BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,186,149	A→T	6.2%	L264Q (CTG→CAG)	PP_4560 ←	ribonuclease BN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,186,149	0	A	T	6.2%	941.7 / 34.8	274	L264Q (CTG→CAG)	PP_4560	ribonuclease BN
Reads supporting (aligned to +/- strand): ref base A (126/131); new base T (7/10); total (133/141)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 288 total aligned reads displayed.

GCTATGAGGCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGCCAGCACCGAGC  >  NC_002947/5186001‑5186296

gCTATGAGGCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGagcagcag                                                                                                                                                    >  2:3570536/1‑150 (MQ=255)

  tATGAGGCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAgcagcagca                                                                                                                                                  >  1:3550281/1‑150 (MQ=255)

       ggCGTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCa                                                                                                                                             <  1:5437261/150‑1 (MQ=255)

          ttGCTCGGGTTTGGGTGCTCTTGACCCAGTAAAGTCAAGGTTTACTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GACGAGCAGCAGAACGGCAGcc                                                                                                                                          >  1:279211/1‑150 (MQ=255)

          ttGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGcc                                                                                                                                          <  1:452335/150‑1 (MQ=255)

              tCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGaaa                                                                                                                                      >  1:5886142/1‑150 (MQ=255)

              tCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGaaa                                                                                                                                      >  2:5599898/1‑150 (MQ=255)

              tCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTGTCCCCGTCGAAGTCTTTCTCGCCCCGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGaaa                                                                                                                                      <  2:4682567/150‑1 (MQ=255)

               cGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAat                                                                                                                                     <  2:5719644/150‑1 (MQ=255)

                 ggTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGCTGCCGTGCTGCTGCTCGGC‑GCCGAAatga                                                                                                                                   <  1:5395643/150‑3 (MQ=38)

                 ggTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGCTGCCGTGCTGCTGCTCGGC‑GCCGAAatga                                                                                                                                   <  1:4878134/150‑3 (MQ=38)

                  gTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGCTGCCGTGCTGCTGCTCGGC‑GCCGAAatgaa                                                                                                                                  <  2:6089476/150‑4 (MQ=38)

                  gTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAAtata                                                                                                                                  <  2:6030632/150‑1 (MQ=255)

                     tGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGCTGCCGTGCTGCTGCTCGGC‑GCCGAAatgaatgc                                                                                                                               <  1:2950416/150‑7 (MQ=38)

                     tGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGaa                                                                                                                               <  2:3684767/150‑1 (MQ=255)

                     tGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGaa                                                                                                                               <  2:7419877/150‑1 (MQ=255)

                      gggTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGaaa                                                                                                                              <  2:2472833/150‑1 (MQ=255)

                      gggTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGaaa                                                                                                                              >  2:2061649/1‑150 (MQ=255)

                        gTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATa                                                                                                                            >  1:4216105/1‑150 (MQ=255)

                         tGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATac                                                                                                                           >  2:3499430/1‑150 (MQ=255)

                         tGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATac                                                                                                                           >  1:5970311/1‑150 (MQ=255)

                              gTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATacagcaa                                                                                                                      >  1:499475/1‑150 (MQ=255)

                               tGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATacagcaac                                                                                                                     >  2:4354361/1‑150 (MQ=255)

                                  cccAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTACGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATacagcaacagc                                                                                                                  >  1:1067276/1‑150 (MQ=255)

                                       tACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACgat                                                                                                             >  2:6344502/1‑150 (MQ=255)

                                         cAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACgatga                                                                                                           <  2:197031/150‑1 (MQ=255)

                                           gTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGAtt                                                                                                         <  2:2225756/150‑1 (MQ=255)

                                            tCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTg                                                                                                        >  1:6531409/1‑150 (MQ=255)

                                            tCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTg                                                                                                        >  1:3720968/1‑150 (MQ=255)

                                            tCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTg                                                                                                        >  1:4755430/1‑150 (MQ=255)

                                            tCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTg                                                                                                        <  1:1608273/150‑1 (MQ=255)

                                              aaGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCa                                                                                                      <  1:4228671/150‑1 (MQ=255)

                                               aGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCAc                                                                                                     <  2:4389553/150‑1 (MQ=255)

                                                ggTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATaca                                                                                                                          <  1:5243143/128‑1 (MQ=255)

                                                ggTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCAcc                                                                                                    <  1:103688/150‑1 (MQ=255)

                                                  tttCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCaa                                                                                                  >  2:440589/1‑150 (MQ=255)

                                                  tttCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCaa                                                                                                  <  1:1569281/150‑1 (MQ=255)

                                                  tttCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCaa                                                                                                  >  1:3304607/1‑150 (MQ=255)

                                                    tCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAAtg                                                                                                <  2:6830229/150‑1 (MQ=255)

                                                    tCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAAtg                                                                                                <  1:1464413/150‑1 (MQ=255)

                                                     ccTTGGGCTGGTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAAggc                                                                                               <  2:4062794/150‑1 (MQ=255)

                                                      cTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAAtgct                                                                                              >  2:5785603/1‑150 (MQ=255)

                                                      cTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAAtgct                                                                                              >  1:821295/1‑150 (MQ=255)

                                                          ggCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCg                                                                                          >  1:6627700/1‑150 (MQ=255)

                                                            cTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTa                                                                                        <  2:6515570/150‑1 (MQ=255)

                                                             ttttCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTAc                                                                                       >  2:844588/1‑150 (MQ=255)

                                                                 ccccGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATgg                                                                                   >  2:1794106/1‑150 (MQ=255)

                                                                  cccGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGc                                                                                  <  2:3304607/150‑1 (MQ=255)

                                                                  cccGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGc                                                                                  >  2:4566489/1‑150 (MQ=255)

                                                                   ccGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCg                                                                                 <  1:1326287/150‑1 (MQ=255)

                                                                    cGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGt                                                                                <  2:1131020/150‑1 (MQ=255)

                                                                    cGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGt                                                                                <  2:5106802/150‑1 (MQ=255)

                                                                     gTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGtt                                                                               <  1:2114614/150‑1 (MQ=255)

                                                                     gTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGtt                                                                               <  2:2581407/150‑1 (MQ=255)

                                                                      tCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTg                                                                              <  1:6097818/150‑1 (MQ=255)

                                                                        gAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTa                                                                            <  1:2892863/150‑1 (MQ=255)

                                                                              tttCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGc                                                                      >  1:2387243/1‑150 (MQ=255)

                                                                                  tcGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAg                                                                  >  1:5115414/1‑150 (MQ=255)

                                                                                    gCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGt                                                                >  2:6233182/1‑150 (MQ=255)

                                                                                    gCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGt                                                                >  1:5739951/1‑150 (MQ=255)

                                                                                     cccGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTc                                                               <  1:5709189/150‑1 (MQ=255)

                                                                                        gggTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTc                                                            >  1:2984432/1‑150 (MQ=255)

                                                                                         ggTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCa                                                           <  1:6233182/150‑1 (MQ=255)

                                                                                         ggTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCa                                                           >  2:1608133/1‑150 (MQ=255)

                                                                                           ttCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACg                                                         >  1:4022339/1‑150 (MQ=255)

                                                                                             cTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACgta                                                       >  2:1459376/1‑150 (MQ=255)

                                                                                                 ccTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGtagtag                                                   <  2:2687387/150‑1 (MQ=255)

                                                                                                 ccTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGtagtag                                                   >  2:789710/1‑150 (MQ=255)

                                                                                                  cTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAgg                                                  >  1:2327048/1‑150 (MQ=255)

                                                                                                   ttCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGc                                                 >  1:4472074/1‑150 (MQ=255)

                                                                                                         tGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGcc                                           >  1:1720286/1‑150 (MQ=255)

                                                                                                          gACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGccc                                          <  1:4133355/150‑1 (MQ=255)

                                                                                                           aCATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCa                                         >  2:3376565/1‑150 (MQ=255)

                                                                                                             aTGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAgg                                       <  1:6785676/150‑1 (MQ=255)

                                                                                                                tgCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACTGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAg                                    <  2:6728830/150‑1 (MQ=255)

                                                                                                                tgCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAg                                    <  2:5441183/150‑1 (MQ=255)

                                                                                                                  cTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGc                                  <  2:4787852/150‑1 (MQ=255)

                                                                                                                   tCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACgtagta                                                    <  2:2328254/131‑1 (MQ=255)

                                                                                                                    cGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCa                                >  2:2880771/1‑150 (MQ=255)

                                                                                                                    cGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCa                                <  2:3173453/150‑1 (MQ=255)

                                                                                                                       tCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGa                             >  1:857824/1‑150 (MQ=255)

                                                                                                                       tCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGa                             <  1:6304485/150‑1 (MQ=255)

                                                                                                                         aCCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATc                           <  1:3848058/150‑1 (MQ=255)

                                                                                                                          ccGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATcc                          >  2:5343886/1‑150 (MQ=255)

                                                                                                                          ccGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATcc                          >  1:761001/1‑150 (MQ=255)

                                                                                                                            gCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACATCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATccac                        <  1:2773748/150‑1 (MQ=255)

                                                                                                                              attcattTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATccacac                      <  1:5599898/150‑1 (MQ=255)

                                                                                                                              attcattTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATccacac                      <  1:1897900/150‑1 (MQ=255)

                                                                                                                               ttcattTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATccacacc                     >  1:727559/1‑150 (MQ=255)

                                                                                                                                tcattTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATccacacca                    >  1:7002758/1‑150 (MQ=255)

                                                                                                                                  attTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATccacaccaca                  >  2:3848651/1‑150 (MQ=255)

                                                                                                                                        gctgcCGTGCTGCTGCTCGGC‑GCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGcc            >  1:2507852/4‑150 (MQ=255)

                                                                                                                                        gctgcCGTGCTGCTGCTCGGC‑GCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGcc            >  2:715225/4‑150 (MQ=255)

                                                                                                                                        gctgcCGTGCTGCTGCTCGGC‑GCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGcc            >  2:4276721/4‑150 (MQ=255)

                                                                                                                                        gc‑gcCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGcc            >  1:2266329/1‑150 (MQ=255)

                                                                                                                                            ccGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGCCAGc         <  1:4370026/150‑1 (MQ=255)

                                                                                                                                              gAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACgtagt                                                     <  2:7349792/104‑1 (MQ=255)

                                                                                                                                                gcagcagcaCGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGCCAGCACCg     <  2:5886118/150‑1 (MQ=255)

                                                                                                                                                  agcagcaCGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACg                                                         <  2:688109/96‑1 (MQ=255)

                                                                                                                                                   gcagcaCGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGCCAGCACCGAGc  >  2:3900041/1‑150 (MQ=255)

GCTATGAGGCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGCCAGCACCGAGC  >  NC_002947/5186001‑5186296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`