BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	143,591	T→C	5.3%	L14P (CTG→CCG)	PP_0136 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	143,591	0	T	C	5.3%	992.6 / 26.4	302	L14P (CTG→CCG)	PP_0136	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (156/126); new base C (7/9); total (166/136)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

Only 100 of 313 total aligned reads displayed.

GCTTCGGTGGTATCGCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCG  >  NC_002947/143442‑143740

       tggtATCGCGGGCGCTGCTACCGGTATCGCCACGATCCTGTTCATGGTCTTCCTGGGGTTGTGTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGggc                                                                                                                                                <  2:6027999/150‑1 (MQ=255)

       tggtATCGCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGggc                                                                                                                                                <  1:6683169/150‑1 (MQ=255)

       tggtATCGCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGggc                                                                                                                                                <  2:1712581/150‑1 (MQ=255)

       tggtATCGCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGggc                                                                                                                                                <  2:4177225/150‑1 (MQ=255)

         gtATCGCGGGCGCTGCTACAGGTATCGACAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTCTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGggcgg                                                                                                                                              <  2:5419706/150‑1 (MQ=255)

           ctCGCGGGCGCGGCGACAGGTATCGCCAAGATCCTGTACAGTGTCTTCGTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGCCa                                                                                                                                            <  2:5757266/149‑1 (MQ=255)

                    gctgctACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGcggcgg                                                                                                                                   >  1:6213663/1‑150 (MQ=255)

                      tgctACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGcc                                                                                                                                 >  1:7328469/1‑150 (MQ=255)

                       gctACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCa                                                                                                                                >  2:2421294/1‑150 (MQ=255)

                         tACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATg                                                                                                                              >  2:7329216/1‑150 (MQ=255)

                           cAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATggc                                                                                                                            <  1:1606893/150‑1 (MQ=255)

                            aGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGCCGCCGCGCTGCCGCCCAGCAGCAGGGCGGCGACCACggct                                                                                                                           <  2:5209819/150‑2 (MQ=37)

                              gTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATggcggc                                                                                                                         >  1:7394331/1‑150 (MQ=255)

                                aTCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCa                                                                                                                       <  2:2692880/150‑1 (MQ=255)

                                 tCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGCCGCCGCGCTGCCGCCCAGCAGCAGGgcggcgac                                                                                                                                  >  1:4904865/1‑136 (MQ=38)

                                   gCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATg                                                                                                                    >  2:1983226/1‑150 (MQ=255)

                                   gCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATg                                                                                                                    >  2:491463/1‑150 (MQ=255)

                                    ccAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAAt                                                                                                                     >  2:381179/1‑148 (MQ=255)

                                    ccAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGa                                                                                                                   >  2:4639555/1‑150 (MQ=255)

                                    ccAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGa                                                                                                                   >  1:5528473/1‑150 (MQ=255)

                                     cAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGAc                                                                                                                  >  1:3157363/1‑150 (MQ=255)

                                        gATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGc                                                                                                               >  1:3737679/1‑150 (MQ=255)

                                              gTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGtt                                                                                                         >  1:4191915/1‑150 (MQ=255)

                                              gTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGtt                                                                                                         >  2:3073489/1‑150 (MQ=255)

                                               ttCTTTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTc                                                                                                        <  2:5061576/150‑1 (MQ=255)

                                               ttCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTc                                                                                                        >  1:5775237/1‑150 (MQ=255)

                                                 cATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCgg                                                                                                      <  2:3537572/150‑1 (MQ=255)

                                                 cATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCgg                                                                                                      <  1:3331724/150‑1 (MQ=255)

                                                 cATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCgg                                                                                                      <  1:86091/150‑1 (MQ=255)

                                                  cttGTCGTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGCCGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCggg                                                                                                     <  2:3886569/149‑1 (MQ=255)

                                                  aTTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGAAGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCggg                                                                                                     <  2:1142020/150‑1 (MQ=255)

                                                   ttGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGt                                                                                                    <  1:4428406/150‑1 (MQ=255)

                                                   ttGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGt                                                                                                    >  1:2427190/1‑150 (MQ=255)

                                                      tCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGa                                                                                                 >  2:6928230/1‑150 (MQ=255)

                                                          ccTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCaa                                                                                             >  1:6530862/1‑150 (MQ=255)

                                                            tGGTGTTGTTTGTAGCCTCCTTGTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACt                                                                                           >  2:6904479/1‑150 (MQ=255)

                                                            tGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACt                                                                                           >  1:980272/1‑150 (MQ=255)

                                                            tGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACt                                                                                           >  1:1154632/1‑150 (MQ=255)

                                                             ggTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTg                                                                                          <  1:2237881/150‑1 (MQ=255)

                                                               tgttgtTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgct                                                                                        <  2:6394163/150‑1 (MQ=255)

                                                                gttgttTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctc                                                                                       <  1:1652271/150‑1 (MQ=255)

                                                                 ttgtttgtAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctcg                                                                                      >  1:1022575/1‑150 (MQ=255)

                                                                 ttgtttgtAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctcg                                                                                      >  1:1373715/1‑150 (MQ=255)

                                                                    tttgtAGCCTCCTTCGTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctcggct                                                                                   <  2:3315175/150‑1 (MQ=255)

                                                                     ttgtAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctcggctc                                                                                  <  1:4859535/150‑1 (MQ=255)

                                                                     ttgtAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctcggctc                                                                                  <  1:4994449/150‑1 (MQ=255)

                                                                         aGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGAc                                                                              <  1:3423646/150‑1 (MQ=255)

                                                                           cctcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGAccc                                                                            <  2:2244452/150‑1 (MQ=255)

                                                                            ctcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCg                                                                           <  1:5920139/150‑1 (MQ=255)

                                                                            ctcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCg                                                                           >  1:6616905/1‑150 (MQ=255)

                                                                             tcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCgg                                                                          >  1:5472474/1‑150 (MQ=255)

                                                                              cctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGa                                                                         >  1:3529985/1‑150 (MQ=255)

                                                                                ttcttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAGGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGt                                                                       >  1:7122579/1‑150 (MQ=255)

                                                                                 tcttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTa                                                                      >  1:5989075/1‑150 (MQ=255)

                                                                                   ttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTAcc                                                                    >  1:1468375/1‑150 (MQ=255)

                                                                                   ttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTAcc                                                                    >  1:4656335/1‑150 (MQ=255)

                                                                                     cttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCgg                                                                  <  2:5007833/150‑1 (MQ=255)

                                                                                          gACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAAc                                                             >  2:1745005/1‑150 (MQ=255)

                                                                                           aCGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAAcc                                                            >  2:5150950/1‑150 (MQ=255)

                                                                                              cgGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTgg                                                         <  1:6503303/150‑1 (MQ=255)

                                                                                                 aCGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAg                                                      <  1:1250988/150‑1 (MQ=255)

                                                                                                   gAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGa                                                    >  1:2979432/1‑150 (MQ=255)

                                                                                                      gTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGacac                                                 >  2:1874122/1‑150 (MQ=255)

                                                                                                       ttGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACAcc                                                >  1:2002030/1‑150 (MQ=255)

                                                                                                        tGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACAccc                                               >  2:5888729/1‑150 (MQ=255)

                                                                                                         gAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCt                                              >  1:5796840/1‑150 (MQ=255)

                                                                                                           aGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCg                                            >  2:5351418/1‑150 (MQ=255)

                                                                                                           aGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCACCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCg                                            >  2:2134009/1‑150 (MQ=255)

                                                                                                                 gCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCg                                      >  1:4087366/1‑150 (MQ=255)

                                                                                                                  cAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGa                                     <  1:4733813/150‑1 (MQ=255)

                                                                                                                   aGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAg                                    <  2:2365549/150‑1 (MQ=255)

                                                                                                                   aGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAg                                    <  2:4448629/150‑1 (MQ=255)

                                                                                                                    ggAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAgg                                   >  2:798785/1‑150 (MQ=255)

                                                                                                                     gAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACCCCCTCGAAGGCGAGGa                                  >  1:6980428/1‑150 (MQ=255)

                                                                                                                     gAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGa                                  >  2:562865/1‑150 (MQ=255)

                                                                                                                     gAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGa                                  >  1:388220/1‑150 (MQ=255)

                                                                                                                       aGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAAc                                <  1:2038845/150‑1 (MQ=255)

                                                                                                                       aGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAAc                                <  1:6811146/150‑1 (MQ=255)

                                                                                                                         cTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGc                              >  2:1587583/1‑150 (MQ=255)

                                                                                                                         cTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGc                              <  2:3226795/150‑1 (MQ=255)

                                                                                                                          ttCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGcc                             >  2:7122123/1‑150 (MQ=255)

                                                                                                                          ttCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGcc                             >  2:1369130/1‑150 (MQ=255)

                                                                                                                             gTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGc                          <  2:1257664/150‑1 (MQ=255)

                                                                                                                               gccgCCATGGCCGCCGCGCTGCCGCCCAGCAGCAGGGCGGCGACCACGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGaa                                          <  2:6607263/129‑1 (MQ=21)

                                                                                                                                 cgCCATGGCCGCCGCGCTGCCGCCCAGCAGCAGGGCGGCGACCACGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGa                      >  1:1974513/2‑150 (MQ=25)

                                                                                                                                 aGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGa                      >  1:6344727/1‑150 (MQ=255)

                                                                                                                                   ccGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGAtt                    <  1:2699634/150‑1 (MQ=255)

                                                                                                                                     gTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTgg                  >  2:3469516/1‑150 (MQ=255)

                                                                                                                                      tGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTggg                 <  1:4836250/150‑1 (MQ=255)

                                                                                                                                       ggTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggt                <  1:1745005/150‑1 (MQ=255)

                                                                                                                                       ggTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggt                <  1:6254999/150‑1 (MQ=255)

                                                                                                                                         tCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggtgg              <  2:3004799/150‑1 (MQ=255)

                                                                                                                                         ccgccgcGCTGCCGCCCAGCAGCAGGGCGGCGACCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggtgg              >  2:2714865/2‑150 (MQ=38)

                                                                                                                                          cgccgcCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggtggt             <  2:4812336/150‑1 (MQ=255)

                                                                                                                                           gccgcgCTGCCGCCCAGCAGCAGGGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCgg                                                                  >  2:6101521/1‑96 (MQ=37)

                                                                                                                                           gccgcgCTGCCGCCCAGCAGCAGGGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCgg                                                                  <  1:6101521/96‑1 (MQ=37)

                                                                                                                                           gccgccCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGaa                                          <  2:6650297/120‑1 (MQ=255)

                                                                                                                                            ccgccCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCa           >  2:1015624/1‑150 (MQ=255)

                                                                                                                                                  tgctgctgGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGa     <  1:2832913/150‑1 (MQ=255)

                                                                                                                                                    ctgctgGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGAcc   <  1:7247283/150‑1 (MQ=255)

GCTTCGGTGGTATCGCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCG  >  NC_002947/143442‑143740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`