Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,262,701 G→T 5.7% P33H (CCC→CAC)  PP_4634 ← alpha/beta family hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,262,7010GT 5.7% 1028.4 / 36.7 300P33H (CCC→CAC) PP_4634alpha/beta family hydrolase
Reads supporting (aligned to +/- strand):  ref base G (120/163);  new base T (4/13);  total (124/176)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 316 total aligned reads displayed.

CACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCT  >  NC_002947/5262552‑5262848
                                                                                                                                                     |                                                                                                                                                   
 aCGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTgggg                                                                                                                                                    >  1:1036777/1‑150 (MQ=255)
   gCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCCCCAGCGCCCGCTGGTGAt                                                                                                                                                  <  2:5567150/150‑1 (MQ=255)
   gCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGAt                                                                                                                                                  >  2:3725343/1‑150 (MQ=255)
   gCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGAt                                                                                                                                                  >  2:620179/1‑150 (MQ=255)
      gCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCCCCAGCGCCCGCTGGTGATCat                                                                                                                                               <  1:7219187/150‑2 (MQ=255)
       cGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAgg                                                                                                                                              >  1:3385177/1‑150 (MQ=255)
         aaGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCCCCAGCGCCCGCTGGTGATCatcaa                                                                                                                                            <  2:6366347/150‑5 (MQ=255)
          aGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTg                                                                                                                                           <  1:4887214/150‑1 (MQ=255)
          aGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTg                                                                                                                                           >  1:383041/1‑150 (MQ=255)
          aGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTg                                                                                                                                           <  2:3869792/150‑1 (MQ=255)
           gccggccgGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTgg                                                                                                                                          <  1:3709284/150‑1 (MQ=255)
            ccggccggGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTggg                                                                                                                                         <  1:4391578/150‑1 (MQ=255)
                 cggGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTgg                                                                                                                                    >  2:5290819/1‑150 (MQ=255)
                   ggATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGgcg                                                                                                                                  >  1:5580901/1‑150 (MQ=255)
                    gATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGgcgc                                                                                                                                 <  2:343470/150‑1 (MQ=255)
                    gATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGgcgc                                                                                                                                 <  1:3610999/150‑1 (MQ=255)
                       tCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcag                                                                                                                              <  1:5428396/150‑1 (MQ=255)
                         gCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcagca                                                                                                                            <  1:5033426/150‑1 (MQ=255)
                            gATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGaa                                                                                                                         <  2:2829735/150‑1 (MQ=255)
                             aTGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAg                                                                                                                        >  2:3325566/1‑150 (MQ=255)
                                  aCGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAgccgcc                                                                                                                   >  1:3293538/1‑150 (MQ=255)
                                  aCGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAgccgcc                                                                                                                   >  1:165801/1‑150 (MQ=255)
                                  aCGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAgccgcc                                                                                                                   >  1:772352/1‑150 (MQ=255)
                                      tAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAgg                                                                                                               <  1:7368915/150‑1 (MQ=255)
                                      tAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAgg                                                                                                               <  1:1833781/150‑1 (MQ=255)
                                        gTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGAAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCt                                                                                                             <  2:786058/150‑1 (MQ=255)
                                        gTCGGAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCACCGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTGGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCt                                                                                                             <  2:5142014/150‑1 (MQ=255)
                                         tCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTg                                                                                                            >  1:6552409/1‑150 (MQ=255)
                                         tCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTg                                                                                                            <  1:5663400/150‑1 (MQ=255)
                                           gTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTa                                                                                                          >  2:4795100/1‑150 (MQ=255)
                                            tAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTAt                                                                                                         >  2:263687/1‑150 (MQ=255)
                                            tAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTAt                                                                                                         >  2:4935674/1‑150 (MQ=255)
                                                tCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGt                                                                                                     >  2:6491274/1‑150 (MQ=255)
                                                tCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGt                                                                                                     >  2:2321012/1‑150 (MQ=255)
                                                 cAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTc                                                                                                    >  2:2920411/1‑150 (MQ=255)
                                                  aGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCg                                                                                                   <  1:5172656/150‑1 (MQ=255)
                                                  aGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCg                                                                                                   <  1:1403161/150‑1 (MQ=255)
                                                  aGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCg                                                                                                   >  1:7081318/1‑150 (MQ=255)
                                                   gCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCgg                                                                                                  >  1:4837598/1‑150 (MQ=255)
                                                   gCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCgg                                                                                                  >  2:4224752/1‑150 (MQ=255)
                                                    cacaTCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAgg                                                                                                               <  2:1811838/136‑1 (MQ=255)
                                                    cacaTCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGc                                                                                                 >  2:7372531/1‑150 (MQ=255)
                                                    cacaTCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGc                                                                                                 >  1:7215831/1‑150 (MQ=255)
                                                        tCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCcgcg                                                                                             <  2:1423853/150‑1 (MQ=255)
                                                            aGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGt                                                                                         <  2:4537193/150‑1 (MQ=255)
                                                               cGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCATCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGc                                                                                      <  2:5528390/150‑1 (MQ=255)
                                                               cGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGc                                                                                      <  1:664122/150‑1 (MQ=255)
                                                               cGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGc                                                                                      >  2:4596001/1‑150 (MQ=255)
                                                                      gAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGATGATGATCACCAGCGGCCGCTGGGGATCAGGTTGGGCGTGTCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATgg                                                                               >  2:4017080/1‑150 (MQ=255)
                                                                       aaCAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATggg                                                                              <  2:2033684/150‑1 (MQ=255)
                                                                            gTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGc                                                                         >  2:2411660/1‑150 (MQ=255)
                                                                            gTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGc                                                                         >  1:4983965/1‑150 (MQ=255)
                                                                            gTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGc                                                                         <  2:6268310/150‑1 (MQ=255)
                                                                             tAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCg                                                                        >  2:2516251/1‑150 (MQ=255)
                                                                             tAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCg                                                                        >  1:20798/1‑150 (MQ=255)
                                                                             tAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCg                                                                        >  1:2128511/1‑150 (MQ=255)
                                                                              aGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCgg                                                                       <  2:3056061/150‑1 (MQ=255)
                                                                                 cGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGtt                                                                    <  2:7170496/150‑1 (MQ=255)
                                                                                 cGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGtt                                                                    <  2:5293313/150‑1 (MQ=255)
                                                                                 cGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGtt                                                                    <  1:6000719/150‑1 (MQ=255)
                                                                                  ggCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTg                                                                   >  1:3535991/1‑150 (MQ=255)
                                                                                   gCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTgg                                                                  >  2:3021709/1‑150 (MQ=255)
                                                                                   gCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTgg                                                                  >  1:1310942/1‑150 (MQ=255)
                                                                                   gCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCGGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTgg                                                                  >  1:1161203/1‑150 (MQ=255)
                                                                                    cAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGa                                                                 <  1:960938/150‑1 (MQ=255)
                                                                                     aaaGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGAt                                                                <  1:3075333/150‑1 (MQ=255)
                                                                                     aaaGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGAt                                                                <  1:6815149/150‑1 (MQ=255)
                                                                                       aGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGa                                                              <  2:3220716/150‑1 (MQ=255)
                                                                                         cGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAgg                                                            <  1:266659/150‑1 (MQ=255)
                                                                                              gTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAgg                                                            <  1:4704090/145‑1 (MQ=255)
                                                                                                 aTAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCAt                                                    <  1:22393/150‑1 (MQ=255)
                                                                                                  tAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATg                                                   <  1:1208957/150‑1 (MQ=255)
                                                                                                  tAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATg                                                   <  1:4366266/150‑1 (MQ=255)
                                                                                                      ggCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGgcgc                                               >  2:1116879/1‑150 (MQ=255)
                                                                                                        cAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCAt                                             <  2:5029646/150‑1 (MQ=255)
                                                                                                           cGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTc                                          <  2:6532508/150‑1 (MQ=255)
                                                                                                             tACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCt                                        <  2:1729117/150‑1 (MQ=255)
                                                                                                             tACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCt                                        <  1:4796091/150‑1 (MQ=255)
                                                                                                              aCCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtg                                       <  1:3661440/150‑1 (MQ=255)
                                                                                                                cGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgtt                                     >  2:4329957/1‑150 (MQ=255)
                                                                                                                cGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgtt                                     >  1:4115320/1‑150 (MQ=255)
                                                                                                                 gAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttg                                    <  2:4694507/150‑1 (MQ=255)
                                                                                                                 gAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttg                                    >  2:5906412/1‑150 (MQ=255)
                                                                                                                  aaGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttgt                                   <  1:4486279/150‑1 (MQ=255)
                                                                                                                  aaGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttgt                                   >  2:6785095/1‑150 (MQ=255)
                                                                                                                   aGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttgtt                                  <  2:3812215/150‑1 (MQ=255)
                                                                                                                     tGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttgttgt                                <  1:2495450/150‑1 (MQ=255)
                                                                                                                      ggCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTtgtg                               >  2:1867398/1‑150 (MQ=255)
                                                                                                                       gCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTgc                              <  2:2157218/150‑1 (MQ=255)
                                                                                                                               gatgatCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAAc                      >  1:2980459/1‑150 (MQ=255)
                                                                                                                                atgatCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACt                     <  2:165801/150‑1 (MQ=255)
                                                                                                                                 tgatCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTa                    <  2:5221198/150‑1 (MQ=255)
                                                                                                                                  gatCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAg                   >  2:3579966/1‑150 (MQ=255)
                                                                                                                                  gatCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAg                   >  2:6158163/1‑150 (MQ=255)
                                                                                                                                     cACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCaa                <  1:620179/150‑1 (MQ=255)
                                                                                                                                      aCCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCaaa               >  1:3931891/1‑150 (MQ=255)
                                                                                                                                      aCCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCaaa               <  1:1462484/150‑1 (MQ=255)
                                                                                                                                       ccAGCGCCCGCTGGTGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAg              >  1:2842045/1‑150 (MQ=255)
                                                                                                                                        cAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGa             <  1:762684/150‑1 (MQ=255)
                                                                                                                                                   ggggATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCt  <  1:618026/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCT  >  NC_002947/5262552‑5262848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑