BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,102,238	G→C	8.9%	intergenic (+232/+60)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,102,238	0	G	C	8.9%	398.1 / 26.7	135	intergenic (+232/+60)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base G (89/33); new base C (7/5); total (97/38)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01

Only 100 of 138 total aligned reads displayed.

CTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGC  >  NC_002947/5102090‑5102387

cTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                                      <  1:3469003/150‑1 (MQ=255)

  gACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGTCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCt                                                                                                                                                    <  2:2681362/150‑1 (MQ=255)

   aCTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                                                                                                                                   <  2:1946024/150‑1 (MQ=255)

     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGaa                                                                                                                                                 >  2:1591392/1‑150 (MQ=255)

     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                 <  2:5112093/150‑1 (MQ=255)

      ccGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAc                                                                                                                                                <  1:1516805/150‑1 (MQ=255)

       cGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACt                                                                                                                                               >  2:5278961/1‑150 (MQ=255)

       cGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTAAGGGGACAGCT‑TAGCCTGAACt                                                                                                                                               >  1:2359182/1‑150 (MQ=255)

        gATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCGGAAcgg                                                                                                                                              >  2:3533623/1‑148 (MQ=255)

          tGCACCGAAACGGGCCTGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                            <  1:379159/150‑1 (MQ=255)

          tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                            <  2:4801807/150‑1 (MQ=255)

          tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                            <  1:4560147/150‑1 (MQ=255)

          tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                            <  1:3426581/150‑1 (MQ=255)

             aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTGACGCa                                                                                                                                         >  1:4184813/1‑150 (MQ=255)

              ccGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGGGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAt                                                                                                                                        >  2:3501507/1‑150 (MQ=255)

                gAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTc                                                                                                                                      <  2:6545201/150‑1 (MQ=255)

                  aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgc                                                                                                                                    <  1:6129184/150‑1 (MQ=255)

                   aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                   <  1:811573/150‑1 (MQ=255)

                    cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCTATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                  <  1:4217622/150‑1 (MQ=255)

                    cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                  <  2:6234433/150‑1 (MQ=255)

                    cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                                  <  1:2244126/150‑1 (MQ=21)

                    cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCGGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGACTGCGTGAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                                  <  2:1666942/150‑1 (MQ=21)

                     gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg                                                                                                                                 <  2:6048415/150‑1 (MQ=255)

                        ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGTCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCaa                                                                                                                              <  1:1464818/150‑1 (MQ=255)

                        ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGcca                                                                                                                              >  2:5653923/1‑148 (MQ=255)

                         cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGAGGTCAGGGGCCAGCT‑TTGCCGGTCCGGACGCATTCGCGGGCacg                                                                                                                             >  1:6745363/1‑148 (MQ=255)

                             tttGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACTTTAACCGATGATTGTGGGTTAACAATGCAAACGGGGGCTTGCCCGCGATTGCGTGCGGTCAGGGGACAGCT‑TAGCCTTAACTGACGCATTCGCGGGCAAGCCCg                                                                                                                         <  2:2579606/149‑1 (MQ=255)

                                 tttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGTGCAAGccaccacc                                                                                                                     >  2:1082530/1‑144 (MQ=18)

                                  ttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGGCAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCGccg                                                                                                                    >  1:3656023/1‑149 (MQ=255)

                                     cTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCGTTCGCGGGc                                                                                                                                <  2:575147/135‑1 (MQ=255)

                                     cTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCGTTCGCGGGc                                                                                                                                >  1:575147/1‑135 (MQ=255)

                                      tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAg                                                                                                                >  2:4894390/1‑150 (MQ=255)

                                      tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCATGCCCCCGAATGCGTGAGGTAAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAg                                                                                                                >  1:598923/1‑150 (MQ=255)

                                       ggCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCTGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTTTTGCatt                                                                                                               <  2:1193785/150‑3 (MQ=17)

                                          gcCCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGCATGCGGGGGGTCAGGGGCCAGCG‑TAGCCGGCACGGACCCAATCGCGGGCCAGCCCCCGCCGGCAggggg                                                                                                            >  1:2109142/1‑150 (MQ=255)

                                          gcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTGCCCGCGGATGCGTGTGGTGAGGGGTCAGCG‑TAGCCGGAACGGTCGCATTCGCGGGCCACCCCGCGCCGGCgggggg                                                                                                            >  1:843405/1‑150 (MQ=255)

                                          gcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTGAGGGGGCACCG‑TAGCCAGGACTGACGCAGTCGCGGGCAAGCCCGCGCCGGCAggggg                                                                                                            >  1:2669248/1‑150 (MQ=255)

                                          gcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAggggg                                                                                                            >  2:3935647/1‑150 (MQ=255)

                                          gcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACCGCT‑TAGCCTGAACGGACGCATTCGCGGGCAAGCCCGCTCCTGCCggggg                                                                                                            >  2:4011147/1‑150 (MQ=255)

                                                          gggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAATGCGGGCGGTGGGGGGACCCCG‑TAGCCGGAACGGGCGCAGTCGCGGGCCAGCCCCCGCCGGCCGGGGGGAGTGGCGGGGAgggg                                                                                            >  1:6563764/1‑149 (MQ=255)

                                                               ggTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTACCCCGAAATCGTGTGGTGAGGGGACAGCA‑TATCCTGTAAGGAAGCAATAGCGGGCAACCCCGCTCCTGCAGGGGGGGTTTGCGGGGAGGGTTTaag                                                                                       >  1:4585621/1‑148 (MQ=255)

                                                                gTGACAGAACAGTAAGCGATGATTGTCGGGTAACAATGCAAATGCGGGCTTGGGCGCGTATGGGTGAGGTGGGGGGTCAGGT‑TTGCCTGTAGTGACGGATTGGGGGGCAAGGCCGCTGCTGGAGGGGGTTTTGGGGGGGAGGGttttagg                                                                                      >  2:1041355/1‑146 (MQ=255)

                                                                gTGACAGAACAGTAAGCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTTCCCGCGAATGCGTGTGGTGAGGGGACAGGT‑TTGGGTGTAGTGTCGCATTGGCGGGCAAGCCCGCTCGTGGAGGGGGTTTTGGCGGGTAGGGTTTAAGc                                                                                      >  2:1041336/1‑150 (MQ=255)

                                                                     aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCAGCAGGGGGTATTGCCGGGCAGGGTTTACGCCGAAg                                                                                 >  2:5110588/1‑150 (MQ=255)

                                                                            tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCCCCAAAGCCGGAGGTCAGGGGACCCCG‑TAGCCTGAACGGACCCAATCGCGGGCCACCCCCCTCCAGCAGGGGGTATTGCCGGGCAGGGGTTACGCCCAAGCCACGtt                                                                          >  2:48115/1‑150 (MQ=255)

                                                                             aaCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGCGGTCCGGGGCCAGCT‑TAGCCGGAACGGACCCATTCGCGGGCAAGCCCGCTCCTGCCGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCCCGTTc                                                                         >  1:4211405/1‑150 (MQ=255)

                                                                             aaCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTCCCCCGCAAGCGTGAGGTCAGGGGACAGCA‑TAGCCGGAACAGACCCAATAGCGGGCAAGCCCGCACCGGCAGGGGGTATTTCCGGGAAGGGTTTAACCCGAACCCACGttg                                                                         >  1:2883454/1‑149 (MQ=255)

                                                                               ccGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCAAGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCACCAGCAGGGGGGATTGCCGGGAAGGGTTTACGCCGAAGCCAGGttaaa                                                                       >  1:1732435/1‑147 (MQ=255)

                                                                                 gatgatTGTCGGGTAACAATGCAAATGCGGGCTTGCCCGCGAATTGGTGTGGTGAGGGGTCAGGT‑TAGGGTGAAATGACGCATTCGCGGGCAAGCCCGCTGCTGGAGGGGGTATTGCCGGGTAGGGTTTACGCCGAAGCCAGGTTAAtgg                                                                     >  2:837664/1‑148 (MQ=255)

                                                                                            ggTAACAATGCAAAAGCGGGCTTGCCCCCGCATGCGTGAGGTCAGGGGACAGCG‑TAGCCGGAACTGACGCATTCGCGGGCAAGCCCGCTCCGGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGGTCATCGCCGTGTgggga                                                          >  2:3534686/1‑148 (MQ=255)

                                                                                                 cAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGAGGTCAGGGGACAGCT‑TAGCCAGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGAt                                                     >  2:3711072/1‑150 (MQ=255)

                                                                                                    tGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCAGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTCGTGGGTATCGATCAg                                                  >  2:4269643/1‑150 (MQ=255)

                                                                                                     gCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGGATCGATCAgg                                                 >  2:6781936/1‑150 (MQ=255)

                                                                                                       attcgCGGGCAAGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                               >  1:6185254/5‑150 (MQ=255)

                                                                                                       aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                               >  2:7399779/1‑150 (MQ=255)

                                                                                                       aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                               >  2:4596533/1‑150 (MQ=255)

                                                                                                       aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                               >  2:6943031/1‑150 (MQ=255)

                                                                                                       aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                               >  2:3665459/1‑150 (MQ=255)

                                                                                                       aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACAGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGACAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                               >  2:2230659/1‑150 (MQ=255)

                                                                                                         aaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCt                                             >  2:5540351/1‑150 (MQ=255)

                                                                                                         aaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCt                                             >  2:3427650/1‑150 (MQ=255)

                                                                                                               gCTTGCCCGCTAATGCGTGAGGTCTGGGGACAGCT‑TCGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                                       <  2:3656023/150‑1 (MQ=255)

                                                                                                                 ttGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAGTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcaca                                     >  1:6767963/1‑150 (MQ=255)

                                                                                                                     ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                                 >  2:5362765/1‑150 (MQ=255)

                                                                                                                     ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTTCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATc                                                                      <  1:3422029/113‑1 (MQ=11)

                                                                                                                     ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                                 >  1:673600/1‑150 (MQ=21)

                                                                                                                     ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                                 >  1:5476414/1‑150 (MQ=21)

                                                                                                                      cgcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                                >  1:4631461/1‑150 (MQ=21)

                                                                                                                       gctattGCGTGTGGTCTGGGGACAGCT‑TAGCCTGAACTGACGCTTTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                               <  2:5974859/145‑1 (MQ=255)

                                                                                                                       gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                               >  2:3158651/1‑150 (MQ=255)

                                                                                                                       gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                               <  1:3158651/150‑1 (MQ=255)

                                                                                                                         gAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTc                             >  1:3903317/1‑150 (MQ=255)

                                                                                                                              cGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCa                        >  1:1982791/1‑150 (MQ=255)

                                                                                                                               gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                       >  2:6599351/1‑150 (MQ=255)

                                                                                                                               gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                       >  1:5146069/1‑150 (MQ=255)

                                                                                                                               gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                       >  2:254331/1‑150 (MQ=255)

                                                                                                                               gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                       >  1:5146179/1‑150 (MQ=255)

                                                                                                                               gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                       >  1:5244367/1‑150 (MQ=255)

                                                                                                                                 gAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAgg                     >  1:114090/1‑150 (MQ=255)

                                                                                                                                  aGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGt                    >  1:2778311/1‑150 (MQ=255)

                                                                                                                                   ggTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTc                   <  1:6599351/150‑1 (MQ=255)

                                                                                                                                      cAGGGGACCGCT‑TAGCCTTAACTGACGCATTCGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgag                <  2:4846096/150‑1 (MQ=255)

                                                                                                                                       tggggACAGCT‑TCGCCTTAACTGCCGCATTCGGGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga               <  2:4607456/149‑1 (MQ=255)

                                                                                                                                       aGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga               >  1:5561040/1‑150 (MQ=255)

                                                                                                                                       aGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga               >  1:6082314/1‑150 (MQ=255)

                                                                                                                                         gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata             >  1:2891907/1‑150 (MQ=255)

                                                                                                                                         gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata             >  2:3907157/1‑150 (MQ=255)

                                                                                                                                         gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata             >  2:2633704/1‑150 (MQ=255)

                                                                                                                                           gACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATc           >  1:3048648/1‑150 (MQ=255)

                                                                                                                                             ctgCT‑TAGCCTGACCTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGc         <  2:4184813/148‑1 (MQ=255)

                                                                                                                                              tgCT‑TTGCCTGACCTGACGCTTGCGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGcc        <  2:3372035/149‑1 (MQ=255)

                                                                                                                                              cgCT‑TAGCCTGACCGGACGCTTGCGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGcc        <  2:3447400/149‑1 (MQ=255)

                                                                                                                                                cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa      >  1:2189812/1‑150 (MQ=255)

                                                                                                                                                cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa      >  1:6412186/1‑150 (MQ=255)

                                                                                                                                                 t‑tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg     >  1:6294575/1‑150 (MQ=255)

                                                                                                                                                 t‑tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg     >  1:4816904/1‑150 (MQ=255)

                                                                                                                                                 t‑tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg     >  1:5550615/1‑150 (MQ=255)

                                                                                                                                                     gCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                               <  2:5616151/105‑1 (MQ=255)

                                                                                                                                                     gCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                               >  1:5616151/1‑105 (MQ=255)

                                                                                                                                                     gCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGc  >  1:1958872/1‑150 (MQ=255)

CTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGC  >  NC_002947/5102090‑5102387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`