Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,451,286 T→G 12.5% L145R (CTG→CGG)  thiE → thiamine‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,451,2860TG12.5% 595.3 / 11.2 292L145R (CTG→CGG) thiEthiamine‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (142/102);  new base G (27/18);  total (171/121)
Fisher's exact test for biased strand distribution p-value = 8.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

TCGGCCGTCAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCA  >  NC_002947/5451139‑5451435
                                                                                                                                                    |                                                                                                                                                     
  ggCCGTCAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGcgc                                                                                                                                                   >  1:3946468/1‑148 (MQ=255)
    ccGTCAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCCGCGAAGGCGCCCGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCgc                                                                                                                                                 >  2:7321305/1‑149 (MQ=255)
    ccGTCAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTTGCCGCTTCTTCTATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGa                                                                                                                                                 <  2:7367010/150‑1 (MQ=255)
    ccGTCAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGa                                                                                                                                                 >  2:5067386/1‑150 (MQ=255)
         aGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAgg                                                                                                                                            <  2:6463556/150‑1 (MQ=255)
          ggCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCGCGAGCCGGc                                                                                                                                           >  2:371704/1‑150 (MQ=255)
           gCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGTGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCGCGAGCAGGcc                                                                                                                                          >  1:2057055/1‑150 (MQ=255)
           gCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCGTTGGCCGCTTCTGCAAGTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGcc                                                                                                                                          >  2:5480637/1‑150 (MQ=255)
             aaTCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCGGCTCGAGCAGGCCcg                                                                                                                                        >  2:6558194/1‑150 (MQ=255)
               tcagcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCCGGccgacg                                                                                                                                      <  2:5226999/150‑5 (MQ=255)
                catcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCgcgc                                                                                                                                     >  1:1779044/1‑150 (MQ=255)
                  tcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCCCGccc                                                                                                                                   >  1:5643883/1‑150 (MQ=255)
                   gggCTCCACCTGCCCCGCCAGCCTCGTACTGGCCGCCCAGGCTGCCAGCGATGGCGCCTGCTTGGTGGCCTTTGGCGGCTCCTTAATTTCTGTCACCACGCGGGGCGCGCCG‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCa                                                                                                                                  <  1:3544276/149‑1 (MQ=255)
                   cGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTCCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCGGCTCGAGCAGGCCCGCGCCCa                                                                                                                                  >  2:3544276/1‑150 (MQ=255)
                    ggCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAg                                                                                                                                 <  2:4815580/150‑1 (MQ=255)
                     gCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCCGCTACGTGGCCTGTGGCCGCGTCTTCCATTCTGTCCCCCAGCCGGGCGCGCCC‑GCTGCCAACGGCGGCCGGCGCGCGCCCGCCCGCGcccccg                                                                                                                                >  2:4214461/1‑147 (MQ=255)
                     gCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAgg                                                                                                                                >  2:3247484/1‑150 (MQ=255)
                         cACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCCGGTGaa                                                                                                                            >  1:6267433/1‑150 (MQ=255)
                             tGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGGCTGCTCGCGCAGGCCCGCGCCCCCGGGAAACTg                                                                                                                        >  1:4571376/1‑150 (MQ=255)
                              accACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCAGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGc                                                                                                                       <  2:1792979/149‑1 (MQ=255)
                                   gCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATc                                                                                                                  <  2:7068324/150‑1 (MQ=255)
                                      aGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCCGGTGAAAATGCCGATCGcc                                                                                                               >  2:3417557/1‑150 (MQ=255)
                                       gCCTCGAACTGGCCGCCCAGGCTGCCAGCGACGGCGCCAGCTACGTGACCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCACCGTCGGCCTGCTCGAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCg                                                                                                              <  2:881560/150‑1 (MQ=255)
                                        ccTCGAACTGGTCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCGGCCACCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGt                                                                                                             <  2:4707855/150‑1 (MQ=255)
                                         cTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCCGGCCCCCGCCCAGGTGAAACAGCCCGGCGCCGTg                                                                                                            >  1:6099373/1‑150 (MQ=255)
                                         cTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTg                                                                                                            >  1:3075569/1‑150 (MQ=255)
                                          tCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGa                                                                                                           >  2:2355322/1‑150 (MQ=255)
                                          tCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCGCGAGCCGGCCCGCGCCCCGGGGAAACAGCCGATCGCCGGGa                                                                                                           >  1:3440800/1‑150 (MQ=255)
                                           ttcgcTGGCAGCCTGGGCGGCCAGTGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCCCGCCCAGGTGAAACTGCCGATCGCCCTGAt                                                                                                          >  2:4635114/5‑150 (MQ=255)
                                             aaCTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTGGAGCAGGCCCGCGCCCAGGTGGAAATGCCGATCGCCGTGAtag                                                                                                        >  1:2889923/1‑148 (MQ=255)
                                                 ggCCGCCCTGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCGGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGCGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTgg                                                                                                    <  2:1970443/150‑1 (MQ=255)
                                                 ggCCGCCCCGGCTGCCCGCGCCGGCGCCCGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGCCACCACGCGGGGCGCGCCC‑GCTGCCACCGGCGGCCGGCTCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTgg                                                                                                    <  2:1418884/150‑1 (MQ=255)
                                                  tcgaatcAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCCGGTGAAACTGCCGATCGCCGTGATCGGTGGc                                                                                                   >  2:3679572/7‑150 (MQ=255)
                                                  gccgccCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGc                                                                                                   >  2:3843190/1‑150 (MQ=255)
                                                     gccCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGGCGGCGCGAGCAGGCCCGCGCCCCCGGGACACTGCCCAGCGCCGGGAGCGGTGGcctg                                                                                                >  1:3181551/1‑147 (MQ=255)
                                                     gccCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTGCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCCGGTGAAACTGCCGATCGCCGTGCTCGGGGGCCTc                                                                                                >  2:4345264/1‑150 (MQ=255)
                                                       ccAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGCAACTGCCGATCGCCGGGATCGGTGGCATCAc                                                                                              >  2:2164413/1‑150 (MQ=255)
                                                       ccAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCAc                                                                                              <  2:1040672/150‑1 (MQ=255)
                                                           gCTGCCAGCGACGGCGCCAGCTACGTGGCCTTTGGCCGCTCCTTCAAGTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTc                                                                                          <  2:6952654/150‑1 (MQ=255)
                                                            cTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCGGCTCGAGCAGGCCCGCGCCCAGGGGAAACTGCCCCTCGCCGTGATCGGTGGCATCACCCACg                                                                                         >  1:5987132/1‑150 (MQ=255)
                                                             tGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGACACCACGCCGGGCGCGCCC‑GCCGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGa                                                                                        <  2:1306677/150‑1 (MQ=255)
                                                                 aGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCACGCCCGCGCCCAGGTGAAACAGCCGAGCGCCGTGATAGGTGGCATCACCCACCACAAc                                                                                    >  1:851940/1‑150 (MQ=255)
                                                                   cgtcggCGCCAGCTCGGTGGCCTTTGGCGGATTCTTCACTTGTGCCACAACGCGGGGCGCGCCC‑GCCCCCACCGTCGGCCTGCTCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAAcgc                                                                                  <  1:5201738/146‑1 (MQ=255)
                                                                        gcgcCCGCTAGGTGGCATTTTGCCGCTACTTCAATTCTGTCACCATGCCGGGCGCGCCC‑GCTGCCAAGGTCGGCCTGCTCTAGGCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGccc                                                                             <  2:6708781/150‑1 (MQ=255)
                                                                          gcCAGCTACGTGGCCTTTGGCCGCTTATTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCCCCCCCAGGTGAAACTGACGCTCTCCCTGCTCGGTGGCCTCACCCACGACAACGCCCCCCCa                                                                           >  2:6288599/1‑150 (MQ=255)
                                                                             aGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCCCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCACGACAACGCCGCCCCACTg                                                                        >  1:6677981/1‑150 (MQ=255)
                                                                                   ttGGACTTTGGCCTATTCTTCTATTCTGTCACCCCGCGGGGGGCGCCC‑GCTGCCACCGTCGGCCTGCTGGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGcc                                                                  <  2:99207/149‑1 (MQ=255)
                                                                                    tGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACAGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCCCTGGTCGccc                                                                 >  2:1458890/1‑150 (MQ=255)
                                                                                     ggCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACAGCCGATCGCCAACATCGGTGGCATAACCCACGACAACCCCCCCCCACAGGGCGCCCa                                                                >  1:7316145/1‑150 (MQ=255)
                                                                                       ccTTTGGCCTCTTCTTAAATTCTGTCACCACGCCGGGCGCGCCC‑GCGGCCACCGGCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTTGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACg                                                              <  1:4211165/150‑1 (MQ=255)
                                                                                        cTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATAGCCGTGATCGGTGGCATCACCCTCCACAACGCCGCCCCACTGGTCGCCCACgg                                                             >  2:2712613/1‑150 (MQ=255)
                                                                                        cTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCGGCTCGAGCAGGCCCGCCCCCACGTGAAAAAGCCGAAAGCCCTGATAGGTGGCATCACACACGACAAACCCCCCCCCCAGGTCGCCCAccc                                                             >  1:4542967/1‑148 (MQ=255)
                                                                                         tttGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGgc                                                            >  2:661818/1‑150 (MQ=255)
                                                                                              ccGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGa                                                       >  2:5293369/1‑150 (MQ=255)
                                                                                              ccGCTTCTACAAATCGGTCACCACCCCGGGCGCGCCC‑GCGGCCACCGCCGGCCTGCTCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGa                                                       <  1:2344504/150‑1 (MQ=255)
                                                                                               cGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGAc                                                      >  2:3895275/1‑150 (MQ=255)
                                                                                               cGCTTCTTCAATTCTGGCCCCCAGCCGGGCGCGCCC‑GCTGCCACCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGAc                                                      <  2:4157683/150‑1 (MQ=255)
                                                                                                 cttcttCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACAGGTCGCCCACGGCGCCGACct                                                    >  2:2448685/1‑150 (MQ=255)
                                                                                                  ttcttcAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCCGGCCCGCGCCCACGTGAAACTGCCGATCGCCGGGATCGGTGGCCTCACCCTCGACAACGCCCCCCCCCTCGTCGCCCACCGCGccccccgg                                                   >  1:4095463/1‑144 (MQ=255)
                                                                                                  ttcttcAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACctg                                                   >  2:6085739/1‑150 (MQ=255)
                                                                                                    cttcAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGcc                                                         >  2:6199560/1‑142 (MQ=255)
                                                                                                     ttcAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGCGCACGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCACGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACctgctg                                                >  2:253970/1‑150 (MQ=255)
                                                                                                     ttcAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCACGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTCGTCGCCCACGGCGCCGACctgctg                                                >  1:1333280/1‑150 (MQ=255)
                                                                                                     tacAATTCTCTCACCAACCCGGCCCCGCCC‑GCTCCCAACGCCGCCCTGCACCAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTTATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACctgctg                                                <  2:6221165/148‑1 (MQ=255)
                                                                                                        atttCTGTCACCCAGCGGGGCGCGCCC‑GCTGCCACCGTCGGCCTGCTCGAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCg                                             <  1:2220342/148‑1 (MQ=255)
                                                                                                        aaTACTGTCACCACGCCGGGCGCGCCC‑GCTGCAAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCg                                             <  2:7428421/150‑1 (MQ=255)
                                                                                                         aTTCTGCCACCCCGCCGGGCGCGCCC‑GCTGCCACCGGCGGCCTGCTCGCGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCgg                                            <  1:1105265/150‑1 (MQ=255)
                                                                                                            cTGTCCCCACGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCGGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGa                                         <  2:6876790/150‑1 (MQ=255)
                                                                                                            cTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCAACAATGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGa                                         >  2:2977197/1‑150 (MQ=255)
                                                                                                             tGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACAGCCGATCGCCGTGATCGGTGGCAACACCCACGACAACGCCGCCCCACAGGTCGCCCACGGCGCCGACCAGCTGGCGGTGAt                                        >  1:6147363/1‑150 (MQ=255)
                                                                                                                cccccccccGGGCGCGCCC‑GCCGCCCCGGGGGGCCTGCTCTCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCa                                     <  1:6909517/143‑1 (MQ=255)
                                                                                                                 cccACGCCGGGCGCGCCC‑GCGGCCCCCGGCGGCCGGCGCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCCCTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCAc                                    <  2:2880103/149‑1 (MQ=255)
                                                                                                                 aCCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGCGCAGGCCCGCGCCCAGGTGAAACAGCCGCGCGCCGGGATCGGTGGCATCACCCGCGACAACGCCGCCCCACTGGTCGCCCACCGCGCCGACCAGCTGGCGGTGATCCAc                                    >  1:153424/1‑150 (MQ=255)
                                                                                                                 aCCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCAc                                    >  2:3013486/1‑150 (MQ=255)
                                                                                                                 aCCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGGGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCAc                                    >  1:480340/1‑150 (MQ=255)
                                                                                                                 aCCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCGCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCCACAACCCCGCCCCACGGGTCGCCCACCGCCCCGACCAGCTGGCGGTGATCCAc                                    >  1:499204/1‑150 (MQ=255)
                                                                                                                  ccAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCGGCTCGAGCAGGCCCGCGCCCCGGTGAAACATCCGATCGCCGTGATCGGTGGCCTCACCCACGACAACGCCGCCCCACAGGTCGCCCACCGCCCCGACCAGCTGGCGGTGATCCAcc                                   >  1:5772119/1‑149 (MQ=255)
                                                                                                                   ggcagcGGGCGCGCCCGGCTGCCAACGTCGGCCGGCTCGAGCAGGCCCGCGCCCAGGGGAAACTGCCGATCGCCGTGATCGGTGGCCTCACCCGCGACAACGCCGCCCCACACGTCGCCCACCCCGCCCACCAGCAGGCGGTGATCCAcc                                   >  1:2557624/6‑149 (MQ=255)
                                                                                                                   cAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACgg                                  >  2:7270314/1‑150 (MQ=255)
                                                                                                                   cAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACgg                                  >  1:4309185/1‑150 (MQ=255)
                                                                                                                    cccccGGGCGCGCCC‑GGGGCCACCGGCGGCCGGCCCGCCGCCGCCCGCGCCCCCGTGAAACGGCCGATCGCCGTGATCGGTTGGCTCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGc                                 <  2:5609321/147‑1 (MQ=255)
                                                                                                                    aaGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCGCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCCCCCCACAGGTCGCCCACCGCGCCGCCCAGCTGGCGGTGATCCACGGc                                 >  1:6452035/1‑150 (MQ=255)
                                                                                                                     cgCCGGGCGCGCCC‑GCTGCCACCGGCGGCCTGCTCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCt                                <  2:4046008/149‑1 (MQ=255)
                                                                                                                         gggCGCGCCC‑GCTGCCCCCGTCGGCCGGCTCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGtt                            <  1:4647880/150‑1 (MQ=255)
                                                                                                                          ggCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTc                           >  1:19938/1‑150 (MQ=255)
                                                                                                                           gcgcgcCG‑GCGCCCCGCGGCGGCCGGCTCGCCCCGCCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCg                          <  1:3807929/150‑1 (MQ=255)
                                                                                                                           gcgcgcCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCg                          >  2:2424700/1‑150 (MQ=255)
                                                                                                                                  c‑GCTCCCAACGACCGCCTGCTCGACCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGa                   <  2:162445/150‑1 (MQ=255)
                                                                                                                                        ccAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcg              >  1:2991338/1‑150 (MQ=255)
                                                                                                                                        acAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCACGACAACGCCGCCCCACAGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcg              >  1:3688343/2‑150 (MQ=255)
                                                                                                                                          aaCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgcg            >  2:3739485/1‑150 (MQ=255)
                                                                                                                                          aaCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgcg            >  2:2036069/1‑150 (MQ=255)
                                                                                                                                          aaCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgcg            >  1:5874504/1‑150 (MQ=255)
                                                                                                                                          aaCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAAAGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgcg            >  1:1676365/1‑150 (MQ=255)
                                                                                                                                               cGGCCTGCGCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGa       <  2:5935459/150‑1 (MQ=255)
                                                                                                                                                ggCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGaa      <  1:5394402/150‑1 (MQ=255)
                                                                                                                                                ggCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGaa      >  1:3219161/1‑150 (MQ=255)
                                                                                                                                                   cTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTc   >  2:3562712/1‑150 (MQ=255)
                                                                                                                                                    tGCTCGAGCAGGTCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCa  >  1:6867119/1‑150 (MQ=255)
                                                                                                                                                    tGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCa  >  2:5703424/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCGGCCGTCAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCC‑GCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCA  >  NC_002947/5451139‑5451435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑