Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,657,963 A→T 12.8% intergenic (‑64/+97) PP_4961 ← / ← PP_4962 lipoprotein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,657,9630AT12.8% 660.4 / 62.2 265intergenic (‑64/+97)PP_4961/PP_4962lipoprotein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (120/94);  new base T (7/29);  total (129/136)
Fisher's exact test for biased strand distribution p-value = 4.87e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

TTGTCTGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTACTTTTCCAG  >  NC_002947/5657814‑5658110
                                                                                                                                                     |                                                                                                                                                   
ttGTCTGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACa                                                                                                                                                     <  1:6514435/150‑1 (MQ=255)
ttGTCTGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACa                                                                                                                                                     <  2:634900/150‑1 (MQ=255)
  gTCTGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGt                                                                                                                                                   <  2:3010461/150‑1 (MQ=255)
   tCTGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTg                                                                                                                                                  <  1:1476316/150‑1 (MQ=255)
     tGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAACAGCGCTTTCATTCAGTGTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCa                                                                                                                                                <  2:4180356/150‑1 (MQ=255)
     tGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTCCAGTGCa                                                                                                                                                >  2:5212571/1‑150 (MQ=255)
     tGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCa                                                                                                                                                <  2:6552533/150‑1 (MQ=255)
     tGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGGTTCCTCGATGTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCa                                                                                                                                                <  1:5807624/150‑1 (MQ=255)
       ccGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGt                                                                                                                                              <  1:3878241/150‑1 (MQ=255)
        cGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGtc                                                                                                                                             >  2:543887/1‑150 (MQ=255)
          gggCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGGAGtctg                                                                                                                                           >  1:4064424/1‑149 (MQ=255)
             ccGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCtgt                                                                                                                                        <  1:6261747/150‑1 (MQ=255)
             ccGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCtgt                                                                                                                                        <  2:2838693/150‑1 (MQ=255)
             ccGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCtgt                                                                                                                                        <  1:5270752/150‑1 (MQ=255)
               ggctGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTgg                                                                                                                                      <  2:755118/147‑1 (MQ=255)
               ggTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTgg                                                                                                                                      <  2:218700/150‑1 (MQ=255)
               ggTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTgg                                                                                                                                      <  2:6857686/150‑1 (MQ=255)
               ggTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTgg                                                                                                                                      <  2:2658180/150‑1 (MQ=255)
               ggTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCGCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTgg                                                                                                                                      <  2:3529961/150‑1 (MQ=255)
                 ttGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGa                                                                                                                                    <  1:1816710/150‑1 (MQ=255)
                  tGCGCCGGCTGCAGCTGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAg                                                                                                                                   <  2:7014726/150‑1 (MQ=255)
                  tGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCCAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGACTCTGTTGGAg                                                                                                                                   >  1:4549418/1‑150 (MQ=255)
                  tGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTGTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAg                                                                                                                                   <  2:4557407/150‑1 (MQ=255)
                   gcgcCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGAGACTGCACTGTACCTGTGGGAGc                                                                                                                                  <  1:1994465/150‑1 (MQ=25)
                     gcCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCgg                                                                                                                                <  1:611619/150‑1 (MQ=255)
                     gcCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGAGACTGCACTGTACCTGTGGGAGCgg                                                                                                                                <  1:2784811/150‑1 (MQ=25)
                         gCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGa                                                                                                                            <  2:904666/150‑1 (MQ=255)
                         gCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGa                                                                                                                            <  1:5658367/150‑1 (MQ=255)
                         gCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGa                                                                                                                            <  1:4304186/150‑1 (MQ=255)
                             cagcagTGAGCATCCTGCCAGTGTCGCCTGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGAGACTGCACTGTACCTGTGGGAGCGGGCGAGccc                                                                                                                        <  2:107550/150‑1 (MQ=21)
                                       cATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCa                                                                                                                                                <  2:2417040/116‑1 (MQ=255)
                                       cATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGcc                                                                                                              >  1:7287768/1‑150 (MQ=255)
                                          ccTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTGTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCGCGGGCTCGCCCGCTCCCACAGAGACTGCACTGTACCTGTGGGAGCGGGCGAGCCCGAGAAGGGCTGca                                                                                                           <  2:1205815/150‑1 (MQ=17)
                                            tGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGCAGGGCCGcaca                                                                                                         >  1:3237939/1‑150 (MQ=255)
                                            tGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGcaca                                                                                                         >  1:4692964/1‑150 (MQ=255)
                                            tGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGcaca                                                                                                         >  1:3237731/1‑150 (MQ=255)
                                                aGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCgg                                                                                                     >  1:5981099/1‑150 (MQ=255)
                                                 gtgtCGCCCGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTGTCCTCGATTTGGGGCTGCTGTGCAGCCGTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCCGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGc                                                                                                    <  2:2173554/150‑1 (MQ=255)
                                                 gggtCGCCTGGGCCGTCACGGCCAAACGCGCGTTACTTCTGGGCCCTCGATTGGGGGCTGCGGTGCAGCCCTTCTCGGGCCCGCCGGCCCCCACCGGTACCGTGCCGTCGCTGGGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGc                                                                                                    <  2:5179184/148‑1 (MQ=255)
                                                   gtCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGa                                                                                                                                    <  1:434418/116‑1 (MQ=255)
                                                   gtCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGa                                                                                                                                    >  2:434418/1‑116 (MQ=255)
                                                         tgggCCGTCACGGCCAAAAGCGCTTTCATCCAGTTTCCTCTATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCCCCGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCcttctt                                                                                            <  1:6044889/149‑1 (MQ=255)
                                                          gggCCGTCCCGGCCAACCGCGCTTACATACCGTGCCCTCGATTTGGGGCTGCTGGGCAGCCCTTCTGGGGCGCGCCCGCCCCCCCCGGTACCGGCCAGTCTCTGGGGTCGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCttcttc                                                                                           <  1:509509/150‑1 (MQ=255)
                                                              cGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCCGtct                                                                                                                                            >  1:4462596/1‑97 (MQ=255)
                                                              cGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGTGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGa                                                                                       <  2:3608029/150‑1 (MQ=255)
                                                                  aCGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACcac                                                                                   >  1:1785219/1‑150 (MQ=255)
                                                                    ggCCAAAAGCGCTTCCATTCAGTTCCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCCCCGGTACAGTGCAGTCTCTGGGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACaa                                                                                 <  1:1534624/150‑1 (MQ=255)
                                                                      ccAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAAt                                                                               >  2:6067119/1‑150 (MQ=255)
                                                                          atgcgcTTTCCTGCGGTGTCCGGGTTGTGGGGGTGGGGTGCCGCCGTGCGGGGGCTCGCCGGCGCCCCCTGTTACCGTGCTGTCGGGGGGGGTGGGGGGTCGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAAc                                                                           <  1:2132569/148‑1 (MQ=255)
                                                                          aaGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAAc                                                                           <  2:1191054/150‑1 (MQ=255)
                                                                               ctttatttcaTTTTCCTCGTTTGGGGGCGGCTGTGCAGCCATTCTGGGGCTCGCCCGCTCCCACCGGTACAGTGCAGTCTCTGTGGGAGGGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCa                                                                      <  1:1184140/144‑1 (MQ=255)
                                                                                   ctttcaGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCGCGGGCTCGCCCGCTCCCACCGGTACAGTGCTGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGtt                                                                  <  2:6316759/148‑1 (MQ=255)
                                                                                    attcaGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTATGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACACCGGCCCCGTCTTCAAGACCACAAAACAACGTTCAGGTTg                                                                 >  1:3474731/1‑150 (MQ=255)
                                                                                     tgccgTTTCCTCGTTTGGGGGCGGCTGTGCAGCCCTTCTCGGGCGCGCCCGCTCCCACCGGTACAGTGCAGTCTCGGTGGGAGGGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGc                                                                <  2:189139/146‑1 (MQ=255)
                                                                                     tgcaGTTTCCTCGATTTGGGGCGGCTGTGCAGCCCTTCTCGGGCTCGCCCGCGCCCACTGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGc                                                                <  2:3901347/148‑1 (MQ=255)
                                                                                       caGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCt                                                              >  2:6520699/1‑150 (MQ=255)
                                                                                        cgTTCCCTCTTTTGGGGGCTGCTGGGCCGCCCTTCTCGGGCCCGCCCCCCCCCACTGGTACTGTGCAGACGGTGGGGGAGGGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTa                                                             <  1:6987275/149‑1 (MQ=255)
                                                                                           ttCCTCGTTTGGGGGCTGGTGTGCCGCCCTTCTCGGGCTCGCCCGGTCCCCCCGGTACTGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTa                                                          <  1:2445381/150‑1 (MQ=255)
                                                                                             ccTCGATTGGGGGCTGGTGTGCCGCCCTTCGCGGGCTCGCCCGCTCCCACCGGTACTGTGCTGTCTCTGTGGGTGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTAcc                                                        <  2:2486786/150‑1 (MQ=25)
                                                                                              cTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTAccc                                                       >  1:3543165/1‑150 (MQ=255)
                                                                                              cTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCAGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTAccc                                                       >  2:5708089/1‑150 (MQ=255)
                                                                                                  aTTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCCCAAATCAACGTTCAGGTTGCCTATTACCCGATc                                                   >  2:3935287/1‑150 (MQ=255)
                                                                                                      ggggCTGCTGTGCAGCCCTTCTCGGGCTCGCCTGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAgg                                               >  2:4820891/1‑150 (MQ=255)
                                                                                                      ggggCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACTGTGCAGTCTCTGTGGTAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAgg                                               <  2:5038400/150‑1 (MQ=255)
                                                                                                      ggggCTGCTGTGCAGCCCTTCGCGGGCGCCCCCGCCCCCCCCGGTACCGTGCTGTCTGTGTGGGCGGGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAgg                                               <  1:822764/150‑1 (MQ=255)
                                                                                                       gggCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGTTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAggg                                              <  2:993574/150‑1 (MQ=255)
                                                                                                       gggCTGCACAGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACACTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCCCACAGCGGCCCCTTCTTCAAGAACACAAATCAACGTTCAGGTTGCCAATAACCAGATCAAtgg                                              >  1:3081884/1‑150 (MQ=37)
                                                                                                         gctgctGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCa                                            >  1:7408891/1‑150 (MQ=255)
                                                                                                         gctgctGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCa                                            >  2:1501237/1‑150 (MQ=255)
                                                                                                                  cAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGgcgc                                   <  2:5487642/150‑1 (MQ=255)
                                                                                                                   ggCCCTTCACGGGCTCGCCCGCTCCCACAGAGACTGCACTGTACCTGTGGGAGCGGGCGAGCCCGAGAAGGGCTGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGcc                                  >  2:2218977/2‑150 (MQ=18)
                                                                                                                     cccTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTAc                                                         <  2:2363166/125‑1 (MQ=255)
                                                                                                                      ccTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTg                               <  2:1115846/150‑1 (MQ=255)
                                                                                                                          ctcGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCt                           >  1:5469234/1‑150 (MQ=255)
                                                                                                                            cGGGCCCCCCCCCACCCACATGTAATTTGCCTTATCTGTGGCCGGGGGCTAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCtgg                         <  1:1287564/150‑1 (MQ=255)
                                                                                                                             gggCTCGCCCGCTCCCCCCGGTACCGGGCAGTCGCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACCACGCAACGTTCAGGTTGCCTATGACCCGATCAAGGGCAACCCGGCGCCGTGCGCtggt                        <  2:5296848/150‑1 (MQ=38)
                                                                                                                             gggCTCGCCCGCTCCCACCGGTACAGTGCCGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGCTCAAGGGCAACCCGGCGCCTTGCGCtggt                        <  2:376525/150‑1 (MQ=255)
                                                                                                                             gggCTCGCCCGCTCCCACAGGTACTGTGCTGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCtggt                        <  2:1418520/150‑1 (MQ=255)
                                                                                                                             gggCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCtggt                        >  2:325896/1‑150 (MQ=255)
                                                                                                                             gggCTCGCCCGCTCCCACAGAGACTGCACTGTACCTGTGGGAGCGGGCGAGCCCGAGAAGGGCTGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCtggt                        <  1:2218977/150‑1 (MQ=21)
                                                                                                                               gCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAg                                                                     >  1:227799/1‑103 (MQ=255)
                                                                                                                                 tCGCCCGCTCCCACAGAGACTGCACTGTACCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTc                    >  1:5317072/1‑150 (MQ=25)
                                                                                                                                  cGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCt                   >  1:495880/1‑150 (MQ=255)
                                                                                                                                   gCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCtt                  >  1:4764065/1‑150 (MQ=255)
                                                                                                                                    cccGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTtc                 >  2:3564735/1‑150 (MQ=255)
                                                                                                                                     ccGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCGGGTGGTCTtcg                >  2:2678749/1‑150 (MQ=255)
                                                                                                                                     ccGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGAACACAAATCAACGTTCAGGTTGCCTATTACACGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTtcg                >  1:559765/1‑150 (MQ=255)
                                                                                                                                       gCTCCCACTGTTACAGTGCAGTCTCTGTGGGCGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTtcgtc              <  1:3200589/150‑1 (MQ=255)
                                                                                                                                         tCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTcgtcgt            >  1:4947949/1‑150 (MQ=255)
                                                                                                                                         accaacaGGTACAGGGCCGTCGCTGTCGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGCCCCCTTCTACAAGACCACGAATCAACGTACAGGGTGCCAATTACCCGATCAAGGGCAACCCCGCGCCATGCGCTGGTGGTcgtcgtcgt            >  2:4502934/5‑150 (MQ=255)
                                                                                                                                           ccccaGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTAc          <  1:3698708/147‑1 (MQ=255)
                                                                                                                                           ccACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTAc          >  1:4056619/1‑150 (MQ=255)
                                                                                                                                           ccACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTAc          >  1:1086413/1‑150 (MQ=255)
                                                                                                                                           ccACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTAc          >  2:1656374/1‑150 (MQ=255)
                                                                                                                                           ccACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGACTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTAc          >  1:4119166/1‑150 (MQ=255)
                                                                                                                                            cacaGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTACt         >  2:7100337/1‑150 (MQ=255)
                                                                                                                                             acaGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTACtt        >  2:7427617/1‑150 (MQ=255)
                                                                                                                                             acaGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTACtt        >  2:2005326/1‑150 (MQ=255)
                                                                                                                                                 gTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGACTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTACTTTTcc    <  2:4119166/150‑1 (MQ=255)
                                                                                                                                                   aCAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTACTTTTCCAg  <  2:7287768/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTGTCTGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTACTTTTCCAG  >  NC_002947/5657814‑5658110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑