BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,297,378	C→T	5.8%	P557L (CCG→CTG)	PP_2893 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,297,378	0	C	T	5.8%	987.8 / 30.4	311	P557L (CCG→CTG)	PP_2893	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (145/148); new base T (12/6); total (157/154)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 332 total aligned reads displayed.

GGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGACACAGCCTTGG  >  NC_002947/3297229‑3297527

gggTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCCGCCCCCGGTGGGCCAGGCTAC‑AAAGCCCGGTCAAGGGCCGGCGGGATGTGGCCGGCACCATGCAGGcccc                                                                                                                                                        >  1:3645905/1‑150 (MQ=255)

  gTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AATGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCATGCAGGCCCCGc                                                                                                                                                      >  2:854224/1‑150 (MQ=255)

       aaCCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCACCGGTGGCCGAGGCTAC‑AAGGCCCGGTCAAGGGCCGGCTGGATGTCGCCGGCACCATGCAGGCCCCCCAAGGt                                                                                                                                                 >  2:2483435/1‑150 (MQ=255)

         ccGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCCGCGGTGGCCGAGGCTAC‑AAGGCCGGGTGAAGGGCCGGCGGGGTGTCGCCGGCACCTTGCAGGCCCCGCAAGGTAc                                                                                                                                               >  2:2295801/1‑150 (MQ=255)

         ccGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCTGTGGCCGCGGCGAC‑AAGGCCGGGTCAAGGGCCGGCTGGCTGTCGCCGGCACCGTGCAGGCCCCCCCAGGTAc                                                                                                                                               >  2:3203742/1‑150 (MQ=255)

          cGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCACGGCCAGCTGTGGCCGAGGCTAC‑AACGCCCGGTCAAGGGCCGGCTGGATTTCGCCGGCACCGTGCAGGCCCCCCAAGGTACg                                                                                                                                              >  1:6703626/1‑150 (MQ=255)

            cATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGGCGAGGCCCGCAGTGGCCGAGGCTACAAAGGCCGGG‑GAACGGCCCGCTGGATGTCGCCGGCACCCTGCACCCCCCCCAAGCGACGcg                                                                                                                                            >  1:3435782/1‑149 (MQ=255)

            cATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCt                                                                                                                                            >  2:3372658/1‑150 (MQ=255)

                       gTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAgg                                                                                                                                 <  2:1252631/150‑1 (MQ=255)

                             gCTTGCAACAGCGCCTGGCTGGGCGACTCGCCCTTGCCGGGCCGCGCCTCGGCCAGCGGGGGCCGAGGCTAC‑CCGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑Agc                                                                                                                          <  1:6584310/150‑1 (MQ=255)

                                tGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCTGGCCCTGCAGGGTGAGCGTACCTTGCGGGGCC‑Tgcaag                                                                                                                       <  2:6805535/150‑4 (MQ=37)

                                 gCAACAGCGCCGGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCtt                                                                                                                      <  2:6631991/150‑1 (MQ=255)

                                  cAACAGCGCCTGGCTGGGCGACTCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTGGCCCTGCAGGGTG‑Agcgtacc                                                                                                                     <  1:1972147/150‑5 (MQ=255)

                                  cAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTg                                                                                                                     <  1:2628176/150‑1 (MQ=255)

                                   aaCCGCGCCTGGCTGGGCGAATCGACCTTGACGTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTgg                                                                                                                    <  1:5000089/150‑1 (MQ=255)

                                   aaCAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTgg                                                                                                                    <  1:2925576/150‑1 (MQ=255)

                                   aaCAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCGGGCTGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑CGCGCTTgg                                                                                                                    >  2:4992014/1‑150 (MQ=255)

                                   aaCAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCCCCAGTGGCCGAGGCTAC‑AAGGCCGGGGCAAGGGCCGGCTGGATTTAGCCCGCACCATGCAGGCCCCCCACGGTACGCACACCCGGCAGGGCC‑CCCGCtttg                                                                                                                    >  1:4410006/1‑148 (MQ=255)

                                    aCAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTTGCCGCGGCGAC‑AAGGCCGGGGCAAGGGCCGGCGGGTTGTCGCCGGCACCTTGCCGGCCCCGCCAGGTACGCTCACCCGGCCGGGCC‑CGCGCGTGGc                                                                                                                   >  1:2752041/1‑150 (MQ=255)

                                    aCAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGGTGTCGCCGGCACCATGCCGGCCCCCCACGGTCCGCTCCCCCTGCCGGGCC‑AGCGCGTGGc                                                                                                                   >  2:4271810/1‑150 (MQ=255)

                                       gcgcCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCa                                                                                                                <  1:469122/150‑1 (MQ=255)

                                        cgcCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAg                                                                                                               <  1:6500106/150‑1 (MQ=255)

                                         gcCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAgg                                                                                                              <  2:6705017/150‑1 (MQ=255)

                                          ccTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCCAAGCGC‑TGGccctgca                                                                                                             <  1:3502681/150‑5 (MQ=255)

                                                    cGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGc                                                                                                   <  1:5351454/150‑1 (MQ=255)

                                                    cGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGc                                                                                                   <  2:1166902/150‑1 (MQ=255)

                                                       agcGACCGTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTg                                                                                                <  1:326223/148‑1 (MQ=255)

                                                        tCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTgc                                                                                               <  1:3863867/150‑1 (MQ=255)

                                                             cTTGACTTGCCGCGCCTCGGCCACCGGTGGCCGAGGCTAC‑AAGGCCCGGTCAAGGGCCGCCTGGATGTCGCCGGCACCATGCACGCCCCCCCACGTAACCACACCCAGCAGGGCC‑ACCCCTTGGCCCAGGCCGAAAACCCCcagcagcag                                                                                          >  2:2406195/1‑150 (MQ=255)

                                                              gtGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGt                                                                                         <  2:5684286/149‑1 (MQ=255)

                                                               tGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGtt                                                                                        <  1:2041560/150‑1 (MQ=255)

                                                                     gCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCt                                                                                  <  1:2150550/150‑1 (MQ=255)

                                                                        gcgcCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGAAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGa                                                                               >  2:4373714/1‑150 (MQ=255)

                                                                           ccTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGc                                                                            <  1:415045/150‑1 (MQ=255)

                                                                           ccTCGGCCACAGCTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAg                                                                                                                                                   <  2:6217828/80‑1 (MQ=38)

                                                                            cTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGcc                                                                           >  1:6087767/1‑150 (MQ=255)

                                                                                gCCAGGTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGcc                                                                       <  1:1486599/150‑1 (MQ=255)

                                                                                 ccAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCt                                                                      >  2:5136863/1‑150 (MQ=255)

                                                                                 ccAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCt                                                                      <  1:3093285/150‑1 (MQ=255)

                                                                                 ccAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCt                                                                      <  2:2652067/150‑1 (MQ=255)

                                                                                 ccAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCGTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCt                                                                      <  1:1131763/150‑1 (MQ=255)

                                                                                        tgGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAAc                                                               <  1:3167698/150‑1 (MQ=255)

                                                                                        tgGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAAc                                                               >  1:1760405/1‑150 (MQ=255)

                                                                                          gCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGc                                                             >  2:3669643/1‑150 (MQ=255)

                                                                                          gCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGc                                                             >  1:4933891/1‑150 (MQ=255)

                                                                                           ccGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGcc                                                            >  2:886325/1‑150 (MQ=255)

                                                                                              aGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCaa                                                         >  1:5697904/1‑150 (MQ=255)

                                                                                               ggCTAC‑AAGGCCAGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAAc                                                        >  1:5263450/1‑150 (MQ=255)

                                                                                                 cTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAAcgc                                                      <  2:3977448/150‑1 (MQ=255)

                                                                                                  tAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAAcgcg                                                     >  1:553320/1‑150 (MQ=255)

                                                                                                  tAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAAcgcg                                                     >  1:1838296/1‑150 (MQ=255)

                                                                                                  tAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAAcgcg                                                     >  1:258567/1‑150 (MQ=255)

                                                                                                  tAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGAGGCCCGCCTGGACAACGCCCAAcgcg                                                     >  1:3976151/1‑150 (MQ=255)

                                                                                                   aC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCgg                                                    >  2:4583398/1‑150 (MQ=255)

                                                                                                        ggCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTa                                                <  1:4541688/150‑1 (MQ=255)

                                                                                                         gCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAg                                               >  2:4881170/1‑150 (MQ=255)

                                                                                                         gCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAg                                               >  2:6390986/1‑150 (MQ=255)

                                                                                                          ccGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGt                                              >  2:6490645/1‑150 (MQ=255)

                                                                                                           cGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGcc                                                                                                  <  2:4032980/97‑1 (MQ=255)

                                                                                                           cGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTc                                             <  2:3613694/150‑1 (MQ=255)

                                                                                                           cGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTc                                             <  2:1785268/150‑1 (MQ=255)

                                                                                                            gggTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGcc                                                            >  2:2923063/1‑134 (MQ=255)

                                                                                                             ggTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGa                                           <  1:931554/150‑1 (MQ=255)

                                                                                                             ggTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGa                                           <  1:627563/150‑1 (MQ=255)

                                                                                                             ggTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGa                                           <  1:2444768/150‑1 (MQ=255)

                                                                                                                cAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCt                                        <  2:2107733/150‑1 (MQ=255)

                                                                                                                 aaGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTc                                       >  1:5439492/1‑150 (MQ=255)

                                                                                                                   gggCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCaa                                     <  2:3535500/150‑1 (MQ=255)

                                                                                                                    ggCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCTCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAg                                    <  1:4738586/150‑1 (MQ=255)

                                                                                                                    ggCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCagg                                    <  1:1293562/150‑3 (MQ=255)

                                                                                                                    ggCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAg                                    <  1:4738522/150‑1 (MQ=255)

                                                                                                                    ggCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAg                                    <  2:1987328/150‑1 (MQ=255)

                                                                                                                      ccGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGc                                  >  2:4167807/1‑150 (MQ=255)

                                                                                                                             gATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGt                           >  1:2403339/1‑150 (MQ=255)

                                                                                                                               tGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCaa                                     <  1:5748616/138‑1 (MQ=255)

                                                                                                                               tGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTAt                         >  2:5595054/1‑150 (MQ=255)

                                                                                                                               tGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTAt                         <  1:1406217/150‑1 (MQ=255)

                                                                                                                                 tCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATcc                       >  2:54537/1‑150 (MQ=255)

                                                                                                                                 tCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATcc                       <  2:4735883/150‑1 (MQ=255)

                                                                                                                                   gCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCAc                     <  1:6199225/150‑1 (MQ=255)

                                                                                                                                      cgtaCCTTGCGGGGCCTGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTg                  >  1:6361645/4‑150 (MQ=255)

                                                                                                                                       gtaCCTTGCGGGGCCTGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTgg                 >  1:718173/3‑150 (MQ=255)

                                                                                                                                        taCCTTGCGGGGCCTGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGt                           >  2:1857161/2‑139 (MQ=255)

                                                                                                                                         aCCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTCTCCACCTGGGt               >  2:1948636/1‑150 (MQ=255)

                                                                                                                                         aCCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGt               <  2:939014/150‑1 (MQ=255)

                                                                                                                                         aCCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGt               <  2:5839233/150‑1 (MQ=255)

                                                                                                                                           cTTGCGGGGCCTGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGa             >  1:25151/1‑150 (MQ=255)

                                                                                                                                           cTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGa             >  1:157972/1‑150 (MQ=255)

                                                                                                                                            ttGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGac            >  1:4554145/1‑150 (MQ=255)

                                                                                                                                            ttGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGac            >  1:3847704/1‑150 (MQ=255)

                                                                                                                                              gCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGacac          >  2:10461/1‑150 (MQ=255)

                                                                                                                                              gCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGacac          >  1:5098734/1‑150 (MQ=255)

                                                                                                                                              gCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGacac          >  1:2772000/1‑150 (MQ=255)

                                                                                                                                                aGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGa                                                                   <  2:5256498/91‑1 (MQ=255)

                                                                                                                                                aGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGACACAg        >  1:6221862/1‑150 (MQ=255)

                                                                                                                                                    cccGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGACACAGCCtt    >  2:4481445/1‑150 (MQ=255)

                                                                                                                                                     ccGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGACACAGCCTTg   <  1:487177/150‑1 (MQ=255)

                                                                                                                                                      cGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGACAACCGCCTGCTGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCATGGCAACCGGTATCCACCTGGGTGACACAGCCTTgg  <  1:5580121/150‑1 (MQ=255)

                                                                                                                                                      cGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGACACAGCCTTgg  >  2:1598973/1‑150 (MQ=255)

                                                                                                                                                      cGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGACACAGCCTTgg  <  1:2664249/150‑1 (MQ=255)

GGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTAC‑AAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACAACGCCCAACGCGGTGTAGTCGAGCTCAAGGCAACCGGTATCCACCTGGGTGACACAGCCTTGG  >  NC_002947/3297229‑3297527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`