BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	100,747	A→T	5.1%	M111K (ATG→AAG)	PP_0095 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	100,747	0	A	T	5.1%	1195.1 / 32.1	347	M111K (ATG→AAG)	PP_0095	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (154/174); new base T (7/11); total (161/186)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 388 total aligned reads displayed.

AGGAGAAAAATGCGCGCTCCTTGTCGTTCAGCAGCCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAATGTGCAGCACGCTG  >  NC_002947/100598‑100896

 ggAGAAAAATGCGCGCTCCTTGTCGTTCAGCAGCCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCCGCGCCAATGATTACTTCGTCCTCGACGATGGCAccatccag                                                                                                                                                      >  2:5244631/1‑149 (MQ=255)

  gagaAAAATGCGCGCTCCTTGTCGTTCAGCAGCCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCatg                                                                                                                                                     >  1:342417/1‑150 (MQ=255)

  gagaAAAATGCGCGCTCCTTGTCGTTCAGCAGCCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCatg                                                                                                                                                     >  2:4057482/1‑150 (MQ=255)

        aaTGCGCGCTCCTTGTCGTTCAGCAGCCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGtg                                                                                                                                               <  2:2123452/150‑1 (MQ=255)

          tGCGCGCTCCTTGTCGTTCAGCAGCCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATCGCACCATCATGGATGGtgcc                                                                                                                                             <  1:4486242/150‑2 (MQ=255)

            cgcgcTCCTTGTCGTTCAGCAGCCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGtgctgc                                                                                                                                           <  1:4183118/150‑1 (MQ=255)

            cgcgcTCCTTGTCGTTCAGCAGCCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATAGCACCATCATGGATGGtgccat                                                                                                                                           <  1:2197173/150‑4 (MQ=255)

                  ccTTGTCGTTCAGCAGCCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATAc                                                                                                                                     <  2:3447792/150‑1 (MQ=255)

                        cGTTCAGCAGCCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACccacca                                                                                                                               <  2:3136295/150‑1 (MQ=255)

                        cGTTCAGCAGCCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACccacca                                                                                                                               <  2:7107860/150‑1 (MQ=255)

                           tCAGCAGCCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGa                                                                                                                            <  2:822884/150‑1 (MQ=255)

                              gcagcCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATAc                                                                                                                         >  1:7377937/1‑150 (MQ=255)

                               cagcCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACg                                                                                                                        <  1:435212/150‑1 (MQ=255)

                                agcCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACgg                                                                                                                       <  1:1386564/150‑1 (MQ=255)

                                  ccGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGtt                                                                                                                     <  2:6899200/150‑1 (MQ=255)

                                       ccTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCa                                                                                                                >  1:5677877/1‑150 (MQ=255)

                                         tGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAgg                                                                                                              <  2:3299510/150‑1 (MQ=255)

                                          gTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAggg                                                                                                             <  2:7008428/150‑1 (MQ=255)

                                          gTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAggg                                                                                                             <  2:6328083/150‑1 (MQ=255)

                                           tttCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGt                                                                                                            >  2:280046/1‑150 (MQ=255)

                                            ttCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCCGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTg                                                                                                           >  2:7261083/1‑150 (MQ=255)

                                             tCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGc                                                                                                          >  1:5706631/1‑150 (MQ=255)

                                             tCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGc                                                                                                          <  2:5079854/150‑1 (MQ=255)

                                              cACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCa                                                                                                         <  2:5381284/150‑1 (MQ=255)

                                               aCCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCatgatg                                                                                                                                                  >  1:4627428/1‑108 (MQ=255)

                                               aCCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCatgatg                                                                                                                                                  <  2:4627428/108‑1 (MQ=255)

                                               aCCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGATGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAg                                                                                                        >  2:4932105/1‑150 (MQ=255)

                                                ccGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGtgct                                                                                                                                             >  1:7035996/1‑112 (MQ=255)

                                                ccGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGc                                                                                                       <  2:4732212/150‑1 (MQ=255)

                                                ccGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGc                                                                                                       <  2:5022362/150‑1 (MQ=255)

                                                ccGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGc                                                                                                       <  1:856400/150‑1 (MQ=255)

                                                      tGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATgca                                                                                                 >  1:5509752/1‑150 (MQ=255)

                                                      tGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATgca                                                                                                 <  1:4711826/150‑1 (MQ=255)

                                                      tGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATgca                                                                                                 <  2:1714040/150‑1 (MQ=255)

                                                                cAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCtt                                                                                       <  1:32655/150‑1 (MQ=255)

                                                                     aGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGcc                                                                                  >  1:769527/1‑150 (MQ=255)

                                                                          cTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATg                                                                             <  2:4643375/150‑1 (MQ=255)

                                                                          cTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATg                                                                             <  1:4888002/150‑1 (MQ=255)

                                                                          cTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATg                                                                             <  1:2865417/150‑1 (MQ=255)

                                                                          cTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATg                                                                             <  1:5779460/150‑1 (MQ=255)

                                                                          cTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATg                                                                             <  1:6410458/150‑1 (MQ=255)

                                                                               cAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCAc                                                                        >  2:3822419/1‑150 (MQ=255)

                                                                                aGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACt                                                                       >  1:6000018/1‑150 (MQ=255)

                                                                                  gCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTtc                                                                     >  1:1870631/1‑150 (MQ=255)

                                                                                  gCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTtc                                                                     >  1:2250806/1‑150 (MQ=255)

                                                                                     tttGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTtcgtc                                                                  <  2:3909965/150‑1 (MQ=255)

                                                                                         cccGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCg                                                              <  1:3098140/150‑1 (MQ=255)

                                                                                         cccGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCg                                                              >  2:1947275/1‑150 (MQ=255)

                                                                                          ccGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCga                                                             <  2:576626/150‑1 (MQ=255)

                                                                                          ccGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCga                                                             >  2:1677805/1‑150 (MQ=255)

                                                                                           cggcggCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCgat                                                            >  1:2679525/1‑150 (MQ=255)

                                                                                            ggcggcACCAGGCTGCCAGCGCCAATGATGACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCgatg                                                           <  1:3796386/150‑1 (MQ=255)

                                                                                                 cACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAg                                                      <  2:7377937/150‑1 (MQ=255)

                                                                                                  aCCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATa                                                                                                                                      <  2:2577434/69‑1 (MQ=255)

                                                                                                  aCCAGGCTGCCAGCGCCAATGATTACTTCGACCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGc                                                     >  2:5517917/1‑150 (MQ=255)

                                                                                                   ccAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCg                                                    <  1:7199664/150‑1 (MQ=255)

                                                                                                     aGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCggg                                                  >  1:6718709/1‑150 (MQ=255)

                                                                                                         tGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAAc                                              >  1:1986820/1‑150 (MQ=255)

                                                                                                         tGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAAc                                              >  1:1821782/1‑150 (MQ=255)

                                                                                                             aGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTc                                          >  1:5005575/1‑150 (MQ=255)

                                                                                                               cgcCAATGAGTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCg                                        <  2:3217484/150‑1 (MQ=255)

                                                                                                                gcCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCgg                                       <  2:6565092/150‑1 (MQ=255)

                                                                                                                  cAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGt                                     >  2:854175/1‑150 (MQ=255)

                                                                                                                  cAATCATTACTACGTCCTCGACGATGGCAACATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGt                                     <  2:4444039/150‑1 (MQ=255)

                                                                                                                   aaTGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGtt                                    >  1:4447559/1‑150 (MQ=255)

                                                                                                                     tGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGa                                  <  1:1187788/150‑1 (MQ=255)

                                                                                                                      gATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGaa                                 <  2:5668428/150‑1 (MQ=255)

                                                                                                                      gATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGaa                                 <  2:1155106/150‑1 (MQ=255)

                                                                                                                      gATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACACACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGaa                                 >  1:689906/1‑150 (MQ=255)

                                                                                                                       aTTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGaaa                                <  2:2963292/150‑1 (MQ=255)

                                                                                                                       aTTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGaaa                                <  1:5069755/150‑1 (MQ=255)

                                                                                                                       aTTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGaaa                                <  1:6148131/150‑1 (MQ=255)

                                                                                                                          aCTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGAACAGCGCGAAACCGTCCGGGATGAAAGGc                             <  2:4898993/150‑1 (MQ=255)

                                                                                                                           cTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGcc                            >  2:5877756/1‑150 (MQ=255)

                                                                                                                            ttCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAgg                                                                                                              <  1:1669571/67‑1 (MQ=255)

                                                                                                                            ttCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGccc                           >  2:158311/1‑150 (MQ=255)

                                                                                                                            ttCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGccc                           >  2:2391392/1‑150 (MQ=255)

                                                                                                                             tCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCg                          <  2:3554945/150‑1 (MQ=255)

                                                                                                                               gTCCTCGACGATGGCACCATCCATGAGGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGGGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCGCTTCGTCGCCGATGCTCAGGGGGAAGCCGTCCGGGTGGTAAGGCCgggg                        >  2:6246273/1‑149 (MQ=255)

                                                                                                                                  cTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTg                     >  2:1431234/1‑150 (MQ=255)

                                                                                                                                   tCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTgg                    <  2:6468307/150‑1 (MQ=255)

                                                                                                                                     gacgaTGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGt                  >  1:7316738/1‑150 (MQ=255)

                                                                                                                                      tgggcagCACCATCATGGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTa                 >  1:2796845/7‑150 (MQ=255)

                                                                                                                                      acgaTGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTa                 <  1:3700940/150‑1 (MQ=255)

                                                                                                                                      acgaTGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTa                 >  1:3231524/1‑150 (MQ=255)

                                                                                                                                        gaTGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAAt               >  1:5180467/1‑150 (MQ=255)

                                                                                                                                        atgggCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAAACGTCCGGGTTGAAAGGCCCGGCGTGGGTAAt               >  2:650290/4‑150 (MQ=255)

                                                                                                                                         gcagCACCATCATGGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCg                                        >  1:2360535/4‑124 (MQ=255)

                                                                                                                                         aTGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAAtg              >  1:1097087/1‑150 (MQ=255)

                                                                                                                                         aTGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAAtg              <  2:6011560/150‑1 (MQ=255)

                                                                                                                                         aTGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGACGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAAtg              >  2:4278752/1‑150 (MQ=255)

                                                                                                                                          tGGCACCATCCATGATGGTGCTGCCCATACCCCCCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAAtgt             <  2:7247613/150‑1 (MQ=255)

                                                                                                                                            gCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAATGTgc           <  1:2074875/150‑1 (MQ=255)

                                                                                                                                            gCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAATGTgc           <  2:1392796/150‑1 (MQ=255)

                                                                                                                                             cACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAATGTgca          <  2:611487/150‑1 (MQ=255)

                                                                                                                                              aCCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAATGTgcag         <  2:6832523/150‑1 (MQ=255)

                                                                                                                                                catccatGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAATGTgcagca       >  1:3207537/1‑150 (MQ=255)

                                                                                                                                                 atccatGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAATGTGCAGCAc      >  1:918081/1‑150 (MQ=255)

                                                                                                                                                   ccatGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAATGTGCAGCACGc    >  1:2583867/1‑150 (MQ=255)

                                                                                                                                                     atgatgGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAATGTGCAGCACGCTg  >  1:1222297/1‑150 (MQ=255)

AGGAGAAAAATGCGCGCTCCTTGTCGTTCAGCAGCCGGGCCTGTTTCACCGGACTGCCCATGTACAGGTAGCCACTGACCAGGCGTTTGCCCGGCGGCACCAGGCTGCCAGCGCCAATGATTACTTCGTCCTCGACGATGGCACCATCCATGATGGTGCTGCCCATACCCACCAGGATACGGTTGCCCAGGGTGCAGCCATGCAGCATCACCTTGTGCCCGATGGTCACTTCGTCGCCGATGATCAGCGGGAAACCGTCCGGGTTGAAAGGCCCGGCGTGGGTAATGTGCAGCACGCTG  >  NC_002947/100598‑100896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`