Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,163,121 C→T A100V (GCG→GTG)  gtsA → mannose/glucose ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,163,1210CT99.5% 1442.6 / NA 385A100V (GCG→GTG) gtsAmannose/glucose ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (174/209);  minor base G (0/2);  total (174/211)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 388 total aligned reads displayed.

GGTCGAAAAAGACGGCTTCATCTGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGCGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCGGTGAATATCCACCGCATCAAC  >  NC_002947/1162975‑1163266
                                                                                                                                                  |                                                                                                                                                 
  tCGAAAAAGACGGCTTCATCTGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGcc                                                                                                                                               >  1:1603880/1‑149 (MQ=255)
   cGAAAAAGACGGCTTCATCTGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCa                                                                                                                                              <  1:6089163/149‑1 (MQ=255)
     aaaaaGACGGCTTCATCTGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCAcc                                                                                                                                            >  2:1371802/1‑149 (MQ=255)
     aaaaaGACGGCTTCATCTGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCAcc                                                                                                                                            >  1:1973333/1‑149 (MQ=255)
     aaaaaGACGGCTTCATCTGGAAGGACGGCGCCGTCGCCGGAGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGTCCGGCAACCCGCAGGGCGTCGCGCAGATCAAAGGACCGGACATCCAGGACTGGGTGGCCAcc                                                                                                                                            >  1:3422925/1‑149 (MQ=255)
       aaaGACGGCTTCATCTGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCgg                                                                                                                                          <  2:5352774/149‑1 (MQ=255)
       aaaGACGGCTTCATCTGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCgg                                                                                                                                          <  1:5722023/149‑1 (MQ=255)
            cGGCTTCATCTGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGc                                                                                                                                     >  2:1247316/1‑149 (MQ=255)
                ttCATCTGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGa                                                                                                                                 <  2:976569/149‑1 (MQ=255)
                      tGGCAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGa                                                                                                                           <  2:3899752/149‑1 (MQ=255)
                      tGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGa                                                                                                                           >  2:3043905/1‑149 (MQ=255)
                      tGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGa                                                                                                                           <  2:1459656/149‑1 (MQ=255)
                      tGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGa                                                                                                                           <  1:3696145/149‑1 (MQ=255)
                      tGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGa                                                                                                                           >  1:6378060/1‑149 (MQ=255)
                       ggAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGAt                                                                                                                          >  1:4272298/1‑149 (MQ=255)
                         aaGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGAtgt                                                                                                                        >  2:6098144/1‑149 (MQ=255)
                         aaGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGAtgt                                                                                                                        >  2:7981500/1‑149 (MQ=255)
                         aaGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGAtgt                                                                                                                        <  1:6625946/149‑1 (MQ=255)
                         aaGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGAtgt                                                                                                                        >  1:1081006/1‑149 (MQ=255)
                            gACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCt                                                                                                                     >  1:2386968/1‑149 (MQ=255)
                             aCGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTc                                                                                                                    >  2:6293866/1‑149 (MQ=255)
                              cGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCa                                                                                                                   <  1:2124807/149‑1 (MQ=255)
                               ggCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCaa                                                                                                                  <  2:3602099/149‑1 (MQ=255)
                                gcgcCGTCGCCTGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAg                                                                                                                 <  2:4614192/149‑1 (MQ=255)
                                gcgcCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAg                                                                                                                 >  2:4989994/1‑149 (MQ=255)
                                gcgcCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAg                                                                                                                 <  1:5825790/149‑1 (MQ=255)
                                 cgcCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAgg                                                                                                                <  1:732223/149‑1 (MQ=255)
                                 cgcCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAgg                                                                                                                <  1:4734345/149‑1 (MQ=255)
                                    cGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGAtg                                                                                                             <  1:7633827/149‑1 (MQ=255)
                                    cGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGAtg                                                                                                             >  1:1007223/1‑149 (MQ=255)
                                     gTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGAtgt                                                                                                            <  1:851226/149‑1 (MQ=255)
                                     gTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGAtgt                                                                                                            <  2:668383/149‑1 (MQ=255)
                                       cGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTgg                                                                                                          <  2:3525668/149‑1 (MQ=255)
                                          cggcggcggTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCa                                                                                                       <  1:6901101/149‑1 (MQ=255)
                                              ggcggTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCaagga                                                                                                   <  2:543171/149‑1 (MQ=255)
                                                 ggtggtGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCaaggaagg                                                                                                <  1:6555448/149‑1 (MQ=255)
                                                    ggtgCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGaaa                                                                                             >  2:7525106/1‑149 (MQ=255)
                                                    ggtgCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGaaa                                                                                             <  2:6652136/149‑1 (MQ=255)
                                                      tgCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGt                                                                                           <  2:6233796/149‑1 (MQ=255)
                                                       gCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTg                                                                                          <  1:7780824/149‑1 (MQ=255)
                                                        cAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTgg                                                                                         >  2:7411924/1‑149 (MQ=255)
                                                        cAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTgg                                                                                         >  2:1557885/1‑149 (MQ=255)
                                                         aaCGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTggg                                                                                        >  1:2859840/1‑149 (MQ=255)
                                                         aaCGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTggg                                                                                        <  2:67127/149‑1 (MQ=255)
                                                         aaCGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTggg                                                                                        >  1:1672824/1‑149 (MQ=255)
                                                           cGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGAc                                                                                      >  2:4291213/1‑149 (MQ=255)
                                                               cATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGAGGTGGCCAAGGAAGGAAAGTGGGACTCgc                                                                                  >  1:3582687/1‑149 (MQ=255)
                                                                aTGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCgct                                                                                 >  1:6187734/1‑149 (MQ=255)
                                                                aTGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCgct                                                                                 >  1:2983603/1‑149 (MQ=255)
                                                                aTGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACGGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCGAGGGAGGACAGTGGGCCTCgct                                                                                 >  2:3688400/1‑149 (MQ=255)
                                                                  gACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCgctgc                                                                               <  2:5958938/149‑1 (MQ=255)
                                                                      gTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGa                                                                           >  2:7664018/1‑149 (MQ=255)
                                                                        gCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACa                                                                         <  2:4479115/149‑1 (MQ=255)
                                                                         cTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACaa                                                                        >  2:1690551/1‑149 (MQ=255)
                                                                          tCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAg                                                                       <  1:702260/149‑1 (MQ=255)
                                                                           cAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGa                                                                      >  1:5048661/1‑149 (MQ=255)
                                                                             aaaGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGaaa                                                                    >  1:1199415/1‑149 (MQ=255)
                                                                             aaaGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGaaa                                                                    >  1:4345581/1‑149 (MQ=255)
                                                                             aaaGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGaaa                                                                    >  2:5497784/1‑149 (MQ=255)
                                                                              aaGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAg                                                                   >  2:4124642/1‑149 (MQ=255)
                                                                                gCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTg                                                                 <  2:231018/149‑1 (MQ=255)
                                                                                 ccGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTgg                                                                <  2:3841017/149‑1 (MQ=255)
                                                                                    cgcAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCg                                                             <  1:2644726/149‑1 (MQ=255)
                                                                                     gcAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACACGAAAGTGGCCGa                                                            >  2:5698223/1‑149 (MQ=255)
                                                                                      cAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGac                                                           >  2:6087901/1‑149 (MQ=255)
                                                                                        gTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGacac                                                         >  2:5113910/1‑149 (MQ=255)
                                                                                           gCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGt                                                      <  2:7147796/149‑1 (MQ=255)
                                                                                           gCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGt                                                      <  2:1846798/149‑1 (MQ=255)
                                                                                             cGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGa                                                    <  1:5640810/149‑1 (MQ=255)
                                                                                              ggCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGaa                                                   <  1:4194861/149‑1 (MQ=255)
                                                                                                 aaCCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTa                                                <  2:5923498/149‑1 (MQ=255)
                                                                                                 aaCCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTa                                                >  1:6297880/1‑149 (MQ=255)
                                                                                                    ccgccgGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTacga                                             <  1:3837769/149‑1 (MQ=255)
                                                                                                     cgccgGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTacgac                                            >  2:2945334/1‑149 (MQ=255)
                                                                                                     cgccgGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTacgac                                            <  1:4655617/149‑1 (MQ=255)
                                                                                                       ccgGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACgg                                          <  2:5586364/149‑1 (MQ=255)
                                                                                                            cGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACt                                     >  1:3392188/1‑149 (MQ=255)
                                                                                                                gcgcAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGt                                 <  2:707216/149‑1 (MQ=255)
                                                                                                                 cgcAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGtt                                >  1:1306874/1‑149 (MQ=255)
                                                                                                                  gcAGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTg                               >  1:6033521/1‑149 (MQ=255)
                                                                                                                   cAGCTCAAACGCCCGGACATCCAGGCCCGGGTGGCCACCGGCCGGCTCGACGCCGAGGTGCTCAAGGATGTCGCCAAGGAAGGAAAGTGGGACTCGCTTCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGc                              <  2:7279970/149‑1 (MQ=255)
                                                                                                                    aGATCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGcc                             <  2:2554794/149‑1 (MQ=255)
                                                                                                                       tCAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCCAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTa                          <  2:6504457/149‑1 (MQ=255)
                                                                                                                        cAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTAc                         >  1:6413408/1‑149 (MQ=255)
                                                                                                                        cAAAGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTAc                         >  1:392642/1‑149 (MQ=255)
                                                                                                                           aGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGGTGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCgg                      <  2:825730/149‑1 (MQ=255)
                                                                                                                           aGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCgg                      <  1:5142449/149‑1 (MQ=255)
                                                                                                                           aGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCgg                      >  2:2682651/1‑149 (MQ=255)
                                                                                                                           aGGCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCgg                      <  1:4507387/149‑1 (MQ=255)
                                                                                                                             gCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCGGTg                    >  1:3110782/1‑149 (MQ=255)
                                                                                                                             gCCCGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCGGTg                    <  2:3225650/149‑1 (MQ=255)
                                                                                                                              cccGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCGGTGa                   >  1:4849197/1‑149 (MQ=255)
                                                                                                                               ccGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCGGTGaa                  >  1:7589032/1‑149 (MQ=255)
                                                                                                                                cGGACATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCGGTGAat                 <  2:6850506/149‑1 (MQ=255)
                                                                                                                                   aCATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCGGTGAATATc              <  2:2127381/149‑1 (MQ=255)
                                                                                                                                   aCATCCAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCGGTGAATATc              <  2:3375743/149‑1 (MQ=255)
                                                                                                                                       ccAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCGGTGAATATCCAcc          >  1:2540149/1‑149 (MQ=255)
                                                                                                                                        cAGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAACCGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTTCGACGGTGACTACGTTGCCGTACCGGTGAATATCCACCg         <  1:805417/149‑1 (MQ=255)
                                                                                                                                         aGGACTGGGTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCGGTGAATATCCACCGc        >  2:5770327/1‑149 (MQ=255)
                                                                                                                                               gggTGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCGGTGAATATCCACCGCATCAAc  >  1:7204134/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGTCGAAAAAGACGGCTTCATCTGGAAGGACGGCGCCGTCGCCGGCGGCGGTGGTGCAACGGCCATGACCGTGCTCAAAAGCCGCGCAGTGGCCGGCAACCCGCCGGGCGTCGCGCAGATCAAAGGCCCGGACATCCAGGACTGGGCGGCCACCGGCCTGCTCGACGCCGATGTGCTCAAGGATGTGGCCAAGGAAGGAAAGTGGGACTCGCTGCTCGACAAGAAAGTGGCCGACACCGTGAAGTACGACGGTGACTACGTTGCCGTACCGGTGAATATCCACCGCATCAAC  >  NC_002947/1162975‑1163266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑