BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,102,228	G→C	8.9%	intergenic (+222/+70)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,102,228	0	G	C	8.9%	443.1 / 27.2	135	intergenic (+222/+70)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base G (80/43); new base C (7/5); total (87/48)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 142 total aligned reads displayed.

TGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATA  >  NC_002947/5102079‑5102376

tGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAggg                                                                                                                                                       <  1:4857751/150‑1 (MQ=255)

tGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAggg                                                                                                                                                       <  1:4857551/150‑1 (MQ=255)

   acaAACTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTGTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGAc                                                                                                                                                    <  2:4578187/150‑1 (MQ=255)

   acaAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGAc                                                                                                                                                    >  2:2665034/1‑150 (MQ=255)

     aaaaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAg                                                                                                                                                  <  1:7329799/150‑1 (MQ=255)

     aaaaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAg                                                                                                                                                  >  2:2920651/1‑150 (MQ=255)

     aaaaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGAGGGCAGGGGCCAg                                                                                                                                                  >  1:6360711/1‑150 (MQ=255)

       aaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCt                                                                                                                                                <  2:6222683/150‑1 (MQ=255)

         tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                             <  1:1541186/150‑1 (MQ=255)

         tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                             <  1:6479602/150‑1 (MQ=255)

         tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                             <  1:4680989/150‑1 (MQ=255)

         tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                             <  1:3292838/150‑1 (MQ=255)

         tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                             <  1:682014/150‑1 (MQ=255)

         tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                             <  2:6910322/150‑1 (MQ=255)

           cTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                           <  1:3469003/150‑1 (MQ=255)

             gACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGTCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCt                                                                                                                                         <  2:2681362/150‑1 (MQ=255)

              aCTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                                                                                                                        <  1:5583017/150‑1 (MQ=255)

                tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGaa                                                                                                                                      >  2:1591392/1‑150 (MQ=255)

                tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                      <  2:5112093/150‑1 (MQ=255)

                 ccGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAc                                                                                                                                     <  1:1516805/150‑1 (MQ=255)

                  cGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCt‑t                                                                                                                                              >  2:3336250/1‑140 (MQ=255)

                  cGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACt                                                                                                                                    >  2:5278961/1‑150 (MQ=255)

                  cGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTAAGGGGACAGCT‑TAGCCTGAACt                                                                                                                                    >  1:2359182/1‑150 (MQ=255)

                   gATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCGGAAcgg                                                                                                                                   >  2:3533623/1‑148 (MQ=255)

                     tGCACCGAAACGGGCCTGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                 <  1:379159/150‑1 (MQ=255)

                     tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                 <  1:4560147/150‑1 (MQ=255)

                     tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                 <  1:3426581/150‑1 (MQ=255)

                        aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTGACGCa                                                                                                                              >  1:4184813/1‑150 (MQ=255)

                         ccGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGGGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAt                                                                                                                             >  2:3501507/1‑150 (MQ=255)

                           gAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGATGCATTc                                                                                                                           <  2:3570267/150‑1 (MQ=255)

                           gAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTc                                                                                                                           <  2:6545201/150‑1 (MQ=255)

                              aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                        <  1:811573/150‑1 (MQ=255)

                               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCTATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                       <  1:4217622/150‑1 (MQ=255)

                               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                       <  1:2244126/150‑1 (MQ=21)

                               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCGGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGACTGCGTGAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                       <  2:1666942/150‑1 (MQ=21)

                                gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg                                                                                                                      <  2:6048415/150‑1 (MQ=255)

                                   ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGTCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCaa                                                                                                                   <  1:1464818/150‑1 (MQ=255)

                                   ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGcca                                                                                                                   >  2:5653923/1‑148 (MQ=255)

                                        tttGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACTTTAACCGATGATTGTGGGTTAACAATGCAAACGGGGGCTTGCCCGCGATTGCGTGCGGTCAGGGGACAGCT‑TAGCCTTAACTGACGCATTCGCGGGCAAGCCCg                                                                                                              <  2:2579606/149‑1 (MQ=255)

                                            tttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTCACAATGCAAAAGCGGGCGTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACGGCCGCCGTCGCGGGCCAGcccccgcc                                                                                                          >  2:3938585/1‑145 (MQ=255)

                                                cTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCGTTCGCGGGc                                                                                                                     <  2:575147/135‑1 (MQ=255)

                                                 tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCATGCCCCCGAATGCGTGAGGTAAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAg                                                                                                     >  1:598923/1‑150 (MQ=255)

                                                  ggCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCTGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTTTTGCatt                                                                                                    <  2:1193785/150‑3 (MQ=17)

                                                     gcCCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGCATGCGGGGGGTCAGGGGCCAGCG‑TAGCCGGCACGGACCCAATCGCGGGCCAGCCCCCGCCGGCAggggg                                                                                                 >  1:2109142/1‑150 (MQ=255)

                                                     gcCCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCCCGAAAGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACTGACGCAATAGCCGGCAAGCCCCCGCCGGCAggggg                                                                                                 >  1:2108788/1‑150 (MQ=255)

                                                     gcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAggggg                                                                                                 >  2:3935647/1‑150 (MQ=255)

                                                     gcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACCGCT‑TAGCCTGAACGGACGCATTCGCGGGCAAGCCCGCTCCTGCCggggg                                                                                                 >  2:4011147/1‑150 (MQ=255)

                                                           acccggtgGGGGGCGGGTGACACAACATTACCCTTTGATTGTCGGTTAAAAATGAAAACGCGGGCTTGCCCGCGATTGCGTGTGGTCCGGGGAAAACT‑TAGCCTCAACGGCCGCTTTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGc                                                                                           <  2:5387405/145‑1 (MQ=255)

                                                                 tgCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCGCCTGCAGGGGGTAGTGCCgggcc                                                                                     >  2:6582317/1‑148 (MQ=255)

                                                                     gggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAATGCGGGCGGTGGGGGGACCCCG‑TAGCCGGAACGGGCGCAGTCGCGGGCCAGCCCCCGCCGGCCGGGGGGAGTGGCGGGGAgggg                                                                                 >  1:6563764/1‑149 (MQ=255)

                                                                          ggTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTACCCCGAAATCGTGTGGTGAGGGGACAGCA‑TATCCTGTAAGGAAGCAATAGCGGGCAACCCCGCTCCTGCAGGGGGGGTTTGCGGGGAGGGTTTaag                                                                            >  1:4585621/1‑148 (MQ=255)

                                                                           gTGACAGAACAGTAAGCGATGATTGTCGGGTAACAATGCAAATGCGGGCTTGGGCGCGTATGGGTGAGGTGGGGGGTCAGGT‑TTGCCTGTAGTGACGGATTGGGGGGCAAGGCCGCTGCTGGAGGGGGTTTTGGGGGGGAGGGttttagg                                                                           >  2:1041355/1‑146 (MQ=255)

                                                                           gTGACAGAACAGTAAGCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTTCCCGCGAATGCGTGTGGTGAGGGGACAGGT‑TTGGGTGTAGTGTCGCATTGGCGGGCAAGCCCGCTCGTGGAGGGGGTTTTGGCGGGTAGGGTTTAAGc                                                                           >  2:1041336/1‑150 (MQ=255)

                                                                                aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCAGCAGGGGGTATTGCCGGGCAGGGTTTACGCCGAAg                                                                      >  2:5110588/1‑150 (MQ=255)

                                                                                aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTGACGCATTCGCGGGCAAGCCCCCTCCTGCAGGGGGTATTTCCGGGCAGGGTTTACGCCGCAg                                                                      >  2:1041332/1‑150 (MQ=255)

                                                                                       tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCCCCAAAGCCGGAGGTCAGGGGACCCCG‑TAGCCTGAACGGACCCAATCGCGGGCCACCCCCCTCCAGCAGGGGGTATTGCCGGGCAGGGGTTACGCCCAAGCCACGtt                                                               >  2:48115/1‑150 (MQ=255)

                                                                                        aaCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGCGGTCCGGGGCCAGCT‑TAGCCGGAACGGACCCATTCGCGGGCAAGCCCGCTCCTGCCGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCCCGTTc                                                              >  1:4211405/1‑150 (MQ=255)

                                                                                        aaCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTCCCCCGCAAGCGTGAGGTCAGGGGACAGCA‑TAGCCGGAACAGACCCAATAGCGGGCAAGCCCGCACCGGCAGGGGGTATTTCCGGGAAGGGTTTAACCCGAACCCACGttg                                                              >  1:2883454/1‑149 (MQ=255)

                                                                                            gatgatTGTCGGGTAACAATGCAAATGCGGGCTTGCCCGCGAATTGGTGTGGTGAGGGGTCAGGT‑TAGGGTGAAATGACGCATTCGCGGGCAAGCCCGCTGCTGGAGGGGGTATTGCCGGGTAGGGTTTACGCCGAAGCCAGGTTAAtgg                                                          >  2:837664/1‑148 (MQ=255)

                                                                                            gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCCAAAGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAAGCATTCGCGGGCAAGCCCGCTCCAGCAGGGGGGATTGCCGGGAACGGTTTACGCCGAAGCCAGGTTCATCg                                                          >  2:3881478/1‑150 (MQ=255)

                                                                                            gatgatTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAATGCGTGAGGTCAGGGGACCGCG‑TAGCCTGAACTGACCCATTCGCGGGCAAGCCCGCTCCAGCAGGGGGTATTGCCGGGACGGGTTTACGCCGAAGCCAGGTTCATCg                                                          >  1:5452051/1‑150 (MQ=255)

                                                                                                       ggTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGGCAGCT‑TAGCCGGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTa                                               >  2:1708662/1‑150 (MQ=255)

                                                                                                            cAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGAGGTCAGGGGACAGCT‑TAGCCAGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGAt                                          >  2:3711072/1‑150 (MQ=255)

                                                                                                                  attcgCGGGCAAGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                    >  1:6185254/5‑150 (MQ=255)

                                                                                                                  attcgCGGGCAAGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                    >  1:6184767/5‑150 (MQ=255)

                                                                                                                  aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                    >  2:7399779/1‑150 (MQ=255)

                                                                                                                  aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                    >  2:6943031/1‑150 (MQ=255)

                                                                                                                  aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACAGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGACAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                    >  2:2230659/1‑150 (MQ=255)

                                                                                                                          gCTTGCCCGCTAATGCGTGAGGTCTGGGGACAGCT‑TCGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                            <  2:3656023/150‑1 (MQ=255)

                                                                                                                          gCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                            >  2:4530282/1‑150 (MQ=255)

                                                                                                                            ttGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAGTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcaca                          >  1:6767963/1‑150 (MQ=255)

                                                                                                                              gCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAcc                        >  1:169441/1‑150 (MQ=255)

                                                                                                                                ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                      >  2:2332395/1‑150 (MQ=255)

                                                                                                                                ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                      >  2:1172602/1‑150 (MQ=255)

                                                                                                                                ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                      >  2:5362765/1‑150 (MQ=255)

                                                                                                                                ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTTCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATc                                                           >  2:3422029/1‑113 (MQ=11)

                                                                                                                                ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTTCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATc                                                           <  1:3422029/113‑1 (MQ=11)

                                                                                                                                ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                      >  1:5944621/1‑150 (MQ=21)

                                                                                                                                ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                      >  1:673600/1‑150 (MQ=21)

                                                                                                                                 cgcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                     >  2:468615/1‑150 (MQ=255)

                                                                                                                                 cgcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                     >  1:4631461/1‑150 (MQ=21)

                                                                                                                                  gcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTTTTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                    >  2:3477241/1‑150 (MQ=18)

                                                                                                                                    gAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTc                  >  1:3903317/1‑150 (MQ=255)

                                                                                                                                         cGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCa             >  1:1982791/1‑150 (MQ=255)

                                                                                                                                          gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa            >  1:5244367/1‑150 (MQ=255)

                                                                                                                                          gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa            >  1:3237932/1‑150 (MQ=255)

                                                                                                                                          gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa            >  1:5146179/1‑150 (MQ=255)

                                                                                                                                          gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa            >  2:6599351/1‑150 (MQ=255)

                                                                                                                                            gAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAgg          >  1:114090/1‑150 (MQ=255)

                                                                                                                                             aGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGt         >  1:2778311/1‑150 (MQ=255)

                                                                                                                                              ggTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTc        <  1:6599351/150‑1 (MQ=255)

                                                                                                                                                 cAGGGTACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgag     <  2:4846170/150‑1 (MQ=255)

                                                                                                                                                 cAGGGGACCGCT‑TAGCCTTAACTGACGCATTCGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgag     <  2:4846096/150‑1 (MQ=255)

                                                                                                                                                  tggggACAGCT‑TCGCCTTAACTGCCGCATTCGGGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga    <  2:4607456/149‑1 (MQ=255)

                                                                                                                                                  aGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga    >  1:2745502/1‑150 (MQ=255)

                                                                                                                                                  aGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga    >  1:6082314/1‑150 (MQ=255)

                                                                                                                                                  aGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga    >  1:5561040/1‑150 (MQ=255)

                                                                                                                                                    gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata  >  2:2633704/1‑150 (MQ=255)

                                                                                                                                                    gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata  >  1:2891907/1‑150 (MQ=255)

                                                                                                                                                    gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata  >  1:2891933/1‑150 (MQ=255)

TGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATA  >  NC_002947/5102079‑5102376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`