BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,779,804	G→A	5.1%	P234S (CCA→TCA)	PP_5521 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,779,804	0	G	A	5.1%	885.1 / 21.7	275	P234S (CCA→TCA)	PP_5521	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (135/126); new base A (10/4); total (145/130)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

GTCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCAACCCCT  >  NC_002947/2779655‑2779953

gtCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTgg                                                                                                                                                        <  1:6163119/150‑1 (MQ=255)

 tCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGgc                                                                                                                                                       >  1:5466017/1‑150 (MQ=255)

 tCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGgc                                                                                                                                                       <  1:5569504/150‑1 (MQ=255)

    aGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGgcagc                                                                                                                                                    <  1:5390785/150‑1 (MQ=255)

    aGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGgcagc                                                                                                                                                    >  2:4264014/1‑150 (MQ=255)

    aGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGgcagc                                                                                                                                                    >  1:5073170/1‑150 (MQ=255)

    aGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGgcagc                                                                                                                                                    >  1:6817651/1‑150 (MQ=255)

      aaCAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGcagcag                                                                                                                                                  <  1:230885/150‑1 (MQ=255)

        cAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGc                                                                                                                                                <  1:6557721/150‑1 (MQ=255)

         aaGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCg                                                                                                                                               >  2:4331855/1‑150 (MQ=255)

          aGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCgc                                                                                                                                              <  2:5376639/150‑2 (MQ=255)

          aGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGa                                                                                                                                              <  2:4703322/150‑1 (MQ=255)

          aGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCACTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGa                                                                                                                                              >  1:4089631/1‑150 (MQ=255)

            aTGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGt                                                                                                                                            >  1:3256812/1‑150 (MQ=255)

            aTGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGCGCCTGCTGCCACAGCAGGcacgc                                                                                                                                            <  1:48282/150‑5 (MQ=255)

               tCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGc                                                                                                                                         <  2:6238954/150‑1 (MQ=255)

                 ggCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCAc                                                                                                                                       >  1:2170302/1‑150 (MQ=255)

                 ggCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCAc                                                                                                                                       >  1:1470718/1‑150 (MQ=255)

                      ccTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGa                                                                                                                                  >  2:1083221/1‑150 (MQ=255)

                      ccTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGa                                                                                                                                  <  1:1204576/150‑1 (MQ=255)

                           cTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                             <  2:6106296/150‑1 (MQ=255)

                            tCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGcc                                                                                                                            <  1:870366/150‑1 (MQ=255)

                             cACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCa                                                                                                                           <  1:697574/150‑1 (MQ=255)

                               cccACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGt                                                                                                                         <  1:6784184/150‑1 (MQ=255)

                               cccACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGt                                                                                                                         <  2:4078846/150‑1 (MQ=255)

                                  acgacgATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACg                                                                                                                      >  2:4529815/1‑150 (MQ=255)

                                    gacgaTCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGaa                                                                                                                    <  1:1102503/150‑1 (MQ=255)

                                    gacgaTCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGaa                                                                                                                    <  1:5832612/150‑1 (MQ=255)

                                    gacgaTCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGaa                                                                                                                    <  2:6055849/150‑1 (MQ=255)

                                       gaTCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGAt                                                                                                                 >  2:4075486/1‑150 (MQ=255)

                                        aTCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATa                                                                                                                <  2:505983/150‑1 (MQ=255)

                                            gCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCtt                                                                                                            <  2:2020822/150‑1 (MQ=255)

                                             ccGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTc                                                                                                           >  2:1610827/1‑150 (MQ=255)

                                             ccGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTc                                                                                                           <  1:900398/150‑1 (MQ=255)

                                             ccGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTc                                                                                                           <  1:3149679/150‑1 (MQ=255)

                                               ggCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCgg                                                                                                         <  1:6019575/150‑1 (MQ=255)

                                                gCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGgc                                                                                                        <  2:3747444/150‑1 (MQ=255)

                                                    tCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGGTATCTTCgtactgg                                                                                                    <  1:3940201/150‑7 (MQ=255)

                                                      cgccgACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCAc                                                                                                  <  2:3479703/150‑1 (MQ=255)

                                                       gccgACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCAcc                                                                                                 <  2:5929246/150‑1 (MQ=255)

                                                           acacCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACcagc‑a                                                                                            <  1:1865764/150‑1 (MQ=255)

                                                           acacCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACcagc‑a                                                                                            <  2:3368853/150‑1 (MQ=255)

                                                             acCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGt                                                                                          >  1:172828/1‑150 (MQ=255)

                                                                 cACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTc                                                                                                           >  1:833498/1‑130 (MQ=255)

                                                                 cACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTc                                                                                      <  1:4529815/150‑1 (MQ=255)

                                                                     atgctccatgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTg                                                                                  <  2:299805/150‑1 (MQ=255)

                                                                     atgctccatgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTg                                                                                  <  1:4231754/150‑1 (MQ=255)

                                                                     atgctccatgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTg                                                                                  <  1:2579552/150‑1 (MQ=255)

                                                                        ctccatgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGcc                                                                               <  2:2107091/150‑1 (MQ=255)

                                                                         tccatgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCGAGTACT‑GCTggtga                                                                              <  1:1614244/150‑4 (MQ=255)

                                                                           catgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATc                                                                            >  1:6227040/1‑150 (MQ=255)

                                                                           catgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATc                                                                            <  2:6394127/150‑1 (MQ=255)

                                                                             tgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCgg                                                                          <  1:460562/150‑1 (MQ=255)

                                                                                  aGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCGTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAg                                                                     <  1:5514507/150‑1 (MQ=255)

                                                                                   gCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAgg                                                                    <  1:2845019/150‑1 (MQ=255)

                                                                                     cccAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAgggg                                                                  <  2:6346821/150‑1 (MQ=255)

                                                                                       cAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGAt                                                                <  2:1522787/150‑1 (MQ=255)

                                                                                        aGGCTGATGTCGTCCGGGTCGATGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATGCCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATc                                                               >  2:2327171/1‑150 (MQ=255)

                                                                                         ggCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCa                                                              <  2:2619937/150‑1 (MQ=255)

                                                                                         ggCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCa                                                              <  2:4414656/150‑1 (MQ=255)

                                                                                            tGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCCTCGGGGAAGGGGATCAgcg                                                           >  2:417855/1‑150 (MQ=255)

                                                                                               tGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGcgg                                                        >  2:4805798/1‑150 (MQ=255)

                                                                                                gtcgtcCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGcggt                                                       <  1:5149112/150‑1 (MQ=255)

                                                                                                  cgtcCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGcggtcg                                                     <  1:3713038/150‑1 (MQ=255)

                                                                                                        ggTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTgg                                               <  1:33137/150‑1 (MQ=255)

                                                                                                              tGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCa                                         >  2:752318/1‑150 (MQ=255)

                                                                                                                 ccTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGt                                      >  2:3892887/1‑150 (MQ=255)

                                                                                                                 ccTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGt                                      >  1:6331073/1‑150 (MQ=255)

                                                                                                                  cTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTc                                     >  1:2469705/1‑150 (MQ=255)

                                                                                                                     ggCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGaa                                  <  2:6548672/150‑1 (MQ=255)

                                                                                                                      gCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAAc                                 >  1:4644559/1‑150 (MQ=255)

                                                                                                                      gCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCACTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAAc                                 >  2:6763082/1‑150 (MQ=255)

                                                                                                                       ccAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGACGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACg                                >  2:4107833/1‑150 (MQ=255)

                                                                                                                       ccAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACg                                >  1:6634558/1‑150 (MQ=255)

                                                                                                                        cAGCTCTTCGAGCACGAGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGATTGGCCAGTACGAAGATACCTTCTGCGCTAACCAGC‑AGTACTCGCTGTCCATAGGGGAAGGGGATCAGCGCGGTCGGTGTGTGCGGCAGGTCGAACgg                               >  2:3907345/1‑150 (MQ=255)

                                                                                                                          gCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCt                             >  2:5605503/1‑150 (MQ=255)

                                                                                                                          gCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCt                             <  2:1009230/150‑1 (MQ=255)

                                                                                                                           ctctTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTc                            <  1:1334580/150‑1 (MQ=255)

                                                                                                                           ctctTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTc                            <  1:3636943/150‑1 (MQ=255)

                                                                                                                              ttCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGaa                         >  1:4300983/1‑150 (MQ=255)

                                                                                                                              ttCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGaa                         >  1:1965184/1‑150 (MQ=255)

                                                                                                                                cGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACg                       >  1:3857324/1‑150 (MQ=255)

                                                                                                                                 gAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACgg                      >  1:2023127/1‑150 (MQ=255)

                                                                                                                                   gCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGt                    >  2:95889/1‑150 (MQ=255)

                                                                                                                                     aCGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTaa                  >  1:4972661/1‑150 (MQ=255)

                                                                                                                                     aCGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTaa                  >  2:3215781/1‑150 (MQ=255)

                                                                                                                                      gactcgCCTGCTGCCACAGCAGGCAAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGt                                      <  1:779748/124‑1 (MQ=255)

                                                                                                                                         tcgCCTGCTGCCACAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTg              >  1:6492472/3‑150 (MQ=255)

                                                                                                                                           gCCTGCTGCCACAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTggc            >  2:1415760/1‑150 (MQ=255)

                                                                                                                                            ccTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTggcg           <  2:798629/150‑1 (MQ=255)

                                                                                                                                             ctgctgTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTggcgg          >  2:5266503/1‑150 (MQ=255)

                                                                                                                                             ctgctgTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTggcgg          <  2:5699974/150‑1 (MQ=255)

                                                                                                                                              tgctgTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTggcggc         >  2:1771581/1‑150 (MQ=255)

                                                                                                                                                ctgTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCaa       <  1:56597/150‑1 (MQ=255)

                                                                                                                                                ctgTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCaa       >  2:3306817/1‑150 (MQ=255)

                                                                                                                                                ctgTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCaa       <  1:964540/150‑1 (MQ=255)

                                                                                                                                                ctgTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCaa       >  2:2823357/1‑150 (MQ=255)

                                                                                                                                                   tgGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCAAccc    >  2:309215/1‑150 (MQ=255)

                                                                                                                                                     gcagcaGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCAACCCCt  >  1:3078794/1‑150 (MQ=255)

                                                                                                                                                     gcagcaGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCAACCCCt  >  2:866619/1‑150 (MQ=255)

GTCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCAACCCCT  >  NC_002947/2779655‑2779953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`