BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,192,980	G→A	5.8%	R163H (CGC→CAC) ‡	PP_4567 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,192,980	0	G	A	5.8%	1155.6 / 39.0	360	R163H (CGC→CAC) ‡	PP_4567	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (185/154); new base A (14/7); total (199/161)
Fisher's exact test for biased strand distribution p-value = 3.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 387 total aligned reads displayed.

CTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCAGCAAGTCC  >  NC_002947/5192832‑5193129

cTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTgcgc                                                                                                                                                      >  1:110709/1‑150 (MQ=255)

cTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTgcgc                                                                                                                                                      <  1:5485075/150‑1 (MQ=255)

cTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTgcgc                                                                                                                                                      >  1:150430/1‑150 (MQ=255)

 tGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGcgcc                                                                                                                                                     >  2:836055/1‑150 (MQ=255)

     tttCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGcgccacgc                                                                                                                                                 <  2:1541606/150‑1 (MQ=255)

         ggCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATc                                                                                                                                             <  2:3026617/150‑1 (MQ=255)

             gcgcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCCGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCg                                                                                                                                         <  2:4458669/150‑1 (MQ=255)

               gcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGaa                                                                                                                                       <  1:4546851/150‑1 (MQ=255)

               gcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGaa                                                                                                                                       <  1:5832983/150‑1 (MQ=255)

                 cTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAaga                                                                                                                                     >  2:2097050/1‑150 (MQ=255)

                  tgtgAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGGGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAagag                                                                                                                                    >  1:6900749/1‑150 (MQ=255)

                    tgAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGtt                                                                                                                                  >  1:6472091/1‑150 (MQ=255)

                    tgAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTAGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACTCCATCCCCGAAGAGtt                                                                                                                                  >  1:92033/1‑150 (MQ=255)

                    cgAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGtt                                                                                                                                  >  1:3901404/2‑150 (MQ=255)

                     gAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTc                                                                                                                                 >  2:4759362/1‑150 (MQ=255)

                      aaaCCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTcc                                                                                                                                <  2:7429545/150‑1 (MQ=255)

                      aaaCCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTcc                                                                                                                                >  1:5194966/1‑150 (MQ=255)

                      aaaCCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTcc                                                                                                                                <  2:2519375/150‑1 (MQ=255)

                       aaCCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCg                                                                                                                               <  2:3207566/150‑1 (MQ=255)

                           gAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAg                                                                                                                           >  1:2236882/1‑150 (MQ=255)

                           gAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAg                                                                                                                           >  1:2072655/1‑150 (MQ=255)

                                  cAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTAc                                                                                                                    >  1:6165793/1‑150 (MQ=255)

                                    gTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGc                                                                                                                  <  1:4423755/150‑1 (MQ=255)

                                     tGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCt                                                                                                                 <  2:4088452/150‑1 (MQ=255)

                                      gAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCtt                                                                                                                >  1:7397912/1‑150 (MQ=255)

                                       aaGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTc                                                                                                               >  2:664382/1‑150 (MQ=255)

                                       aaGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTc                                                                                                               >  1:2791949/1‑150 (MQ=255)

                                          aTGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGc                                                                                                            <  1:3398584/150‑1 (MQ=255)

                                          aTGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGc                                                                                                            <  2:5851681/150‑1 (MQ=255)

                                                gCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGgcg                                                                                                      >  2:6172018/1‑150 (MQ=255)

                                                 cGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGgcgc                                                                                                     <  1:3174752/150‑1 (MQ=255)

                                                  ggTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGgcgcg                                                                                                    >  1:4879754/1‑150 (MQ=255)

                                                  ggTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGgcgcg                                                                                                    >  1:1141586/1‑150 (MQ=255)

                                                   gTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGgcgcgc                                                                                                   >  1:4412458/1‑150 (MQ=255)

                                                   gTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCGCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGgcgcgc                                                                                                   <  2:6524528/150‑1 (MQ=255)

                                                        ggTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGcggcgg                                                                                              <  2:5379404/150‑1 (MQ=255)

                                                        ggTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGcggcgg                                                                                              <  1:4085842/150‑1 (MQ=255)

                                                         ggcccGCCGCAACGCTCGCTCGATACCCTGCGCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTGCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCggg                                                                                             >  1:2572166/3‑150 (MQ=255)

                                                         gTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCggg                                                                                             >  2:1165286/1‑150 (MQ=255)

                                                            ccgccgCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCtt                                                                                          >  1:6624862/1‑150 (MQ=255)

                                                            ccgccgCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCtt                                                                                          >  2:4658381/1‑150 (MQ=255)

                                                            ccgccgCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCtt                                                                                          >  1:5944521/1‑150 (MQ=255)

                                                             cgccgcAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTg                                                                                         <  1:6206755/150‑1 (MQ=255)

                                                               ccgcAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCg                                                                                       <  2:4922777/150‑1 (MQ=255)

                                                                cgcAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGa                                                                                      <  2:2787378/150‑1 (MQ=255)

                                                                cgcAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAACCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGa                                                                                      <  1:1817270/150‑1 (MQ=255)

                                                                 gcAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAgg                                                                                                                          >  2:4850231/1‑113 (MQ=255)

                                                                 gcAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGaa                                                                                     >  2:449518/1‑150 (MQ=255)

                                                                  cAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGaaa                                                                                    <  2:5979244/150‑1 (MQ=255)

                                                                  cAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGaaa                                                                                    <  1:2680122/150‑1 (MQ=255)

                                                                       ctcgctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGa                                                                               >  2:3889703/1‑150 (MQ=255)

                                                                        tcgctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGaa                                                                              <  2:5277583/150‑1 (MQ=255)

                                                                        tcgctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGaa                                                                              <  1:3072999/150‑1 (MQ=255)

                                                                          gctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAca                                                                            <  1:166059/150‑1 (MQ=255)

                                                                            tcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcagc                                                                          <  2:1019283/150‑1 (MQ=255)

                                                                            tcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcagc                                                                          >  1:1389647/1‑150 (MQ=255)

                                                                            tcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcagc                                                                          >  1:2422836/1‑150 (MQ=255)

                                                                            tcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcaga                                                                          >  1:5215710/1‑149 (MQ=255)

                                                                              gATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcagcag                                                                        >  1:5007848/1‑150 (MQ=255)

                                                                                   ccTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTa                                                                   >  2:7199603/1‑150 (MQ=255)

                                                                                        tctACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACg                                                              >  1:6368980/1‑150 (MQ=255)

                                                                                        tctACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACg                                                              >  2:2669391/1‑150 (MQ=255)

                                                                                        tctACTACCTGCTCACCCAGCGCGAAGCAGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACg                                                              >  1:2967422/1‑150 (MQ=255)

                                                                                              acCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGc                                                        <  1:724590/150‑1 (MQ=255)

                                                                                                cTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGccc                                                      >  2:5172701/1‑150 (MQ=255)

                                                                                                        ccAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCca                                              >  1:1965070/1‑150 (MQ=255)

                                                                                                        ccAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCca                                              >  1:3261045/1‑150 (MQ=255)

                                                                                                         cAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcag                                             >  2:189685/1‑150 (MQ=255)

                                                                                                          aGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcagc                                            >  2:7260441/1‑150 (MQ=255)

                                                                                                           gcgcGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcagca                                           <  2:5055246/150‑1 (MQ=255)

                                                                                                           gcgcGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcagca                                           >  2:4951781/1‑150 (MQ=255)

                                                                                                           gcgcGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcagca                                           <  1:5288761/150‑1 (MQ=255)

                                                                                                            cgcgAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcagcag                                          >  2:6969867/1‑150 (MQ=255)

                                                                                                            cgcgAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcagcag                                          <  2:2032070/150‑1 (MQ=255)

                                                                                                             gcgAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAgg                                         >  2:296445/1‑150 (MQ=255)

                                                                                                               gAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCt                                       >  1:784133/1‑150 (MQ=255)

                                                                                                                        ctgctgGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGc                              <  2:6998982/150‑1 (MQ=255)

                                                                                                                          gctgGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCa                            >  2:430961/1‑150 (MQ=255)

                                                                                                                             ggACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGc                         <  1:7046205/150‑1 (MQ=255)

                                                                                                                              gACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCATCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCg                        >  2:4060148/1‑150 (MQ=255)

                                                                                                                                cTTCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGt                      >  2:5389810/1‑150 (MQ=38)

                                                                                                                                 ttCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGtt                     >  2:3747496/1‑150 (MQ=38)

                                                                                                                                 ttCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGtt                     >  2:2252694/1‑150 (MQ=38)

                                                                                                                                 ttCGGGGATGGCGTGGCGCAACACGCCTTCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGc                                                                                        >  1:4278472/1‑83 (MQ=21)

                                                                                                                                 ttCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGtt                     >  1:536730/1‑150 (MQ=255)

                                                                                                                                 ttCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGtt                     >  1:6776284/1‑150 (MQ=255)

                                                                                                                                  tCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTa                    >  2:1388997/1‑150 (MQ=255)

                                                                                                                                   cGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAc                   <  2:4225695/150‑1 (MQ=255)

                                                                                                                                   cGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAc                   <  2:150314/150‑1 (MQ=255)

                                                                                                                                    ggggATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGt                                <  1:5757806/134‑1 (MQ=38)

                                                                                                                                    gAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAcc                  >  2:6407567/1‑150 (MQ=255)

                                                                                                                                      ggAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGa                >  1:3476125/1‑150 (MQ=255)

                                                                                                                                       gAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGaa               <  2:571451/150‑1 (MQ=255)

                                                                                                                                       gAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGaa               >  1:4704751/1‑150 (MQ=255)

                                                                                                                                         aGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGa             <  1:836055/150‑1 (MQ=255)

                                                                                                                                            cGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCa          <  2:3941212/150‑1 (MQ=255)

                                                                                                                                             gtgtTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCAg         <  1:4759692/150‑1 (MQ=255)

                                                                                                                                              tgtTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCAGc        <  1:7177737/150‑1 (MQ=255)

                                                                                                                                                  gcgcCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCAGCAAGt    >  1:1056896/1‑150 (MQ=255)

                                                                                                                                                    gccacgccaTCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCAGCAAGTcc  >  2:3468415/1‑150 (MQ=255)

CTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCAGCAAGTCC  >  NC_002947/5192832‑5193129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`