Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,163,732 A→G N304D (AAC→GAC)  gtsA → mannose/glucose ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,163,7320AG100.0% 1463.1 / NA 396N304D (AAC→GAC) gtsAmannose/glucose ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (186/210);  total (186/210)

Only 100 of 400 total aligned reads displayed.

TGGAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCT  >  NC_002947/1163586‑1163877
                                                                                                                                                  |                                                                                                                                                 
tGGAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTacgac                                                                                                                                                 >  2:1616398/1‑149 (MQ=255)
    aGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCg                                                                                                                                             <  2:6429578/149‑1 (MQ=255)
       cgccAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACt                                                                                                                                          <  2:1124359/149‑1 (MQ=255)
          cAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGt                                                                                                                                       >  1:2343977/1‑149 (MQ=255)
           aaGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGtt                                                                                                                                      <  2:2348496/149‑1 (MQ=255)
           aaGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGtt                                                                                                                                      <  1:2792587/149‑1 (MQ=255)
           aaGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTAACAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGtt                                                                                                                                      >  1:4134808/1‑149 (MQ=255)
                cATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTtggtgg                                                                                                                                 >  1:5963286/1‑149 (MQ=255)
                cATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTtggtgg                                                                                                                                 <  2:5320848/149‑1 (MQ=255)
                 aTTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGACAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTggtggt                                                                                                                                <  1:682606/149‑1 (MQ=255)
                 aTTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTggtggt                                                                                                                                <  2:1953596/149‑1 (MQ=255)
                  ttAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGgtggtg                                                                                                                               <  2:2479209/149‑1 (MQ=255)
                   tAACGGCAAGGCCGGTATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGgtgt                                                                                                                              >  1:5800710/1‑149 (MQ=255)
                     aCGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTc                                                                                                                            >  1:5227058/1‑149 (MQ=255)
                      cGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCa                                                                                                                           <  1:6373533/149‑1 (MQ=255)
                       ggcccggCCGGCATGCAGATCATGGGCGACTGGGCCACGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCaa                                                                                                                          <  2:7678163/144‑1 (MQ=255)
                       ggCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCaa                                                                                                                          <  2:4124352/149‑1 (MQ=255)
                        gCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAg                                                                                                                         <  1:1376512/149‑1 (MQ=255)
                        gCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAg                                                                                                                         <  2:4227476/149‑1 (MQ=255)
                         cAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGc                                                                                                                        >  1:4189848/1‑149 (MQ=255)
                         cAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGc                                                                                                                        <  2:321736/149‑1 (MQ=255)
                                ggCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGaacaa                                                                                                                 <  2:5862014/149‑1 (MQ=255)
                                ggCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGaacaa                                                                                                                 <  1:1282160/149‑1 (MQ=255)
                                ggCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGaacaa                                                                                                                 >  1:4924803/1‑149 (MQ=255)
                                   aTGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGc                                                                                                              <  1:361358/149‑1 (MQ=255)
                                   aTGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGc                                                                                                              >  1:5442629/1‑149 (MQ=255)
                                     gCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTg                                                                                                            <  1:2951881/149‑1 (MQ=255)
                                      cAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTgg                                                                                                           <  1:1993419/149‑1 (MQ=255)
                                        gATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCa                                                                                                         >  2:3912108/1‑149 (MQ=255)
                                        gATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCa                                                                                                         >  1:2544449/1‑149 (MQ=255)
                                                 cGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACCTCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgctg                                                                                                >  1:7178904/1‑149 (MQ=255)
                                                 cGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgctg                                                                                                >  1:7049810/1‑149 (MQ=255)
                                                 cGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgctg                                                                                                >  2:6813397/1‑149 (MQ=255)
                                                 cGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgctg                                                                                                >  2:719084/1‑149 (MQ=255)
                                                 cGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgctg                                                                                                >  1:5887697/1‑149 (MQ=255)
                                                      gggCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcag                                                                                           >  1:4583974/1‑149 (MQ=255)
                                                       ggCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcagc                                                                                          <  1:7866876/149‑1 (MQ=255)
                                                        gCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcagca                                                                                         >  1:7750252/1‑149 (MQ=255)
                                                           aaGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGa                                                                                      <  1:6571269/149‑1 (MQ=255)
                                                              agCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACAt                                                                                   <  1:6994643/149‑1 (MQ=255)
                                                                 gAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGc                                                                                <  2:4905558/149‑1 (MQ=255)
                                                                   aTGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGccc                                                                              <  1:3662942/149‑1 (MQ=255)
                                                                       aCCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCaa                                                                          <  1:1812086/149‑1 (MQ=255)
                                                                          cTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGgt                                                                       >  2:6268590/1‑149 (MQ=255)
                                                                           tGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGgtg                                                                      >  1:6491854/1‑149 (MQ=255)
                                                                            ggCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGgtgt                                                                     >  2:3133557/1‑149 (MQ=255)
                                                                              cGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTg                                                                   <  2:789934/149‑1 (MQ=255)
                                                                              cGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTg                                                                   <  2:478203/149‑1 (MQ=255)
                                                                                aagaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTggg                                                                 <  1:7325377/149‑1 (MQ=255)
                                                                                  gaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTg                                                               <  1:2201983/149‑1 (MQ=255)
                                                                                  gaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTg                                                               <  1:3966009/149‑1 (MQ=255)
                                                                                   aaaaCTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAGCAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGa                                                              <  2:5457409/149‑1 (MQ=255)
                                                                                   aaaaCTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGa                                                              <  1:5566349/149‑1 (MQ=255)
                                                                                    aaaCTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAg                                                             <  1:626026/149‑1 (MQ=255)
                                                                                    aaaCTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAg                                                             >  2:1722945/1‑149 (MQ=255)
                                                                                      aCTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGa                                                           >  1:3737962/1‑149 (MQ=255)
                                                                                         gctgGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACtt                                                        <  1:958156/149‑1 (MQ=255)
                                                                                         gctgGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACtt                                                        <  2:4332429/149‑1 (MQ=255)
                                                                                         gctgGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACtt                                                        <  1:209820/149‑1 (MQ=255)
                                                                                          ctgGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTc                                                       <  2:2195852/149‑1 (MQ=255)
                                                                                             gCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAg                                                    >  1:1013840/1‑149 (MQ=255)
                                                                                             gCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAg                                                    <  2:5681863/149‑1 (MQ=255)
                                                                                              cAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGa                                                   <  2:3350371/149‑1 (MQ=255)
                                                                                              cAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGa                                                   >  1:4714843/1‑149 (MQ=255)
                                                                                              cAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGa                                                   <  2:2704131/149‑1 (MQ=255)
                                                                                               aaGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGaa                                                  <  2:7453980/149‑1 (MQ=255)
                                                                                                aGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAg                                                 >  2:2576285/1‑149 (MQ=255)
                                                                                                aGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAg                                                 >  2:6604621/1‑149 (MQ=255)
                                                                                                aGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAg                                                 <  1:1616398/149‑1 (MQ=255)
                                                                                                   aTTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTc                                              >  1:886878/1‑149 (MQ=255)
                                                                                                   aTTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACACGTTGGTGGAGTTCAAGCAGAACAACGCTGGCACCTCAGCAGGTCAGCAGGACATCGACCGCAAGGTGATTGGTGAGGACTTCCAGAAGGTc                                              >  2:4620541/1‑149 (MQ=255)
                                                                                                    ttACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCt                                             <  2:6086003/149‑1 (MQ=255)
                                                                                                         aGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGc                                        >  1:7385902/1‑149 (MQ=255)
                                                                                                         aGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGc                                        >  1:3460985/1‑149 (MQ=255)
                                                                                                          gtgcgtgcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCa                                       <  1:515403/149‑1 (MQ=255)
                                                                                                              gtgcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATcaa                                   <  1:6674133/149‑1 (MQ=255)
                                                                                                                 ccGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATcaacaa                                >  2:6692044/1‑149 (MQ=255)
                                                                                                                  cGTTCCCCGGTACTGACTAGTCCTTCCTGTACGACATATACTCGTTGGTGGTGTTCAAGCAGAACAACTCTGGCACCTCTGCTGGTCAGCAGTACTTCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCATCATCAACAAg                               <  2:5267442/149‑1 (MQ=255)
                                                                                                                   gTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAgg                              >  1:4043328/1‑149 (MQ=255)
                                                                                                                     tCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGt                            <  1:6188693/149‑1 (MQ=255)
                                                                                                                     tCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGt                            <  2:2297564/149‑1 (MQ=255)
                                                                                                                      ccccGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGtt                           <  1:4353189/149‑1 (MQ=255)
                                                                                                                        ccGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCg                         <  2:1462151/149‑1 (MQ=255)
                                                                                                                         cGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGa                        >  2:5636342/1‑149 (MQ=255)
                                                                                                                         cGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGa                        >  1:5681963/1‑149 (MQ=255)
                                                                                                                         cGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGa                        <  2:7044885/149‑1 (MQ=255)
                                                                                                                            tACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATcc                     <  1:3184475/149‑1 (MQ=255)
                                                                                                                            tACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATcc                     >  2:2920502/1‑149 (MQ=255)
                                                                                                                            tACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATcc                     >  1:6121690/1‑149 (MQ=255)
                                                                                                                               tGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCgg                  <  2:7789144/149‑1 (MQ=255)
                                                                                                                                gACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGt                 >  2:4479045/1‑149 (MQ=255)
                                                                                                                                  cAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTAc               >  2:2103388/1‑149 (MQ=255)
                                                                                                                                    aGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGc             >  1:112978/1‑149 (MQ=255)
                                                                                                                                    aGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGc             >  1:5639828/1‑149 (MQ=255)
                                                                                                                                    aGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGc             >  1:1514557/1‑149 (MQ=255)
                                                                                                                                      tccttcctGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCaa           <  1:7564079/149‑1 (MQ=255)
                                                                                                                                         ttcctGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGa        <  2:3579918/149‑1 (MQ=255)
                                                                                                                                           cctGTACGACGTCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATACCGGTACGCAACGACa      <  2:7303025/149‑1 (MQ=255)
                                                                                                                                             tGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATg    >  2:3041136/1‑149 (MQ=255)
                                                                                                                                             tGTACGACATAGACTCGTTGGTGGTGTTCAAGCAGAACAACGATGGCACCTCTGCTGGTCAGCAGGACATAGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATg    >  2:3667274/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGGAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCT  >  NC_002947/1163586‑1163877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑