Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,640,692 A→T M20K (ATG→AAG)  uvrY ← BarA/UvrY two‑componenent system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,640,6920AT99.7% 1150.6 / NA 321M20K (ATG→AAG) uvrYBarA/UvrY two‑componenent system response regulator
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (144/176);  total (145/176)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 321 total aligned reads displayed.

GTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAAC  >  NC_002947/4640546‑4640838
                                                                                                                                                  |                                                                                                                                                  
gTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTcc                                                                                                                                                 <  1:2933589/150‑1 (MQ=255)
 tttGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCg                                                                                                                                                >  2:2242963/1‑150 (MQ=255)
 tttGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCg                                                                                                                                                >  2:1843578/1‑150 (MQ=255)
 tttGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCg                                                                                                                                                >  1:5410414/1‑150 (MQ=255)
  ttGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCgt                                                                                                                                               <  2:3218759/150‑1 (MQ=255)
  ttGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCgt                                                                                                                                               <  1:1726680/150‑1 (MQ=255)
  ttGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATATTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCgt                                                                                                                                               <  2:6693148/150‑1 (MQ=255)
       ggTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAAt                                                                                                                                          >  2:4408240/1‑150 (MQ=255)
       ggTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAAt                                                                                                                                          >  1:8318/1‑150 (MQ=255)
         tGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATAc                                                                                                                                        <  1:188878/150‑1 (MQ=255)
           gCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCg                                                                                                                                      >  2:6905845/1‑150 (MQ=255)
            ccTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCgg                                                                                                                                     <  2:2637273/150‑1 (MQ=255)
            ccTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCg                                                                                                                                      >  1:2485131/1‑149 (MQ=255)
             cTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGt                                                                                                                                    <  2:4862627/150‑1 (MQ=255)
              tCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCgt                                                                                                                                               >  1:1387458/1‑138 (MQ=255)
              tCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGtt                                                                                                                                   <  2:5931192/150‑1 (MQ=255)
               ccAGGCCGCCGATGCCGGGCATTTTCACCTCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTc                                                                                                                                  <  2:2175989/150‑1 (MQ=255)
               ccAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTc                                                                                                                                  <  1:4313461/150‑1 (MQ=255)
               ccAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTc                                                                                                                                  >  1:4813134/1‑150 (MQ=255)
               ccAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTc                                                                                                                                  <  1:2242963/150‑1 (MQ=255)
                cAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCg                                                                                                                                 >  2:5873183/1‑150 (MQ=255)
                 aGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGt                                                                                                                                <  1:7976972/150‑1 (MQ=255)
                   gccgccGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTAc                                                                                                                              >  2:3548533/1‑150 (MQ=255)
                       ccgATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGa                                                                                                                          <  2:7157432/150‑1 (MQ=255)
                         gATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATc                                                                                                                        >  1:2197637/1‑150 (MQ=255)
                         gATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATc                                                                                                                        <  2:6840410/150‑1 (MQ=255)
                             ccGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGa                                                                                                                    <  1:6447249/150‑1 (MQ=255)
                               gggCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCTGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGa                                                                                                                    <  2:6364964/148‑1 (MQ=255)
                               gggCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCgtgt                                                                                                                                             <  1:5171376/123‑1 (MQ=255)
                               gggCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGatc                                                                                                                  >  2:495822/1‑150 (MQ=255)
                               gggCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGatc                                                                                                                  >  1:3977381/1‑150 (MQ=255)
                               gggCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGatc                                                                                                                  >  2:6006280/1‑150 (MQ=255)
                               gggCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGatc                                                                                                                  >  2:7555258/1‑150 (MQ=255)
                                ggCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGatca                                                                                                                 <  1:1068091/150‑1 (MQ=255)
                                  cATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGatcatc                                                                                                               <  2:251218/150‑1 (MQ=255)
                                  cATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGatcatc                                                                                                               <  1:8122861/150‑1 (MQ=255)
                                     tttCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGAc                                                                                                            <  1:2560556/150‑1 (MQ=255)
                                      ttCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGAcc                                                                                                           <  2:3286475/150‑1 (MQ=255)
                                        cacaTCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCAc                                                                                                         <  1:4447667/150‑1 (MQ=255)
                                         acaTCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCAGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACt                                                                                                        <  2:5401214/150‑1 (MQ=255)
                                          catccatcAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTa                                                                                                       >  1:1962070/1‑150 (MQ=255)
                                           atccatcAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTCATACCGGTTCGTACCAGATCGTGATCATCGACCACTaa                                                                                                      <  1:1276665/150‑1 (MQ=255)
                                           atccatcAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGat                                                                                                                   <  2:100520/137‑1 (MQ=255)
                                           atccatcAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGat                                                                                                                   >  1:100520/1‑137 (MQ=255)
                                           atccatcAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTaa                                                                                                      <  2:1238630/150‑1 (MQ=255)
                                            tccatcAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGa                                                                                                                    >  2:3642263/1‑135 (MQ=255)
                                                   gCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTaa                                                                                              <  1:3735328/150‑1 (MQ=255)
                                                     acaACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATc                                                                                            <  2:3435660/150‑1 (MQ=255)
                                                       aaCGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCaa                                                                                          >  2:1913870/1‑150 (MQ=255)
                                                        aCGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAg                                                                                         <  1:4751083/150‑1 (MQ=255)
                                                           tCCGGTTTCAGCTCCCGGGCCAGTTTCGGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAg                                                                                         >  2:1024848/1‑147 (MQ=255)
                                                           tCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGcac                                                                                      >  1:2295911/1‑150 (MQ=255)
                                                           tCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGcac                                                                                      >  2:7458652/1‑150 (MQ=255)
                                                               gTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCacacac                                                                                  <  1:5061211/150‑1 (MQ=255)
                                                                  tCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTc                                                                               <  1:7287399/150‑1 (MQ=255)
                                                                    aGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGt                                                                             <  2:7699156/150‑1 (MQ=255)
                                                                      cTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTca                                                                           <  1:6286678/150‑1 (MQ=255)
                                                                         ccGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGatcatc                                                                                                               <  1:7816326/111‑1 (MQ=255)
                                                                           gggCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCCCCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAgg                                                                      <  2:1468793/150‑1 (MQ=255)
                                                                              ccAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCg                                                                   >  2:7305764/1‑150 (MQ=255)
                                                                              ccAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCg                                                                   >  1:7670912/1‑150 (MQ=255)
                                                                              ccAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGAACACACCTCGTCAGCAGGGCg                                                                   >  2:2891633/1‑150 (MQ=255)
                                                                                aGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGAt                                                                 >  2:1369333/1‑150 (MQ=255)
                                                                                 gTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGAtt                                                                <  2:6454502/150‑1 (MQ=255)
                                                                                 gTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGAtt                                                                >  1:1457199/1‑150 (MQ=255)
                                                                                     cAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGAt                                                            <  2:4527931/150‑1 (MQ=255)
                                                                                        tGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATccc                                                         <  1:740998/150‑1 (MQ=255)
                                                                                            gACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCa                                                     <  2:5100375/150‑1 (MQ=255)
                                                                                               tCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCt                                                  <  2:1457199/150‑1 (MQ=255)
                                                                                               tCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCt                                                  >  1:6204266/1‑150 (MQ=255)
                                                                                               tCGCCGGAATCTCACTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCt                                                  >  1:1752047/1‑150 (MQ=255)
                                                                                                          tcCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAAttt                                       >  1:2061878/1‑150 (MQ=255)
                                                                                                            ccTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAAttttt                                     <  2:7862578/150‑1 (MQ=255)
                                                                                                            ccTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAAttt                                       >  2:4810777/1‑148 (MQ=255)
                                                                                                             cTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGAtt                                                                <  2:3807885/122‑1 (MQ=255)
                                                                                                             cTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTc                                    <  1:6813257/150‑1 (MQ=255)
                                                                                                             cTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTc                                    >  2:3367230/1‑150 (MQ=255)
                                                                                                               cacccaccACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCAc                                  <  1:3115463/150‑1 (MQ=255)
                                                                                                                acccaccACCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACg                                 >  1:2722967/1‑150 (MQ=255)
                                                                                                                  ccaccaCCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGa                                                             >  1:3803656/1‑120 (MQ=255)
                                                                                                                  ccaccaCCTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGt                               >  1:6517898/1‑150 (MQ=255)
                                                                                                                    accaccTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTgg                             <  1:1843578/150‑1 (MQ=255)
                                                                                                                    accaccTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGAGTGGATCCCGCCACCTTAACACAATTTTTCACGgggg                             >  1:4897546/1‑150 (MQ=255)
                                                                                                                     ccaccTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTggg                            <  2:4329202/150‑1 (MQ=255)
                                                                                                                      caccTGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGc                           <  2:2405485/150‑1 (MQ=255)
                                                                                                                          tGCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTaa                       <  2:568669/150‑1 (MQ=255)
                                                                                                                           gCAGGCCGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAAc                      <  1:3367230/150‑1 (MQ=255)
                                                                                                                                ccGTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCAtt                 <  2:6100762/150‑1 (MQ=255)
                                                                                                                                  gTCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGc               >  2:8192399/1‑150 (MQ=255)
                                                                                                                                   tCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGct              >  1:4542306/1‑150 (MQ=255)
                                                                                                                                   tCGATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGct              <  1:7002801/150‑1 (MQ=255)
                                                                                                                                    catcgacGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGctt             >  1:3587125/7‑150 (MQ=255)
                                                                                                                                    catcgacGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGctt             >  2:4013122/7‑150 (MQ=255)
                                                                                                                                     gATGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGcttc            <  2:5153115/150‑1 (MQ=255)
                                                                                                                                      aTGTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGcttcc           <  1:7244675/150‑1 (MQ=255)
                                                                                                                                        gTCGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGcttcctt         <  1:5943867/150‑1 (MQ=255)
                                                                                                                                          cGGCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCa       <  2:8083068/150‑1 (MQ=255)
                                                                                                                                            gCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGcc                          >  1:7932420/1‑129 (MQ=255)
                                                                                                                                            gCCAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGcc                          <  2:7932420/129‑1 (MQ=255)
                                                                                                                                             ccAACTTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGaa    >  1:1252468/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAAC  >  NC_002947/4640546‑4640838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑