Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,983,564 (C)8→7 coding (30/696 nt) flgH ← flagellar L‑ring protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,983,5570C.99.4% 1758.3 / 0.8 355coding (37/696 nt)flgHflagellar L‑ring protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base . (170/183);  total (172/183)
Fisher's exact test for biased strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 399 total aligned reads displayed.

CGGTCGCTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGCCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCATCTCGTAGG  >  NC_002947/4983409‑4983700
                                                                                                                                                    |                                                                                                                                               
cGGTCGCTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGc                                                                                                                                                 >  2:7454486/1‑149 (MQ=255)
   tCGCTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGcccc                                                                                                                                              >  2:2230027/1‑149 (MQ=255)
   tCGCTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGcccc                                                                                                                                              >  1:7486924/1‑149 (MQ=255)
    cGCTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGccccc                                                                                                                                             <  2:4005369/149‑1 (MQ=255)
        gTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGcccccccag                                                                                                                                         <  1:6430622/149‑3 (MQ=255)
         tACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGcccccccagg                                                                                                                                        <  2:5625735/149‑4 (MQ=255)
          aCAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGcccccccaggg                                                                                                                                       <  2:3195666/149‑5 (MQ=255)
          aCAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTAGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGcccccccaggg                                                                                                                                       >  2:5720207/1‑145 (MQ=255)
              gTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGaa                                                                                                                                  >  1:1328211/1‑149 (MQ=255)
               ttCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGaaa                                                                                                                                 >  2:5908785/1‑149 (MQ=255)
               ttCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGCACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGaaa                                                                                                                                 >  2:546522/1‑149 (MQ=255)
                tCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGaaaa                                                                                                                                <  2:1408874/149‑1 (MQ=255)
                tCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGaaaa                                                                                                                                <  2:6170052/149‑1 (MQ=255)
                tCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGaaaa                                                                                                                                <  2:4490051/149‑1 (MQ=255)
                tCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGaaaa                                                                                                                                <  1:1041137/149‑1 (MQ=255)
                tCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGaaaa                                                                                                                                <  1:6915460/149‑1 (MQ=255)
                     tCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGac                                                                                                                           >  1:2683162/1‑149 (MQ=255)
                     tCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGac                                                                                                                           >  2:1088050/1‑149 (MQ=255)
                      cGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGaca                                                                                                                          <  2:7156515/149‑1 (MQ=255)
                      cGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGaca                                                                                                                          >  2:5130442/1‑149 (MQ=255)
                       gAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGacaa                                                                                                                         <  1:5827256/149‑1 (MQ=255)
                        aaaCCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGacaac                                                                                                                        >  2:3281484/1‑149 (MQ=255)
                             ggCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACg                                                                                                                   <  1:770569/149‑1 (MQ=255)
                               ccTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGAt                                                                                                                 <  2:2976856/149‑1 (MQ=255)
                                cTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGAtt                                                                                                                >  1:196234/1‑149 (MQ=255)
                                  ggTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCa                                                                                                              >  1:85967/1‑149 (MQ=255)
                                     aGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGa                                                                                                           >  1:6553343/1‑149 (MQ=255)
                                      gATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGac                                                                                                          >  2:1224131/1‑149 (MQ=255)
                                      gATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGac                                                                                                          <  2:3072283/149‑1 (MQ=255)
                                      gATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGac                                                                                                          <  2:910349/149‑1 (MQ=255)
                                        tCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGacac                                                                                                        >  1:7378165/1‑149 (MQ=255)
                                        tCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGacac                                                                                                        >  1:7182523/1‑149 (MQ=255)
                                          gAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACtt                                                                                                      >  2:2453058/1‑149 (MQ=255)
                                           aaCCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGTGAAAACGGACAACAGACGATTCATGACACTTa                                                                                                     <  1:3686802/149‑1 (MQ=255)
                                            aCCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTAc                                                                                                    <  2:4908400/149‑1 (MQ=255)
                                               gttgttGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTc                                                                                                 >  2:7279486/1‑149 (MQ=255)
                                                   ttgGCCGCTGCCGGCAACGGGGTTCGCGGCAACACCGGCGCTTAGTACGGGTCGTTTGGCTTGGGCGTCGGTGAGACGCTACCTGCCAGCAACACCG‑CCCCACCAGGGAGAAAACGTACAACAGAAGATTAATGACACTTACCTCACgt                                                                                             <  2:415216/149‑2 (MQ=255)
                                                     ggCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGCCGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGgc                                                                                           <  2:1756563/149‑1 (MQ=255)
                                                       ccGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGgcgc                                                                                         <  2:4670511/149‑1 (MQ=255)
                                                       ccGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGgcgc                                                                                         >  2:5207733/1‑149 (MQ=255)
                                                       ccGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTAGTTGGGCTTGGGCGTCGGTGCGACGAAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGAAGATTCATGACACTTACATCACGGgcgc                                                                                         >  1:6656465/1‑149 (MQ=255)
                                                        cGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCa                                                                                        <  2:1845133/149‑1 (MQ=255)
                                                          cTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGc                                                                                      <  1:3058022/149‑1 (MQ=255)
                                                              cGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAg                                                                                  <  2:559185/149‑1 (MQ=255)
                                                               ggCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGt                                                                                 <  1:4079007/149‑1 (MQ=255)
                                                               ggCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGt                                                                                 >  2:4957702/1‑149 (MQ=255)
                                                               ggCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGt                                                                                 >  2:5302614/1‑149 (MQ=255)
                                                                gCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTg                                                                                <  1:7992904/149‑1 (MQ=255)
                                                                gCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTg                                                                                >  1:3815525/1‑149 (MQ=255)
                                                                  aaCGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGAc                                                                              <  1:4435265/149‑1 (MQ=255)
                                                                  aaCGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGAc                                                                              >  1:279542/1‑149 (MQ=255)
                                                                     ggggTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAgg                                                                           <  1:5096876/149‑1 (MQ=255)
                                                                         tGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCtt                                                                       >  2:3766116/1‑149 (MQ=255)
                                                                            gcgGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTaga                                                                    <  1:5643091/149‑1 (MQ=255)
                                                                              ggCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGc                                                                  <  1:96409/149‑1 (MQ=255)
                                                                               gCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCt                                                                 >  1:4527988/1‑149 (MQ=255)
                                                                                cAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTg                                                                <  1:7077326/149‑1 (MQ=255)
                                                                                   cacCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTg                                                             <  1:173032/149‑1 (MQ=255)
                                                                                     ccGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAg                                                           >  2:5987147/1‑149 (MQ=255)
                                                                                        gcgcATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACACACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTaa                                                        <  2:6913990/149‑1 (MQ=255)
                                                                                           cATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTaacga                                                     <  1:4199396/149‑1 (MQ=255)
                                                                                           cATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTaacga                                                     <  2:2821714/149‑1 (MQ=255)
                                                                                            aTAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTaacgaa                                                    <  2:6782323/149‑1 (MQ=255)
                                                                                               gTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAacgaacga                                                 <  1:4913097/149‑1 (MQ=255)
                                                                                               gTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAacgaacga                                                 >  1:3561807/1‑149 (MQ=255)
                                                                                                tACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGAc                                                <  1:4734489/149‑1 (MQ=255)
                                                                                                tACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGAc                                                <  2:6066237/149‑1 (MQ=255)
                                                                                                      tCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATc                                          >  1:5288562/1‑149 (MQ=255)
                                                                                                        gTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTg                                        <  2:6234612/149‑1 (MQ=255)
                                                                                                         ttgggcttgggcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTgg                                       <  1:6295751/149‑1 (MQ=255)
                                                                                                          tgggcttgggcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGt                                      <  1:3372294/149‑1 (MQ=255)
                                                                                                          tgggcttgggcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGt                                      >  1:2988684/1‑149 (MQ=255)
                                                                                                            ggcttgggcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCg                                    <  2:7828047/149‑1 (MQ=255)
                                                                                                             gcttgggcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCgg                                   <  2:7736773/149‑1 (MQ=255)
                                                                                                             gcttgggcGTCGGTGCGACGCAACATGCCAGCAACACCG‑CCCCCCCTGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGGTCAGCTGAGTGACTGGGCTTAGAGGTGTTTAGTAACGAACGACAGCATCTGGTCgg                                   <  2:4308598/149‑1 (MQ=255)
                                                                                                              cttgggcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACCGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGc                                  <  1:5481514/149‑1 (MQ=255)
                                                                                                              cttgggcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGc                                  <  2:2683162/149‑1 (MQ=255)
                                                                                                                  ggcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTg                              >  2:6687936/1‑149 (MQ=255)
                                                                                                                   gcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTgg                             <  1:2257944/149‑1 (MQ=255)
                                                                                                                    cGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGa                            >  1:6761648/1‑149 (MQ=255)
                                                                                                                      tCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGaaa                          >  1:7595821/1‑149 (MQ=255)
                                                                                                                          tGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGAc                      >  1:4855466/1‑149 (MQ=255)
                                                                                                                            cGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACtt                    >  2:1980534/1‑149 (MQ=255)
                                                                                                                            cGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACtt                    >  2:6327765/1‑149 (MQ=255)
                                                                                                                             gACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACttt                   <  2:2062197/149‑1 (MQ=255)
                                                                                                                             gACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACttt                   <  2:2101307/149‑1 (MQ=255)
                                                                                                                             gACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACttt                   <  2:3450638/149‑1 (MQ=255)
                                                                                                                               cGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTgg                 <  1:4646345/149‑1 (MQ=255)
                                                                                                                               cGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTgg                 <  2:6570931/149‑1 (MQ=255)
                                                                                                                               cGCAACCGGCCAGCCCCACCG‑CCCCCCCAGGGCGAACACGGACACCAGACGATTCATGACACTTACCGCCCGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTgg                 <  2:8008984/149‑1 (MQ=255)
                                                                                                                                   agcTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACCCTTACCTCTCGGGCGCAGCTGAGTGACAGGGCTTAGAGCGGGTGCGTAACGAACGACAGCATCTGGTCGGCCGTGGCACTGGCGTTGGAGtt             >  1:4826793/3‑149 (MQ=255)
                                                                                                                                     cTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCa           <  1:2453580/149‑1 (MQ=255)
                                                                                                                                     cTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCa           >  1:3945741/1‑149 (MQ=255)
                                                                                                                                        ccAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCAtct        <  1:4292556/149‑1 (MQ=255)
                                                                                                                                         cAGCAACACCGCCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCAtcg        >  2:7639482/1‑148 (MQ=255)
                                                                                                                                         cAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCAtctc       >  1:5974284/1‑149 (MQ=255)
                                                                                                                                          aGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCATCTCg      <  1:5207733/149‑1 (MQ=255)
                                                                                                                                             aaCACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCATCTCGTAg   >  1:7323882/1‑149 (MQ=255)
                                                                                                                                             aaCACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCATCTCGTAg   >  2:1561862/1‑149 (MQ=255)
                                                                                                                                              acacCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCATCTCGTAgg  >  2:5482854/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CGGTCGCTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGCCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCATCTCGTAGG  >  NC_002947/4983409‑4983700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑