                                                                  cTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATgg                  <  2:936288/150‑1 (MQ=255)
                                                                                                                                  cTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATgg                  >  2:1212722/1‑150 (MQ=255)
                                                                                                                                  cTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATgg                  >  2:1745831/1‑150 (MQ=255)
                                                                                                                                  cTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATgg                  >  2:3003305/1‑150 (MQ=255)
                                                                                                                                      gATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCaa              <  2:3112343/150‑1 (MQ=255)
                                                                                                                                         ttGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTCGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGc           <  2:2382109/150‑1 (MQ=255)
                                                                                                                                         ttGGGGCCGCAAAGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGttc                                              >  1:1686233/1‑115 (MQ=255)
                                                                                                                                              gCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAAc      >  1:3236674/1‑150 (MQ=255)
                                                                                                                                              gCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAAc      >  2:3498981/1‑150 (MQ=255)
                                                                                                                                               ccGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACa     >  1:1456324/1‑150 (MQ=255)
                                                                                                                                                 gCTTTGCGGCCCCAATCATTTATGATTCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGcc                                        <  1:2047298/113‑1 (MQ=255)
                                                                                                                                                 gCTTTGCGGCCCCAATCATTTATGATCCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGcc                                        >  2:2047298/1‑113 (MQ=255)
                                                                                                                                                  cTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACAAAg  <  1:838780/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GTGATCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACAAAG  >  NC_002947/2448345‑2448640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑