BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,602,747	T→A	5.2%	Q686L (CAG→CTG)	PP_4071 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,602,747	0	T	A	5.2%	859.3 / 22.8	244	Q686L (CAG→CTG)	PP_4071	membrane protein
Reads supporting (aligned to +/- strand): ref base T (107/124); new base A (4/8); total (112/132)
Fisher's exact test for biased strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 264 total aligned reads displayed.

CCTTGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCAGCTGC  >  NC_002947/4602598‑4602896

 cTTGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCctgctg                                                                                                                                                      >  1:4096213/1‑150 (MQ=255)

 cTTGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTAGGCctgctg                                                                                                                                                      >  2:1527457/1‑150 (MQ=255)

  ttGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCtgctgc                                                                                                                                                     >  1:5645719/1‑150 (MQ=255)

  ttGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCtgctgc                                                                                                                                                     >  1:3447994/1‑150 (MQ=255)

  ttGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCtgctgc                                                                                                                                                     >  1:780881/1‑150 (MQ=255)

          tttGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGcc                                                                                                                                             >  1:1560389/1‑150 (MQ=255)

          tttGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGcc                                                                                                                                             >  2:5732036/1‑150 (MQ=255)

           ttGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGccc                                                                                                                                            <  1:168150/150‑1 (MQ=255)

           ttGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGAAGCAGCAGGccc                                                                                                                                            <  1:4736848/150‑1 (MQ=255)

               cAGTGAGGATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGAGAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGAGCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCAt                                                                                                                                        >  2:5941134/1‑150 (MQ=255)

               cAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCAt                                                                                                                                        >  2:2510508/1‑150 (MQ=255)

               cAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGAAGCAGCAGGcccagga                                                                                                                                        <  1:6211445/150‑5 (MQ=255)

               cAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGAAGCAGCAGGcccagga                                                                                                                                        <  1:921458/150‑5 (MQ=255)

                aGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATa                                                                                                                                       <  2:3821112/150‑1 (MQ=255)

                     gCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACAcgc                                                                                                                                  <  1:7405727/150‑1 (MQ=255)

                     gCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACAcgc                                                                                                                                  <  2:7291869/150‑1 (MQ=255)

                     gCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACAcgc                                                                                                                                  <  1:2953412/150‑1 (MQ=255)

                     gCATTGGCGCCAGCAAAACACCATGGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAACCGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACAcgc                                                                                                                                  <  2:6805986/150‑1 (MQ=255)

                       aTTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGt                                                                                                                                <  1:248639/150‑1 (MQ=255)

                          ggCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgt                                                                                                                             <  1:6703175/150‑1 (MQ=255)

                          ggCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgt                                                                                                                             <  1:3651708/150‑1 (MQ=255)

                                aGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCtt                                                                                                                                                   >  1:433477/1‑122 (MQ=255)

                                aGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCtt                                                                                                                                                   <  2:433477/122‑1 (MQ=255)

                                aGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAAc                                                                                                                       >  2:1842193/1‑150 (MQ=255)

                                 gCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACg                                                                                                                      <  2:1049201/150‑1 (MQ=255)

                                  cAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACgg                                                                                                                     <  2:2933590/150‑1 (MQ=255)

                                   aaaaCACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGc                                                                                                                    >  1:1365023/1‑150 (MQ=255)

                                     aaCACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGAAGCAGCAGGccca                                                                                                                                           >  1:5074656/1‑124 (MQ=255)

                                     aaCACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGAAGCAGCAGGccca                                                                                                                                           <  2:5074656/125‑2 (MQ=255)

                                       cacCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCt                                                                                                                >  1:1425134/1‑150 (MQ=255)

                                        acCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCtt                                                                                                               >  2:3732662/1‑150 (MQ=255)

                                               tCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAAt                                                                                                        <  1:6569862/150‑1 (MQ=255)

                                                    cTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTctg                                                                                                   <  1:6944371/150‑1 (MQ=255)

                                                     tGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTctgc                                                                                                  <  1:360886/150‑1 (MQ=255)

                                                      ggCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTctgct                                                                                                 <  2:5670530/150‑1 (MQ=255)

                                                         tCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCa                                                                                              >  1:4742750/1‑150 (MQ=255)

                                                         tCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCa                                                                                              <  1:6073451/150‑1 (MQ=255)

                                                         tCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCa                                                                                              <  1:2323123/150‑1 (MQ=255)

                                                            aGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGt                                                                                           <  1:5899942/150‑1 (MQ=255)

                                                                 gAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTg                                                                                      >  2:5569772/1‑150 (MQ=255)

                                                                         cTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTg                                                                              >  2:4635875/1‑150 (MQ=255)

                                                                          tGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTgg                                                                             >  1:4894173/1‑150 (MQ=255)

                                                                          tGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTgg                                                                             <  2:3787316/150‑1 (MQ=255)

                                                                          tGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTgg                                                                             <  1:4310203/150‑1 (MQ=255)

                                                                           ggTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGc                                                                            <  1:6678734/150‑1 (MQ=255)

                                                                            gTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCg                                                                           >  1:2742145/1‑150 (MQ=255)

                                                                             tAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGa                                                                          <  2:1425134/150‑1 (MQ=255)

                                                                             tAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGa                                                                          <  1:3042447/150‑1 (MQ=255)

                                                                             tAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGa                                                                          >  1:2175972/1‑150 (MQ=255)

                                                                                aCGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATAc                                                                       >  1:3193377/1‑150 (MQ=255)

                                                                                aCGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATAc                                                                       <  1:1842193/150‑1 (MQ=255)

                                                                                 cGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACt                                                                      >  2:1367477/1‑150 (MQ=255)

                                                                                    cATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCg                                                                   <  1:2366407/150‑1 (MQ=255)

                                                                                     aTCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCgg                                                                  >  2:2704917/1‑150 (MQ=255)

                                                                                      tCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGc                                                                 >  1:5460333/1‑150 (MQ=255)

                                                                                        gAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAg                                                               <  2:6350302/150‑1 (MQ=255)

                                                                                            gCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCg                                                           <  1:5569772/150‑1 (MQ=255)

                                                                                            gCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCg                                                           <  2:4878518/150‑1 (MQ=255)

                                                                                            gCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCg                                                           <  2:5621078/150‑1 (MQ=255)

                                                                                            gCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCg                                                           <  2:4077073/150‑1 (MQ=255)

                                                                                            gCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCg                                                           <  2:3546743/150‑1 (MQ=255)

                                                                                              aGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGt                                                         >  1:3147165/1‑150 (MQ=255)

                                                                                                gCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTAt                                                       >  2:3356717/1‑150 (MQ=255)

                                                                                                  cccACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATcc                                                     <  1:5519445/150‑1 (MQ=255)

                                                                                                  cccACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATcc                                                     <  2:236162/150‑1 (MQ=255)

                                                                                                  cccACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATcc                                                     >  1:2061652/1‑150 (MQ=255)

                                                                                                     aCCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCcgcg                                                  <  1:3956100/150‑1 (MQ=255)

                                                                                                       cTGATGACGCAGGTCCAGCCCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATCCACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGAGACTGCGGCAGGTCGGTATCCGCGTa                                                <  2:6551924/150‑1 (MQ=255)

                                                                                                            gaCGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCa                                           <  1:1057058/150‑1 (MQ=255)

                                                                                                              cGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCATTGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAgc                                         <  1:1011026/150‑1 (MQ=255)

                                                                                                                 aGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGtt                                                                                                                               <  2:4449800/61‑1 (MQ=255)

                                                                                                                 aGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGtt                                                                                                                               >  1:4449800/1‑61 (MQ=255)

                                                                                                                   gTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATa                                    <  2:6139955/150‑1 (MQ=255)

                                                                                                                   gTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATa                                    <  1:6446912/150‑1 (MQ=255)

                                                                                                                     ccAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACt                                  >  1:4174184/1‑150 (MQ=255)

                                                                                                                       aGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTc                                >  1:3333487/1‑150 (MQ=255)

                                                                                                                       aGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTc                                <  2:3918201/150‑1 (MQ=255)

                                                                                                                        gACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCa                               <  2:257529/150‑1 (MQ=255)

                                                                                                                        gACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCa                               <  1:3372469/150‑1 (MQ=255)

                                                                                                                         aCCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAg                              >  1:7129949/1‑150 (MQ=255)

                                                                                                                           cGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGt                            >  1:1202294/1‑150 (MQ=255)

                                                                                                                              tATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGcc                         >  1:7289839/1‑150 (MQ=255)

                                                                                                                              tATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGcc                         <  1:4759368/150‑1 (MQ=255)

                                                                                                                               aTGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCg                        >  1:3840575/1‑150 (MQ=255)

                                                                                                                                  cAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGGTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCAt                     >  2:5523414/1‑150 (MQ=255)

                                                                                                                                   aaCTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATg                    >  2:7405360/1‑150 (MQ=255)

                                                                                                                                   aaCTGCTCCTGGGCCTGCCGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATg                    >  2:4290900/1‑150 (MQ=255)

                                                                                                                                    aCTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGa                   <  1:2243835/150‑1 (MQ=255)

                                                                                                                                      tGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGt                                                             >  2:2038371/1‑106 (MQ=255)

                                                                                                                                       gCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGt                <  1:5998893/150‑1 (MQ=255)

                                                                                                                                        cTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGtc               >  1:5175077/1‑150 (MQ=255)

                                                                                                                                         tCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCa                               <  1:583211/133‑1 (MQ=255)

                                                                                                                                             gggCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGtt          <  1:1493561/150‑1 (MQ=255)

                                                                                                                                             gggCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGtt          <  2:3078971/150‑1 (MQ=255)

                                                                                                                                               gCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGc        <  1:2704917/150‑1 (MQ=255)

                                                                                                                                                 ctgctgCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCAg      >  2:5178929/1‑150 (MQ=255)

                                                                                                                                                 ctgctgCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCAg      >  2:4183785/1‑150 (MQ=255)

                                                                                                                                                   gctgctTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCAGCt    <  1:107698/150‑1 (MQ=255)

                                                                                                                                                     tgctTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCAGCTGc  >  1:3897179/1‑150 (MQ=255)

                                                                                                                                                     tgctTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCAGCTGc  <  2:2299049/150‑1 (MQ=255)

CCTTGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCAGCTGC  >  NC_002947/4602598‑4602896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`