BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,423,815	G→T	5.7%	P85T (CCT→ACT)	PP_4758 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,423,815	0	G	T	5.7%	1072.0 / 38.3	314	P85T (CCT→ACT)	PP_4758	MFS transporter
Reads supporting (aligned to +/- strand): ref base G (139/157); new base T (13/5); total (152/162)
Fisher's exact test for biased strand distribution p-value = 5.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 316 total aligned reads displayed.

TGCTTCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACA  >  NC_002947/5423666‑5423964

 gCTTCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGt                                                                                                                                                      <  1:4056013/150‑1 (MQ=255)

  cTTCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTa                                                                                                                                                     <  2:2862404/150‑1 (MQ=255)

      ggCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTc                                                                                                                                                 >  2:1805057/1‑150 (MQ=255)

      ggCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTc                                                                                                                                                 <  2:7241189/150‑1 (MQ=255)

        ccACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGa                                                                                                                                               <  1:6378275/150‑1 (MQ=255)

        ccACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGa                                                                                                                                               <  1:3442386/150‑1 (MQ=255)

          aCCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAAc                                                                                                                                             >  2:371396/1‑150 (MQ=255)

           ccccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACa                                                                                                                                            <  2:831612/150‑1 (MQ=255)

            cccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAg                                                                                                                                           >  1:4773733/1‑150 (MQ=255)

             ccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgc                                                                                                                                          <  1:2063700/150‑1 (MQ=255)

              cGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgcg                                                                                                                                         >  1:4864184/1‑150 (MQ=255)

               gAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgcgc                                                                                                                                        <  2:5872457/150‑1 (MQ=255)

                    gAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAAc                                                                                                                                   <  2:2109805/150‑1 (MQ=255)

                     aaaaCGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAAcc                                                                                                                                  <  2:5329707/150‑1 (MQ=255)

                     aaaaCGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAAcc                                                                                                                                  >  2:4139452/1‑150 (MQ=255)

                       aaCGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAG‑CCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGAt                                                                                                                               <  2:4949602/150‑1 (MQ=255)

                           cagcaCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATgaa                                                                                                                            <  2:5118890/150‑1 (MQ=255)

                              caCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATgaagaa                                                                                                                         <  2:6450112/150‑1 (MQ=255)

                                     aaCTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCg                                                                                                                  <  1:148572/150‑1 (MQ=255)

                                     aaCTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCg                                                                                                                  <  1:3715013/150‑1 (MQ=255)

                                     aaCTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCg                                                                                                                  <  1:4922177/150‑1 (MQ=255)

                                      aCTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCgg                                                                                                                 >  2:5933042/1‑150 (MQ=255)

                                            tttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCAc                                                                                                              >  1:2183426/1‑147 (MQ=255)

                                             ttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCTGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGaa                                                                                                          <  2:781137/150‑1 (MQ=255)

                                             ttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGaa                                                                                                          <  1:2389206/150‑1 (MQ=255)

                                                 ttCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCa                                                                                                      >  1:363318/1‑150 (MQ=255)

                                                   cTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGCTGAAGAACAGCCCGGCACCGAAGCCATa                                                                                                    <  2:6677308/150‑1 (MQ=255)

                                                   cTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATa                                                                                                    <  1:5055456/150‑1 (MQ=255)

                                                   cTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATa                                                                                                    >  1:1223430/1‑150 (MQ=255)

                                                    tGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAg                                                                                                   <  2:6322985/150‑1 (MQ=255)

                                                    tGCACAAAGGCCAGGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCAtcg                                                                                                   <  2:6926241/150‑3 (MQ=255)

                                                     gCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGTACAGCCCGGCACCGAAGCCATAgg                                                                                                  >  2:4291923/1‑150 (MQ=255)

                                                      cacaAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAACCATAGGc                                                                                                 >  1:6992084/1‑150 (MQ=255)

                                                       acaAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGcc                                                                                                >  1:2530238/1‑150 (MQ=255)

                                                         aaaGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGc                                                                                              >  2:3287637/1‑150 (MQ=255)

                                                          aaGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCa                                                                                             <  1:6678818/150‑1 (MQ=255)

                                                            ggCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGa                                                                                                                                <  2:3047438/113‑1 (MQ=255)

                                                             gCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAgcg                                                                                          >  1:108061/1‑150 (MQ=255)

                                                              ccATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAgcgc                                                                                         >  1:3071071/1‑150 (MQ=255)

                                                               cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCt                                                                                        <  2:3851475/150‑1 (MQ=255)

                                                                   gCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATg                                                                                    <  1:3404126/150‑1 (MQ=255)

                                                                        ggCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAg                                                                               <  2:6573146/150‑1 (MQ=255)

                                                                          ccaccaGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGt                                                                             <  1:4622991/150‑1 (MQ=255)

                                                                           caccaGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTc                                                                            <  1:3210543/150‑1 (MQ=255)

                                                                             ccaGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGc                                                                          >  1:2970081/1‑150 (MQ=255)

                                                                              caGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCt                                                                         >  1:5407462/1‑150 (MQ=255)

                                                                                   cccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCa                                                                    >  1:997193/1‑150 (MQ=255)

                                                                                   cccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCa                                                                    <  1:1240233/150‑1 (MQ=255)

                                                                                   cccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCATTCCa                                                                    >  2:6809294/1‑150 (MQ=255)

                                                                                    ccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAg                                                                   <  2:4826066/150‑1 (MQ=255)

                                                                                    ccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAg                                                                   >  2:6542645/1‑150 (MQ=255)

                                                                                    ccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAg                                                                   >  1:6920843/1‑150 (MQ=255)

                                                                                     cAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAgg                                                                  >  2:3485715/1‑150 (MQ=255)

                                                                                         tGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcg                                                              <  1:6621870/150‑1 (MQ=255)

                                                                                             cATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgggcg                                                          >  2:4406862/1‑150 (MQ=255)

                                                                                             cATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgggcg                                                          <  1:6108045/150‑1 (MQ=255)

                                                                                             cATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgggcg                                                          >  2:171531/1‑150 (MQ=255)

                                                                                             cATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgggcg                                                          <  1:1587836/150‑1 (MQ=255)

                                                                                              aTGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTCCCAGGTgcgggcgg                                                         >  1:2730998/1‑150 (MQ=255)

                                                                                                gATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGAc                                                       <  1:1144336/150‑1 (MQ=255)

                                                                                                 aTACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACg                                                      <  1:4522949/150‑1 (MQ=255)

                                                                                                   aCGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGaa                                                    <  2:1668576/150‑1 (MQ=255)

                                                                                                   aCGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGaa                                                    >  1:185767/1‑150 (MQ=255)

                                                                                                         cAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAAt                                              <  2:5058973/150‑1 (MQ=255)

                                                                                                            ccACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGtt                                           <  2:2384309/150‑1 (MQ=255)

                                                                                                            ccACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGtt                                           <  1:711198/150‑1 (MQ=255)

                                                                                                              acaGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGa                                         >  2:493667/1‑150 (MQ=255)

                                                                                                                 gCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGAcgc                                      <  2:4752665/150‑1 (MQ=255)

                                                                                                                    gggCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGt                                   >  2:4884833/1‑150 (MQ=255)

                                                                                                                       cACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGa                                >  2:2949783/1‑150 (MQ=255)

                                                                                                                       cACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGa                                <  2:1402061/150‑1 (MQ=255)

                                                                                                                        aCCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAg                               <  1:6026862/150‑1 (MQ=255)

                                                                                                                        aCCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAg                               >  1:5758013/1‑150 (MQ=255)

                                                                                                                          cGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCa                                                <  2:3340339/131‑1 (MQ=255)

                                                                                                                          cGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGt                             >  1:7204377/1‑150 (MQ=255)

                                                                                                                          cGACCTATAGCAGCAGCATGATCGAAGGTACATCCAACATCGCGTAACCGATGAAGAACAGCCCGGCAACGAAGACATAGGCCGCAGCCCACATGCCCAGGACGCATTCCAGGTGCGGGCGGACGAAGACAAAGTTGACGATCTCGAGGt                             >  1:379264/1‑150 (MQ=255)

                                                                                                                            aCCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAg                           <  1:1716051/150‑1 (MQ=255)

                                                                                                                            aCCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAg                           <  2:7157241/150‑1 (MQ=255)

                                                                                                                             ccTTTTTCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGt                          <  2:1271244/150‑1 (MQ=255)

                                                                                                                               ttttGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTg                        >  2:4537907/1‑150 (MQ=255)

                                                                                                                               ttttGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTg                        >  2:200824/1‑150 (MQ=255)

                                                                                                                                 ttGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGAc                      <  2:722603/150‑1 (MQ=255)

                                                                                                                                   gcagcagcaTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGa                    <  2:3569073/150‑1 (MQ=255)

                                                                                                                                   gcagcagcaTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGa                    >  1:5099730/1‑150 (MQ=255)

                                                                                                                                          gctGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACat             >  2:3966479/3‑150 (MQ=255)

                                                                                                                                          gctGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACat             >  2:553362/3‑150 (MQ=255)

                                                                                                                                          gctGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACat             >  1:3874296/3‑150 (MQ=255)

                                                                                                                                            tGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga           >  1:6561350/1‑150 (MQ=255)

                                                                                                                                            tGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga           >  2:313968/1‑150 (MQ=255)

                                                                                                                                            tGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCCTAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga           >  2:2722873/1‑150 (MQ=255)

                                                                                                                                            tGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga           <  1:6487079/150‑1 (MQ=255)

                                                                                                                                             gTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatgat          >  2:2230882/1‑150 (MQ=255)

                                                                                                                                               tCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACAtgatga        <  1:4431387/150‑1 (MQ=255)

                                                                                                                                               tCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACAtgatga        <  1:4254593/150‑1 (MQ=255)

                                                                                                                                                cGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGAc       <  1:4638002/150‑1 (MQ=255)

                                                                                                                                                 gAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACg      >  1:5242143/1‑150 (MQ=255)

                                                                                                                                                  aaGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGa     >  2:2600711/1‑150 (MQ=255)

                                                                                                                                                  aaGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGa     >  2:424193/1‑150 (MQ=255)

                                                                                                                                                   aGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGaa    >  1:7314071/1‑150 (MQ=255)

                                                                                                                                                     gTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACa  >  1:1341385/1‑150 (MQ=255)

TGCTTCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACA  >  NC_002947/5423666‑5423964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`