Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,862,970 C→G 5.0% R172G (CGG→GGG)  PP_5134 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,862,9700CG 5.0% 1212.9 / 37.0 352R172G (CGG→GGG) PP_5134hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (167/167);  new base G (10/8);  total (177/175)
Fisher's exact test for biased strand distribution p-value = 8.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 371 total aligned reads displayed.

TGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAGCGTTTG  >  NC_002947/5862821‑5863119
                                                                                                                                                     |                                                                                                                                                     
tGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGccc                                                                                                                                                       <  2:6241557/150‑1 (MQ=255)
    tgtgATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCt                                                                                                                                                   >  2:3773266/1‑150 (MQ=255)
    tgtgATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCt                                                                                                                                                   >  1:6193698/1‑150 (MQ=255)
     gtgATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCtt                                                                                                                                                  <  2:3654487/150‑1 (MQ=255)
       gATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTgg                                                                                                                                                <  2:2459547/150‑1 (MQ=255)
       gATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTgg                                                                                                                                                >  1:4005527/1‑150 (MQ=255)
         tCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCAAGCCGGGCAAGGGc                                                                                                                                              <  1:1013348/150‑1 (MQ=255)
              gcgcgcGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCAc                                                                                                                                         <  2:5595581/150‑1 (MQ=255)
              gcgcgcGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCAc                                                                                                                                         <  1:6382686/150‑1 (MQ=255)
              gcgcgcGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCAAGCCGTGCAAGGGCTTCAc                                                                                                                                         <  2:5671709/150‑1 (MQ=255)
                  gcgAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCAt                                                                                                                                     <  2:5931019/150‑1 (MQ=255)
                   cgAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCAtt                                                                                                                                    >  1:2974574/1‑150 (MQ=255)
                   cgAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCAtt                                                                                                                                    <  1:6965049/150‑1 (MQ=255)
                   cgAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCAtt                                                                                                                                    >  2:3956304/1‑150 (MQ=255)
                    gAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTg                                                                                                                                   >  1:4247572/1‑150 (MQ=255)
                     aGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTgg                                                                                                                                  >  1:4059102/1‑150 (MQ=255)
                      gCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGt                                                                                                                                 >  1:1883332/1‑150 (MQ=255)
                         ggTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACg                                                                                                                              <  1:1641640/150‑1 (MQ=255)
                           tGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGt                                                                                                                            >  1:253459/1‑150 (MQ=255)
                           tGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGt                                                                                                                            >  2:5985611/1‑150 (MQ=255)
                             cTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTc                                                                                                                          <  2:6374735/150‑1 (MQ=255)
                             cTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTc                                                                                                                          <  2:4720723/150‑1 (MQ=255)
                             cTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCAAGCCGGGCAAGGGCTTCAc                                                                                                                                         >  2:4441878/1‑135 (MQ=255)
                             cTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCAAGCCGGGCAAGGGCTTCAc                                                                                                                                         <  1:4441878/135‑1 (MQ=255)
                                   aaGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGc                                                                                                                    >  1:2049407/1‑150 (MQ=255)
                                   aaGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGc                                                                                                                    <  2:4928405/150‑1 (MQ=255)
                                   aaGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGGGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGc                                                                                                                    <  1:3070703/150‑1 (MQ=255)
                                     gTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGt                                                                                                                  <  2:6435779/150‑1 (MQ=255)
                                      tACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTg                                                                                                                 <  2:3920806/150‑1 (MQ=255)
                                         cgcgACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGcc                                                                                                              >  2:2134306/1‑150 (MQ=255)
                                               gaACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCa                                                                                                        <  2:3675317/150‑1 (MQ=255)
                                                 tcTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCaga                                                                                                      <  1:3507186/149‑1 (MQ=255)
                                                 aCTGGAAGTGCTGGCCAACTGCGGCACGCCGTTGCTGCCGGGGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCaga                                                                                                      <  2:6597253/150‑1 (MQ=255)
                                                 aCTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCaga                                                                                                      <  2:3558282/150‑1 (MQ=255)
                                                   tGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAgg                                                                                                    >  1:6655501/1‑150 (MQ=255)
                                                     gAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGAt                                                                                                  >  1:5504178/1‑150 (MQ=255)
                                                        gTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGt                                                                                               <  1:508825/150‑1 (MQ=255)
                                                        gTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGt                                                                                               <  2:1410369/150‑1 (MQ=255)
                                                          gCTGGCCAACGGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGa                                                                                             <  2:1951483/150‑1 (MQ=255)
                                                           cTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAg                                                                                            >  1:1219942/1‑150 (MQ=255)
                                                            tGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAgc                                                                                           >  2:5383065/1‑150 (MQ=255)
                                                              gCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAgcgc                                                                                         >  1:5409570/1‑150 (MQ=255)
                                                               ccAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGcc                                                                                        >  2:4320226/1‑150 (MQ=255)
                                                                 ttcTGCGGCAAGCCGTTGGTGCCGGTGCTCAAGTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGATTGGCGTTAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACTGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGt                                                                                      <  2:165936/148‑1 (MQ=255)
                                                                 aaCTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGt                                                                                      >  1:3385395/1‑150 (MQ=255)
                                                                   cTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGttt                                                                                    <  1:4599347/150‑1 (MQ=255)
                                                                   cTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGttt                                                                                    <  1:999584/150‑1 (MQ=255)
                                                                        gCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACg                                                                               >  2:5115344/1‑150 (MQ=255)
                                                                          aaGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGaa                                                                             >  2:2675658/1‑150 (MQ=255)
                                                                           aGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGaaa                                                                            <  2:582667/150‑1 (MQ=255)
                                                                           aGCCGGTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGaaa                                                                            <  1:849264/150‑1 (MQ=255)
                                                                             ccGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGc                                                                          >  2:418071/1‑150 (MQ=255)
                                                                              cGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGcc                                                                         <  1:6007854/150‑1 (MQ=255)
                                                                                  gctgcCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGgc                                                                     <  2:70037/150‑1 (MQ=255)
                                                                                     gcCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTg                                                                                   >  2:205148/1‑133 (MQ=255)
                                                                                       cGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTCTGTACGAAAGCCTGGCgctgc                                                                <  1:2893839/150‑1 (MQ=255)
                                                                                        ggTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCgctgct                                                               <  1:114283/150‑1 (MQ=255)
                                                                                             tCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCGGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTgg                                                          >  1:5201191/1‑150 (MQ=255)
                                                                                             tCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTgg                                                          >  2:2191996/1‑150 (MQ=255)
                                                                                             tCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTgg                                                          >  2:5424861/1‑150 (MQ=255)
                                                                                              cAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGa                                                         <  1:5688716/150‑1 (MQ=255)
                                                                                              cAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGa                                                         >  1:438829/1‑150 (MQ=255)
                                                                                                 cTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGaaaa                                                      >  2:669324/1‑150 (MQ=255)
                                                                                                  ttCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAc                                                     <  2:4588972/150‑1 (MQ=255)
                                                                                                  ttCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAc                                                     >  1:6891534/1‑150 (MQ=255)
                                                                                                    cgtcgCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAcgc                                                   <  1:1340780/150‑1 (MQ=255)
                                                                                                      tcgCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAcgccc                                                 <  1:7410936/150‑1 (MQ=255)
                                                                                                         ccagcagccagcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAcgcccgcc                                              >  2:3202266/1‑150 (MQ=255)
                                                                                                          cagcagccagcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACgcccgccc                                             >  2:3831941/1‑150 (MQ=255)
                                                                                                           agcagccagcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGcccgcccg                                            <  2:2568583/150‑1 (MQ=255)
                                                                                                             cagccagcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGgc                                          >  1:579705/1‑150 (MQ=255)
                                                                                                               gccagcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGgcgc                                        <  2:2386024/150‑1 (MQ=255)
                                                                                                                ccagcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCt                                       <  2:1129636/150‑1 (MQ=255)
                                                                                                                 cagcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTg                                      >  2:1276110/1‑150 (MQ=255)
                                                                                                                    caCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCaa                                   <  1:3703874/150‑1 (MQ=255)
                                                                                                                     aCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAAc                                  <  1:3678085/150‑1 (MQ=255)
                                                                                                                       cgcgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACgg                                >  1:2292201/1‑150 (MQ=255)
                                                                                                                        gcgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGc                               <  1:5725642/150‑1 (MQ=255)
                                                                                                                         cgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCt                              <  2:3272821/150‑1 (MQ=255)
                                                                                                                         cgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCt                              <  1:7246353/150‑1 (MQ=255)
                                                                                                                         cgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCt                              <  1:3831592/150‑1 (MQ=255)
                                                                                                                         cgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCt                              <  1:4569319/150‑1 (MQ=255)
                                                                                                                             ccTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATc                          >  2:5232449/1‑150 (MQ=255)
                                                                                                                              cTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATcg                         >  2:6907647/1‑150 (MQ=255)
                                                                                                                               tGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATcga                        <  1:502764/150‑1 (MQ=255)
                                                                                                                                gAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGt                                                                                               <  1:4909220/78‑1 (MQ=255)
                                                                                                                                  aTGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATcgacga                     <  2:7410933/150‑1 (MQ=255)
                                                                                                                                  aTGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATcgacga                     <  2:7277437/150‑1 (MQ=255)
                                                                                                                                     gCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACca                  <  1:6746109/150‑1 (MQ=255)
                                                                                                                                       gTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACcagc                >  1:7033040/1‑150 (MQ=255)
                                                                                                                                        tGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACcagca               >  1:2764392/1‑150 (MQ=255)
                                                                                                                                         gAAGCCCAAGCCGGGCAAGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGATGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACaagcag              >  2:3152608/1‑150 (MQ=255)
                                                                                                                                          aaGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAgg             <  1:6628522/150‑1 (MQ=255)
                                                                                                                                            gCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGca           >  2:1522429/1‑150 (MQ=255)
                                                                                                                                               cTTGCCCGGCTTGGGCTTCGCGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAg        <  1:475153/150‑1 (MQ=255)
                                                                                                                                               cTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAg        >  1:154169/1‑150 (MQ=255)
                                                                                                                                                    ccGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATcgac                       >  1:1773097/1‑130 (MQ=255)
                                                                                                                                                    ccGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATcgac                       <  2:1773097/130‑1 (MQ=255)
                                                                                                                                                    ccGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAGCGttt   >  2:4757513/1‑150 (MQ=255)
                                                                                                                                                    ccGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAGCGttt   <  2:4059005/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAGCGTTTG  >  NC_002947/5862821‑5863119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑