BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	733,649	A→T	6.1%	intergenic (+20/‑27)	PP_t09 → / → PP_t10	tRNA‑Lys/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	733,649	0	A	T	6.1%	530.6 / 13.5	177	intergenic (+20/‑27)	PP_t09/PP_t10	tRNA‑Lys/tRNA‑Pro
Reads supporting (aligned to +/- strand): ref base A (92/71); new base T (8/4); total (100/77)
Fisher's exact test for biased strand distribution p-value = 5.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

GCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACC  >  NC_002947/733501‑733797

  tttGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAaga                                                                                                                                                   <  2:3122198/150‑1 (MQ=255)

   ttGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAagag                                                                                                                                                  <  1:4198094/150‑1 (MQ=255)

   ttGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAagag                                                                                                                                                  <  2:126455/150‑1 (MQ=255)

       gAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGc                                                                                                                                              >  1:1610410/1‑150 (MQ=255)

       gAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATAACAAAGAGATGc                                                                                                                                              >  1:954928/1‑150 (MQ=255)

        aGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCTTAAGAACAGTTGGAAGAGCAGATGGCAATTAACCAATACGTCGAAGGATCGAAAACTACACTACCCACAATGTAGAAAAGGGCATATCAAAGAGATgac                                                                                                                                             >  1:1059974/1‑148 (MQ=17)

          cAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGACATGCCCt                                                                                                                                           >  1:3070735/1‑150 (MQ=255)

             gATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGCCCtttt                                                                                                                                        <  2:5166659/150‑1 (MQ=255)

               tGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCtttttt                                                                                                                                      <  1:1808913/150‑1 (MQ=255)

               tGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCtttttt                                                                                                                                      <  1:5444791/150‑1 (MQ=255)

                gTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCttttttt                                                                                                                                     <  2:1347314/150‑1 (MQ=255)

                gTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCttttttt                                                                                                                                     <  2:3015155/150‑1 (MQ=255)

                 tCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAATGGCAAGCACAAAGAGAAGACCtaggtttt                                                                                                                                    >  2:5566939/1‑143 (MQ=14)

                   aGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGa                                                                                                                                  >  2:840845/1‑150 (MQ=255)

                    ggAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGaa                                                                                                                                 <  2:6215013/150‑1 (MQ=255)

                        ttCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGTAacac                                                                                                                             >  1:4395332/1‑150 (MQ=255)

                           gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCg                                                                                                                          >  2:6120845/1‑150 (MQ=255)

                           gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCg                                                                                                                          <  1:5896265/150‑1 (MQ=255)

                            aaTCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCgg                                                                                                                         <  1:1245429/150‑1 (MQ=255)

                             aTCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCggg                                                                                                                        <  2:157126/150‑1 (MQ=255)

                               ccccTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGgt                                                                                                                      <  1:4012587/150‑1 (MQ=255)

                                cccTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGgtg                                                                                                                     >  1:3837432/1‑150 (MQ=255)

                                 ccTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCATTTTTTTGAAACAATCGGGgtgt                                                                                                                    >  1:6104131/1‑150 (MQ=255)

                                  cTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTa                                                                                                                   <  1:767579/150‑1 (MQ=255)

                                  cTCACCCCGACCATTTTCCGGGTCGTTAGATCAGTTGGTAGAGCAGTTTGCTTTTAACCCATTGGTCGTAGGTTAGAATCCTACTCTACCCACCATTTATAAAAGGGCATCTGAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTa                                                                                                                   <  2:2395491/150‑1 (MQ=255)

                                      ccccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTGAAACACTCGGGGTGTAGCGCa                                                                                                              <  2:4570717/150‑1 (MQ=255)

                                       cccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCa                                                                                                              <  2:6618006/150‑1 (MQ=255)

                                         cGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGt                                                                                                            <  1:6875844/150‑1 (MQ=255)

                                          gACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCAAATCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTc                                                                                                           >  1:3837774/1‑150 (MQ=255)

                                            ccATGTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGTCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTCAGAGATGCCCTTTTTTTTGAACCACTCGGGGTGTAGCGCAGTCCg                                                                                                         <  2:70036/150‑1 (MQ=21)

                                             cATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCggg                                                                                                                        >  1:5684899/1‑134 (MQ=255)

                                              aTTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGt                                                                                                       >  2:2222109/1‑150 (MQ=255)

                                                 ttCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAACAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAgc                                                                                                    <  2:2745590/150‑1 (MQ=255)

                                                     gggTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACCCGACCCACCCTGTAGAAAAGGGCATCTCCACGCGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAgcgcgc                                                                                                <  2:1934725/150‑1 (MQ=21)

                                                     gggTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACAAGGAACAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAgcgcgc                                                                                                >  1:3604152/1‑150 (MQ=255)

                                                           ttAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCtt                                                                                          >  2:4501466/1‑150 (MQ=255)

                                                           ttAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTGTTGAAACACTAGGGGTGTAGAGCAGTCCGGTCGCGCACCTGCtt                                                                                          >  2:4693032/1‑150 (MQ=21)

                                                            tAGCTCAGTTGGTAGAGCAGTTGGCTTTTTACCTCTGGTTAGTAGGTTCGAATCCTTCACGACCCACCCTGTAGAAAAGGGCCTCTCAAAGAGATGCACTTTTTTTTGAAACTCTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCttt                                                                                         <  1:6050168/150‑1 (MQ=25)

                                                             aGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCCCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTg                                                                                        <  2:2550143/150‑1 (MQ=255)

                                                                tCAGTTGGTAGAGCAGTTGGCTTTTAACCACTTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGACACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGa                                                                                     <  1:1011782/150‑1 (MQ=255)

                                                                tCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGa                                                                                     >  2:3939085/1‑150 (MQ=255)

                                                                   gTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCa                                                                                  >  2:4535028/1‑150 (MQ=255)

                                                                      ggTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGa                                                                               >  2:2698342/1‑150 (MQ=255)

                                                                             cAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCtt                                                                                          >  2:3686773/1‑132 (MQ=255)

                                                                             cAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCtt                                                                                          <  1:3686773/132‑1 (MQ=255)

                                                                                ttGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTACAAAAGGCCAAAACAAAGAGATGCCCTTTTTATTGAAACACTCGCGTTGACGCCCACTCCGGAAGCGCGACAGCTTTGGGCGCAGGATGTCAGGAGt                                                                     >  2:1791503/1‑150 (MQ=21)

                                                                                ttGGCTTTTAACCAATTGGTCGTAGGTACGAATCCTACACGACACACCATCTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTATTTGAAACAATCGGGGTGTAGCGCGGTACGGTATCGCGCATGCTTTGGGAGCAGGATGACAGGAGt                                                                     >  2:2894154/1‑150 (MQ=37)

                                                                                ttGGCTTTGAACCAAGTGGTCGTAGGTTCGAATCCTCATCGACCGACCCTGTAGCAAAGGGCATCTCCAAGCGATGCCCTTATTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGt                                                                     <  2:4153842/150‑1 (MQ=25)

                                                                                 tGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTACAAATGGCCATAACAAAGAGATGCCCTTTTTATAGAAACACACGGGTAGAACCGCACTCCGGAATCGCGCCTGATGTGGGAGCAGGATGTCAGGAgat                                                                    >  2:4892445/1‑148 (MQ=18)

                                                                                  ggCTTTTAAACAATTGGTCGTAGGTTCGTAGGCTACACGTCCCACCCTGTAGCATAGGGCACCTGTTAGCTCTGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTc                                                                   <  2:3880591/150‑1 (MQ=21)

                                                                                     ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGaa                                                                >  2:354724/1‑150 (MQ=255)

                                                                                     ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAAt                                                               >  2:1538877/1‑150 (MQ=255)

                                                                                      tttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGTGGTGTAGAGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAAt                                                               >  2:4882989/1‑150 (MQ=255)

                                                                                          aCCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATcccc                                                           >  1:6329127/1‑150 (MQ=255)

                                                                                           ccAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCt                                                          >  1:694816/1‑150 (MQ=255)

                                                                                            cAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTc                                                         >  2:64540/1‑150 (MQ=255)

                                                                                            cAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTc                                                         >  1:2236492/1‑150 (MQ=255)

                                                                                            cAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTc                                                         >  1:5479265/1‑150 (MQ=255)

                                                                                            cAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTc                                                         >  2:3274936/1‑150 (MQ=255)

                                                                                            cAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTc                                                         >  1:2933108/1‑150 (MQ=255)

                                                                                             aaTTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCa                                                        >  2:6936763/1‑150 (MQ=255)

                                                                                             aaTTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCa                                                        >  1:721741/1‑150 (MQ=255)

                                                                                              aTTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCAc                                                       >  2:5927365/1‑150 (MQ=255)

                                                                                                  gTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAAGGGAAAAACAAAGAGATGCCCTTTTTATTTAAACACTAGAGGTGAACCCAAGTCCGGAATCGCGCCTGCATAGAGAGAAGTATGTCAGGAGTACGAAACCCCTCAccaca                                                   >  2:6766357/1‑147 (MQ=16)

                                                                                                   tCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGa                                                  >  2:5320297/1‑150 (MQ=255)

                                                                                                     gTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCTGGATGTCAGGAGTTCGAATCCCCTCACCCCGAcc                                                >  2:814555/1‑150 (MQ=255)

                                                                                                      tAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGgtgt                                                                                                                    >  1:2321260/1‑81 (MQ=255)

                                                                                                      tAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGgtgt                                                                                                                    <  2:2321260/81‑1 (MQ=255)

                                                                                                            cGAATCCTACACGAACCACCATGTAGAAATGGGCATCTCAAAGAGATTCCCTTTTTTTTGAAACTCTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTg                                         <  2:2434367/150‑1 (MQ=255)

                                                                                                                tCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTc                                     <  1:64540/150‑1 (MQ=255)

                                                                                                                  cTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGt                                   >  1:324474/1‑150 (MQ=255)

                                                                                                                   tACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGtt                                  >  1:4560108/1‑150 (MQ=255)

                                                                                                                   tACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGtt                                  >  1:3335354/1‑150 (MQ=255)

                                                                                                                      acGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATc                                                              >  2:1064882/1‑119 (MQ=255)

                                                                                                                        gACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTc                             >  2:5231439/1‑150 (MQ=255)

                                                                                                                        gACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTc                             >  1:5353986/1‑150 (MQ=255)

                                                                                                                         aCCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCa                            <  1:6120845/150‑1 (MQ=255)

                                                                                                                          cccACCATGTAGAAAAGGGCATCTCAAATAGATGACCTTTTTTTTGAAACACTAGGGGTGTATCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGATTTAGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAg                           >  1:2305493/1‑150 (MQ=21)

                                                                                                                           ccaccaTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGGGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGt                          >  2:7101148/1‑150 (MQ=255)

                                                                                                                            caccaTCTAGAAAAGTGCATCTCAACGCGATGCACTTTTTTTTGAAACACTCGCGGAGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGtt                         <  2:1059974/150‑1 (MQ=18)

                                                                                                                               ttcaaaaaaaaGGGCATCTCTTTGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCGCAGTTGGt                      >  1:5008584/8‑150 (MQ=18)

                                                                                                                                aTGTAGACAAGGGCATCTCAAAGAGTTGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGATGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCATGAGTTCGAATCCCCTCACCCCTAACATTTTTGGGTCTTTAGCTCAGTTGGTa                     <  1:6257119/150‑1 (MQ=21)

                                                                                                                                aTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTa                     >  2:6805800/1‑150 (MQ=255)

                                                                                                                                aTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTa                     <  2:5846126/150‑1 (MQ=255)

                                                                                                                                      aaaaGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAg               >  1:2114334/1‑150 (MQ=255)

                                                                                                                                        aaGGGCATCTCAACGAGATGCCCTTTTTTTTGACACCCTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGtt             <  2:1610410/150‑1 (MQ=255)

                                                                                                                                         aGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTTGCTCAGTTGGTAGAGCAGTTg            >  1:1429167/1‑150 (MQ=255)

                                                                                                                                          gggCATCTCTTTGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg           >  1:3789789/1‑150 (MQ=255)

                                                                                                                                          gggCATCTCTTTGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg           >  2:4370113/1‑150 (MQ=255)

                                                                                                                                          gggCATCTCTTTGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg           >  2:654726/1‑150 (MQ=255)

                                                                                                                                          gggCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTCGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg           >  2:1367956/1‑150 (MQ=255)

                                                                                                                                          gggCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg           >  2:882803/1‑150 (MQ=255)

                                                                                                                                           ggCATCTCTTTGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGc          >  1:5258774/1‑150 (MQ=255)

                                                                                                                                           ggCATCTCTTTGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGc          >  2:2187971/1‑150 (MQ=255)

                                                                                                                                             cATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCtt        <  2:324474/150‑1 (MQ=255)

                                                                                                                                              ctctcCAAGATTTGCCCTTTTTTTTGCCAGTCTCGGGGTGTCGCGCAGTCCGGTTGCGAGCCTGCTCTGGGCGCAGGATGTCAGGTGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCttt       <  1:6591340/149‑1 (MQ=14)

                                                                                                                                               tctcAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTCGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCtttt      >  1:2199796/1‑150 (MQ=255)

                                                                                                                                                 tcAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTaa    >  1:1779928/1‑150 (MQ=255)

                                                                                                                                                  cAAAGAGATGCCCTTTTTTTTGAAACACACGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAc   >  1:293985/1‑150 (MQ=255)

                                                                                                                                                   aaaGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAcc  >  2:4412687/1‑150 (MQ=255)

GCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACC  >  NC_002947/733501‑733797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`