BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,423,816	T→A	5.7%	V84V (GTA→GTT) ‡	PP_4758 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,423,816	0	T	A	5.7%	1081.5 / 36.8	314	V84V (GTA→GTT) ‡	PP_4758	MFS transporter
Reads supporting (aligned to +/- strand): ref base T (140/156); new base A (13/5); total (153/161)
Fisher's exact test for biased strand distribution p-value = 5.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 316 total aligned reads displayed.

GCTTCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAG  >  NC_002947/5423667‑5423965

gCTTCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGt                                                                                                                                                       <  1:4056013/150‑1 (MQ=255)

     ggCCACCCCGAGCAGAAAACGCAGCTCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTc                                                                                                                                                  <  2:7134076/150‑1 (MQ=255)

     ggCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTc                                                                                                                                                  <  2:7241189/150‑1 (MQ=255)

       ccACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGa                                                                                                                                                <  1:6378275/150‑1 (MQ=255)

       ccACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGa                                                                                                                                                <  1:3442386/150‑1 (MQ=255)

       ccACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTGTTGCAGCAGCATGTTCGAAGGTACTTCGa                                                                                                                                                >  2:3336430/1‑150 (MQ=255)

         aCCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAAc                                                                                                                                              <  2:2294450/150‑1 (MQ=255)

          ccccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAACa                                                                                                                                             <  2:6412306/150‑1 (MQ=255)

          ccccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACa                                                                                                                                             <  2:831612/150‑1 (MQ=255)

           cccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAg                                                                                                                                            >  2:3558843/1‑150 (MQ=255)

           cccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAg                                                                                                                                            >  1:4773733/1‑150 (MQ=255)

            ccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgc                                                                                                                                           >  2:5901830/1‑150 (MQ=255)

            ccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgc                                                                                                                                           <  1:2063700/150‑1 (MQ=255)

             cGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgcg                                                                                                                                          >  2:4279334/1‑150 (MQ=255)

             cGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgcg                                                                                                                                          >  1:4864184/1‑150 (MQ=255)

              gAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgcgc                                                                                                                                         <  2:4663150/150‑1 (MQ=255)

                    aaaaCGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAAcc                                                                                                                                   >  2:6648669/1‑150 (MQ=255)

                          cagcaCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATgaa                                                                                                                             <  2:2816270/150‑1 (MQ=255)

                                   gAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGccc                                                                                                                    <  2:5867570/150‑1 (MQ=255)

                                    aaCTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCg                                                                                                                   <  1:3715013/150‑1 (MQ=255)

                                    aaCTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCg                                                                                                                   <  2:3430441/150‑1 (MQ=255)

                                    aaCTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCg                                                                                                                   <  2:1872761/150‑1 (MQ=255)

                                    aaCTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCg                                                                                                                   <  1:4922177/150‑1 (MQ=255)

                                    aaCTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCg                                                                                                                   <  1:148572/150‑1 (MQ=255)

                                           tttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCAc                                                                                                               >  1:2183426/1‑147 (MQ=255)

                                            ttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCTGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGaa                                                                                                           <  2:781137/150‑1 (MQ=255)

                                            ttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGaa                                                                                                           <  1:2389206/150‑1 (MQ=255)

                                            ttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGaa                                                                                                           <  2:3693306/150‑1 (MQ=255)

                                              cGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGc                                                                                                         <  2:5051483/150‑1 (MQ=255)

                                                ttCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGt                                                                                                                                       <  2:4811915/118‑1 (MQ=255)

                                                ttCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCa                                                                                                       >  2:2945224/1‑150 (MQ=255)

                                                ttCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCa                                                                                                       >  1:363318/1‑150 (MQ=255)

                                                 tCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCAt                                                                                                      <  2:4850372/150‑1 (MQ=255)

                                                  cTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATa                                                                                                     >  2:4948200/1‑150 (MQ=255)

                                                  cTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATa                                                                                                     <  1:5055456/150‑1 (MQ=255)

                                                  cTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATa                                                                                                     >  1:1223430/1‑150 (MQ=255)

                                                   tGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAg                                                                                                    <  2:6533625/150‑1 (MQ=255)

                                                    gCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAgg                                                                                                   <  2:4923531/150‑1 (MQ=255)

                                                     cacaAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAACCATAGGc                                                                                                  >  1:6992084/1‑150 (MQ=255)

                                                      acaAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGcc                                                                                                 >  1:2530238/1‑150 (MQ=255)

                                                        aaaGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGc                                                                                               >  2:1804827/1‑150 (MQ=255)

                                                         aaGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCa                                                                                              <  1:6678818/150‑1 (MQ=255)

                                                           ggCCATGGCCGCGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGa                                                                                                                                 <  2:3047428/113‑1 (MQ=255)

                                                            gCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAgcg                                                                                           >  1:108061/1‑150 (MQ=255)

                                                             ccATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAgcgc                                                                                          >  1:3071071/1‑150 (MQ=255)

                                                               aTGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTg                                                                                        >  2:2537792/1‑150 (MQ=255)

                                                                 ggCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGAt                                                                                      <  2:6913427/150‑1 (MQ=255)

                                                                  gCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATg                                                                                     <  1:3404126/150‑1 (MQ=255)

                                                                         ccaccaGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGt                                                                              <  1:4622991/150‑1 (MQ=255)

                                                                          caccaGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTc                                                                             <  1:3210543/150‑1 (MQ=255)

                                                                          caccaGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTc                                                                             >  2:2046052/1‑150 (MQ=255)

                                                                            ccaGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGc                                                                           >  1:2970081/1‑150 (MQ=255)

                                                                             caGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCt                                                                          >  1:5407462/1‑150 (MQ=255)

                                                                                cccccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTc                                                                       <  2:4527485/150‑1 (MQ=255)

                                                                                  cccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCa                                                                     >  1:997193/1‑150 (MQ=255)

                                                                                  cccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCa                                                                     <  1:1240233/150‑1 (MQ=255)

                                                                                   ccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAg                                                                    >  1:6920843/1‑150 (MQ=255)

                                                                                     aGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGt                                                                  >  2:6318221/1‑150 (MQ=255)

                                                                                      ggTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTg                                                                 <  2:2226487/150‑1 (MQ=255)

                                                                                        tGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcg                                                               <  1:6621870/150‑1 (MQ=255)

                                                                                            cATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgggcg                                                           >  2:171531/1‑150 (MQ=255)

                                                                                            cATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgggcg                                                           <  1:1587836/150‑1 (MQ=255)

                                                                                            cATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgggcg                                                           >  2:4406861/1‑150 (MQ=255)

                                                                                            cATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgggcg                                                           <  1:6108045/150‑1 (MQ=255)

                                                                                             aTGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTCCCAGGTgcgggcgg                                                          >  1:2730998/1‑150 (MQ=255)

                                                                                               gATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGAc                                                        <  1:1144336/150‑1 (MQ=255)

                                                                                                aTACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACg                                                       <  1:4522949/150‑1 (MQ=255)

                                                                                                  aCGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGaa                                                     <  2:1668576/150‑1 (MQ=255)

                                                                                                  aCGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGaa                                                     >  1:185767/1‑150 (MQ=255)

                                                                                                       tCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCaa                                                >  2:5118459/1‑150 (MQ=255)

                                                                                                           ccACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGtt                                            >  2:3909745/1‑150 (MQ=255)

                                                                                                           ccACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGtt                                            <  1:711198/150‑1 (MQ=255)

                                                                                                              caGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGAc                                         <  2:5268832/150‑1 (MQ=255)

                                                                                                                   gggCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGt                                    >  2:3801995/1‑150 (MQ=255)

                                                                                                                      cACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGa                                 <  2:1402061/150‑1 (MQ=255)

                                                                                                                      cACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGa                                 >  2:4100823/1‑150 (MQ=255)

                                                                                                                       aCCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAg                                <  2:5357003/150‑1 (MQ=255)

                                                                                                                       aCCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAg                                <  1:6026862/150‑1 (MQ=255)

                                                                                                                       aCCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAg                                >  1:5758013/1‑150 (MQ=255)

                                                                                                                         cGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGt                              >  1:7204377/1‑150 (MQ=255)

                                                                                                                         cGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGt                              >  2:4216818/1‑150 (MQ=255)

                                                                                                                         cGACCTATAGCAGCAGCATGATCGAAGGTACATCCAACATCGCGTAACCGATGAAGAACAGCCCGGCAACGAAGACATAGGCCGCAGCCCACATGCCCAGGACGCATTCCAGGTGCGGGCGGACGAAGACAAAGTTGACGATCTCGAGGt                              >  1:379264/1‑150 (MQ=255)

                                                                                                                           aCCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAg                            <  2:6554276/150‑1 (MQ=255)

                                                                                                                           aCCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAg                            <  2:7211456/150‑1 (MQ=255)

                                                                                                                           aCCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAg                            <  1:1716051/150‑1 (MQ=255)

                                                                                                                            ccTTTTTCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGt                           <  2:1271244/150‑1 (MQ=255)

                                                                                                                              ttttGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTg                         >  2:3209228/1‑150 (MQ=255)

                                                                                                                              ttttGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTg                         >  2:6750741/1‑150 (MQ=255)

                                                                                                                                  gcagcagcaTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGa                     >  1:5099730/1‑150 (MQ=255)

                                                                                                                                     gcagcaTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGa                  <  2:2671368/150‑1 (MQ=255)

                                                                                                                                         gctGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACat              >  1:3874296/3‑150 (MQ=255)

                                                                                                                                           tGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga            >  2:313839/1‑150 (MQ=255)

                                                                                                                                           tGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga            >  1:6561350/1‑150 (MQ=255)

                                                                                                                                           tGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga            <  1:6487079/150‑1 (MQ=255)

                                                                                                                                              tCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACAtgatga         <  1:4254593/150‑1 (MQ=255)

                                                                                                                                              tCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACAtgatga         <  1:4431387/150‑1 (MQ=255)

                                                                                                                                               cGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGAc        <  1:4638002/150‑1 (MQ=255)

                                                                                                                                                gAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACg       >  1:5242143/1‑150 (MQ=255)

                                                                                                                                                  aGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGaa     >  1:7314071/1‑150 (MQ=255)

                                                                                                                                                    gTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACa   >  1:1341385/1‑150 (MQ=255)

GCTTCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAG  >  NC_002947/5423667‑5423965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`