BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,102,219	G→C	8.9%	intergenic (+213/+79)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,102,219	0	G	C	8.9%	468.4 / 29.4	146	intergenic (+213/+79)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base G (75/58); new base C (7/6); total (82/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

Only 100 of 147 total aligned reads displayed.

TGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGG  >  NC_002947/5102071‑5102368

tGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGa                                                                                                                                                       <  2:899823/150‑1 (MQ=255)

tGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGa                                                                                                                                                       <  2:5180402/150‑1 (MQ=255)

tGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGa                                                                                                                                                       <  1:6473868/150‑1 (MQ=255)

      gATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAg                                                                                                                                                 <  1:3172543/150‑1 (MQ=255)

       aTGCACAAACTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAgg                                                                                                                                                <  1:1135684/150‑1 (MQ=255)

       aTGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAgg                                                                                                                                                <  1:4167764/150‑1 (MQ=255)

       aTGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAgg                                                                                                                                                <  2:6892510/150‑1 (MQ=255)

       aTGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAgg                                                                                                                                                <  2:3777048/150‑1 (MQ=255)

       aTGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAgg                                                                                                                                                <  1:1919326/150‑1 (MQ=255)

       aTGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAgg                                                                                                                                                <  2:6892714/150‑1 (MQ=255)

        tGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAggg                                                                                                                                               <  1:4857751/150‑1 (MQ=255)

           acaAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGAc                                                                                                                                            <  1:6278020/150‑1 (MQ=255)

           acaAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGAc                                                                                                                                            >  2:2665034/1‑150 (MQ=255)

            caAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAggctaag                                                                                                                                           <  1:6741207/150‑6 (MQ=255)

             aaaaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAg                                                                                                                                          <  1:7329799/150‑1 (MQ=255)

             aaaaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAg                                                                                                                                          <  1:151919/150‑1 (MQ=255)

             aaaaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGGCCGGGGACAg                                                                                                                                          >  1:2812725/1‑150 (MQ=255)

               aaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCt                                                                                                                                        <  2:6222683/150‑1 (MQ=255)

                 tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                     <  1:1541186/150‑1 (MQ=255)

                 tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                     <  1:6479602/150‑1 (MQ=255)

                 tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                     <  1:4680989/150‑1 (MQ=255)

                    tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTCCCGCGAAAGCGTGATGTGAGGGGACAGCT‑TAGcc                                                                                                                                  >  1:4607456/1‑150 (MQ=255)

                     gACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGTCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCt                                                                                                                                 <  2:2681362/150‑1 (MQ=255)

                      aCTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                                                                                                                <  2:1946024/150‑1 (MQ=255)

                        tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGaa                                                                                                                              >  2:1591392/1‑150 (MQ=255)

                        tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAgg                                                                                                                                                     <  1:3927959/128‑1 (MQ=255)

                        tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                              <  2:5112093/150‑1 (MQ=255)

                        tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                              <  2:5112114/150‑1 (MQ=255)

                          cGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCt‑t                                                                                                                                      <  1:3336250/140‑1 (MQ=255)

                          cGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCt‑t                                                                                                                                      >  2:3336250/1‑140 (MQ=255)

                          cGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACt                                                                                                                            >  2:5278961/1‑150 (MQ=255)

                          cGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTAAGGGGACAGCT‑TAGCCTGAACt                                                                                                                            >  1:2359182/1‑150 (MQ=255)

                           gATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCGGAAcgg                                                                                                                           >  2:3533623/1‑148 (MQ=255)

                             tGCACCGAAACGGGCCTGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                         <  1:379159/150‑1 (MQ=255)

                             tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                         <  1:3426581/150‑1 (MQ=255)

                                aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTGACGCa                                                                                                                      >  1:4184813/1‑150 (MQ=255)

                                 ccGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGGGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAt                                                                                                                     >  2:3501507/1‑150 (MQ=255)

                                     aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgc                                                                                                                 <  1:6129184/150‑1 (MQ=255)

                                      aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                <  1:811573/150‑1 (MQ=255)

                                       cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCTATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                               <  1:4217622/150‑1 (MQ=255)

                                       cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                               <  2:6234433/150‑1 (MQ=255)

                                       cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                               <  1:2244126/150‑1 (MQ=21)

                                       cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCGGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGACTGCGTGAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                               <  2:1666942/150‑1 (MQ=21)

                                           ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGTCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCaa                                                                                                           <  1:1464818/150‑1 (MQ=255)

                                           ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGcca                                                                                                           >  2:5653923/1‑148 (MQ=255)

                                            cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGAGGTCAGGGGCCAGCT‑TTGCCGGTCCGGACGCATTCGCGGGCacg                                                                                                          >  1:6745363/1‑148 (MQ=255)

                                                tttGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACTTTAACCGATGATTGTGGGTTAACAATGCAAACGGGGGCTTGCCCGCGATTGCGTGCGGTCAGGGGACAGCT‑TAGCCTTAACTGACGCATTCGCGGGCAAGCCCg                                                                                                      <  2:2579606/149‑1 (MQ=255)

                                                    tttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTCACAATGCAAAAGCGGGCGTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACGGCCGCCGTCGCGGGCCAGcccccgcc                                                                                                  >  2:3938585/1‑145 (MQ=255)

                                                     ttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGGCAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCGccg                                                                                                 >  1:3656023/1‑149 (MQ=255)

                                                        cTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCGTTCGCGGGc                                                                                                             >  1:575147/1‑135 (MQ=255)

                                                        cTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCGTTCGCGGGc                                                                                                             <  2:575147/135‑1 (MQ=255)

                                                         tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAg                                                                                             >  2:4894390/1‑150 (MQ=255)

                                                         tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCATGCCCCCGAATGCGTGAGGTAAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAg                                                                                             >  1:598923/1‑150 (MQ=255)

                                                          ggCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCTGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTTTTGCatt                                                                                            <  2:1193785/150‑3 (MQ=17)

                                                             gcCCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGCATGCGGGGGGTCAGGGGCCAGCG‑TAGCCGGCACGGACCCAATCGCGGGCCAGCCCCCGCCGGCAggggg                                                                                         >  1:2109142/1‑150 (MQ=255)

                                                             gcCCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCCCGAAAGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACTGACGCAATAGCCGGCAAGCCCCCGCCGGCAggggg                                                                                         >  1:2108788/1‑150 (MQ=255)

                                                             gcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAggggg                                                                                         >  2:3935647/1‑150 (MQ=255)

                                                               cccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGGCCCCGAATGCGTGAGGTCAGGGGGCAGCT‑TTGCCTGTACAGACGCCATAGCGGGGCAGCCCCCGCCAGCAgggggga                                                                                       >  1:4033123/1‑148 (MQ=255)

                                                                         tgCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCGCCTGCAGGGGGTAGTGCCgggcc                                                                             >  2:6582317/1‑148 (MQ=255)

                                                                                  ggTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTACCCCGAAATCGTGTGGTGAGGGGACAGCA‑TATCCTGTAAGGAAGCAATAGCGGGCAACCCCGCTCCTGCAGGGGGGGTTTGCGGGGAGGGTTTaag                                                                    >  1:4585621/1‑148 (MQ=255)

                                                                                   gTGACAGAACAGTAAGCGATGATTGTCGGGTAACAATGCAAATGCGGGCTTGGGCGCGTATGGGTGAGGTGGGGGGTCAGGT‑TTGCCTGTAGTGACGGATTGGGGGGCAAGGCCGCTGCTGGAGGGGGTTTTGGGGGGGAGGGttttagg                                                                   >  2:1041355/1‑146 (MQ=255)

                                                                                   gTGACAGAACAGTAAGCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTTCCCGCGAATGCGTGTGGTGAGGGGACAGGT‑TTGGGTGTAGTGTCGCATTGGCGGGCAAGCCCGCTCGTGGAGGGGGTTTTGGCGGGTAGGGTTTAAGc                                                                   >  2:1041336/1‑150 (MQ=255)

                                                                                      aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGACTGCGGGAGGTCCGGGGACAGCG‑TTGCCTGGACTGACGCAGTCGCGGGCCACCCCCCTCCGGCCCGGGGGATTGCCGGGCAGGGGTTACGCCgc                                                                >  1:4836354/1‑149 (MQ=255)

                                                                                        aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTGACGCATTCGCGGGCAAGCCCCCTCCTGCAGGGGGTATTTCCGGGCAGGGTTTACGCCGCAg                                                              >  2:1041332/1‑150 (MQ=255)

                                                                                               tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCCCCAAAGCCGGAGGTCAGGGGACCCCG‑TAGCCTGAACGGACCCAATCGCGGGCCACCCCCCTCCAGCAGGGGGTATTGCCGGGCAGGGGTTACGCCCAAGCCACGtt                                                       >  2:48115/1‑150 (MQ=255)

                                                                                                aaCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTCCCCCGCAAGCGTGAGGTCAGGGGACAGCA‑TAGCCGGAACAGACCCAATAGCGGGCAAGCCCGCACCGGCAGGGGGTATTTCCGGGAAGGGTTTAACCCGAACCCACGttg                                                      >  1:2883454/1‑149 (MQ=255)

                                                                                                aaCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTCCCCCGAATGCGTGAGGTGAGGGGCCAGCT‑TAGCCGGAACTGACGCAATCGCGGGCAAGCCCCCTCCGGCAGGGGGTATTTCCGGGAAGGGTTTACGCCGAAGCCAGGTTc                                                      >  1:4211498/1‑150 (MQ=255)

                                                                                                  ccGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCAAGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCACCAGCAGGGGGGATTGCCGGGAAGGGTTTACGCCGAAGCCAGGttaaa                                                    >  1:1732435/1‑147 (MQ=255)

                                                                                                    gatgatTGTCGGGTAACAATGCAAATGCGGGCTTGCCCGCGAATTGGTGTGGTGAGGGGTCAGGT‑TAGGGTGAAATGACGCATTCGCGGGCAAGCCCGCTGCTGGAGGGGGTATTGCCGGGTAGGGTTTACGCCGAAGCCAGGTTAAtgg                                                  >  2:837664/1‑148 (MQ=255)

                                                                                                    gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCCAAAGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAAGCATTCGCGGGCAAGCCCGCTCCAGCAGGGGGGATTGCCGGGAACGGTTTACGCCGAAGCCAGGTTCATCg                                                  >  2:3881478/1‑150 (MQ=255)

                                                                                                    gatgatTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAATGCGTGAGGTCAGGGGACCGCG‑TAGCCTGAACTGACCCATTCGCGGGCAAGCCCGCTCCAGCAGGGGGTATTGCCGGGACGGGTTTACGCCGAAGCCAGGTTCATCg                                                  >  1:5452051/1‑150 (MQ=255)

                                                                                                               ggTAACAATGCAAAAGCGGGCTTGCCCCCGCATGCGTGAGGTCAGGGGACAGCG‑TAGCCGGAACTGACGCATTCGCGGGCAAGCCCGCTCCGGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGGTCATCGCCGTGTgggga                                       >  2:3534686/1‑148 (MQ=255)

                                                                                                                       tGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCAGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTCGTGGGTATCGATCAg                               >  2:4269643/1‑150 (MQ=255)

                                                                                                                          attcgCGGGCAAGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                            >  1:6185254/5‑150 (MQ=255)

                                                                                                                          aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                            >  2:6943031/1‑150 (MQ=255)

                                                                                                                          aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                            >  2:3665459/1‑150 (MQ=255)

                                                                                                                          aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                            >  2:4596533/1‑150 (MQ=255)

                                                                                                                          aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACAGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGACAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                            >  2:2230659/1‑150 (MQ=255)

                                                                                                                            aaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCt                          >  2:5540351/1‑150 (MQ=255)

                                                                                                                            aaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCt                          >  2:3427650/1‑150 (MQ=255)

                                                                                                                                  gCTTGCCCGCTAATGCGTGAGGTCTGGGGACAGCT‑TCGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                    <  2:3656023/150‑1 (MQ=255)

                                                                                                                                  gCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                    >  2:4530282/1‑150 (MQ=255)

                                                                                                                                    ttGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAGTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcaca                  >  1:6767963/1‑150 (MQ=255)

                                                                                                                                        ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg              >  2:1172602/1‑150 (MQ=255)

                                                                                                                                        ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg              >  2:5362765/1‑150 (MQ=255)

                                                                                                                                        ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTTCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATc                                                   >  2:3422029/1‑113 (MQ=11)

                                                                                                                                        ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTTCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATc                                                   <  1:3422029/113‑1 (MQ=11)

                                                                                                                                        ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg              >  1:5944621/1‑150 (MQ=21)

                                                                                                                                        ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg              >  1:5476414/1‑150 (MQ=21)

                                                                                                                                        ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg              >  1:673600/1‑150 (MQ=21)

                                                                                                                                         cgcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg             >  2:468615/1‑150 (MQ=255)

                                                                                                                                          gctattGCGTGTGGTCTGGGGACAGCT‑TAGCCTGAACTGACGCTTTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg            <  2:5974859/145‑1 (MQ=255)

                                                                                                                                          gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg            >  2:6947237/1‑150 (MQ=255)

                                                                                                                                          gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg            >  2:3158651/1‑150 (MQ=255)

                                                                                                                                                  gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa    >  2:254331/1‑150 (MQ=255)

                                                                                                                                                  gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa    >  2:6599351/1‑150 (MQ=255)

                                                                                                                                                  gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa    >  1:3237932/1‑150 (MQ=255)

                                                                                                                                                  gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa    >  1:5146069/1‑150 (MQ=255)

                                                                                                                                                  gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa    >  1:5244367/1‑150 (MQ=255)

                                                                                                                                                    gAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAgg  >  1:114090/1‑150 (MQ=255)

TGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGG  >  NC_002947/5102071‑5102368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`